Restriction map for
pF1KB6949
(Circular) (Six-base) MAPSORT of: pF1KB6949.seq Check: 4538 from: 1 to: 3746
pF1KB6949 3746 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 689 762 689
Size: 73 3673
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
AflIII A'CryG_T
Cuts at: 1098 2202 3179 1098
Size: 1104 977 1665
Fragments arranged by size:
1665 1104 977
AgeI A'CCGG_T
Cuts at: 2134 2134
Size: 3746
AlwNI CAG_nnn'CTG
Cuts at: 132 622 1051 2618 132
Size: 490 429 1567 1260
Fragments arranged by size:
1567 1260 490 429
ApaLI G'TGCA_C
Cuts at: 417 644 695 2516 417
Size: 227 51 1821 1647
Fragments arranged by size:
1821 1647 227 51
ApoI r'AATT_y
Cuts at: 750 3099 3355 750
Size: 2349 256 1141
Fragments arranged by size:
2349 1141 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 347 766 347
Size: 419 3327
BamHI G'GATC_C
Cuts at: 771 771
Size: 3746
BanI G'GyrC_C
Cuts at: 689 762 1176 1424 1459 689
Size: 73 414 248 35 2976
Fragments arranged by size:
2976 414 248 73 35
BanII G_rGCy'C
Cuts at: 208 354 760 1790 208
Size: 146 406 1030 2164
Fragments arranged by size:
2164 1030 406 146
BbeI G_GCGC'C
Cuts at: 1180 1428 1180
Size: 248 3498
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 328 362 328
Size: 34 3712
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2405 3746 1644
Size: 761 1341 1644
Fragments arranged by size:
1644 1341 761
BclI T'GATC_A
Cuts at: 1266 1266
Size: 3746
BglI GCCn_nnn'nGGC
Cuts at: 340 340
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 421 648 699 1371 1464 2520 421
Size: 227 51 672 93 1056 1647
Fragments arranged by size:
1647 1056 672 227 93 51
BmrI ACTGGGnnnn_n'
Cuts at: 958 1039 1374 3518 958
Size: 81 335 2144 1186
Fragments arranged by size:
2144 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2293 2591 922
Size: 879 492 298 2077
Fragments arranged by size:
2077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 369 3450 369
Size: 3081 665
BsaAI yAC'GTr
Cuts at: 1099 1099
Size: 3746
BsaBI GATnn'nnATC
Cuts at: 6 1260 3297 6
Size: 1254 2037 455
Fragments arranged by size:
2037 1254 455
BsaHI Gr'CG_yC
Cuts at: 1177 1425 2127 3623 1177
Size: 248 702 1496 1300
Fragments arranged by size:
1496 1300 702 248
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2408 2555 2966 935
Size: 521 678 274 147 411 1715
Fragments arranged by size:
1715 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 132 153 158 375 611 685 1171 1929 2506
Size: 21 5 217 236 74 486 758 577
Cuts at: 2506 3002 132
Size: 496 876
Fragments arranged by size:
876 758 577 496 486 236 217 74
21 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 162
Size: 3746
BsiEI CG_ry'CG
Cuts at: 104 1288 1334 2542 104
Size: 1184 46 1208 1308
Fragments arranged by size:
1308 1208 1184 46
BsiHKAI G_wGCw'C
Cuts at: 208 283 320 421 648 699 760 1538 1728
Size: 75 37 101 227 51 61 778 190
Cuts at: 1728 2520 208
Size: 792 1434
Fragments arranged by size:
1434 792 778 227 190 101 75 61
51 37
BsmBI CGTCTCn'nnnn_
Cuts at: 428 428
Size: 3746
Bsp1286I G_dGCh'C
Cuts at: 208 283 320 354 421 648 699 760 1371
Size: 75 37 34 67 227 51 61 611
Cuts at: 1371 1464 1538 1728 1790 2520 208
Size: 93 74 190 62 730 1434
Fragments arranged by size:
1434 730 611 227 190 93 75 74
67 62 61 51 37 34
BspEI T'CCGG_A
Cuts at: 2966 2966
Size: 3746
BspHI T'CATG_A
Cuts at: 2922 2922
Size: 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 484 796 1010 1312 1693 484
Size: 312 214 302 381 2537
Fragments arranged by size:
2537 381 312 302 214
BsrBI CCG'CTC
Cuts at: 50 704 2038 2092 3576 3741 50
Size: 654 1334 54 1484 165 55
Fragments arranged by size:
1484 1334 654 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1658 1658
Size: 3746
BsrFI r'CCGG_y
Cuts at: 1744 1925 2134 1744
Size: 181 209 3356
Fragments arranged by size:
3356 209 181
BsrGI T'GTAC_A
Cuts at: 245 245
Size: 3746
BssSI C'ACGA_G
Cuts at: 2017 2375 2017
Size: 358 3388
BstAPI GCAn_nnn'nTGC
Cuts at: 416 499 622 1123 416
Size: 83 123 501 3039
Fragments arranged by size:
3039 501 123 83
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstXI CCAn_nnnn'nTGG
Cuts at: 396 396
Size: 3746
BstYI r'GATC_y
Cuts at: 1 771 1238 1261 1596 1842 2854 2940 2952
Size: 770 467 23 335 246 1012 86 12
Cuts at: 2952 3298 1
Size: 346 449
Fragments arranged by size:
1012 770 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 143 329 464 107
Size: 36 186 135 3389
Fragments arranged by size:
3389 186 135 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1696 1877 2040 1696
Size: 181 163 3402
Fragments arranged by size:
3402 181 163
BtsI GCAGTG_nn'
Cuts at: 199 646 989 199
Size: 447 343 2956
Fragments arranged by size:
2956 447 343
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2310 1452
Size: 858 2888
EaeI y'GGCC_r
Cuts at: 110 386 398 528 1331 1505 1896 1923 3268
Size: 276 12 130 803 174 391 27 1345
Cuts at: 3268 110
Size: 588
Fragments arranged by size:
1345 803 588 391 276 174 130 27
12
EagI C'GGCC_G
Cuts at: 1331 1331
Size: 3746
EarI CTCTTCn'nnn_
Cuts at: 729 1769 1979 729
Size: 1040 210 2496
Fragments arranged by size:
2496 1040 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1954 2264 2410 3556 1954
Size: 310 146 1146 2144
Fragments arranged by size:
2144 1146 310 146
EcoICRI GAG'CTC
Cuts at: 206 758 206
Size: 552 3194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 2003 2750 3018 1571
Size: 432 747 268 2299
Fragments arranged by size:
2299 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 885 885
Size: 3746
EcoRI G'AATT_C
Cuts at: 750 750
Size: 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1765 1765
Size: 3746
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 1180 1428 2450 3429 1180
Size: 248 1022 979 1497
Fragments arranged by size:
1497 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1588 1618 1620 1650 1588
Size: 30 2 30 3684
Fragments arranged by size:
3684 30 30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
KasI G'GCGC_C
Cuts at: 1176 1424 1176
Size: 248 3498
KpnI G_GTAC'C
Cuts at: 693 766 693
Size: 73 3673
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 743 2417 2601 743
Size: 1674 184 1888
Fragments arranged by size:
1888 1674 184
MscI TGG'CCA
Cuts at: 112 388 400 530 1507 112
Size: 276 12 130 977 2351
Fragments arranged by size:
2351 977 276 130 12
MslI CAynn'nnrTG
Cuts at: 286 394 1735 1862 286
Size: 108 1341 127 2170
Fragments arranged by size:
2170 1341 127 108
MspA1I CmG'CkG
Cuts at: 331 857 1171 1531 2544 2789 331
Size: 526 314 360 1013 245 1288
Fragments arranged by size:
1288 1013 526 360 314 245
NaeI GCC'GGC
Cuts at: 1927 1927
Size: 3746
NarI GG'CG_CC
Cuts at: 1177 1425 1177
Size: 248 3498
NcoI C'CATG_G
Cuts at: 107 143 464 107
Size: 36 321 3389
Fragments arranged by size:
3389 321 36
NgoMIV G'CCGG_C
Cuts at: 1925 1925
Size: 3746
NspI r_CATG'y
Cuts at: 799 1299 2206 799
Size: 500 907 2339
Fragments arranged by size:
2339 907 500
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 3714
PsiI TTA'TAA
Cuts at: 554 554
Size: 3746
PspXI vC'TCGA_Gb
Cuts at: 347 347
Size: 3746
PstI C_TGCA'G
Cuts at: 128 793 1478 128
Size: 665 685 2396
Fragments arranged by size:
2396 685 665
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 1171 1531 1171
Size: 360 3386
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 208 760 208
Size: 552 3194
SacII CC_GC'GG
Cuts at: 332 332
Size: 3746
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 124 789 1474 2467 2658 33
Size: 91 665 685 993 191 1121
Fragments arranged by size:
1121 993 685 665 191 91
SfiI GGCCn_nnn'nGGCC
Cuts at: 340 340
Size: 3746
SfoI GGC'GCC
Cuts at: 1178 1426 1178
Size: 248 3498
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 347 901 1816 2308 2570 347
Size: 554 915 492 262 1523
Fragments arranged by size:
1523 915 554 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
StyI C'CwwG_G
Cuts at: 107 143 464 506 728 880 1234 107
Size: 36 321 42 222 152 354 2619
Fragments arranged by size:
2619 354 321 222 152 42 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 150 457 1329 1462 1921 2130 150
Size: 307 872 133 459 209 1766
Fragments arranged by size:
1766 872 459 307 209 133
TatI w'GTAC_w
Cuts at: 245 3261 245
Size: 3016 730
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 268 1543 268
Size: 1275 2471
XbaI T'CTAG_A
Cuts at: 777 777
Size: 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 735 1241 150
Size: 585 506 2655
Fragments arranged by size:
2655 585 506
XhoI C'TCGA_G
Cuts at: 347 347
Size: 3746
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PsiI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PsrI SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI