Restriction map for
pF1KB6943
(Circular) (Six-base) MAPSORT of: pF1KB6943.seq Check: 4164 from: 1 to: 3662
pF1KB6943 3662 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 700 700
Size: 3662
Acc65I G'GTAC_C
Cuts at: 678 678
Size: 3662
AclI AA'CG_TT
Cuts at: 3502 3502
Size: 3662
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1487 1919 2666 2934 1487
Size: 432 747 268 2215
Fragments arranged by size:
2215 747 432 268
AfeI AGC'GCT
Cuts at: 134 134
Size: 3662
AflIII A'CryG_T
Cuts at: 407 1014 2118 3095 407
Size: 607 1104 977 974
Fragments arranged by size:
1104 977 974 607
AgeI A'CCGG_T
Cuts at: 2050 2050
Size: 3662
AlwNI CAG_nnn'CTG
Cuts at: 967 2534 967
Size: 1567 2095
ApaLI G'TGCA_C
Cuts at: 2432 2432
Size: 3662
ApoI r'AATT_y
Cuts at: 666 3015 3271 666
Size: 2349 256 1057
Fragments arranged by size:
2349 1057 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3662
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
AvaI C'yCGr_G
Cuts at: 266 321 682 266
Size: 55 361 3246
Fragments arranged by size:
3246 361 55
BamHI G'GATC_C
Cuts at: 687 687
Size: 3662
BanI G'GyrC_C
Cuts at: 273 678 1092 1340 1375 273
Size: 405 414 248 35 2560
Fragments arranged by size:
2560 414 405 248 35
BanII G_rGCy'C
Cuts at: 583 676 1706 583
Size: 93 1030 2539
Fragments arranged by size:
2539 1030 93
BbeI G_GCGC'C
Cuts at: 277 1096 1344 277
Size: 819 248 2595
Fragments arranged by size:
2595 819 248
BbsI GAAGACnn'nnnn_
Cuts at: 607 639 607
Size: 32 3630
BciVI GTATCCnnnnn_n'
Cuts at: 1560 2321 3662 1560
Size: 761 1341 1560
Fragments arranged by size:
1560 1341 761
BclI T'GATC_A
Cuts at: 1182 1182
Size: 3662
BglI GCCn_nnn'nGGC
Cuts at: 271 271
Size: 3662
BglII A'GATC_T
Cuts at: 1177 3214 1177
Size: 2037 1625
BlpI GC'TnA_GC
Cuts at: 774 774
Size: 3662
Bme1580I G_kGCm'C
Cuts at: 1287 1380 2436 1287
Size: 93 1056 2513
Fragments arranged by size:
2513 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 874 955 1290 3434 874
Size: 81 335 2144 1102
Fragments arranged by size:
2144 1102 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 543 2029 543
Size: 1486 2176
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 253 482 488 214
Size: 39 229 6 3388
Fragments arranged by size:
3388 229 39 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1717 2209 2507 838
Size: 879 492 298 1993
Fragments arranged by size:
1993 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3366 3366
Size: 3662
BsaAI yAC'GTr
Cuts at: 1015 1015
Size: 3662
BsaBI GATnn'nnATC
Cuts at: 6 1176 3213 6
Size: 1170 2037 455
Fragments arranged by size:
2037 1170 455
BsaHI Gr'CG_yC
Cuts at: 274 1093 1341 2043 3539 274
Size: 819 248 702 1496 397
Fragments arranged by size:
1496 819 702 397 248
BsaWI w'CCGG_w
Cuts at: 851 1372 2050 2324 2471 2882 851
Size: 521 678 274 147 411 1631
Fragments arranged by size:
1631 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 222 222
Size: 3662
BseYI C'CCAG_C
Cuts at: 509 1087 1845 2422 2918 509
Size: 578 758 577 496 1253
Fragments arranged by size:
1253 758 578 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 388
Size: 3662
BsiEI CG_ry'CG
Cuts at: 104 128 1204 1250 2458 104
Size: 24 1076 46 1208 1308
Fragments arranged by size:
1308 1208 1076 46 24
BsiHKAI G_wGCw'C
Cuts at: 676 1454 1644 2436 676
Size: 778 190 792 1902
Fragments arranged by size:
1902 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 583 676 1287 1380 1454 1644 1706 2436 583
Size: 93 611 93 74 190 62 730 1809
Fragments arranged by size:
1809 730 611 190 93 93 74 62
BspEI T'CCGG_A
Cuts at: 2882 2882
Size: 3662
BspHI T'CATG_A
Cuts at: 2838 2838
Size: 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 712 926 1228 1609 712
Size: 214 302 381 2765
Fragments arranged by size:
2765 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1954 2008 3492 3657 50
Size: 1904 54 1484 165 55
Fragments arranged by size:
1904 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1574 1574
Size: 3662
BsrFI r'CCGG_y
Cuts at: 294 301 1660 1841 2050 294
Size: 7 1359 181 209 1906
Fragments arranged by size:
1906 1359 209 181 7
BssSI C'ACGA_G
Cuts at: 1933 2291 1933
Size: 358 3304
BstAPI GCAn_nnn'nTGC
Cuts at: 1039 1039
Size: 3662
BstBI TT'CG_AA
Cuts at: 2023 2023
Size: 3662
BstYI r'GATC_y
Cuts at: 1 687 1154 1177 1512 1758 2770 2856 2868
Size: 686 467 23 335 246 1012 86 12
Cuts at: 2868 3214 1
Size: 346 449
Fragments arranged by size:
1012 686 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1612 1793 1956 1612
Size: 181 163 3318
Fragments arranged by size:
3318 181 163
BtsI GCAGTG_nn'
Cuts at: 905 905
Size: 3662
DraI TTT'AAA
Cuts at: 661 661
Size: 3662
DrdI GACnn_nn'nnGTC
Cuts at: 1368 2226 1368
Size: 858 2804
EaeI y'GGCC_r
Cuts at: 125 1247 1421 1812 1839 3184 125
Size: 1122 174 391 27 1345 603
Fragments arranged by size:
1345 1122 603 391 174 27
EagI C'GGCC_G
Cuts at: 125 1247 125
Size: 1122 2540
EarI CTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1870 2180 2326 3472 1870
Size: 310 146 1146 2060
Fragments arranged by size:
2060 1146 310 146
EcoICRI GAG'CTC
Cuts at: 674 674
Size: 3662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 253 482 488 1487 1919 2666 2934 214
Size: 39 229 6 999 432 747 268 942
Fragments arranged by size:
999 942 747 432 268 229 39 6
EcoO109I rG'GnC_Cy
Cuts at: 258 534 560 801 258
Size: 276 26 241 3119
Fragments arranged by size:
3119 276 241 26
EcoRI G'AATT_C
Cuts at: 666 666
Size: 3662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1681 1681
Size: 3662
FspI TGC'GCA
Cuts at: 1443 1443
Size: 3662
HaeII r_GCGC'y
Cuts at: 136 277 1096 1344 2366 3345 136
Size: 141 819 248 1022 979 453
Fragments arranged by size:
1022 979 819 453 248 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1504 1534 1536 1566 1504
Size: 30 2 30 3600
Fragments arranged by size:
3600 30 30 2
HincII GTy'rAC
Cuts at: 701 701
Size: 3662
KasI G'GCGC_C
Cuts at: 273 1092 1340 273
Size: 819 248 2595
Fragments arranged by size:
2595 819 248
KpnI G_GTAC'C
Cuts at: 682 682
Size: 3662
MluI A'CGCG_T
Cuts at: 3095 3095
Size: 3662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2333 2517 2333
Size: 184 3478
MscI TGG'CCA
Cuts at: 1423 1423
Size: 3662
MslI CAynn'nnrTG
Cuts at: 1651 1778 1651
Size: 127 3535
MspA1I CmG'CkG
Cuts at: 773 1087 1447 2460 2705 773
Size: 314 360 1013 245 1730
Fragments arranged by size:
1730 1013 360 314 245
NaeI GCC'GGC
Cuts at: 296 303 1843 296
Size: 7 1540 2115
Fragments arranged by size:
2115 1540 7
NarI GG'CG_CC
Cuts at: 274 1093 1341 274
Size: 819 248 2595
Fragments arranged by size:
2595 819 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3662
NgoMIV G'CCGG_C
Cuts at: 294 301 1841 294
Size: 7 1540 2115
Fragments arranged by size:
2115 1540 7
NotI GC'GGCC_GC
Cuts at: 125 125
Size: 3662
NspI r_CATG'y
Cuts at: 229 411 496 715 1215 2122 229
Size: 182 85 219 500 907 1769
Fragments arranged by size:
1769 907 500 219 182 85
PciI A'CATG_T
Cuts at: 407 2118 407
Size: 1711 1951
PfoI T'CCnGG_A
Cuts at: 465 465
Size: 3662
PmeI GTTT'AAAC
Cuts at: 661 661
Size: 3662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2801 2833 2801
Size: 32 3630
PpuMI rG'GwC_Cy
Cuts at: 258 534 560 258
Size: 276 26 3360
Fragments arranged by size:
3360 276 26
PstI C_TGCA'G
Cuts at: 164 709 1394 164
Size: 545 685 2432
Fragments arranged by size:
2432 685 545
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3662
PvuII CAG'CTG
Cuts at: 1087 1447 1087
Size: 360 3302
RsrII CG'GwC_CG
Cuts at: 1857 1857
Size: 3662
SacI G_AGCT'C
Cuts at: 676 676
Size: 3662
SalI G'TCGA_C
Cuts at: 699 699
Size: 3662
SapI GCTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
SbfI CC_TGCA'GG
Cuts at: 709 709
Size: 3662
ScaI AGT'ACT
Cuts at: 3179 3179
Size: 3662
SexAI A'CCwGG_T
Cuts at: 641 641
Size: 3662
SfcI C'TryA_G
Cuts at: 33 160 705 1390 2383 2574 33
Size: 127 545 685 993 191 1121
Fragments arranged by size:
1121 993 685 545 191 127
SfoI GGC'GCC
Cuts at: 275 1094 1342 275
Size: 819 248 2595
Fragments arranged by size:
2595 819 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
SmaI CCC'GGG
Cuts at: 268 323 684 268
Size: 55 361 3246
Fragments arranged by size:
3246 361 55
SmlI C'TyrA_G
Cuts at: 817 1732 2224 2486 817
Size: 915 492 262 1993
Fragments arranged by size:
1993 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3662
SphI G_CATG'C
Cuts at: 229 496 715 1215 229
Size: 267 219 500 2676
Fragments arranged by size:
2676 500 267 219
SspI AAT'ATT
Cuts at: 334 334
Size: 3662
StyI C'CwwG_G
Cuts at: 107 459 796 1150 107
Size: 352 337 354 2619
Fragments arranged by size:
2619 354 352 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 164 1245 1378 1837 2046 164
Size: 1081 133 459 209 1780
Fragments arranged by size:
1780 1081 459 209 133
TatI w'GTAC_w
Cuts at: 3177 3177
Size: 3662
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 620 652 3179 3211 620
Size: 32 2527 32 1071
Fragments arranged by size:
2527 1071 32 32
Tth111I GACn'n_nGTC
Cuts at: 643 1459 643
Size: 816 2846
XbaI T'CTAG_A
Cuts at: 183 693 183
Size: 510 3152
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 249 1157 249
Size: 908 2754
XmaI C'CCGG_G
Cuts at: 266 321 682 266
Size: 55 361 3246
Fragments arranged by size:
3246 361 55
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI