Restriction map for
pF1KB6957
(Circular) (Six-base) MAPSORT of: pF1KB6957.seq Check: 3552 from: 1 to: 3752
pF1KB6957 3752 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 790 790
Size: 3752
Acc65I G'GTAC_C
Cuts at: 565 768 565
Size: 203 3549
AclI AA'CG_TT
Cuts at: 3592 3592
Size: 3752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1577 2009 2756 3024 1577
Size: 432 747 268 2305
Fragments arranged by size:
2305 747 432 268
AfeI AGC'GCT
Cuts at: 175 175
Size: 3752
AflIII A'CryG_T
Cuts at: 1104 2208 3185 1104
Size: 1104 977 1671
Fragments arranged by size:
1671 1104 977
AgeI A'CCGG_T
Cuts at: 2140 2140
Size: 3752
AleI CACnn'nnGTG
Cuts at: 507 507
Size: 3752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 334 334
Size: 3752
AlwNI CAG_nnn'CTG
Cuts at: 353 1057 2624 353
Size: 704 1567 1481
Fragments arranged by size:
1567 1481 704
ApaI G_GGCC'C
Cuts at: 255 262 255
Size: 7 3745
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 756 3105 3361 756
Size: 2349 256 1147
Fragments arranged by size:
2349 1147 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 359 655 772 359
Size: 296 117 3339
Fragments arranged by size:
3339 296 117
BamHI G'GATC_C
Cuts at: 777 777
Size: 3752
BanI G'GyrC_C
Cuts at: 565 768 1182 1430 1465 565
Size: 203 414 248 35 2852
Fragments arranged by size:
2852 414 248 203 35
BanII G_rGCy'C
Cuts at: 255 262 270 485 766 1796 255
Size: 7 8 215 281 1030 2211
Fragments arranged by size:
2211 1030 281 215 8 7
BbeI G_GCGC'C
Cuts at: 1186 1434 1186
Size: 248 3504
BbsI GAAGACnn'nnnn_
Cuts at: 727 727
Size: 3752
BciVI GTATCCnnnnn_n'
Cuts at: 1650 2411 3752 1650
Size: 761 1341 1650
Fragments arranged by size:
1650 1341 761
BclI T'GATC_A
Cuts at: 1272 1272
Size: 3752
BglI GCCn_nnn'nGGC
Cuts at: 131 308 496 131
Size: 177 188 3387
Fragments arranged by size:
3387 188 177
BglII A'GATC_T
Cuts at: 1267 3304 1267
Size: 2037 1715
BlpI GC'TnA_GC
Cuts at: 864 864
Size: 3752
Bme1580I G_kGCm'C
Cuts at: 255 262 1377 1470 2526 255
Size: 7 1115 93 1056 1481
Fragments arranged by size:
1481 1115 1056 93 7
BmrI ACTGGGnnnn_n'
Cuts at: 964 1045 1380 3524 964
Size: 81 335 2144 1192
Fragments arranged by size:
2144 1192 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 3752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 293 307 284
Size: 9 14 3729
Fragments arranged by size:
3729 14 9
Bpu10I CC'TnA_GC
Cuts at: 643 643
Size: 3752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 928 1807 2299 2597 157
Size: 771 879 492 298 1312
Fragments arranged by size:
1312 879 771 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3456 3456
Size: 3752
BsaAI yAC'GTr
Cuts at: 1105 1105
Size: 3752
BsaBI GATnn'nnATC
Cuts at: 6 1266 3303 6
Size: 1260 2037 455
Fragments arranged by size:
2037 1260 455
BsaHI Gr'CG_yC
Cuts at: 652 1183 1431 2133 3629 652
Size: 531 248 702 1496 775
Fragments arranged by size:
1496 775 702 531 248
BsaWI w'CCGG_w
Cuts at: 941 1462 2140 2414 2561 2972 941
Size: 521 678 274 147 411 1721
Fragments arranged by size:
1721 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 312 493 312
Size: 181 3571
BseYI C'CCAG_C
Cuts at: 192 413 1177 1935 2512 3008 192
Size: 221 764 758 577 496 936
Fragments arranged by size:
936 764 758 577 496 221
BsiEI CG_ry'CG
Cuts at: 104 1294 1340 2548 104
Size: 1190 46 1208 1308
Fragments arranged by size:
1308 1208 1190 46
BsiHKAI G_wGCw'C
Cuts at: 485 712 766 1544 1734 2526 485
Size: 227 54 778 190 792 1711
Fragments arranged by size:
1711 792 778 227 190 54
Bsp1286I G_dGCh'C
Cuts at: 255 262 270 485 712 766 1377 1470 1544
Size: 7 8 215 227 54 611 93 74
Cuts at: 1544 1734 1796 2526 255
Size: 190 62 730 1481
Fragments arranged by size:
1481 730 611 227 215 190 93 74
62 54 8 7
BspEI T'CCGG_A
Cuts at: 2972 2972
Size: 3752
BspHI T'CATG_A
Cuts at: 515 2928 515
Size: 2413 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 302 802 1016 1318 1699 302
Size: 500 214 302 381 2355
Fragments arranged by size:
2355 500 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2044 2098 3582 3747 50
Size: 1994 54 1484 165 55
Fragments arranged by size:
1994 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1664 1664
Size: 3752
BsrFI r'CCGG_y
Cuts at: 284 302 1750 1931 2140 284
Size: 18 1448 181 209 1896
Fragments arranged by size:
1896 1448 209 181 18
BssSI C'ACGA_G
Cuts at: 2023 2381 2023
Size: 358 3394
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 1129
Size: 3752
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstEII G'GTnAC_C
Cuts at: 207 546 207
Size: 339 3413
BstYI r'GATC_y
Cuts at: 1 777 1244 1267 1602 1848 2860 2946 2958
Size: 776 467 23 335 246 1012 86 12
Cuts at: 2958 3304 1
Size: 346 449
Fragments arranged by size:
1012 776 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 166 295 166
Size: 129 3623
BtgI C'CryG_G
Cuts at: 203 213 713 203
Size: 10 500 3242
Fragments arranged by size:
3242 500 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 224 1702 1883 2046 224
Size: 1478 181 163 1930
Fragments arranged by size:
1930 1478 181 163
BtsI GCAGTG_nn'
Cuts at: 667 995 667
Size: 328 3424
DraI TTT'AAA
Cuts at: 751 751
Size: 3752
DrdI GACnn_nn'nnGTC
Cuts at: 1458 2316 1458
Size: 858 2894
EaeI y'GGCC_r
Cuts at: 200 629 1337 1511 1902 1929 3274 200
Size: 429 708 174 391 27 1345 678
Fragments arranged by size:
1345 708 678 429 391 174 27
EagI C'GGCC_G
Cuts at: 1337 1337
Size: 3752
EarI CTCTTCn'nnn_
Cuts at: 1775 1985 1775
Size: 210 3542
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1960 2270 2416 3562 1960
Size: 310 146 1146 2150
Fragments arranged by size:
2150 1146 310 146
EcoICRI GAG'CTC
Cuts at: 483 764 483
Size: 281 3471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 293 307 1577 2009 2756 3024 284
Size: 9 14 1270 432 747 268 1012
Fragments arranged by size:
1270 1012 747 432 268 14 9
EcoO109I rG'GnC_Cy
Cuts at: 132 141 157 251 252 259 410 668 891
Size: 9 16 94 1 7 151 258 223
Cuts at: 891 132
Size: 2993
Fragments arranged by size:
2993 258 223 151 94 16 9 7
1
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1771 1771
Size: 3752
FspI TGC'GCA
Cuts at: 1533 1533
Size: 3752
HaeII r_GCGC'y
Cuts at: 177 1186 1434 2456 3435 177
Size: 1009 248 1022 979 494
Fragments arranged by size:
1022 1009 979 494 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1594 1624 1626 1656 1594
Size: 30 2 30 3690
Fragments arranged by size:
3690 30 30 2
HincII GTy'rAC
Cuts at: 791 791
Size: 3752
KasI G'GCGC_C
Cuts at: 1182 1430 1182
Size: 248 3504
KpnI G_GTAC'C
Cuts at: 569 772 569
Size: 203 3549
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2607 2423
Size: 184 3568
MscI TGG'CCA
Cuts at: 1513 1513
Size: 3752
MslI CAynn'nnrTG
Cuts at: 507 1741 1868 507
Size: 1234 127 2391
Fragments arranged by size:
2391 1234 127
MspA1I CmG'CkG
Cuts at: 235 407 686 863 1177 1537 2550 2795 235
Size: 172 279 177 314 360 1013 245 1192
Fragments arranged by size:
1192 1013 360 314 279 245 177 172
NaeI GCC'GGC
Cuts at: 1933 1933
Size: 3752
NarI GG'CG_CC
Cuts at: 1183 1431 1183
Size: 248 3504
NcoI C'CATG_G
Cuts at: 213 213
Size: 3752
NgoMIV G'CCGG_C
Cuts at: 1931 1931
Size: 3752
NspI r_CATG'y
Cuts at: 397 805 1305 2212 397
Size: 408 500 907 1937
Fragments arranged by size:
1937 907 500 408
PasI CC'CwG_GG
Cuts at: 255 579 255
Size: 324 3428
PciI A'CATG_T
Cuts at: 2208 2208
Size: 3752
PflMI CCAn_nnn'nTGG
Cuts at: 353 585 353
Size: 232 3520
PfoI T'CCnGG_A
Cuts at: 334 531 334
Size: 197 3555
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PpuMI rG'GwC_Cy
Cuts at: 410 668 410
Size: 258 3494
PspOMI G'GGCC_C
Cuts at: 251 258 251
Size: 7 3745
PstI C_TGCA'G
Cuts at: 738 799 1484 738
Size: 61 685 3006
Fragments arranged by size:
3006 685 61
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3752
PvuII CAG'CTG
Cuts at: 235 686 1177 1537 235
Size: 451 491 360 2450
Fragments arranged by size:
2450 491 451 360
RsrII CG'GwC_CG
Cuts at: 1947 1947
Size: 3752
SacI G_AGCT'C
Cuts at: 485 766 485
Size: 281 3471
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SanDI GG'GwC_CC
Cuts at: 410 410
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 1775 1985 1775
Size: 210 3542
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 3269 3269
Size: 3752
SfcI C'TryA_G
Cuts at: 33 681 734 795 1480 2473 2664 33
Size: 648 53 61 685 993 191 1121
Fragments arranged by size:
1121 993 685 648 191 61 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 131 131
Size: 3752
SfoI GGC'GCC
Cuts at: 1184 1432 1184
Size: 248 3504
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SmaI CCC'GGG
Cuts at: 361 774 361
Size: 413 3339
SmlI C'TyrA_G
Cuts at: 136 907 1822 2314 2576 136
Size: 771 915 492 262 1312
Fragments arranged by size:
1312 915 771 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
StyI C'CwwG_G
Cuts at: 213 437 886 1240 213
Size: 224 449 354 2725
Fragments arranged by size:
2725 449 354 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 441 592 1335 1468 1927 2136 441
Size: 151 743 133 459 209 2057
Fragments arranged by size:
2057 743 459 209 151 133
TatI w'GTAC_w
Cuts at: 3267 3267
Size: 3752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 3720
Tth111I GACn'n_nGTC
Cuts at: 1549 1549
Size: 3752
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1247 1247
Size: 3752
XmaI C'CCGG_G
Cuts at: 359 772 359
Size: 413 3339
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI