Restriction map for
pF1KB6929
(Circular) (Six-base) MAPSORT of: pF1KB6929.seq Check: 9665 from: 1 to: 3734
pF1KB6929 3734 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 476 476
Size: 3734
AccI GT'mk_AC
Cuts at: 772 772
Size: 3734
Acc65I G'GTAC_C
Cuts at: 600 750 600
Size: 150 3584
AclI AA'CG_TT
Cuts at: 3574 3574
Size: 3734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1559 1991 2738 3006 1559
Size: 432 747 268 2287
Fragments arranged by size:
2287 747 432 268
AflIII A'CryG_T
Cuts at: 1086 2190 3167 1086
Size: 1104 977 1653
Fragments arranged by size:
1653 1104 977
AgeI A'CCGG_T
Cuts at: 2122 2122
Size: 3734
AhdI GACnn_n'nnGTC
Cuts at: 335 335
Size: 3734
AlwNI CAG_nnn'CTG
Cuts at: 725 1039 2606 725
Size: 314 1567 1853
Fragments arranged by size:
1853 1567 314
ApaI G_GGCC'C
Cuts at: 517 640 517
Size: 123 3611
ApaLI G'TGCA_C
Cuts at: 484 2504 484
Size: 2020 1714
ApoI r'AATT_y
Cuts at: 738 3087 3343 738
Size: 2349 256 1129
Fragments arranged by size:
2349 1129 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
AvaI C'yCGr_G
Cuts at: 169 177 754 169
Size: 8 577 3149
Fragments arranged by size:
3149 577 8
BamHI G'GATC_C
Cuts at: 164 759 164
Size: 595 3139
BanI G'GyrC_C
Cuts at: 191 600 750 1164 1412 1447 191
Size: 409 150 414 248 35 2478
Fragments arranged by size:
2478 414 409 248 150 35
BanII G_rGCy'C
Cuts at: 517 640 661 680 748 1778 517
Size: 123 21 19 68 1030 2473
Fragments arranged by size:
2473 1030 123 68 21 19
BbeI G_GCGC'C
Cuts at: 195 1168 1416 195
Size: 973 248 2513
Fragments arranged by size:
2513 973 248
BciVI GTATCCnnnnn_n'
Cuts at: 1632 2393 3734 1632
Size: 761 1341 1632
Fragments arranged by size:
1632 1341 761
BclI T'GATC_A
Cuts at: 1254 1254
Size: 3734
BglII A'GATC_T
Cuts at: 1249 3286 1249
Size: 2037 1697
BlpI GC'TnA_GC
Cuts at: 846 846
Size: 3734
Bme1580I G_kGCm'C
Cuts at: 488 517 640 1359 1452 2508 488
Size: 29 123 719 93 1056 1714
Fragments arranged by size:
1714 1056 719 123 93 29
BmgBI CAC'GTC
Cuts at: 151 151
Size: 3734
BmrI ACTGGGnnnn_n'
Cuts at: 946 1027 1362 3506 946
Size: 81 335 2144 1174
Fragments arranged by size:
2144 1174 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 3734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1789 2281 2579 910
Size: 879 492 298 2065
Fragments arranged by size:
2065 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3438 3438
Size: 3734
BsaAI yAC'GTr
Cuts at: 1087 1087
Size: 3734
BsaBI GATnn'nnATC
Cuts at: 6 1248 3285 6
Size: 1242 2037 455
Fragments arranged by size:
2037 1242 455
BsaHI Gr'CG_yC
Cuts at: 192 290 1165 1413 2115 3611 192
Size: 98 875 248 702 1496 315
Fragments arranged by size:
1496 875 702 315 248 98
BsaWI w'CCGG_w
Cuts at: 923 1444 2122 2396 2543 2954 923
Size: 521 678 274 147 411 1703
Fragments arranged by size:
1703 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 379
Size: 3734
BseYI C'CCAG_C
Cuts at: 1159 1917 2494 2990 1159
Size: 758 577 496 1903
Fragments arranged by size:
1903 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 523 424
Size: 99 3635
BsiEI CG_ry'CG
Cuts at: 104 563 1276 1322 2530 104
Size: 459 713 46 1208 1308
Fragments arranged by size:
1308 1208 713 459 46
BsiHKAI G_wGCw'C
Cuts at: 124 488 680 748 1526 1716 2508 124
Size: 364 192 68 778 190 792 1350
Fragments arranged by size:
1350 792 778 364 192 190 68
Bsp1286I G_dGCh'C
Cuts at: 124 488 517 640 661 680 748 1359 1452
Size: 364 29 123 21 19 68 611 93
Cuts at: 1452 1526 1716 1778 2508 124
Size: 74 190 62 730 1350
Fragments arranged by size:
1350 730 611 364 190 123 93 74
68 62 29 21 19
BspEI T'CCGG_A
Cuts at: 2954 2954
Size: 3734
BspHI T'CATG_A
Cuts at: 2910 2910
Size: 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 476 545 784 998 1300 1681 476
Size: 69 239 214 302 381 2529
Fragments arranged by size:
2529 381 302 239 214 69
BsrBI CCG'CTC
Cuts at: 50 2026 2080 3564 3729 50
Size: 1976 54 1484 165 55
Fragments arranged by size:
1976 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1646 1646
Size: 3734
BsrFI r'CCGG_y
Cuts at: 558 1732 1913 2122 558
Size: 1174 181 209 2170
Fragments arranged by size:
2170 1174 209 181
BssSI C'ACGA_G
Cuts at: 2005 2363 2005
Size: 358 3376
BstAPI GCAn_nnn'nTGC
Cuts at: 1111 1111
Size: 3734
BstBI TT'CG_AA
Cuts at: 2095 2095
Size: 3734
BstXI CCAn_nnnn'nTGG
Cuts at: 654 654
Size: 3734
BstYI r'GATC_y
Cuts at: 1 164 314 759 1226 1249 1584 1830 2842
Size: 163 150 445 467 23 335 246 1012
Cuts at: 2842 2928 2940 3286 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 445 346 335 246 163
150 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 140 140
Size: 3734
BtgI C'CryG_G
Cuts at: 632 653 632
Size: 21 3713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1684 1865 2028 1684
Size: 181 163 3390
Fragments arranged by size:
3390 181 163
BtsI GCAGTG_nn'
Cuts at: 681 977 681
Size: 296 3438
DraI TTT'AAA
Cuts at: 733 733
Size: 3734
DraIII CAC_nnn'GTG
Cuts at: 633 633
Size: 3734
DrdI GACnn_nn'nnGTC
Cuts at: 1440 2298 1440
Size: 858 2876
EaeI y'GGCC_r
Cuts at: 479 560 1319 1493 1884 1911 3256 479
Size: 81 759 174 391 27 1345 957
Fragments arranged by size:
1345 957 759 391 174 81 27
EagI C'GGCC_G
Cuts at: 560 1319 560
Size: 759 2975
EarI CTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1942 2252 2398 3544 1942
Size: 310 146 1146 2132
Fragments arranged by size:
2132 1146 310 146
EcoICRI GAG'CTC
Cuts at: 678 746 678
Size: 68 3666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1559 1991 2738 3006 1559
Size: 432 747 268 2287
Fragments arranged by size:
2287 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 144 513 514 637 873 144
Size: 369 1 123 236 3005
Fragments arranged by size:
3005 369 236 123 1
EcoRI G'AATT_C
Cuts at: 738 738
Size: 3734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1753 1753
Size: 3734
FspI TGC'GCA
Cuts at: 712 1515 712
Size: 803 2931
HaeII r_GCGC'y
Cuts at: 195 251 534 1168 1416 2438 3417 195
Size: 56 283 634 248 1022 979 512
Fragments arranged by size:
1022 979 634 512 283 248 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 321 353 1576 1606 1608 1638 321
Size: 32 1223 30 2 30 2417
Fragments arranged by size:
2417 1223 32 30 30 2
HincII GTy'rAC
Cuts at: 773 773
Size: 3734
KasI G'GCGC_C
Cuts at: 191 1164 1412 191
Size: 973 248 2513
Fragments arranged by size:
2513 973 248
KpnI G_GTAC'C
Cuts at: 604 754 604
Size: 150 3584
MluI A'CGCG_T
Cuts at: 3167 3167
Size: 3734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2405 2589 2405
Size: 184 3550
MscI TGG'CCA
Cuts at: 1495 1495
Size: 3734
MslI CAynn'nnrTG
Cuts at: 470 652 670 1723 1850 470
Size: 182 18 1053 127 2354
Fragments arranged by size:
2354 1053 182 127 18
MspA1I CmG'CkG
Cuts at: 189 722 845 1159 1519 2532 2777 189
Size: 533 123 314 360 1013 245 1146
Fragments arranged by size:
1146 1013 533 360 314 245 123
NaeI GCC'GGC
Cuts at: 560 1915 560
Size: 1355 2379
NarI GG'CG_CC
Cuts at: 192 1165 1413 192
Size: 973 248 2513
Fragments arranged by size:
2513 973 248
NgoMIV G'CCGG_C
Cuts at: 558 1913 558
Size: 1355 2379
NspI r_CATG'y
Cuts at: 475 787 1287 2194 475
Size: 312 500 907 2015
Fragments arranged by size:
2015 907 500 312
PasI CC'CwG_GG
Cuts at: 351 351
Size: 3734
PciI A'CATG_T
Cuts at: 2190 2190
Size: 3734
PflMI CCAn_nnn'nTGG
Cuts at: 633 633
Size: 3734
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 3702
PspOMI G'GGCC_C
Cuts at: 513 636 513
Size: 123 3611
PstI C_TGCA'G
Cuts at: 781 1466 781
Size: 685 3049
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3734
PvuII CAG'CTG
Cuts at: 1159 1519 1159
Size: 360 3374
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3734
SacI G_AGCT'C
Cuts at: 680 748 680
Size: 68 3666
SalI G'TCGA_C
Cuts at: 771 771
Size: 3734
SapI GCTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3734
ScaI AGT'ACT
Cuts at: 3251 3251
Size: 3734
SfcI C'TryA_G
Cuts at: 33 777 1462 2455 2646 33
Size: 744 685 993 191 1121
Fragments arranged by size:
1121 993 744 685 191
SfoI GGC'GCC
Cuts at: 193 1166 1414 193
Size: 973 248 2513
Fragments arranged by size:
2513 973 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
SmaI CCC'GGG
Cuts at: 756 756
Size: 3734
SmlI C'TyrA_G
Cuts at: 889 1804 2296 2558 889
Size: 915 492 262 2065
Fragments arranged by size:
2065 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3734
SphI G_CATG'C
Cuts at: 787 1287 787
Size: 500 3234
StyI C'CwwG_G
Cuts at: 868 1222 868
Size: 354 3380
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 139 1317 1450 1909 2118 139
Size: 1178 133 459 209 1755
Fragments arranged by size:
1755 1178 459 209 133
TatI w'GTAC_w
Cuts at: 3249 3249
Size: 3734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 149 181 3251 3283 149
Size: 32 3070 32 600
Fragments arranged by size:
3070 600 32 32
Tth111I GACn'n_nGTC
Cuts at: 241 1531 241
Size: 1290 2444
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1229 1229
Size: 3734
XmaI C'CCGG_G
Cuts at: 754 754
Size: 3734
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BpmI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI