Restriction map for
pF1KB6934
(Circular) (Six-base) MAPSORT of: pF1KB6934.seq Check: 5152 from: 1 to: 3770
pF1KB6934 3770 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 546 808 546
Size: 262 3508
Acc65I G'GTAC_C
Cuts at: 786 786
Size: 3770
AclI AA'CG_TT
Cuts at: 594 3610 594
Size: 3016 754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 564 685 1595 2027 2774 3042 564
Size: 121 910 432 747 268 1292
Fragments arranged by size:
1292 910 747 432 268 121
AflIII A'CryG_T
Cuts at: 282 1122 2226 3203 282
Size: 840 1104 977 849
Fragments arranged by size:
1104 977 849 840
AgeI A'CCGG_T
Cuts at: 2158 2158
Size: 3770
AlwNI CAG_nnn'CTG
Cuts at: 183 212 1075 2642 183
Size: 29 863 1567 1311
Fragments arranged by size:
1567 1311 863 29
ApaLI G'TGCA_C
Cuts at: 2540 2540
Size: 3770
ApoI r'AATT_y
Cuts at: 658 774 3123 3379 658
Size: 116 2349 256 1049
Fragments arranged by size:
2349 1049 256 116
AseI AT'TA_AT
Cuts at: 20 20
Size: 3770
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
AvaI C'yCGr_G
Cuts at: 790 790
Size: 3770
BamHI G'GATC_C
Cuts at: 795 795
Size: 3770
BanI G'GyrC_C
Cuts at: 786 1200 1448 1483 786
Size: 414 248 35 3073
Fragments arranged by size:
3073 414 248 35
BanII G_rGCy'C
Cuts at: 784 1814 784
Size: 1030 2740
BbeI G_GCGC'C
Cuts at: 1204 1452 1204
Size: 248 3522
BciVI GTATCCnnnnn_n'
Cuts at: 1668 2429 3770 1668
Size: 761 1341 1668
Fragments arranged by size:
1668 1341 761
BclI T'GATC_A
Cuts at: 1290 1290
Size: 3770
BglII A'GATC_T
Cuts at: 1285 3322 1285
Size: 2037 1733
BlpI GC'TnA_GC
Cuts at: 882 882
Size: 3770
Bme1580I G_kGCm'C
Cuts at: 1395 1488 2544 1395
Size: 93 1056 2621
Fragments arranged by size:
2621 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 534 982 1063 1398 3542 534
Size: 448 81 335 2144 762
Fragments arranged by size:
2144 762 448 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 3770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1825 2317 2615 946
Size: 879 492 298 2101
Fragments arranged by size:
2101 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3474 3474
Size: 3770
BsaAI yAC'GTr
Cuts at: 285 1123 285
Size: 838 2932
BsaBI GATnn'nnATC
Cuts at: 6 1284 3321 6
Size: 1278 2037 455
Fragments arranged by size:
2037 1278 455
BsaHI Gr'CG_yC
Cuts at: 1201 1449 2151 3647 1201
Size: 248 702 1496 1324
Fragments arranged by size:
1496 1324 702 248
BsaWI w'CCGG_w
Cuts at: 959 1480 2158 2432 2579 2990 959
Size: 521 678 274 147 411 1739
Fragments arranged by size:
1739 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1195 1953 2530 3026 1195
Size: 758 577 496 1939
Fragments arranged by size:
1939 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 127
Size: 3770
BsiEI CG_ry'CG
Cuts at: 104 197 1312 1358 2566 104
Size: 93 1115 46 1208 1308
Fragments arranged by size:
1308 1208 1115 93 46
BsiHKAI G_wGCw'C
Cuts at: 745 784 1562 1752 2544 745
Size: 39 778 190 792 1971
Fragments arranged by size:
1971 792 778 190 39
Bsp1286I G_dGCh'C
Cuts at: 745 784 1395 1488 1562 1752 1814 2544 745
Size: 39 611 93 74 190 62 730 1971
Fragments arranged by size:
1971 730 611 190 93 74 62 39
BspEI T'CCGG_A
Cuts at: 2990 2990
Size: 3770
BspHI T'CATG_A
Cuts at: 2946 2946
Size: 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 820 1034 1336 1717 820
Size: 214 302 381 2873
Fragments arranged by size:
2873 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2062 2116 3600 3765 50
Size: 2012 54 1484 165 55
Fragments arranged by size:
2012 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 246 252 264 1682 246
Size: 6 12 1418 2334
Fragments arranged by size:
2334 1418 12 6
BsrFI r'CCGG_y
Cuts at: 192 196 1768 1949 2158 192
Size: 4 1572 181 209 1804
Fragments arranged by size:
1804 1572 209 181 4
BssSI C'ACGA_G
Cuts at: 2041 2399 2041
Size: 358 3412
BstAPI GCAn_nnn'nTGC
Cuts at: 183 1147 183
Size: 964 2806
BstBI TT'CG_AA
Cuts at: 2131 2131
Size: 3770
BstYI r'GATC_y
Cuts at: 1 795 1262 1285 1620 1866 2878 2964 2976
Size: 794 467 23 335 246 1012 86 12
Cuts at: 2976 3322 1
Size: 346 449
Fragments arranged by size:
1012 794 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1720 1901 2064 1720
Size: 181 163 3426
Fragments arranged by size:
3426 181 163
BtsI GCAGTG_nn'
Cuts at: 1013 1013
Size: 3770
DraI TTT'AAA
Cuts at: 769 769
Size: 3770
DrdI GACnn_nn'nnGTC
Cuts at: 1476 2334 1476
Size: 858 2912
EaeI y'GGCC_r
Cuts at: 194 1355 1529 1920 1947 3292 194
Size: 1161 174 391 27 1345 672
Fragments arranged by size:
1345 1161 672 391 174 27
EagI C'GGCC_G
Cuts at: 194 1355 194
Size: 1161 2609
EarI CTCTTCn'nnn_
Cuts at: 691 1793 2003 691
Size: 1102 210 2458
Fragments arranged by size:
2458 1102 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1978 2288 2434 3580 1978
Size: 310 146 1146 2168
Fragments arranged by size:
2168 1146 310 146
EcoICRI GAG'CTC
Cuts at: 782 782
Size: 3770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 564 685 1595 2027 2774 3042 564
Size: 121 910 432 747 268 1292
Fragments arranged by size:
1292 910 747 432 268 121
EcoO109I rG'GnC_Cy
Cuts at: 909 909
Size: 3770
EcoRI G'AATT_C
Cuts at: 774 774
Size: 3770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1789 1789
Size: 3770
FseI GG_CCGG'CC
Cuts at: 200 200
Size: 3770
FspI TGC'GCA
Cuts at: 1551 1551
Size: 3770
HaeII r_GCGC'y
Cuts at: 1204 1452 2474 3453 1204
Size: 248 1022 979 1521
Fragments arranged by size:
1521 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 442 474 514 546 1612 1642 1644 1674 442
Size: 32 40 32 1066 30 2 30 2538
Fragments arranged by size:
2538 1066 40 32 32 30 30 2
HincII GTy'rAC
Cuts at: 809 809
Size: 3770
KasI G'GCGC_C
Cuts at: 1200 1448 1200
Size: 248 3522
KpnI G_GTAC'C
Cuts at: 790 790
Size: 3770
MluI A'CGCG_T
Cuts at: 3203 3203
Size: 3770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2441 2625 2441
Size: 184 3586
MscI TGG'CCA
Cuts at: 1531 1531
Size: 3770
MslI CAynn'nnrTG
Cuts at: 1759 1886 1759
Size: 127 3643
MspA1I CmG'CkG
Cuts at: 881 1195 1555 2568 2813 881
Size: 314 360 1013 245 1838
Fragments arranged by size:
1838 1013 360 314 245
NaeI GCC'GGC
Cuts at: 194 198 1951 194
Size: 4 1753 2013
Fragments arranged by size:
2013 1753 4
NarI GG'CG_CC
Cuts at: 1201 1449 1201
Size: 248 3522
NgoMIV G'CCGG_C
Cuts at: 192 196 1949 192
Size: 4 1753 2013
Fragments arranged by size:
2013 1753 4
NspI r_CATG'y
Cuts at: 823 1323 2230 823
Size: 500 907 2363
Fragments arranged by size:
2363 907 500
PciI A'CATG_T
Cuts at: 2226 2226
Size: 3770
PmeI GTTT'AAAC
Cuts at: 769 769
Size: 3770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 3738
PstI C_TGCA'G
Cuts at: 817 1502 817
Size: 685 3085
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3770
PvuII CAG'CTG
Cuts at: 1195 1555 1195
Size: 360 3410
RsrII CG'GwC_CG
Cuts at: 1965 1965
Size: 3770
SacI G_AGCT'C
Cuts at: 784 784
Size: 3770
SalI G'TCGA_C
Cuts at: 807 807
Size: 3770
SapI GCTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
SbfI CC_TGCA'GG
Cuts at: 817 817
Size: 3770
ScaI AGT'ACT
Cuts at: 3287 3287
Size: 3770
SfcI C'TryA_G
Cuts at: 33 813 1498 2491 2682 33
Size: 780 685 993 191 1121
Fragments arranged by size:
1121 993 780 685 191
SfoI GGC'GCC
Cuts at: 1202 1450 1202
Size: 248 3522
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
SmaI CCC'GGG
Cuts at: 792 792
Size: 3770
SmlI C'TyrA_G
Cuts at: 925 1840 2332 2594 925
Size: 915 492 262 2101
Fragments arranged by size:
2101 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3770
SphI G_CATG'C
Cuts at: 823 1323 823
Size: 500 3270
StyI C'CwwG_G
Cuts at: 566 904 1258 566
Size: 338 354 3078
Fragments arranged by size:
3078 354 338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 204 579 1353 1486 1945 2154 204
Size: 375 774 133 459 209 1820
Fragments arranged by size:
1820 774 459 375 209 133
TatI w'GTAC_w
Cuts at: 3285 3285
Size: 3770
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 274 306 3287 3319 274
Size: 32 2981 32 725
Fragments arranged by size:
2981 725 32 32
Tth111I GACn'n_nGTC
Cuts at: 1567 1567
Size: 3770
XbaI T'CTAG_A
Cuts at: 801 801
Size: 3770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1265 1265
Size: 3770
XmaI C'CCGG_G
Cuts at: 790 790
Size: 3770
XmnI GAAnn'nnTTC
Cuts at: 557 670 557
Size: 113 3657
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI