Restriction map for
pF1KB6956
(Circular) (Six-base) MAPSORT of: pF1KB6956.seq Check: 8750 from: 1 to: 3827
pF1KB6956 3827 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 399 399
Size: 3827
AatII G_ACGT'C
Cuts at: 402 402
Size: 3827
AccI GT'mk_AC
Cuts at: 865 865
Size: 3827
Acc65I G'GTAC_C
Cuts at: 843 843
Size: 3827
AclI AA'CG_TT
Cuts at: 3667 3667
Size: 3827
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1652 2084 2831 3099 1652
Size: 432 747 268 2380
Fragments arranged by size:
2380 747 432 268
AflIII A'CryG_T
Cuts at: 1179 2283 3260 1179
Size: 1104 977 1746
Fragments arranged by size:
1746 1104 977
AgeI A'CCGG_T
Cuts at: 2215 2215
Size: 3827
AlwNI CAG_nnn'CTG
Cuts at: 1132 2699 1132
Size: 1567 2260
ApaLI G'TGCA_C
Cuts at: 2597 2597
Size: 3827
ApoI r'AATT_y
Cuts at: 265 831 3180 3436 265
Size: 566 2349 256 656
Fragments arranged by size:
2349 656 566 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3827
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3827
AvaI C'yCGr_G
Cuts at: 332 847 332
Size: 515 3312
BamHI G'GATC_C
Cuts at: 852 852
Size: 3827
BanI G'GyrC_C
Cuts at: 843 1257 1505 1540 843
Size: 414 248 35 3130
Fragments arranged by size:
3130 414 248 35
BanII G_rGCy'C
Cuts at: 841 1871 841
Size: 1030 2797
BbeI G_GCGC'C
Cuts at: 1261 1509 1261
Size: 248 3579
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 342 376 342
Size: 34 3793
BciVI GTATCCnnnnn_n'
Cuts at: 1725 2486 3827 1725
Size: 761 1341 1725
Fragments arranged by size:
1725 1341 761
BclI T'GATC_A
Cuts at: 1347 1347
Size: 3827
BglII A'GATC_T
Cuts at: 598 640 748 1342 3379 598
Size: 42 108 594 2037 1046
Fragments arranged by size:
2037 1046 594 108 42
BlpI GC'TnA_GC
Cuts at: 939 939
Size: 3827
Bme1580I G_kGCm'C
Cuts at: 1452 1545 2601 1452
Size: 93 1056 2678
Fragments arranged by size:
2678 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1039 1120 1455 3599 1039
Size: 81 335 2144 1267
Fragments arranged by size:
2144 1267 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 260 2194 260
Size: 1934 1893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 230
Size: 3827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1882 2374 2672 1003
Size: 879 492 298 2158
Fragments arranged by size:
2158 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3531 3531
Size: 3827
BsaAI yAC'GTr
Cuts at: 1180 1180
Size: 3827
BsaBI GATnn'nnATC
Cuts at: 6 464 597 621 702 753 1341 3378 6
Size: 458 133 24 81 51 588 2037 455
Fragments arranged by size:
2037 588 458 455 133 81 51 24
BsaHI Gr'CG_yC
Cuts at: 399 1258 1506 2208 3704 399
Size: 859 248 702 1496 522
Fragments arranged by size:
1496 859 702 522 248
BsaWI w'CCGG_w
Cuts at: 1016 1537 2215 2489 2636 3047 1016
Size: 521 678 274 147 411 1796
Fragments arranged by size:
1796 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 360 390 360
Size: 30 3797
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 146 575 146
Size: 429 3398
BseYI C'CCAG_C
Cuts at: 1252 2010 2587 3083 1252
Size: 758 577 496 1996
Fragments arranged by size:
1996 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1369 1415 2623 104
Size: 1265 46 1208 1308
Fragments arranged by size:
1308 1265 1208 46
BsiHKAI G_wGCw'C
Cuts at: 841 1619 1809 2601 841
Size: 778 190 792 2067
Fragments arranged by size:
2067 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 841 1452 1545 1619 1809 1871 2601 841
Size: 611 93 74 190 62 730 2067
Fragments arranged by size:
2067 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3047 3047
Size: 3827
BspHI T'CATG_A
Cuts at: 3003 3003
Size: 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 399 482 877 1091 1393 1774 399
Size: 83 395 214 302 381 2452
Fragments arranged by size:
2452 395 381 302 214 83
BsrBI CCG'CTC
Cuts at: 50 2119 2173 3657 3822 50
Size: 2069 54 1484 165 55
Fragments arranged by size:
2069 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1739 1739
Size: 3827
BsrFI r'CCGG_y
Cuts at: 476 1825 2006 2215 476
Size: 1349 181 209 2088
Fragments arranged by size:
2088 1349 209 181
BssSI C'ACGA_G
Cuts at: 2098 2456 2098
Size: 358 3469
BstAPI GCAn_nnn'nTGC
Cuts at: 292 1204 292
Size: 912 2915
BstBI TT'CG_AA
Cuts at: 269 2188 269
Size: 1919 1908
BstEII G'GTnAC_C
Cuts at: 154 154
Size: 3827
BstYI r'GATC_y
Cuts at: 1 273 598 640 703 748 852 1319 1342
Size: 272 325 42 63 45 104 467 23
Cuts at: 1342 1677 1923 2935 3021 3033 3379 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 325 272 246
104 86 63 45 42 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1777 1958 2121 1777
Size: 181 163 3483
Fragments arranged by size:
3483 181 163
BtsI GCAGTG_nn'
Cuts at: 1070 1070
Size: 3827
DraI TTT'AAA
Cuts at: 826 826
Size: 3827
DrdI GACnn_nn'nnGTC
Cuts at: 1533 2391 1533
Size: 858 2969
EaeI y'GGCC_r
Cuts at: 1412 1586 1977 2004 3349 1412
Size: 174 391 27 1345 1890
Fragments arranged by size:
1890 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1412 1412
Size: 3827
EarI CTCTTCn'nnn_
Cuts at: 614 1850 2060 614
Size: 1236 210 2381
Fragments arranged by size:
2381 1236 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2035 2345 2491 3637 2035
Size: 310 146 1146 2225
Fragments arranged by size:
2225 1146 310 146
EcoICRI GAG'CTC
Cuts at: 839 839
Size: 3827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 1652 2084 2831 3099 230
Size: 1422 432 747 268 958
Fragments arranged by size:
1422 958 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 966 966
Size: 3827
EcoRI G'AATT_C
Cuts at: 265 831 265
Size: 566 3261
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 644 1846 644
Size: 1202 2625
FspI TGC'GCA
Cuts at: 1608 1608
Size: 3827
HaeII r_GCGC'y
Cuts at: 173 1261 1509 2531 3510 173
Size: 1088 248 1022 979 490
Fragments arranged by size:
1088 1022 979 490 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1669 1699 1701 1731 1669
Size: 30 2 30 3765
Fragments arranged by size:
3765 30 30 2
HincII GTy'rAC
Cuts at: 866 866
Size: 3827
KasI G'GCGC_C
Cuts at: 1257 1505 1257
Size: 248 3579
KpnI G_GTAC'C
Cuts at: 847 847
Size: 3827
MluI A'CGCG_T
Cuts at: 3260 3260
Size: 3827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2498 2682 2498
Size: 184 3643
MscI TGG'CCA
Cuts at: 1588 1588
Size: 3827
MslI CAynn'nnrTG
Cuts at: 1816 1943 1816
Size: 127 3700
MspA1I CmG'CkG
Cuts at: 938 1252 1612 2625 2870 938
Size: 314 360 1013 245 1895
Fragments arranged by size:
1895 1013 360 314 245
NaeI GCC'GGC
Cuts at: 478 2008 478
Size: 1530 2297
NarI GG'CG_CC
Cuts at: 1258 1506 1258
Size: 248 3579
NgoMIV G'CCGG_C
Cuts at: 476 2006 476
Size: 1530 2297
NspI r_CATG'y
Cuts at: 363 880 1380 2287 363
Size: 517 500 907 1903
Fragments arranged by size:
1903 907 517 500
PciI A'CATG_T
Cuts at: 2283 2283
Size: 3827
PfoI T'CCnGG_A
Cuts at: 246 246
Size: 3827
PmeI GTTT'AAAC
Cuts at: 826 826
Size: 3827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2966 2998 2966
Size: 32 3795
PshAI GACnn'nnGTC
Cuts at: 567 567
Size: 3827
PstI C_TGCA'G
Cuts at: 874 1559 874
Size: 685 3142
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3827
PvuII CAG'CTG
Cuts at: 1252 1612 1252
Size: 360 3467
RsrII CG'GwC_CG
Cuts at: 2022 2022
Size: 3827
SacI G_AGCT'C
Cuts at: 841 841
Size: 3827
SalI G'TCGA_C
Cuts at: 864 864
Size: 3827
SapI GCTCTTCn'nnn_
Cuts at: 1850 2060 1850
Size: 210 3617
SbfI CC_TGCA'GG
Cuts at: 874 874
Size: 3827
ScaI AGT'ACT
Cuts at: 3344 3344
Size: 3827
SfcI C'TryA_G
Cuts at: 33 870 1555 2548 2739 33
Size: 837 685 993 191 1121
Fragments arranged by size:
1121 993 837 685 191
SfoI GGC'GCC
Cuts at: 1259 1507 1259
Size: 248 3579
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3827
SmaI CCC'GGG
Cuts at: 849 849
Size: 3827
SmlI C'TyrA_G
Cuts at: 982 1897 2389 2651 982
Size: 915 492 262 2158
Fragments arranged by size:
2158 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3827
SphI G_CATG'C
Cuts at: 363 880 1380 363
Size: 517 500 2810
Fragments arranged by size:
2810 517 500
StyI C'CwwG_G
Cuts at: 140 961 1315 140
Size: 821 354 2652
Fragments arranged by size:
2652 821 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1410 1543 2002 2211 1410
Size: 133 459 209 3026
Fragments arranged by size:
3026 459 209 133
TatI w'GTAC_w
Cuts at: 3342 3342
Size: 3827
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3344 3376 3344
Size: 32 3795
Tth111I GACn'n_nGTC
Cuts at: 1624 1624
Size: 3827
XbaI T'CTAG_A
Cuts at: 502 858 502
Size: 356 3471
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1322 1322
Size: 3827
XmaI C'CCGG_G
Cuts at: 847 847
Size: 3827
XmnI GAAnn'nnTTC
Cuts at: 771 771
Size: 3827
ZraI GAC'GTC
Cuts at: 400 400
Size: 3827
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI