Restriction map for
pF1KB6953
(Circular) (Six-base) MAPSORT of: pF1KB6953.seq Check: 725 from: 1 to: 3749
pF1KB6953 3749 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 299 787 299
Size: 488 3261
Acc65I G'GTAC_C
Cuts at: 765 765
Size: 3749
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 1574 2006 2753 3021 521
Size: 1053 432 747 268 1249
Fragments arranged by size:
1249 1053 747 432 268
AfeI AGC'GCT
Cuts at: 262 262
Size: 3749
AflIII A'CryG_T
Cuts at: 1101 2205 3182 1101
Size: 1104 977 1668
Fragments arranged by size:
1668 1104 977
AgeI A'CCGG_T
Cuts at: 2137 2137
Size: 3749
AlwNI CAG_nnn'CTG
Cuts at: 1054 2621 1054
Size: 1567 2182
ApaLI G'TGCA_C
Cuts at: 2519 2519
Size: 3749
ApoI r'AATT_y
Cuts at: 393 468 753 3102 3358 393
Size: 75 285 2349 256 784
Fragments arranged by size:
2349 784 285 256 75
AseI AT'TA_AT
Cuts at: 20 20
Size: 3749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 337 769 337
Size: 432 3317
AvrII C'CTAG_G
Cuts at: 370 370
Size: 3749
BamHI G'GATC_C
Cuts at: 774 774
Size: 3749
BanI G'GyrC_C
Cuts at: 765 1179 1427 1462 765
Size: 414 248 35 3052
Fragments arranged by size:
3052 414 248 35
BanII G_rGCy'C
Cuts at: 162 191 763 1793 162
Size: 29 572 1030 2118
Fragments arranged by size:
2118 1030 572 29
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BbsI GAAGACnn'nnnn_
Cuts at: 509 509
Size: 3749
BciVI GTATCCnnnnn_n'
Cuts at: 1647 2408 3749 1647
Size: 761 1341 1647
Fragments arranged by size:
1647 1341 761
BclI T'GATC_A
Cuts at: 1269 1269
Size: 3749
BglII A'GATC_T
Cuts at: 1264 3301 1264
Size: 2037 1712
BlpI GC'TnA_GC
Cuts at: 185 190 861 185
Size: 5 671 3073
Fragments arranged by size:
3073 671 5
Bme1580I G_kGCm'C
Cuts at: 1374 1467 2523 1374
Size: 93 1056 2600
Fragments arranged by size:
2600 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 224 961 1042 1377 3521 224
Size: 737 81 335 2144 452
Fragments arranged by size:
2144 737 452 335 81
BmtI G_CTAG'C
Cuts at: 446 446
Size: 3749
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 3749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 690 146
Size: 544 3205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1804 2296 2594 925
Size: 879 492 298 2080
Fragments arranged by size:
2080 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 640 3453 640
Size: 2813 936
BsaAI yAC'GTr
Cuts at: 1102 1102
Size: 3749
BsaBI GATnn'nnATC
Cuts at: 6 1263 3300 6
Size: 1257 2037 455
Fragments arranged by size:
2037 1257 455
BsaHI Gr'CG_yC
Cuts at: 1180 1428 2130 3626 1180
Size: 248 702 1496 1303
Fragments arranged by size:
1496 1303 702 248
BsaWI w'CCGG_w
Cuts at: 938 1459 2137 2411 2558 2969 938
Size: 521 678 274 147 411 1718
Fragments arranged by size:
1718 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1174 1932 2509 3005 1174
Size: 758 577 496 1918
Fragments arranged by size:
1918 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1291 1337 2545 104
Size: 1187 46 1208 1308
Fragments arranged by size:
1308 1208 1187 46
BsiHKAI G_wGCw'C
Cuts at: 162 191 344 738 763 1541 1731 2523 162
Size: 29 153 394 25 778 190 792 1388
Fragments arranged by size:
1388 792 778 394 190 153 29 25
Bsp1286I G_dGCh'C
Cuts at: 162 191 344 738 763 1374 1467 1541 1731
Size: 29 153 394 25 611 93 74 190
Cuts at: 1731 1793 2523 162
Size: 62 730 1388
Fragments arranged by size:
1388 730 611 394 190 153 93 74
62 29 25
BspEI T'CCGG_A
Cuts at: 2969 2969
Size: 3749
BspHI T'CATG_A
Cuts at: 122 678 2925 122
Size: 556 2247 946
Fragments arranged by size:
2247 946 556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 799 1013 1315 1696 799
Size: 214 302 381 2852
Fragments arranged by size:
2852 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2041 2095 3579 3744 50
Size: 1991 54 1484 165 55
Fragments arranged by size:
1991 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1661 1661
Size: 3749
BsrFI r'CCGG_y
Cuts at: 251 1747 1928 2137 251
Size: 1496 181 209 1863
Fragments arranged by size:
1863 1496 209 181
BssSI C'ACGA_G
Cuts at: 2020 2378 2020
Size: 358 3391
BstAPI GCAn_nnn'nTGC
Cuts at: 1126 1126
Size: 3749
BstBI TT'CG_AA
Cuts at: 2110 2110
Size: 3749
BstYI r'GATC_y
Cuts at: 1 774 1241 1264 1599 1845 2857 2943 2955
Size: 773 467 23 335 246 1012 86 12
Cuts at: 2955 3301 1
Size: 346 449
Fragments arranged by size:
1012 773 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1699 1880 2043 1699
Size: 181 163 3405
Fragments arranged by size:
3405 181 163
BtsI GCAGTG_nn'
Cuts at: 992 992
Size: 3749
DraI TTT'AAA
Cuts at: 748 748
Size: 3749
DrdI GACnn_nn'nnGTC
Cuts at: 1455 2313 1455
Size: 858 2891
EaeI y'GGCC_r
Cuts at: 1334 1508 1899 1926 3271 1334
Size: 174 391 27 1345 1812
Fragments arranged by size:
1812 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1334 1334
Size: 3749
EarI CTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 238 1957 2267 2413 3559 238
Size: 1719 310 146 1146 428
Fragments arranged by size:
1719 1146 428 310 146
EcoICRI GAG'CTC
Cuts at: 160 189 761 160
Size: 29 572 3148
Fragments arranged by size:
3148 572 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 521 690 1574 2006 2753 3021 146
Size: 375 169 884 432 747 268 874
Fragments arranged by size:
884 874 747 432 375 268 169
EcoO109I rG'GnC_Cy
Cuts at: 888 888
Size: 3749
EcoRI G'AATT_C
Cuts at: 753 753
Size: 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 402 1768 402
Size: 1366 2383
FspI TGC'GCA
Cuts at: 1530 1530
Size: 3749
HaeII r_GCGC'y
Cuts at: 185 264 1183 1431 2453 3432 185
Size: 79 919 248 1022 979 502
Fragments arranged by size:
1022 979 919 502 248 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 117 149 1591 1621 1623 1653 117
Size: 32 1442 30 2 30 2213
Fragments arranged by size:
2213 1442 32 30 30 2
HincII GTy'rAC
Cuts at: 330 788 330
Size: 458 3291
HindIII A'AGCT_T
Cuts at: 556 556
Size: 3749
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 769 769
Size: 3749
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 623 2420 2604 623
Size: 1797 184 1768
Fragments arranged by size:
1797 1768 184
MscI TGG'CCA
Cuts at: 1510 1510
Size: 3749
MslI CAynn'nnrTG
Cuts at: 1738 1865 1738
Size: 127 3622
MspA1I CmG'CkG
Cuts at: 860 1174 1534 2547 2792 860
Size: 314 360 1013 245 1817
Fragments arranged by size:
1817 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1930 1930
Size: 3749
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NcoI C'CATG_G
Cuts at: 107 107
Size: 3749
NgoMIV G'CCGG_C
Cuts at: 1928 1928
Size: 3749
NheI G'CTAG_C
Cuts at: 442 442
Size: 3749
NspI r_CATG'y
Cuts at: 802 1302 2209 802
Size: 500 907 2342
Fragments arranged by size:
2342 907 500
PciI A'CATG_T
Cuts at: 2205 2205
Size: 3749
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2888 2920 2888
Size: 32 3717
PstI C_TGCA'G
Cuts at: 796 1481 796
Size: 685 3064
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 1174 1534 1174
Size: 360 3389
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 162 191 763 162
Size: 29 572 3148
Fragments arranged by size:
3148 572 29
SalI G'TCGA_C
Cuts at: 786 786
Size: 3749
SapI GCTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 3266 3266
Size: 3749
SexAI A'CCwGG_T
Cuts at: 637 637
Size: 3749
SfcI C'TryA_G
Cuts at: 33 792 1477 2470 2661 33
Size: 759 685 993 191 1121
Fragments arranged by size:
1121 993 759 685 191
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SmaI CCC'GGG
Cuts at: 771 771
Size: 3749
SmlI C'TyrA_G
Cuts at: 904 1819 2311 2573 904
Size: 915 492 262 2080
Fragments arranged by size:
2080 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3749
SphI G_CATG'C
Cuts at: 802 1302 802
Size: 500 3249
StyI C'CwwG_G
Cuts at: 107 153 370 386 883 1237 107
Size: 46 217 16 497 354 2619
Fragments arranged by size:
2619 497 354 217 46 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 190 1332 1465 1924 2133 190
Size: 1142 133 459 209 1806
Fragments arranged by size:
1806 1142 459 209 133
TatI w'GTAC_w
Cuts at: 3264 3264
Size: 3749
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 216 248 277 309 3266 3298 216
Size: 32 29 32 2957 32 667
Fragments arranged by size:
2957 667 32 32 32 29
Tth111I GACn'n_nGTC
Cuts at: 1546 1546
Size: 3749
XbaI T'CTAG_A
Cuts at: 780 780
Size: 3749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 384 1244 384
Size: 860 2889
XmaI C'CCGG_G
Cuts at: 769 769
Size: 3749
XmnI GAAnn'nnTTC
Cuts at: 612 612
Size: 3749
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BglI BmgBI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI