Restriction map for
pF1KB6942
(Circular) (Six-base) MAPSORT of: pF1KB6942.seq Check: 1598 from: 1 to: 3800
pF1KB6942 3800 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 255 255
Size: 3800
AccI GT'mk_AC
Cuts at: 838 838
Size: 3800
Acc65I G'GTAC_C
Cuts at: 816 816
Size: 3800
AclI AA'CG_TT
Cuts at: 3640 3640
Size: 3800
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1625 2057 2804 3072 793
Size: 832 432 747 268 1521
Fragments arranged by size:
1521 832 747 432 268
AflIII A'CryG_T
Cuts at: 1152 2256 3233 1152
Size: 1104 977 1719
Fragments arranged by size:
1719 1104 977
AgeI A'CCGG_T
Cuts at: 2188 2188
Size: 3800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 620 620
Size: 3800
AlwNI CAG_nnn'CTG
Cuts at: 607 1105 2672 607
Size: 498 1567 1735
Fragments arranged by size:
1735 1567 498
ApaI G_GGCC'C
Cuts at: 283 283
Size: 3800
ApaLI G'TGCA_C
Cuts at: 2570 2570
Size: 3800
ApoI r'AATT_y
Cuts at: 804 3153 3409 804
Size: 2349 256 1195
Fragments arranged by size:
2349 1195 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3800
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
AvaI C'yCGr_G
Cuts at: 820 820
Size: 3800
BamHI G'GATC_C
Cuts at: 825 825
Size: 3800
BanI G'GyrC_C
Cuts at: 148 243 816 1230 1478 1513 148
Size: 95 573 414 248 35 2435
Fragments arranged by size:
2435 573 414 248 95 35
BanII G_rGCy'C
Cuts at: 283 814 1844 283
Size: 531 1030 2239
Fragments arranged by size:
2239 1030 531
BbeI G_GCGC'C
Cuts at: 1234 1482 1234
Size: 248 3552
BciVI GTATCCnnnnn_n'
Cuts at: 1698 2459 3800 1698
Size: 761 1341 1698
Fragments arranged by size:
1698 1341 761
BclI T'GATC_A
Cuts at: 1320 1320
Size: 3800
BglI GCCn_nnn'nGGC
Cuts at: 157 190 157
Size: 33 3767
BglII A'GATC_T
Cuts at: 1315 3352 1315
Size: 2037 1763
BlpI GC'TnA_GC
Cuts at: 912 912
Size: 3800
Bme1580I G_kGCm'C
Cuts at: 153 246 283 1425 1518 2574 153
Size: 93 37 1142 93 1056 1379
Fragments arranged by size:
1379 1142 1056 93 93 37
BmrI ACTGGGnnnn_n'
Cuts at: 1012 1093 1428 3572 1012
Size: 81 335 2144 1240
Fragments arranged by size:
2144 1240 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 3800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 3800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 1855 2347 2645 976
Size: 879 492 298 2131
Fragments arranged by size:
2131 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 658 3504 658
Size: 2846 954
BsaAI yAC'GTr
Cuts at: 624 1153 624
Size: 529 3271
BsaBI GATnn'nnATC
Cuts at: 6 1314 3351 6
Size: 1308 2037 455
Fragments arranged by size:
2037 1308 455
BsaHI Gr'CG_yC
Cuts at: 781 1231 1479 2181 3677 781
Size: 450 248 702 1496 904
Fragments arranged by size:
1496 904 702 450 248
BsaWI w'CCGG_w
Cuts at: 569 989 1510 2188 2462 2609 3020 569
Size: 420 521 678 274 147 411 1349
Fragments arranged by size:
1349 678 521 420 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 445 532 649 676 181
Size: 264 87 117 27 3305
Fragments arranged by size:
3305 264 117 87 27
BseYI C'CCAG_C
Cuts at: 194 695 1225 1983 2560 3056 194
Size: 501 530 758 577 496 938
Fragments arranged by size:
938 758 577 530 501 496
BsiEI CG_ry'CG
Cuts at: 104 182 1342 1388 2596 104
Size: 78 1160 46 1208 1308
Fragments arranged by size:
1308 1208 1160 78 46
BsiHKAI G_wGCw'C
Cuts at: 764 814 1592 1782 2574 764
Size: 50 778 190 792 1990
Fragments arranged by size:
1990 792 778 190 50
BsmI GAATG_Cn'
Cuts at: 586 586
Size: 3800
Bsp1286I G_dGCh'C
Cuts at: 153 246 283 764 814 1425 1518 1592 1782
Size: 93 37 481 50 611 93 74 190
Cuts at: 1782 1844 2574 153
Size: 62 730 1379
Fragments arranged by size:
1379 730 611 481 190 93 93 74
62 50 37
BspEI T'CCGG_A
Cuts at: 3020 3020
Size: 3800
BspHI T'CATG_A
Cuts at: 2976 2976
Size: 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 255 850 1064 1366 1747 255
Size: 595 214 302 381 2308
Fragments arranged by size:
2308 595 381 302 214
BsrBI CCG'CTC
Cuts at: 50 146 2092 2146 3630 3795 50
Size: 96 1946 54 1484 165 55
Fragments arranged by size:
1946 1484 165 96 55 54
BsrDI GCAATG_nn'
Cuts at: 374 1712 374
Size: 1338 2462
BsrFI r'CCGG_y
Cuts at: 250 1798 1979 2188 250
Size: 1548 181 209 1862
Fragments arranged by size:
1862 1548 209 181
BssSI C'ACGA_G
Cuts at: 285 2071 2429 285
Size: 1786 358 1656
Fragments arranged by size:
1786 1656 358
BstAPI GCAn_nnn'nTGC
Cuts at: 376 731 1177 376
Size: 355 446 2999
Fragments arranged by size:
2999 446 355
BstBI TT'CG_AA
Cuts at: 2161 2161
Size: 3800
BstYI r'GATC_y
Cuts at: 1 825 1292 1315 1650 1896 2908 2994 3006
Size: 824 467 23 335 246 1012 86 12
Cuts at: 3006 3352 1
Size: 346 449
Fragments arranged by size:
1012 824 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3800
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1750 1931 2094 1750
Size: 181 163 3456
Fragments arranged by size:
3456 181 163
BtsI GCAGTG_nn'
Cuts at: 736 1043 736
Size: 307 3493
DraI TTT'AAA
Cuts at: 799 799
Size: 3800
DraIII CAC_nnn'GTG
Cuts at: 680 680
Size: 3800
DrdI GACnn_nn'nnGTC
Cuts at: 786 1506 2364 786
Size: 720 858 2222
Fragments arranged by size:
2222 858 720
EaeI y'GGCC_r
Cuts at: 179 1385 1559 1950 1977 3322 179
Size: 1206 174 391 27 1345 657
Fragments arranged by size:
1345 1206 657 391 174 27
EagI C'GGCC_G
Cuts at: 179 1385 179
Size: 1206 2594
EarI CTCTTCn'nnn_
Cuts at: 1823 2033 1823
Size: 210 3590
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 178 2008 2318 2464 3610 178
Size: 1830 310 146 1146 368
Fragments arranged by size:
1830 1146 368 310 146
EcoICRI GAG'CTC
Cuts at: 812 812
Size: 3800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 793 1625 2057 2804 3072 535
Size: 258 832 432 747 268 1263
Fragments arranged by size:
1263 832 747 432 268 258
EcoO109I rG'GnC_Cy
Cuts at: 171 280 510 939 171
Size: 109 230 429 3032
Fragments arranged by size:
3032 429 230 109
EcoRI G'AATT_C
Cuts at: 804 804
Size: 3800
EcoRV GAT'ATC
Cuts at: 612 612
Size: 3800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1819 1819
Size: 3800
FspI TGC'GCA
Cuts at: 1581 1581
Size: 3800
HaeII r_GCGC'y
Cuts at: 534 1234 1482 2504 3483 534
Size: 700 248 1022 979 851
Fragments arranged by size:
1022 979 851 700 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 1642 1672 1674 1704 451
Size: 32 1159 30 2 30 2547
Fragments arranged by size:
2547 1159 32 30 30 2
HincII GTy'rAC
Cuts at: 839 839
Size: 3800
KasI G'GCGC_C
Cuts at: 1230 1478 1230
Size: 248 3552
KpnI G_GTAC'C
Cuts at: 820 820
Size: 3800
MluI A'CGCG_T
Cuts at: 3233 3233
Size: 3800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 119 2471 2655 119
Size: 2352 184 1264
Fragments arranged by size:
2352 1264 184
MscI TGG'CCA
Cuts at: 1561 1561
Size: 3800
MslI CAynn'nnrTG
Cuts at: 1789 1916 1789
Size: 127 3673
MspA1I CmG'CkG
Cuts at: 911 1225 1585 2598 2843 911
Size: 314 360 1013 245 1868
Fragments arranged by size:
1868 1013 360 314 245
NaeI GCC'GGC
Cuts at: 252 1981 252
Size: 1729 2071
NarI GG'CG_CC
Cuts at: 1231 1479 1231
Size: 248 3552
NcoI C'CATG_G
Cuts at: 107 107
Size: 3800
NgoMIV G'CCGG_C
Cuts at: 250 1979 250
Size: 1729 2071
NotI GC'GGCC_GC
Cuts at: 179 179
Size: 3800
NspI r_CATG'y
Cuts at: 853 1353 2260 853
Size: 500 907 2393
Fragments arranged by size:
2393 907 500
PciI A'CATG_T
Cuts at: 2256 2256
Size: 3800
PmeI GTTT'AAAC
Cuts at: 799 799
Size: 3800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2939 2971 2939
Size: 32 3768
PpuMI rG'GwC_Cy
Cuts at: 171 510 171
Size: 339 3461
PshAI GACnn'nnGTC
Cuts at: 319 319
Size: 3800
PspOMI G'GGCC_C
Cuts at: 279 279
Size: 3800
PstI C_TGCA'G
Cuts at: 847 1532 847
Size: 685 3115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3800
PvuII CAG'CTG
Cuts at: 1225 1585 1225
Size: 360 3440
RsrII CG'GwC_CG
Cuts at: 1995 1995
Size: 3800
SacI G_AGCT'C
Cuts at: 814 814
Size: 3800
SalI G'TCGA_C
Cuts at: 837 837
Size: 3800
SapI GCTCTTCn'nnn_
Cuts at: 1823 2033 1823
Size: 210 3590
SbfI CC_TGCA'GG
Cuts at: 847 847
Size: 3800
ScaI AGT'ACT
Cuts at: 3317 3317
Size: 3800
SfcI C'TryA_G
Cuts at: 33 843 1528 2521 2712 33
Size: 810 685 993 191 1121
Fragments arranged by size:
1121 993 810 685 191
SfoI GGC'GCC
Cuts at: 1232 1480 1232
Size: 248 3552
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
SmaI CCC'GGG
Cuts at: 822 822
Size: 3800
SmlI C'TyrA_G
Cuts at: 955 1870 2362 2624 955
Size: 915 492 262 2131
Fragments arranged by size:
2131 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3800
SphI G_CATG'C
Cuts at: 853 1353 853
Size: 500 3300
StyI C'CwwG_G
Cuts at: 107 656 934 1288 107
Size: 549 278 354 2619
Fragments arranged by size:
2619 549 354 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1383 1516 1975 2184 1383
Size: 133 459 209 2999
Fragments arranged by size:
2999 459 209 133
TatI w'GTAC_w
Cuts at: 3315 3315
Size: 3800
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 3768
Tth111I GACn'n_nGTC
Cuts at: 1597 1597
Size: 3800
XbaI T'CTAG_A
Cuts at: 831 831
Size: 3800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 510 677 1295 510
Size: 167 618 3015
Fragments arranged by size:
3015 618 167
XmaI C'CCGG_G
Cuts at: 820 820
Size: 3800
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PasI
PflMI PfoI PmlI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI