Restriction map for
pF1KB6941
(Circular) (Six-base) MAPSORT of: pF1KB6941.seq Check: 7030 from: 1 to: 3740
pF1KB6941 3740 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 778 778
Size: 3740
Acc65I G'GTAC_C
Cuts at: 756 756
Size: 3740
AclI AA'CG_TT
Cuts at: 3580 3580
Size: 3740
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 656 713 1565 1997 2744 3012 205
Size: 451 57 852 432 747 268 933
Fragments arranged by size:
933 852 747 451 432 268 57
AflII C'TTAA_G
Cuts at: 448 448
Size: 3740
AflIII A'CryG_T
Cuts at: 546 685 1092 2196 3173 546
Size: 139 407 1104 977 1113
Fragments arranged by size:
1113 1104 977 407 139
AgeI A'CCGG_T
Cuts at: 2128 2128
Size: 3740
AlwNI CAG_nnn'CTG
Cuts at: 1045 2612 1045
Size: 1567 2173
ApaLI G'TGCA_C
Cuts at: 2510 2510
Size: 3740
ApoI r'AATT_y
Cuts at: 625 744 3093 3349 625
Size: 119 2349 256 1016
Fragments arranged by size:
2349 1016 256 119
AseI AT'TA_AT
Cuts at: 20 20
Size: 3740
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
AvaI C'yCGr_G
Cuts at: 760 760
Size: 3740
BamHI G'GATC_C
Cuts at: 765 765
Size: 3740
BanI G'GyrC_C
Cuts at: 756 1170 1418 1453 756
Size: 414 248 35 3043
Fragments arranged by size:
3043 414 248 35
BanII G_rGCy'C
Cuts at: 754 1784 754
Size: 1030 2710
BbeI G_GCGC'C
Cuts at: 1174 1422 1174
Size: 248 3492
BbsI GAAGACnn'nnnn_
Cuts at: 674 674
Size: 3740
BciVI GTATCCnnnnn_n'
Cuts at: 1638 2399 3740 1638
Size: 761 1341 1638
Fragments arranged by size:
1638 1341 761
BclI T'GATC_A
Cuts at: 1260 1260
Size: 3740
BglII A'GATC_T
Cuts at: 1255 3292 1255
Size: 2037 1703
BlpI GC'TnA_GC
Cuts at: 852 852
Size: 3740
Bme1580I G_kGCm'C
Cuts at: 1365 1458 2514 1365
Size: 93 1056 2591
Fragments arranged by size:
2591 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 110 952 1033 1368 3512 110
Size: 842 81 335 2144 338
Fragments arranged by size:
2144 842 338 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 3740
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1795 2287 2585 916
Size: 879 492 298 2071
Fragments arranged by size:
2071 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3444 3444
Size: 3740
BsaAI yAC'GTr
Cuts at: 686 1093 686
Size: 407 3333
BsaBI GATnn'nnATC
Cuts at: 6 1254 3291 6
Size: 1248 2037 455
Fragments arranged by size:
2037 1248 455
BsaHI Gr'CG_yC
Cuts at: 1171 1419 2121 3617 1171
Size: 248 702 1496 1294
Fragments arranged by size:
1496 1294 702 248
BsaWI w'CCGG_w
Cuts at: 929 1450 2128 2402 2549 2960 929
Size: 521 678 274 147 411 1709
Fragments arranged by size:
1709 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 359 359
Size: 3740
BseYI C'CCAG_C
Cuts at: 1165 1923 2500 2996 1165
Size: 758 577 496 1909
Fragments arranged by size:
1909 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1282 1328 2536 104
Size: 1178 46 1208 1308
Fragments arranged by size:
1308 1208 1178 46
BsiHKAI G_wGCw'C
Cuts at: 754 1532 1722 2514 754
Size: 778 190 792 1980
Fragments arranged by size:
1980 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 754 1365 1458 1532 1722 1784 2514 754
Size: 611 93 74 190 62 730 1980
Fragments arranged by size:
1980 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2960 2960
Size: 3740
BspHI T'CATG_A
Cuts at: 2916 2916
Size: 3740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 790 1004 1306 1687 790
Size: 214 302 381 2843
Fragments arranged by size:
2843 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2032 2086 3570 3735 50
Size: 1982 54 1484 165 55
Fragments arranged by size:
1982 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1652 1652
Size: 3740
BsrFI r'CCGG_y
Cuts at: 1738 1919 2128 1738
Size: 181 209 3350
Fragments arranged by size:
3350 209 181
BsrGI T'GTAC_A
Cuts at: 549 549
Size: 3740
BssSI C'ACGA_G
Cuts at: 2011 2369 2011
Size: 358 3382
BstAPI GCAn_nnn'nTGC
Cuts at: 1117 1117
Size: 3740
BstBI TT'CG_AA
Cuts at: 2101 2101
Size: 3740
BstYI r'GATC_y
Cuts at: 1 211 765 1232 1255 1590 1836 2848 2934
Size: 210 554 467 23 335 246 1012 86
Cuts at: 2934 2946 3292 1
Size: 12 346 449
Fragments arranged by size:
1012 554 467 449 346 335 246 210
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1690 1871 2034 1690
Size: 181 163 3396
Fragments arranged by size:
3396 181 163
BtsI GCAGTG_nn'
Cuts at: 983 983
Size: 3740
DraI TTT'AAA
Cuts at: 739 739
Size: 3740
DrdI GACnn_nn'nnGTC
Cuts at: 1446 2304 1446
Size: 858 2882
EaeI y'GGCC_r
Cuts at: 1325 1499 1890 1917 3262 1325
Size: 174 391 27 1345 1803
Fragments arranged by size:
1803 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1325 1325
Size: 3740
EarI CTCTTCn'nnn_
Cuts at: 219 1763 1973 219
Size: 1544 210 1986
Fragments arranged by size:
1986 1544 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1948 2258 2404 3550 1948
Size: 310 146 1146 2138
Fragments arranged by size:
2138 1146 310 146
EcoICRI GAG'CTC
Cuts at: 752 752
Size: 3740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 656 713 1565 1997 2744 3012 205
Size: 451 57 852 432 747 268 933
Fragments arranged by size:
933 852 747 451 432 268 57
EcoNI CCTnn'n_nnAGG
Cuts at: 511 511
Size: 3740
EcoO109I rG'GnC_Cy
Cuts at: 879 879
Size: 3740
EcoRI G'AATT_C
Cuts at: 744 744
Size: 3740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1759 1759
Size: 3740
FspI TGC'GCA
Cuts at: 1521 1521
Size: 3740
HaeII r_GCGC'y
Cuts at: 436 1174 1422 2444 3423 436
Size: 738 248 1022 979 753
Fragments arranged by size:
1022 979 753 738 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1582 1612 1614 1644 1582
Size: 30 2 30 3678
Fragments arranged by size:
3678 30 30 2
HincII GTy'rAC
Cuts at: 779 779
Size: 3740
KasI G'GCGC_C
Cuts at: 1170 1418 1170
Size: 248 3492
KpnI G_GTAC'C
Cuts at: 760 760
Size: 3740
MluI A'CGCG_T
Cuts at: 3173 3173
Size: 3740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 201 2411 2595 201
Size: 2210 184 1346
Fragments arranged by size:
2210 1346 184
MscI TGG'CCA
Cuts at: 1501 1501
Size: 3740
MslI CAynn'nnrTG
Cuts at: 269 1729 1856 269
Size: 1460 127 2153
Fragments arranged by size:
2153 1460 127
MspA1I CmG'CkG
Cuts at: 851 1165 1525 2538 2783 851
Size: 314 360 1013 245 1808
Fragments arranged by size:
1808 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1921 1921
Size: 3740
NarI GG'CG_CC
Cuts at: 1171 1419 1171
Size: 248 3492
NgoMIV G'CCGG_C
Cuts at: 1919 1919
Size: 3740
NspI r_CATG'y
Cuts at: 550 793 1293 2200 550
Size: 243 500 907 2090
Fragments arranged by size:
2090 907 500 243
PasI CC'CwG_GG
Cuts at: 301 301
Size: 3740
PciI A'CATG_T
Cuts at: 546 2196 546
Size: 1650 2090
PmeI GTTT'AAAC
Cuts at: 739 739
Size: 3740
PmlI CAC'GTG
Cuts at: 686 686
Size: 3740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2879 2911 2879
Size: 32 3708
PstI C_TGCA'G
Cuts at: 787 1472 787
Size: 685 3055
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3740
PvuII CAG'CTG
Cuts at: 1165 1525 1165
Size: 360 3380
RsrII CG'GwC_CG
Cuts at: 1935 1935
Size: 3740
SacI G_AGCT'C
Cuts at: 754 754
Size: 3740
SalI G'TCGA_C
Cuts at: 777 777
Size: 3740
SapI GCTCTTCn'nnn_
Cuts at: 1763 1973 1763
Size: 210 3530
SbfI CC_TGCA'GG
Cuts at: 787 787
Size: 3740
ScaI AGT'ACT
Cuts at: 3257 3257
Size: 3740
SfcI C'TryA_G
Cuts at: 33 783 1468 2461 2652 33
Size: 750 685 993 191 1121
Fragments arranged by size:
1121 993 750 685 191
SfoI GGC'GCC
Cuts at: 1172 1420 1172
Size: 248 3492
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3740
SmaI CCC'GGG
Cuts at: 762 762
Size: 3740
SmlI C'TyrA_G
Cuts at: 448 895 1810 2302 2564 448
Size: 447 915 492 262 1624
Fragments arranged by size:
1624 915 492 447 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3740
SphI G_CATG'C
Cuts at: 793 1293 793
Size: 500 3240
StyI C'CwwG_G
Cuts at: 874 1228 874
Size: 354 3386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 367 1323 1456 1915 2124 367
Size: 956 133 459 209 1983
Fragments arranged by size:
1983 956 459 209 133
TatI w'GTAC_w
Cuts at: 549 3255 549
Size: 2706 1034
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 3708
Tth111I GACn'n_nGTC
Cuts at: 1537 1537
Size: 3740
XbaI T'CTAG_A
Cuts at: 771 771
Size: 3740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1235 1235
Size: 3740
XmaI C'CCGG_G
Cuts at: 760 760
Size: 3740
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI