Restriction map for
pF1KB6939
(Circular) (Six-base) MAPSORT of: pF1KB6939.seq Check: 5290 from: 1 to: 3737
pF1KB6939 3737 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AatII G_ACGT'C
Cuts at: 529 529
Size: 3737
AccI GT'mk_AC
Cuts at: 775 775
Size: 3737
Acc65I G'GTAC_C
Cuts at: 753 753
Size: 3737
AclI AA'CG_TT
Cuts at: 3577 3577
Size: 3737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 1994 2741 3009 1562
Size: 432 747 268 2290
Fragments arranged by size:
2290 747 432 268
AfeI AGC'GCT
Cuts at: 208 571 208
Size: 363 3374
AflIII A'CryG_T
Cuts at: 479 1089 2193 3170 479
Size: 610 1104 977 1046
Fragments arranged by size:
1104 1046 977 610
AgeI A'CCGG_T
Cuts at: 676 2125 676
Size: 1449 2288
AlwNI CAG_nnn'CTG
Cuts at: 1042 2609 1042
Size: 1567 2170
ApaI G_GGCC'C
Cuts at: 437 437
Size: 3737
ApaLI G'TGCA_C
Cuts at: 2507 2507
Size: 3737
ApoI r'AATT_y
Cuts at: 741 3090 3346 741
Size: 2349 256 1132
Fragments arranged by size:
2349 1132 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3737
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
AvaI C'yCGr_G
Cuts at: 229 395 757 229
Size: 166 362 3209
Fragments arranged by size:
3209 362 166
BamHI G'GATC_C
Cuts at: 762 762
Size: 3737
BanI G'GyrC_C
Cuts at: 545 753 1167 1415 1450 545
Size: 208 414 248 35 2832
Fragments arranged by size:
2832 414 248 208 35
BanII G_rGCy'C
Cuts at: 166 328 437 476 751 1781 166
Size: 162 109 39 275 1030 2122
Fragments arranged by size:
2122 1030 275 162 109 39
BbeI G_GCGC'C
Cuts at: 1171 1419 1171
Size: 248 3489
BbsI GAAGACnn'nnnn_
Cuts at: 586 586
Size: 3737
BciVI GTATCCnnnnn_n'
Cuts at: 1635 2396 3737 1635
Size: 761 1341 1635
Fragments arranged by size:
1635 1341 761
BclI T'GATC_A
Cuts at: 1257 1257
Size: 3737
BglII A'GATC_T
Cuts at: 1252 3289 1252
Size: 2037 1700
BlpI GC'TnA_GC
Cuts at: 849 849
Size: 3737
Bme1580I G_kGCm'C
Cuts at: 437 487 602 1362 1455 2511 437
Size: 50 115 760 93 1056 1663
Fragments arranged by size:
1663 1056 760 115 93 50
BmrI ACTGGGnnnn_n'
Cuts at: 949 1030 1365 3509 949
Size: 81 335 2144 1177
Fragments arranged by size:
2144 1177 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 3737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 432
Size: 3737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 913 1792 2284 2582 181
Size: 732 879 492 298 1336
Fragments arranged by size:
1336 879 732 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3441 3441
Size: 3737
BsaAI yAC'GTr
Cuts at: 482 1090 482
Size: 608 3129
BsaBI GATnn'nnATC
Cuts at: 6 1251 3288 6
Size: 1245 2037 455
Fragments arranged by size:
2037 1245 455
BsaHI Gr'CG_yC
Cuts at: 226 526 1168 1416 2118 3614 226
Size: 300 642 248 702 1496 349
Fragments arranged by size:
1496 702 642 349 300 248
BsaWI w'CCGG_w
Cuts at: 676 926 1447 2125 2399 2546 2957 676
Size: 250 521 678 274 147 411 1456
Fragments arranged by size:
1456 678 521 411 274 250 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 418 418
Size: 3737
BseYI C'CCAG_C
Cuts at: 436 1162 1920 2497 2993 436
Size: 726 758 577 496 1180
Fragments arranged by size:
1180 758 726 577 496
BsiEI CG_ry'CG
Cuts at: 104 1279 1325 2533 104
Size: 1175 46 1208 1308
Fragments arranged by size:
1308 1208 1175 46
BsiHKAI G_wGCw'C
Cuts at: 166 611 751 1529 1719 2511 166
Size: 445 140 778 190 792 1392
Fragments arranged by size:
1392 792 778 445 190 140
BsmBI CGTCTCn'nnnn_
Cuts at: 533 533
Size: 3737
Bsp1286I G_dGCh'C
Cuts at: 166 328 437 476 487 602 611 751 1362
Size: 162 109 39 11 115 9 140 611
Cuts at: 1362 1455 1529 1719 1781 2511 166
Size: 93 74 190 62 730 1392
Fragments arranged by size:
1392 730 611 190 162 140 115 109
93 74 62 39 11 9
BspEI T'CCGG_A
Cuts at: 2957 2957
Size: 3737
BspHI T'CATG_A
Cuts at: 2913 2913
Size: 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 354 787 1001 1303 1684 354
Size: 433 214 302 381 2407
Fragments arranged by size:
2407 433 381 302 214
BsrBI CCG'CTC
Cuts at: 50 408 419 2029 2083 3567 3732 50
Size: 358 11 1610 54 1484 165 55
Fragments arranged by size:
1610 1484 358 165 55 54 11
BsrDI GCAATG_nn'
Cuts at: 1649 1649
Size: 3737
BsrFI r'CCGG_y
Cuts at: 370 548 676 1735 1916 2125 370
Size: 178 128 1059 181 209 1982
Fragments arranged by size:
1982 1059 209 181 178 128
BssSI C'ACGA_G
Cuts at: 2008 2366 2008
Size: 358 3379
BstAPI GCAn_nnn'nTGC
Cuts at: 1114 1114
Size: 3737
BstBI TT'CG_AA
Cuts at: 2098 2098
Size: 3737
BstEII G'GTnAC_C
Cuts at: 366 366
Size: 3737
BstYI r'GATC_y
Cuts at: 1 762 1229 1252 1587 1833 2845 2931 2943
Size: 761 467 23 335 246 1012 86 12
Cuts at: 2943 3289 1
Size: 346 449
Fragments arranged by size:
1012 761 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 389 107
Size: 282 3455
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1687 1868 2031 1687
Size: 181 163 3393
Fragments arranged by size:
3393 181 163
BtsI GCAGTG_nn'
Cuts at: 980 980
Size: 3737
DraI TTT'AAA
Cuts at: 736 736
Size: 3737
DrdI GACnn_nn'nnGTC
Cuts at: 1443 2301 1443
Size: 858 2879
EaeI y'GGCC_r
Cuts at: 615 1322 1496 1887 1914 3259 615
Size: 707 174 391 27 1345 1093
Fragments arranged by size:
1345 1093 707 391 174 27
EagI C'GGCC_G
Cuts at: 1322 1322
Size: 3737
EarI CTCTTCn'nnn_
Cuts at: 658 1760 1970 658
Size: 1102 210 2425
Fragments arranged by size:
2425 1102 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 367 1945 2255 2401 3547 367
Size: 1578 310 146 1146 557
Fragments arranged by size:
1578 1146 557 310 146
EcoICRI GAG'CTC
Cuts at: 164 749 164
Size: 585 3152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 1562 1994 2741 3009 432
Size: 1130 432 747 268 1160
Fragments arranged by size:
1160 1130 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 172 352 172
Size: 180 3557
EcoO109I rG'GnC_Cy
Cuts at: 384 433 708 876 384
Size: 49 275 168 3245
Fragments arranged by size:
3245 275 168 49
EcoRI G'AATT_C
Cuts at: 741 741
Size: 3737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 525 1756 525
Size: 1231 2506
FspI TGC'GCA
Cuts at: 1518 1518
Size: 3737
HaeII r_GCGC'y
Cuts at: 210 573 1171 1419 2441 3420 210
Size: 363 598 248 1022 979 527
Fragments arranged by size:
1022 979 598 527 363 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1579 1609 1611 1641 1579
Size: 30 2 30 3675
Fragments arranged by size:
3675 30 30 2
HincII GTy'rAC
Cuts at: 776 776
Size: 3737
KasI G'GCGC_C
Cuts at: 1167 1415 1167
Size: 248 3489
KpnI G_GTAC'C
Cuts at: 757 757
Size: 3737
MluI A'CGCG_T
Cuts at: 3170 3170
Size: 3737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2408 2592 2408
Size: 184 3553
MscI TGG'CCA
Cuts at: 1498 1498
Size: 3737
MslI CAynn'nnrTG
Cuts at: 1726 1853 1726
Size: 127 3610
MspA1I CmG'CkG
Cuts at: 391 848 1162 1522 2535 2780 391
Size: 457 314 360 1013 245 1348
Fragments arranged by size:
1348 1013 457 360 314 245
NaeI GCC'GGC
Cuts at: 1918 1918
Size: 3737
NarI GG'CG_CC
Cuts at: 1168 1416 1168
Size: 248 3489
NcoI C'CATG_G
Cuts at: 107 107
Size: 3737
NgoMIV G'CCGG_C
Cuts at: 1916 1916
Size: 3737
NspI r_CATG'y
Cuts at: 790 1290 2197 790
Size: 500 907 2330
Fragments arranged by size:
2330 907 500
PasI CC'CwG_GG
Cuts at: 168 168
Size: 3737
PciI A'CATG_T
Cuts at: 2193 2193
Size: 3737
PmeI GTTT'AAAC
Cuts at: 736 736
Size: 3737
PmlI CAC'GTG
Cuts at: 482 482
Size: 3737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2876 2908 2876
Size: 32 3705
PpuMI rG'GwC_Cy
Cuts at: 384 708 384
Size: 324 3413
PspOMI G'GGCC_C
Cuts at: 433 433
Size: 3737
PstI C_TGCA'G
Cuts at: 214 496 784 1469 214
Size: 282 288 685 2482
Fragments arranged by size:
2482 685 288 282
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3737
PvuII CAG'CTG
Cuts at: 1162 1522 1162
Size: 360 3377
RsrII CG'GwC_CG
Cuts at: 1932 1932
Size: 3737
SacI G_AGCT'C
Cuts at: 166 751 166
Size: 585 3152
SacII CC_GC'GG
Cuts at: 392 392
Size: 3737
SalI G'TCGA_C
Cuts at: 774 774
Size: 3737
SanDI GG'GwC_CC
Cuts at: 708 708
Size: 3737
SapI GCTCTTCn'nnn_
Cuts at: 1760 1970 1760
Size: 210 3527
SbfI CC_TGCA'GG
Cuts at: 784 784
Size: 3737
ScaI AGT'ACT
Cuts at: 3254 3254
Size: 3737
SfcI C'TryA_G
Cuts at: 33 210 492 780 1465 2458 2649 33
Size: 177 282 288 685 993 191 1121
Fragments arranged by size:
1121 993 685 288 282 191 177
SfoI GGC'GCC
Cuts at: 1169 1417 1169
Size: 248 3489
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3737
SgrAI Cr'CCGG_yG
Cuts at: 676 676
Size: 3737
SmaI CCC'GGG
Cuts at: 231 759 231
Size: 528 3209
SmlI C'TyrA_G
Cuts at: 196 892 1807 2299 2561 196
Size: 696 915 492 262 1372
Fragments arranged by size:
1372 915 696 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3737
SphI G_CATG'C
Cuts at: 790 1290 790
Size: 500 3237
StyI C'CwwG_G
Cuts at: 107 662 871 1225 107
Size: 555 209 354 2619
Fragments arranged by size:
2619 555 354 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1320 1453 1912 2121 1320
Size: 133 459 209 2936
Fragments arranged by size:
2936 459 209 133
TatI w'GTAC_w
Cuts at: 143 506 3252 143
Size: 363 2746 628
Fragments arranged by size:
2746 628 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 3705
Tth111I GACn'n_nGTC
Cuts at: 1534 1534
Size: 3737
XbaI T'CTAG_A
Cuts at: 768 768
Size: 3737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1232 1232
Size: 3737
XmaI C'CCGG_G
Cuts at: 229 757 229
Size: 528 3209
XmnI GAAnn'nnTTC
Cuts at: 259 259
Size: 3737
ZraI GAC'GTC
Cuts at: 527 527
Size: 3737
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PshAI PsiI PspXI PsrI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI