Restriction map for
pF1KB6933
(Circular) (Six-base) MAPSORT of: pF1KB6933.seq Check: 459 from: 1 to: 3734
pF1KB6933 3734 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 772 772
Size: 3734
Acc65I G'GTAC_C
Cuts at: 750 750
Size: 3734
AclI AA'CG_TT
Cuts at: 3574 3574
Size: 3734
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 1559 1991 2738 3006 224
Size: 1335 432 747 268 952
Fragments arranged by size:
1335 952 747 432 268
AflIII A'CryG_T
Cuts at: 1086 2190 3167 1086
Size: 1104 977 1653
Fragments arranged by size:
1653 1104 977
AgeI A'CCGG_T
Cuts at: 2122 2122
Size: 3734
AlwNI CAG_nnn'CTG
Cuts at: 202 1039 2606 202
Size: 837 1567 1330
Fragments arranged by size:
1567 1330 837
ApaLI G'TGCA_C
Cuts at: 2504 2504
Size: 3734
ApoI r'AATT_y
Cuts at: 302 738 3087 3343 302
Size: 436 2349 256 693
Fragments arranged by size:
2349 693 436 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
AvaI C'yCGr_G
Cuts at: 754 754
Size: 3734
BamHI G'GATC_C
Cuts at: 636 759 636
Size: 123 3611
BanI G'GyrC_C
Cuts at: 750 1164 1412 1447 750
Size: 414 248 35 3037
Fragments arranged by size:
3037 414 248 35
BanII G_rGCy'C
Cuts at: 145 748 1778 145
Size: 603 1030 2101
Fragments arranged by size:
2101 1030 603
BbeI G_GCGC'C
Cuts at: 1168 1416 1168
Size: 248 3486
BciVI GTATCCnnnnn_n'
Cuts at: 1632 2393 3734 1632
Size: 761 1341 1632
Fragments arranged by size:
1632 1341 761
BclI T'GATC_A
Cuts at: 1254 1254
Size: 3734
BglII A'GATC_T
Cuts at: 1249 3286 1249
Size: 2037 1697
BlpI GC'TnA_GC
Cuts at: 846 846
Size: 3734
Bme1580I G_kGCm'C
Cuts at: 1359 1452 2508 1359
Size: 93 1056 2585
Fragments arranged by size:
2585 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 946 1027 1362 3506 946
Size: 81 335 2144 1174
Fragments arranged by size:
2144 1174 335 81
BmtI G_CTAG'C
Cuts at: 319 319
Size: 3734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 3734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 585 179
Size: 406 3328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1789 2281 2579 910
Size: 879 492 298 2065
Fragments arranged by size:
2065 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 176 3438 176
Size: 3262 472
BsaAI yAC'GTr
Cuts at: 1087 1087
Size: 3734
BsaBI GATnn'nnATC
Cuts at: 6 1248 3285 6
Size: 1242 2037 455
Fragments arranged by size:
2037 1242 455
BsaHI Gr'CG_yC
Cuts at: 1165 1413 2115 3611 1165
Size: 248 702 1496 1288
Fragments arranged by size:
1496 1288 702 248
BsaWI w'CCGG_w
Cuts at: 923 1444 2122 2396 2543 2954 923
Size: 521 678 274 147 411 1703
Fragments arranged by size:
1703 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 329 329
Size: 3734
BseYI C'CCAG_C
Cuts at: 145 186 236 355 1159 1917 2494 2990 145
Size: 41 50 119 804 758 577 496 889
Fragments arranged by size:
889 804 758 577 496 119 50 41
BsiEI CG_ry'CG
Cuts at: 104 1276 1322 2530 104
Size: 1172 46 1208 1308
Fragments arranged by size:
1308 1208 1172 46
BsiHKAI G_wGCw'C
Cuts at: 748 1526 1716 2508 748
Size: 778 190 792 1974
Fragments arranged by size:
1974 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 145 748 1359 1452 1526 1716 1778 2508 145
Size: 603 611 93 74 190 62 730 1371
Fragments arranged by size:
1371 730 611 603 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2954 2954
Size: 3734
BspHI T'CATG_A
Cuts at: 2910 2910
Size: 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 784 998 1300 1681 784
Size: 214 302 381 2837
Fragments arranged by size:
2837 381 302 214
BsrBI CCG'CTC
Cuts at: 50 193 2026 2080 3564 3729 50
Size: 143 1833 54 1484 165 55
Fragments arranged by size:
1833 1484 165 143 55 54
BsrDI GCAATG_nn'
Cuts at: 1646 1646
Size: 3734
BsrFI r'CCGG_y
Cuts at: 1732 1913 2122 1732
Size: 181 209 3344
Fragments arranged by size:
3344 209 181
BssSI C'ACGA_G
Cuts at: 2005 2363 2005
Size: 358 3376
BstAPI GCAn_nnn'nTGC
Cuts at: 1111 1111
Size: 3734
BstBI TT'CG_AA
Cuts at: 2095 2095
Size: 3734
BstYI r'GATC_y
Cuts at: 1 636 759 1226 1249 1584 1830 2842 2928
Size: 635 123 467 23 335 246 1012 86
Cuts at: 2928 2940 3286 1
Size: 12 346 449
Fragments arranged by size:
1012 635 467 449 346 335 246 123
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1684 1865 2028 1684
Size: 181 163 3390
Fragments arranged by size:
3390 181 163
BtsI GCAGTG_nn'
Cuts at: 162 272 357 510 977 162
Size: 110 85 153 467 2919
Fragments arranged by size:
2919 467 153 110 85
DraI TTT'AAA
Cuts at: 733 733
Size: 3734
DrdI GACnn_nn'nnGTC
Cuts at: 1440 2298 1440
Size: 858 2876
EaeI y'GGCC_r
Cuts at: 1319 1493 1884 1911 3256 1319
Size: 174 391 27 1345 1797
Fragments arranged by size:
1797 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1319 1319
Size: 3734
EarI CTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1942 2252 2398 3544 1942
Size: 310 146 1146 2132
Fragments arranged by size:
2132 1146 310 146
EcoICRI GAG'CTC
Cuts at: 746 746
Size: 3734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 224 585 1559 1991 2738 3006 179
Size: 45 361 974 432 747 268 907
Fragments arranged by size:
974 907 747 432 361 268 45
EcoO109I rG'GnC_Cy
Cuts at: 873 873
Size: 3734
EcoRI G'AATT_C
Cuts at: 302 738 302
Size: 436 3298
EcoRV GAT'ATC
Cuts at: 447 447
Size: 3734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 242 1753 242
Size: 1511 2223
FspI TGC'GCA
Cuts at: 1515 1515
Size: 3734
HaeII r_GCGC'y
Cuts at: 1168 1416 2438 3417 1168
Size: 248 1022 979 1485
Fragments arranged by size:
1485 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1576 1606 1608 1638 1576
Size: 30 2 30 3672
Fragments arranged by size:
3672 30 30 2
HincII GTy'rAC
Cuts at: 773 773
Size: 3734
KasI G'GCGC_C
Cuts at: 1164 1412 1164
Size: 248 3486
KpnI G_GTAC'C
Cuts at: 754 754
Size: 3734
MluI A'CGCG_T
Cuts at: 3167 3167
Size: 3734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 627 2405 2589 627
Size: 1778 184 1772
Fragments arranged by size:
1778 1772 184
MscI TGG'CCA
Cuts at: 1495 1495
Size: 3734
MslI CAynn'nnrTG
Cuts at: 1723 1850 1723
Size: 127 3607
MspA1I CmG'CkG
Cuts at: 845 1159 1519 2532 2777 845
Size: 314 360 1013 245 1802
Fragments arranged by size:
1802 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1915 1915
Size: 3734
NarI GG'CG_CC
Cuts at: 1165 1413 1165
Size: 248 3486
NgoMIV G'CCGG_C
Cuts at: 1913 1913
Size: 3734
NheI G'CTAG_C
Cuts at: 315 315
Size: 3734
NspI r_CATG'y
Cuts at: 787 1287 2194 787
Size: 500 907 2327
Fragments arranged by size:
2327 907 500
PciI A'CATG_T
Cuts at: 2190 2190
Size: 3734
PflMI CCAn_nnn'nTGG
Cuts at: 707 707
Size: 3734
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 3702
PstI C_TGCA'G
Cuts at: 781 1466 781
Size: 685 3049
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3734
PvuII CAG'CTG
Cuts at: 1159 1519 1159
Size: 360 3374
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3734
SacI G_AGCT'C
Cuts at: 748 748
Size: 3734
SalI G'TCGA_C
Cuts at: 771 771
Size: 3734
SapI GCTCTTCn'nnn_
Cuts at: 1757 1967 1757
Size: 210 3524
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3734
ScaI AGT'ACT
Cuts at: 3251 3251
Size: 3734
SfcI C'TryA_G
Cuts at: 33 777 1462 2455 2646 33
Size: 744 685 993 191 1121
Fragments arranged by size:
1121 993 744 685 191
SfoI GGC'GCC
Cuts at: 1166 1414 1166
Size: 248 3486
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
SmaI CCC'GGG
Cuts at: 756 756
Size: 3734
SmlI C'TyrA_G
Cuts at: 889 1804 2296 2558 889
Size: 915 492 262 2065
Fragments arranged by size:
2065 915 492 262
SpeI A'CTAG_T
Cuts at: 67 289 67
Size: 222 3512
SphI G_CATG'C
Cuts at: 787 1287 787
Size: 500 3234
StyI C'CwwG_G
Cuts at: 868 1222 868
Size: 354 3380
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 250 1317 1450 1909 2118 250
Size: 1067 133 459 209 1866
Fragments arranged by size:
1866 1067 459 209 133
TatI w'GTAC_w
Cuts at: 3249 3249
Size: 3734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 3702
Tth111I GACn'n_nGTC
Cuts at: 1531 1531
Size: 3734
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1229 1229
Size: 3734
XmaI C'CCGG_G
Cuts at: 754 754
Size: 3734
XmnI GAAnn'nnTTC
Cuts at: 225 225
Size: 3734
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI