Restriction map for
pF1KB6935
(Circular) (Six-base) MAPSORT of: pF1KB6935.seq Check: 459 from: 1 to: 3734
pF1KB6935 3734 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AatII G_ACGT'C
Cuts at: 223 223
Size: 3734
AccI GT'mk_AC
Cuts at: 772 772
Size: 3734
Acc65I G'GTAC_C
Cuts at: 750 750
Size: 3734
AclI AA'CG_TT
Cuts at: 728 3574 728
Size: 2846 888
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1559 1991 2738 3006 436
Size: 1123 432 747 268 1164
Fragments arranged by size:
1164 1123 747 432 268
AflIII A'CryG_T
Cuts at: 1086 2190 3167 1086
Size: 1104 977 1653
Fragments arranged by size:
1653 1104 977
AgeI A'CCGG_T
Cuts at: 629 2122 629
Size: 1493 2241
AlwNI CAG_nnn'CTG
Cuts at: 189 1039 2606 189
Size: 850 1567 1317
Fragments arranged by size:
1567 1317 850
ApaLI G'TGCA_C
Cuts at: 291 2504 291
Size: 2213 1521
ApoI r'AATT_y
Cuts at: 427 738 3087 3343 427
Size: 311 2349 256 818
Fragments arranged by size:
2349 818 311 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
AvaI C'yCGr_G
Cuts at: 325 702 754 325
Size: 377 52 3305
Fragments arranged by size:
3305 377 52
BamHI G'GATC_C
Cuts at: 759 759
Size: 3734
BanI G'GyrC_C
Cuts at: 750 1164 1412 1447 750
Size: 414 248 35 3037
Fragments arranged by size:
3037 414 248 35
BanII G_rGCy'C
Cuts at: 281 661 709 748 1778 281
Size: 380 48 39 1030 2237
Fragments arranged by size:
2237 1030 380 48 39
BbeI G_GCGC'C
Cuts at: 1168 1416 1168
Size: 248 3486
BciVI GTATCCnnnnn_n'
Cuts at: 1632 2393 3734 1632
Size: 761 1341 1632
Fragments arranged by size:
1632 1341 761
BclI T'GATC_A
Cuts at: 1254 1254
Size: 3734
BglII A'GATC_T
Cuts at: 1249 3286 1249
Size: 2037 1697
BlpI GC'TnA_GC
Cuts at: 846 846
Size: 3734
Bme1580I G_kGCm'C
Cuts at: 295 1359 1452 2508 295
Size: 1064 93 1056 1521
Fragments arranged by size:
1521 1064 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 946 1027 1362 3506 946
Size: 81 335 2144 1174
Fragments arranged by size:
2144 1174 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 3734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 184
Size: 3734
Bpu10I CC'TnA_GC
Cuts at: 660 660
Size: 3734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 910 1789 2281 2579 667
Size: 243 879 492 298 1822
Fragments arranged by size:
1822 879 492 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 3438 3438
Size: 3734
BsaAI yAC'GTr
Cuts at: 1087 1087
Size: 3734
BsaBI GATnn'nnATC
Cuts at: 6 1248 3285 6
Size: 1242 2037 455
Fragments arranged by size:
2037 1242 455
BsaHI Gr'CG_yC
Cuts at: 220 1165 1413 2115 3611 220
Size: 945 248 702 1496 343
Fragments arranged by size:
1496 945 702 343 248
BsaWI w'CCGG_w
Cuts at: 273 583 629 923 1444 2122 2396 2543 2954
Size: 310 46 294 521 678 274 147 411
Cuts at: 2954 273
Size: 1053
Fragments arranged by size:
1053 678 521 411 310 294 274 147
46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 648 678 648
Size: 30 3704
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 515 515
Size: 3734
BseYI C'CCAG_C
Cuts at: 246 1159 1917 2494 2990 246
Size: 913 758 577 496 990
Fragments arranged by size:
990 913 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 175
Size: 3734
BsiEI CG_ry'CG
Cuts at: 104 1276 1322 2530 104
Size: 1172 46 1208 1308
Fragments arranged by size:
1308 1208 1172 46
BsiHKAI G_wGCw'C
Cuts at: 295 661 748 1526 1716 2508 295
Size: 366 87 778 190 792 1521
Fragments arranged by size:
1521 792 778 366 190 87
BsmI GAATG_Cn'
Cuts at: 312 312
Size: 3734
Bsp1286I G_dGCh'C
Cuts at: 281 295 661 709 748 1359 1452 1526 1716
Size: 14 366 48 39 611 93 74 190
Cuts at: 1716 1778 2508 281
Size: 62 730 1507
Fragments arranged by size:
1507 730 611 366 190 93 74 62
48 39 14
BspEI T'CCGG_A
Cuts at: 273 583 2954 273
Size: 310 2371 1053
Fragments arranged by size:
2371 1053 310
BspHI T'CATG_A
Cuts at: 2910 2910
Size: 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 784 998 1300 1681 784
Size: 214 302 381 2837
Fragments arranged by size:
2837 381 302 214
BsrBI CCG'CTC
Cuts at: 50 691 2026 2080 3564 3729 50
Size: 641 1335 54 1484 165 55
Fragments arranged by size:
1484 1335 641 165 55 54
BsrDI GCAATG_nn'
Cuts at: 512 1646 512
Size: 1134 2600
BsrFI r'CCGG_y
Cuts at: 351 629 1732 1913 2122 351
Size: 278 1103 181 209 1963
Fragments arranged by size:
1963 1103 278 209 181
BssSI C'ACGA_G
Cuts at: 2005 2363 2005
Size: 358 3376
BstAPI GCAn_nnn'nTGC
Cuts at: 189 232 1111 189
Size: 43 879 2812
Fragments arranged by size:
2812 879 43
BstBI TT'CG_AA
Cuts at: 2095 2095
Size: 3734
BstYI r'GATC_y
Cuts at: 1 759 1226 1249 1584 1830 2842 2928 2940
Size: 758 467 23 335 246 1012 86 12
Cuts at: 2940 3286 1
Size: 346 449
Fragments arranged by size:
1012 758 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 236 569 107
Size: 129 333 3272
Fragments arranged by size:
3272 333 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1684 1865 2028 1684
Size: 181 163 3390
Fragments arranged by size:
3390 181 163
BtsI GCAGTG_nn'
Cuts at: 295 500 977 295
Size: 205 477 3052
Fragments arranged by size:
3052 477 205
DraI TTT'AAA
Cuts at: 733 733
Size: 3734
DraIII CAC_nnn'GTG
Cuts at: 549 549
Size: 3734
DrdI GACnn_nn'nnGTC
Cuts at: 1440 2298 1440
Size: 858 2876
EaeI y'GGCC_r
Cuts at: 593 720 1319 1493 1884 1911 3256 593
Size: 127 599 174 391 27 1345 1071
Fragments arranged by size:
1345 1071 599 391 174 127 27
EagI C'GGCC_G
Cuts at: 1319 1319
Size: 3734
EarI CTCTTCn'nnn_
Cuts at: 332 362 1757 1967 332
Size: 30 1395 210 2099
Fragments arranged by size:
2099 1395 210 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1942 2252 2398 3544 1942
Size: 310 146 1146 2132
Fragments arranged by size:
2132 1146 310 146
EcoICRI GAG'CTC
Cuts at: 659 746 659
Size: 87 3647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 436 1559 1991 2738 3006 184
Size: 252 1123 432 747 268 912
Fragments arranged by size:
1123 912 747 432 268 252
EcoNI CCTnn'n_nnAGG
Cuts at: 211 211
Size: 3734
EcoO109I rG'GnC_Cy
Cuts at: 243 674 873 243
Size: 431 199 3104
Fragments arranged by size:
3104 431 199
EcoRI G'AATT_C
Cuts at: 738 738
Size: 3734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 663 1753 663
Size: 1090 2644
FspI TGC'GCA
Cuts at: 1515 1515
Size: 3734
HaeII r_GCGC'y
Cuts at: 1168 1416 2438 3417 1168
Size: 248 1022 979 1485
Fragments arranged by size:
1485 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 501 533 648 680 1576 1606 1608 1638 501
Size: 32 115 32 896 30 2 30 2597
Fragments arranged by size:
2597 896 115 32 32 30 30 2
HincII GTy'rAC
Cuts at: 773 773
Size: 3734
KasI G'GCGC_C
Cuts at: 1164 1412 1164
Size: 248 3486
KpnI G_GTAC'C
Cuts at: 754 754
Size: 3734
MluI A'CGCG_T
Cuts at: 3167 3167
Size: 3734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2405 2589 2405
Size: 184 3550
MscI TGG'CCA
Cuts at: 1495 1495
Size: 3734
MslI CAynn'nnrTG
Cuts at: 1723 1850 1723
Size: 127 3607
MspA1I CmG'CkG
Cuts at: 229 256 563 845 1159 1519 2532 2777 229
Size: 27 307 282 314 360 1013 245 1186
Fragments arranged by size:
1186 1013 360 314 307 282 245 27
NaeI GCC'GGC
Cuts at: 353 1915 353
Size: 1562 2172
NarI GG'CG_CC
Cuts at: 1165 1413 1165
Size: 248 3486
NcoI C'CATG_G
Cuts at: 107 107
Size: 3734
NgoMIV G'CCGG_C
Cuts at: 351 1913 351
Size: 1562 2172
NspI r_CATG'y
Cuts at: 787 1287 2194 787
Size: 500 907 2327
Fragments arranged by size:
2327 907 500
PciI A'CATG_T
Cuts at: 2190 2190
Size: 3734
PflMI CCAn_nnn'nTGG
Cuts at: 549 633 549
Size: 84 3650
PmeI GTTT'AAAC
Cuts at: 733 733
Size: 3734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 3702
PpuMI rG'GwC_Cy
Cuts at: 243 243
Size: 3734
PstI C_TGCA'G
Cuts at: 228 559 781 1466 228
Size: 331 222 685 2496
Fragments arranged by size:
2496 685 331 222
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3734
PvuII CAG'CTG
Cuts at: 229 1159 1519 229
Size: 930 360 2444
Fragments arranged by size:
2444 930 360
RsrII CG'GwC_CG
Cuts at: 1929 1929
Size: 3734
SacI G_AGCT'C
Cuts at: 661 748 661
Size: 87 3647
SalI G'TCGA_C
Cuts at: 771 771
Size: 3734
SapI GCTCTTCn'nnn_
Cuts at: 362 1757 1967 362
Size: 1395 210 2129
Fragments arranged by size:
2129 1395 210
SbfI CC_TGCA'GG
Cuts at: 781 781
Size: 3734
ScaI AGT'ACT
Cuts at: 3251 3251
Size: 3734
SfcI C'TryA_G
Cuts at: 33 224 555 777 1462 2455 2646 33
Size: 191 331 222 685 993 191 1121
Fragments arranged by size:
1121 993 685 331 222 191 191
SfoI GGC'GCC
Cuts at: 1166 1414 1166
Size: 248 3486
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3734
SmaI CCC'GGG
Cuts at: 756 756
Size: 3734
SmlI C'TyrA_G
Cuts at: 682 889 1804 2296 2558 682
Size: 207 915 492 262 1858
Fragments arranged by size:
1858 915 492 262 207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3734
SphI G_CATG'C
Cuts at: 787 1287 787
Size: 500 3234
StyI C'CwwG_G
Cuts at: 107 868 1222 107
Size: 761 354 2619
Fragments arranged by size:
2619 761 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1317 1450 1909 2118 1317
Size: 133 459 209 2933
Fragments arranged by size:
2933 459 209 133
TatI w'GTAC_w
Cuts at: 3249 3249
Size: 3734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 581 613 3251 3283 581
Size: 32 2638 32 1032
Fragments arranged by size:
2638 1032 32 32
Tth111I GACn'n_nGTC
Cuts at: 1531 1531
Size: 3734
XbaI T'CTAG_A
Cuts at: 765 765
Size: 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1229 1229
Size: 3734
XmaI C'CCGG_G
Cuts at: 754 754
Size: 3734
XmnI GAAnn'nnTTC
Cuts at: 608 608
Size: 3734
ZraI GAC'GTC
Cuts at: 221 221
Size: 3734
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI