Restriction map for
pF1KB6927
(Circular) (Six-base) MAPSORT of: pF1KB6927.seq Check: 2827 from: 1 to: 3734
pF1KB6927 3734 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 772 772
Size: 3734
Acc65I G'GTAC_C
Cuts at: 750 750
Size: 3734
AclI AA'CG_TT
Cuts at: 3574 3574
Size: 3734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1559 1991 2738 3006 607
Size: 952 432 747 268 1335
Fragments arranged by size:
1335 952 747 432 268
AflIII A'CryG_T
Cuts at: 146 1086 2190 3167 146
Size: 940 1104 977 713
Fragments arranged by size:
1104 977 940 713
AgeI A'CCGG_T
Cuts at: 2122 2122
Size: 3734
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 643 675 643
Size: 32 3702
AlwNI CAG_nnn'CTG
Cuts at: 210 346 1039 2606 210
Size: 136 693 1567 1338
Fragments arranged by size:
1567 1338 693 136
ApaLI G'TGCA_C
Cuts at: 2504 2504
Size: 3734
ApoI r'AATT_y
Cuts at: 738 3087 3343 738
Size: 2349 256 1129
Fragments arranged by size:
2349 1129 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
AvaI C'yCGr_G
Cuts at: 152 169 467 722 754 152
Size: 17 298 255 32 3132
Fragments arranged by size:
3132 298 255 32 17
AvrII C'CTAG_G
Cuts at: 414 414
Size: 3734
BamHI G'GATC_C
Cuts at: 759 759
Size: 3734
BanI G'GyrC_C
Cuts at: 686 750 1164 1412 1447 686
Size: 64 414 248 35 2973
Fragments arranged by size:
2973 414 248 64 35
BanII G_rGCy'C
Cuts at: 167 321 604 748 1778 167
Size: 154 283 144 1030 2123
Fragments arranged by size:
2123 1030 283 154 144
BbeI G_GCGC'C
Cuts at: 690 1168 1416 690
Size: 478 248 3008
Fragments arranged by size:
3008 478 248
BciVI GTATCCnnnnn_n'
Cuts at: 1632 2393 3734 1632
Size: 761 1341 1632
Fragments arranged by size:
1632 1341 761
BclI T'GATC_A
Cuts at: 1254 1254
Size: 3734
BglI GCCn_nnn'nGGC
Cuts at: 132 343 503 132
Size: 211 160 3363
Fragments arranged by size:
3363 211 160
BglII A'GATC_T
Cuts at: 1249 3286 1249
Size: 2037 1697
BlpI GC'TnA_GC
Cuts at: 846 846
Size: 3734
Bme1580I G_kGCm'C
Cuts at: 1359 1452 2508 1359
Size: 93 1056 2585
Fragments arranged by size:
2585 1056 93
BmgBI CAC'GTC
Cuts at: 147 147
Size: 3734
BmrI ACTGGGnnnn_n'
Cuts at: 946 1027 1362 3506 946
Size: 81 335 2144 1174
Fragments arranged by size:
2144 1174 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 465 2101 465
Size: 1636 2098
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 163 205 661 157
Size: 6 42 456 3230
Fragments arranged by size:
3230 456 42 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 910 1789 2281 2579 493
Size: 417 879 492 298 1648
Fragments arranged by size:
1648 879 492 417 298
BsaI GGTCTCn'nnnn_
Cuts at: 3438 3438
Size: 3734
BsaAI yAC'GTr
Cuts at: 1087 1087
Size: 3734
BsaBI GATnn'nnATC
Cuts at: 6 1248 3285 6
Size: 1242 2037 455
Fragments arranged by size:
2037 1242 455
BsaHI Gr'CG_yC
Cuts at: 687 1165 1413 2115 3611 687
Size: 478 248 702 1496 810
Fragments arranged by size:
1496 810 702 478 248
BsaWI w'CCGG_w
Cuts at: 923 1444 2122 2396 2543 2954 923
Size: 521 678 274 147 411 1703
Fragments arranged by size:
1703 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 643 673 643
Size: 30 3704
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 658 157
Size: 501 3233
BseYI C'CCAG_C
Cuts at: 129 1159 1917 2494 2990 129
Size: 1030 758 577 496 873
Fragments arranged by size:
1030 873 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1276 1322 2530 104
Size: 1172 46 1208 1308
Fragments arranged by size:
1308 1208 1172 46
BsiHKAI G_wGCw'C
Cuts at: 167 748 1526 1716 2508 167
Size: 581 778 190 792 1393
Fragments arranged by size:
1393 792 778 581 190
Bsp1286I G_dGCh'C
Cuts at: 167 321 604 748 1359 1452 1526 1716 1778
Size: 154 283 144 611 93 74 190 62
Cuts at: 1778 2508 167
Size: 730 1393
Fragments arranged by size:
1393 730 611 283 190 154 144 93
74 62
BspEI T'CCGG_A
Cuts at: 2954 2954
Size: 3734
BspHI T'CATG_A
Cuts at: 2910 2910
Size: 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 784 998 1300 1681 784
Size: 214 302 381 2837
Fragments arranged by size:
2837 381 302 214
BsrBI CCG'CTC
Cuts at: 50 141 174 2026 2080 3564 3729 50
Size: 91 33 1852 54 1484 165 55
Fragments arranged by size:
1852 1484 165 91 55 54 33
BsrDI GCAATG_nn'
Cuts at: 1646 1646
Size: 3734
BsrFI r'CCGG_y
Cuts at: 298 683 1732 1913 2122 298
Size: 385 1049 181 209 1910
Fragments arranged by size:
1910 1049 385 209 181
BssSI C'ACGA_G
Cuts at: 2005 2363 2005
Size: 358 3376
BstAPI GCAn_nnn'nTGC
Cuts at: 210 1111 210
Size: 901 2833
BstBI TT'CG_AA
Cuts at: 2095 2095
Size: 3734
BstXI CCAn_nnnn'nTGG
Cuts at: 387 387
Size: 3734
BstYI r'GATC_y
Cuts at: 1 311 759 1226 1249 1584 1830 2842 2928
Size: 310 448 467 23 335 246 1012 86
Cuts at: 2928 2940 3286 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 448 346 335 310 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 498 107
Size: 391 3343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1684 1865 2028 1684
Size: 181 163 3390
Fragments arranged by size:
3390 181 163
BtsI GCAGTG_nn'
Cuts at: 977 977
Size: 3734
DraI TTT'AAA
Cuts at: 733 733
Size: 3734
DrdI GACnn_nn'nnGTC
Cuts at: 1440 2298 1440
Size: 858 2876
EaeI y'GGCC_r
Cuts at: 1319 1493 1884 1911 3256 1319
Size: 174 391 27 1345 1797
Fragments arranged by size:
1797 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1319 1319
Size: 3734
EarI CTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1942 2252 2398 3544 1942
Size: 310 146 1146 2132
Fragments arranged by size:
2132 1146 310 146
EcoICRI GAG'CTC
Cuts at: 165 746 165
Size: 581 3153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 163 205 607 661 1559 1991 2738 3006
Size: 6 42 402 54 898 432 747 268
Cuts at: 3006 157
Size: 885
Fragments arranged by size:
898 885 747 432 402 268 54 42
6
EcoO109I rG'GnC_Cy
Cuts at: 216 411 873 216
Size: 195 462 3077
Fragments arranged by size:
3077 462 195
EcoRI G'AATT_C
Cuts at: 738 738
Size: 3734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1753 1753
Size: 3734
FspI TGC'GCA
Cuts at: 1515 1515
Size: 3734
HaeII r_GCGC'y
Cuts at: 308 690 1168 1416 2438 3417 308
Size: 382 478 248 1022 979 625
Fragments arranged by size:
1022 979 625 478 382 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1576 1606 1608 1638 1576
Size: 30 2 30 3672
Fragments arranged by size:
3672 30 30 2
HincII GTy'rAC
Cuts at: 773 773
Size: 3734
KasI G'GCGC_C
Cuts at: 686 1164 1412 686
Size: 478 248 3008
Fragments arranged by size:
3008 478 248
KpnI G_GTAC'C
Cuts at: 754 754
Size: 3734
MluI A'CGCG_T
Cuts at: 3167 3167
Size: 3734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2405 2589 2405
Size: 184 3550
MscI TGG'CCA
Cuts at: 1495 1495
Size: 3734
MslI CAynn'nnrTG
Cuts at: 1723 1850 1723
Size: 127 3607
MspA1I CmG'CkG
Cuts at: 129 188 845 1159 1519 2532 2777 129
Size: 59 657 314 360 1013 245 1086
Fragments arranged by size:
1086 1013 657 360 314 245 59
NaeI GCC'GGC
Cuts at: 685 1915 685
Size: 1230 2504
NarI GG'CG_CC
Cuts at: 687 1165 1413 687
Size: 478 248 3008
Fragments arranged by size:
3008 478 248
NcoI C'CATG_G
Cuts at: 107 498 107
Size: 391 3343
NgoMIV G'CCGG_C
Cuts at: 683 1913 683
Size: 1230 2504
NspI r_CATG'y
Cuts at: 787 1287 2194 787
Size: 500 907 2327
Fragments arranged by size:
2327 907 500
PasI CC'CwG_GG
Cuts at: 380 478 597 380
Size: 98 119 3517
Fragments arranged by size:
3517 119 98
PciI A'CATG_T
Cuts at: 2190 2190
Size: 3734
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 643 675 2873 2905 643
Size: 32 2198 32 1472
Fragments arranged by size:
2198 1472 32 32
PspXI vC'TCGA_Gb
Cuts at: 169 169
Size: 3734
PstI C_TGCA'G
Cuts at: 215 236 781 1466 215
Size: 21 545 685 2483
Fragments arranged by size:
2483 685 545 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3734
PvuII CAG'CTG
Cuts at: 1159 1519 1159
Size: 360 3374
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3734
SacI G_AGCT'C
Cuts at: 167 748 167
Size: 581 3153
SalI G'TCGA_C
Cuts at: 771 771
Size: 3734
SapI GCTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3734
ScaI AGT'ACT
Cuts at: 3251 3251
Size: 3734
SfcI C'TryA_G
Cuts at: 33 211 232 777 1462 2455 2646 33
Size: 178 21 545 685 993 191 1121
Fragments arranged by size:
1121 993 685 545 191 178 21
SfoI GGC'GCC
Cuts at: 688 1166 1414 688
Size: 478 248 3008
Fragments arranged by size:
3008 478 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
SgrAI Cr'CCGG_yG
Cuts at: 683 683
Size: 3734
SmaI CCC'GGG
Cuts at: 154 469 756 154
Size: 315 287 3132
Fragments arranged by size:
3132 315 287
SmlI C'TyrA_G
Cuts at: 169 508 722 889 1804 2296 2558 169
Size: 339 214 167 915 492 262 1345
Fragments arranged by size:
1345 915 492 339 262 214 167
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3734
SphI G_CATG'C
Cuts at: 787 1287 787
Size: 500 3234
StuI AGG'CCT
Cuts at: 506 506
Size: 3734
StyI C'CwwG_G
Cuts at: 107 332 414 498 713 868 1222 107
Size: 225 82 84 215 155 354 2619
Fragments arranged by size:
2619 354 225 215 155 84 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 421 1317 1450 1909 2118 421
Size: 896 133 459 209 2037
Fragments arranged by size:
2037 896 459 209 133
TatI w'GTAC_w
Cuts at: 3249 3249
Size: 3734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 3702
Tth111I GACn'n_nGTC
Cuts at: 1531 1531
Size: 3734
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 339 1229 114
Size: 225 890 2619
Fragments arranged by size:
2619 890 225
XhoI C'TCGA_G
Cuts at: 169 722 169
Size: 553 3181
XmaI C'CCGG_G
Cuts at: 152 467 754 152
Size: 315 287 3132
Fragments arranged by size:
3132 315 287
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI
SwaI XmnI ZraI