Restriction map for
pF1KB6952
(Circular) (Six-base) MAPSORT of: pF1KB6952.seq Check: 4716 from: 1 to: 3818
pF1KB6952 3818 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 856 856
Size: 3818
Acc65I G'GTAC_C
Cuts at: 834 834
Size: 3818
AclI AA'CG_TT
Cuts at: 3658 3658
Size: 3818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 1643 2075 2822 3090 359
Size: 1284 432 747 268 1087
Fragments arranged by size:
1284 1087 747 432 268
AflIII A'CryG_T
Cuts at: 676 1170 2274 3251 676
Size: 494 1104 977 1243
Fragments arranged by size:
1243 1104 977 494
AgeI A'CCGG_T
Cuts at: 2206 2206
Size: 3818
AlwNI CAG_nnn'CTG
Cuts at: 1123 2690 1123
Size: 1567 2251
ApaLI G'TGCA_C
Cuts at: 2588 2588
Size: 3818
ApoI r'AATT_y
Cuts at: 427 448 497 529 775 822 3171 3427 427
Size: 21 49 32 246 47 2349 256 818
Fragments arranged by size:
2349 818 256 246 49 47 32 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 3818
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
AvaI C'yCGr_G
Cuts at: 838 838
Size: 3818
BamHI G'GATC_C
Cuts at: 843 843
Size: 3818
BanI G'GyrC_C
Cuts at: 834 1248 1496 1531 834
Size: 414 248 35 3121
Fragments arranged by size:
3121 414 248 35
BanII G_rGCy'C
Cuts at: 832 1862 832
Size: 1030 2788
BbeI G_GCGC'C
Cuts at: 1252 1500 1252
Size: 248 3570
BbsI GAAGACnn'nnnn_
Cuts at: 247 290 726 247
Size: 43 436 3339
Fragments arranged by size:
3339 436 43
BciVI GTATCCnnnnn_n'
Cuts at: 1716 2477 3818 1716
Size: 761 1341 1716
Fragments arranged by size:
1716 1341 761
BclI T'GATC_A
Cuts at: 1338 1338
Size: 3818
BglII A'GATC_T
Cuts at: 301 1333 3370 301
Size: 1032 2037 749
Fragments arranged by size:
2037 1032 749
BlpI GC'TnA_GC
Cuts at: 930 930
Size: 3818
Bme1580I G_kGCm'C
Cuts at: 311 1443 1536 2592 311
Size: 1132 93 1056 1537
Fragments arranged by size:
1537 1132 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1030 1111 1446 3590 1030
Size: 81 335 2144 1258
Fragments arranged by size:
2144 1258 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2185 2185
Size: 3818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 279
Size: 3818
Bpu10I CC'TnA_GC
Cuts at: 230 230
Size: 3818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 1873 2365 2663 994
Size: 879 492 298 2149
Fragments arranged by size:
2149 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 723 3522 723
Size: 2799 1019
BsaAI yAC'GTr
Cuts at: 1171 1171
Size: 3818
BsaBI GATnn'nnATC
Cuts at: 6 1332 3369 6
Size: 1326 2037 455
Fragments arranged by size:
2037 1326 455
BsaHI Gr'CG_yC
Cuts at: 1249 1497 2199 3695 1249
Size: 248 702 1496 1372
Fragments arranged by size:
1496 1372 702 248
BsaWI w'CCGG_w
Cuts at: 1007 1528 2206 2480 2627 3038 1007
Size: 521 678 274 147 411 1787
Fragments arranged by size:
1787 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 277 307 599 629 277
Size: 30 292 30 3466
Fragments arranged by size:
3466 292 30 30
BseYI C'CCAG_C
Cuts at: 1243 2001 2578 3074 1243
Size: 758 577 496 1987
Fragments arranged by size:
1987 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 617 283
Size: 334 3484
BsiEI CG_ry'CG
Cuts at: 104 1360 1406 2614 104
Size: 1256 46 1208 1308
Fragments arranged by size:
1308 1256 1208 46
BsiHKAI G_wGCw'C
Cuts at: 832 1610 1800 2592 832
Size: 778 190 792 2058
Fragments arranged by size:
2058 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 741 741
Size: 3818
Bsp1286I G_dGCh'C
Cuts at: 311 832 1443 1536 1610 1800 1862 2592 311
Size: 521 611 93 74 190 62 730 1537
Fragments arranged by size:
1537 730 611 521 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3038 3038
Size: 3818
BspHI T'CATG_A
Cuts at: 279 2994 279
Size: 2715 1103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 775 868 1082 1384 1765 775
Size: 93 214 302 381 2828
Fragments arranged by size:
2828 381 302 214 93
BsrBI CCG'CTC
Cuts at: 50 293 2110 2164 3648 3813 50
Size: 243 1817 54 1484 165 55
Fragments arranged by size:
1817 1484 243 165 55 54
BsrDI GCAATG_nn'
Cuts at: 118 777 1730 118
Size: 659 953 2206
Fragments arranged by size:
2206 953 659
BsrFI r'CCGG_y
Cuts at: 1816 1997 2206 1816
Size: 181 209 3428
Fragments arranged by size:
3428 209 181
BsrGI T'GTAC_A
Cuts at: 786 786
Size: 3818
BssSI C'ACGA_G
Cuts at: 2089 2447 2089
Size: 358 3460
BstAPI GCAn_nnn'nTGC
Cuts at: 1195 1195
Size: 3818
BstBI TT'CG_AA
Cuts at: 2179 2179
Size: 3818
BstXI CCAn_nnnn'nTGG
Cuts at: 391 391
Size: 3818
BstYI r'GATC_y
Cuts at: 1 301 843 1310 1333 1668 1914 2926 3012
Size: 300 542 467 23 335 246 1012 86
Cuts at: 3012 3024 3370 1
Size: 12 346 449
Fragments arranged by size:
1012 542 467 449 346 335 300 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3818
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1768 1949 2112 1768
Size: 181 163 3474
Fragments arranged by size:
3474 181 163
BtsI GCAGTG_nn'
Cuts at: 353 1061 353
Size: 708 3110
DraI TTT'AAA
Cuts at: 817 817
Size: 3818
DrdI GACnn_nn'nnGTC
Cuts at: 1524 2382 1524
Size: 858 2960
EaeI y'GGCC_r
Cuts at: 314 1403 1577 1968 1995 3340 314
Size: 1089 174 391 27 1345 792
Fragments arranged by size:
1345 1089 792 391 174 27
EagI C'GGCC_G
Cuts at: 1403 1403
Size: 3818
EarI CTCTTCn'nnn_
Cuts at: 335 1841 2051 335
Size: 1506 210 2102
Fragments arranged by size:
2102 1506 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2026 2336 2482 3628 2026
Size: 310 146 1146 2216
Fragments arranged by size:
2216 1146 310 146
EcoICRI GAG'CTC
Cuts at: 830 830
Size: 3818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 359 1643 2075 2822 3090 279
Size: 80 1284 432 747 268 1007
Fragments arranged by size:
1284 1007 747 432 268 80
EcoO109I rG'GnC_Cy
Cuts at: 487 957 487
Size: 470 3348
EcoRI G'AATT_C
Cuts at: 427 775 822 427
Size: 348 47 3423
Fragments arranged by size:
3423 348 47
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1837 1837
Size: 3818
FspI TGC'GCA
Cuts at: 1599 1599
Size: 3818
HaeII r_GCGC'y
Cuts at: 1252 1500 2522 3501 1252
Size: 248 1022 979 1569
Fragments arranged by size:
1569 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 277 309 775 807 1660 1690 1692 1722 277
Size: 32 466 32 853 30 2 30 2373
Fragments arranged by size:
2373 853 466 32 32 30 30 2
HincII GTy'rAC
Cuts at: 561 857 561
Size: 296 3522
HpaI GTT'AAC
Cuts at: 561 561
Size: 3818
KasI G'GCGC_C
Cuts at: 1248 1496 1248
Size: 248 3570
KpnI G_GTAC'C
Cuts at: 838 838
Size: 3818
MluI A'CGCG_T
Cuts at: 3251 3251
Size: 3818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2489 2673 2489
Size: 184 3634
MscI TGG'CCA
Cuts at: 316 1579 316
Size: 1263 2555
MslI CAynn'nnrTG
Cuts at: 389 1807 1934 389
Size: 1418 127 2273
Fragments arranged by size:
2273 1418 127
MspA1I CmG'CkG
Cuts at: 929 1243 1603 2616 2861 929
Size: 314 360 1013 245 1886
Fragments arranged by size:
1886 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1999 1999
Size: 3818
NarI GG'CG_CC
Cuts at: 1249 1497 1249
Size: 248 3570
NcoI C'CATG_G
Cuts at: 107 107
Size: 3818
NgoMIV G'CCGG_C
Cuts at: 1997 1997
Size: 3818
NspI r_CATG'y
Cuts at: 871 1371 2278 871
Size: 500 907 2411
Fragments arranged by size:
2411 907 500
PciI A'CATG_T
Cuts at: 2274 2274
Size: 3818
PmeI GTTT'AAAC
Cuts at: 817 817
Size: 3818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 3786
PpuMI rG'GwC_Cy
Cuts at: 487 487
Size: 3818
PstI C_TGCA'G
Cuts at: 865 1550 865
Size: 685 3133
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3818
PvuII CAG'CTG
Cuts at: 1243 1603 1243
Size: 360 3458
RsrII CG'GwC_CG
Cuts at: 2013 2013
Size: 3818
SacI G_AGCT'C
Cuts at: 832 832
Size: 3818
SalI G'TCGA_C
Cuts at: 855 855
Size: 3818
SapI GCTCTTCn'nnn_
Cuts at: 1841 2051 1841
Size: 210 3608
SbfI CC_TGCA'GG
Cuts at: 865 865
Size: 3818
ScaI AGT'ACT
Cuts at: 3335 3335
Size: 3818
SfcI C'TryA_G
Cuts at: 33 861 1546 2539 2730 33
Size: 828 685 993 191 1121
Fragments arranged by size:
1121 993 828 685 191
SfoI GGC'GCC
Cuts at: 1250 1498 1250
Size: 248 3570
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3818
SmaI CCC'GGG
Cuts at: 840 840
Size: 3818
SmlI C'TyrA_G
Cuts at: 973 1888 2380 2642 973
Size: 915 492 262 2149
Fragments arranged by size:
2149 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3818
SphI G_CATG'C
Cuts at: 871 1371 871
Size: 500 3318
SspI AAT'ATT
Cuts at: 444 444
Size: 3818
StyI C'CwwG_G
Cuts at: 107 952 1306 107
Size: 845 354 2619
Fragments arranged by size:
2619 845 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1401 1534 1993 2202 1401
Size: 133 459 209 3017
Fragments arranged by size:
3017 459 209 133
TatI w'GTAC_w
Cuts at: 786 3333 786
Size: 2547 1271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 447 479 3335 3367 447
Size: 32 2856 32 898
Fragments arranged by size:
2856 898 32 32
Tth111I GACn'n_nGTC
Cuts at: 251 1615 251
Size: 1364 2454
XbaI T'CTAG_A
Cuts at: 849 849
Size: 3818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1313 1313
Size: 3818
XmaI C'CCGG_G
Cuts at: 838 838
Size: 3818
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BseRI BsiWI BsmI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI