Restriction map for
pF1KB6930
(Circular) (Six-base) MAPSORT of: pF1KB6930.seq Check: 4664 from: 1 to: 3797
pF1KB6930 3797 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 835 835
Size: 3797
Acc65I G'GTAC_C
Cuts at: 813 813
Size: 3797
AclI AA'CG_TT
Cuts at: 3637 3637
Size: 3797
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1622 2054 2801 3069 655
Size: 967 432 747 268 1383
Fragments arranged by size:
1383 967 747 432 268
AflIII A'CryG_T
Cuts at: 1149 2253 3230 1149
Size: 1104 977 1716
Fragments arranged by size:
1716 1104 977
AgeI A'CCGG_T
Cuts at: 2185 2185
Size: 3797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 148 148
Size: 3797
AlwNI CAG_nnn'CTG
Cuts at: 1102 2669 1102
Size: 1567 2230
ApaLI G'TGCA_C
Cuts at: 2567 2567
Size: 3797
ApoI r'AATT_y
Cuts at: 466 656 801 3150 3406 466
Size: 190 145 2349 256 857
Fragments arranged by size:
2349 857 256 190 145
AseI AT'TA_AT
Cuts at: 20 20
Size: 3797
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
AvaI C'yCGr_G
Cuts at: 817 817
Size: 3797
BamHI G'GATC_C
Cuts at: 822 822
Size: 3797
BanI G'GyrC_C
Cuts at: 241 813 1227 1475 1510 241
Size: 572 414 248 35 2528
Fragments arranged by size:
2528 572 414 248 35
BanII G_rGCy'C
Cuts at: 811 1841 811
Size: 1030 2767
BbeI G_GCGC'C
Cuts at: 245 1231 1479 245
Size: 986 248 2563
Fragments arranged by size:
2563 986 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3763
BciVI GTATCCnnnnn_n'
Cuts at: 1695 2456 3797 1695
Size: 761 1341 1695
Fragments arranged by size:
1695 1341 761
BclI T'GATC_A
Cuts at: 1317 1317
Size: 3797
BglII A'GATC_T
Cuts at: 1312 3349 1312
Size: 2037 1760
BlpI GC'TnA_GC
Cuts at: 112 909 112
Size: 797 3000
Bme1580I G_kGCm'C
Cuts at: 1422 1515 2571 1422
Size: 93 1056 2648
Fragments arranged by size:
2648 1056 93
BmgBI CAC'GTC
Cuts at: 141 199 141
Size: 58 3739
BmrI ACTGGGnnnn_n'
Cuts at: 252 1009 1090 1425 3569 252
Size: 757 81 335 2144 480
Fragments arranged by size:
2144 757 480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 3797
Bpu10I CC'TnA_GC
Cuts at: 151 151
Size: 3797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 973 1852 2344 2642 619
Size: 354 879 492 298 1774
Fragments arranged by size:
1774 879 492 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 3501 3501
Size: 3797
BsaAI yAC'GTr
Cuts at: 1150 1150
Size: 3797
BsaBI GATnn'nnATC
Cuts at: 6 406 1311 3348 6
Size: 400 905 2037 455
Fragments arranged by size:
2037 905 455 400
BsaHI Gr'CG_yC
Cuts at: 242 1228 1476 2178 3674 242
Size: 986 248 702 1496 365
Fragments arranged by size:
1496 986 702 365 248
BsaWI w'CCGG_w
Cuts at: 986 1507 2185 2459 2606 3017 986
Size: 521 678 274 147 411 1766
Fragments arranged by size:
1766 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 3797
BseYI C'CCAG_C
Cuts at: 1222 1980 2557 3053 1222
Size: 758 577 496 1966
Fragments arranged by size:
1966 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 790
Size: 3797
BsiEI CG_ry'CG
Cuts at: 104 178 1339 1385 2593 104
Size: 74 1161 46 1208 1308
Fragments arranged by size:
1308 1208 1161 74 46
BsiHKAI G_wGCw'C
Cuts at: 811 1589 1779 2571 811
Size: 778 190 792 2037
Fragments arranged by size:
2037 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 205 205
Size: 3797
Bsp1286I G_dGCh'C
Cuts at: 811 1422 1515 1589 1779 1841 2571 811
Size: 611 93 74 190 62 730 2037
Fragments arranged by size:
2037 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3017 3017
Size: 3797
BspHI T'CATG_A
Cuts at: 203 2973 203
Size: 2770 1027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 847 1061 1363 1744 847
Size: 214 302 381 2900
Fragments arranged by size:
2900 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2089 2143 3627 3792 50
Size: 2039 54 1484 165 55
Fragments arranged by size:
2039 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1709 1709
Size: 3797
BsrFI r'CCGG_y
Cuts at: 128 173 244 1795 1976 2185 128
Size: 45 71 1551 181 209 1740
Fragments arranged by size:
1740 1551 209 181 71 45
BssSI C'ACGA_G
Cuts at: 2068 2426 2068
Size: 358 3439
BstAPI GCAn_nnn'nTGC
Cuts at: 1174 1174
Size: 3797
BstBI TT'CG_AA
Cuts at: 2158 2158
Size: 3797
BstYI r'GATC_y
Cuts at: 1 822 1289 1312 1647 1893 2905 2991 3003
Size: 821 467 23 335 246 1012 86 12
Cuts at: 3003 3349 1
Size: 346 449
Fragments arranged by size:
1012 821 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 524 524
Size: 3797
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1747 1928 2091 1747
Size: 181 163 3453
Fragments arranged by size:
3453 181 163
BtsI GCAGTG_nn'
Cuts at: 1040 1040
Size: 3797
DraI TTT'AAA
Cuts at: 796 796
Size: 3797
DrdI GACnn_nn'nnGTC
Cuts at: 1503 2361 1503
Size: 858 2939
EaeI y'GGCC_r
Cuts at: 175 1382 1556 1947 1974 3319 175
Size: 1207 174 391 27 1345 653
Fragments arranged by size:
1345 1207 653 391 174 27
EagI C'GGCC_G
Cuts at: 175 1382 175
Size: 1207 2590
EarI CTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2005 2315 2461 3607 2005
Size: 310 146 1146 2195
Fragments arranged by size:
2195 1146 310 146
EcoICRI GAG'CTC
Cuts at: 809 809
Size: 3797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1622 2054 2801 3069 655
Size: 967 432 747 268 1383
Fragments arranged by size:
1383 967 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 254 936 254
Size: 682 3115
EcoRI G'AATT_C
Cuts at: 656 801 656
Size: 145 3652
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 520 1816 520
Size: 1296 2501
FspI TGC'GCA
Cuts at: 1578 1578
Size: 3797
HaeII r_GCGC'y
Cuts at: 245 1231 1479 2501 3480 245
Size: 986 248 1022 979 562
Fragments arranged by size:
1022 986 979 562 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1639 1669 1671 1701 1639
Size: 30 2 30 3735
Fragments arranged by size:
3735 30 30 2
HincII GTy'rAC
Cuts at: 677 836 677
Size: 159 3638
KasI G'GCGC_C
Cuts at: 241 1227 1475 241
Size: 986 248 2563
Fragments arranged by size:
2563 986 248
KpnI G_GTAC'C
Cuts at: 817 817
Size: 3797
MluI A'CGCG_T
Cuts at: 3230 3230
Size: 3797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2468 2652 2468
Size: 184 3613
MscI TGG'CCA
Cuts at: 1558 1558
Size: 3797
MslI CAynn'nnrTG
Cuts at: 1786 1913 1786
Size: 127 3670
MspA1I CmG'CkG
Cuts at: 119 213 908 1222 1582 2595 2840 119
Size: 94 695 314 360 1013 245 1076
Fragments arranged by size:
1076 1013 695 360 314 245 94
NaeI GCC'GGC
Cuts at: 130 175 246 1978 130
Size: 45 71 1732 1949
Fragments arranged by size:
1949 1732 71 45
NarI GG'CG_CC
Cuts at: 242 1228 1476 242
Size: 986 248 2563
Fragments arranged by size:
2563 986 248
NcoI C'CATG_G
Cuts at: 524 524
Size: 3797
NgoMIV G'CCGG_C
Cuts at: 128 173 244 1976 128
Size: 45 71 1732 1949
Fragments arranged by size:
1949 1732 71 45
NspI r_CATG'y
Cuts at: 850 1350 2257 850
Size: 500 907 2390
Fragments arranged by size:
2390 907 500
PciI A'CATG_T
Cuts at: 2253 2253
Size: 3797
PflMI CCAn_nnn'nTGG
Cuts at: 144 144
Size: 3797
PmeI GTTT'AAAC
Cuts at: 796 796
Size: 3797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2936 2968 2936
Size: 32 3765
PpuMI rG'GwC_Cy
Cuts at: 254 254
Size: 3797
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 536 568 536
Size: 32 3765
PstI C_TGCA'G
Cuts at: 167 306 844 1529 167
Size: 139 538 685 2435
Fragments arranged by size:
2435 685 538 139
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3797
PvuII CAG'CTG
Cuts at: 1222 1582 1222
Size: 360 3437
RsrII CG'GwC_CG
Cuts at: 1992 1992
Size: 3797
SacI G_AGCT'C
Cuts at: 811 811
Size: 3797
SalI G'TCGA_C
Cuts at: 834 834
Size: 3797
SanDI GG'GwC_CC
Cuts at: 254 254
Size: 3797
SapI GCTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
SbfI CC_TGCA'GG
Cuts at: 844 844
Size: 3797
ScaI AGT'ACT
Cuts at: 3314 3314
Size: 3797
SfcI C'TryA_G
Cuts at: 33 163 302 840 1525 2518 2709 33
Size: 130 139 538 685 993 191 1121
Fragments arranged by size:
1121 993 685 538 191 139 130
SfoI GGC'GCC
Cuts at: 243 1229 1477 243
Size: 986 248 2563
Fragments arranged by size:
2563 986 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
SmaI CCC'GGG
Cuts at: 819 819
Size: 3797
SmlI C'TyrA_G
Cuts at: 598 952 1867 2359 2621 598
Size: 354 915 492 262 1774
Fragments arranged by size:
1774 915 492 354 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3797
SphI G_CATG'C
Cuts at: 850 1350 850
Size: 500 3297
StyI C'CwwG_G
Cuts at: 524 931 1285 524
Size: 407 354 3036
Fragments arranged by size:
3036 407 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1380 1513 1972 2181 1380
Size: 133 459 209 2996
Fragments arranged by size:
2996 459 209 133
TatI w'GTAC_w
Cuts at: 368 3312 368
Size: 2944 853
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 3765
Tth111I GACn'n_nGTC
Cuts at: 1594 1594
Size: 3797
XbaI T'CTAG_A
Cuts at: 828 828
Size: 3797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1292 1292
Size: 3797
XmaI C'CCGG_G
Cuts at: 817 817
Size: 3797
XmnI GAAnn'nnTTC
Cuts at: 466 466
Size: 3797
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmtI
BpmI BsaXI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI