Restriction map for
pF1KB6961
(Circular) (Six-base) MAPSORT of: pF1KB6961.seq Check: 5810 from: 1 to: 3638
pF1KB6961 3638 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 676 676
Size: 3638
Acc65I G'GTAC_C
Cuts at: 654 654
Size: 3638
AclI AA'CG_TT
Cuts at: 3478 3478
Size: 3638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 1895 2642 2910 1463
Size: 432 747 268 2191
Fragments arranged by size:
2191 747 432 268
AflIII A'CryG_T
Cuts at: 990 2094 3071 990
Size: 1104 977 1557
Fragments arranged by size:
1557 1104 977
AgeI A'CCGG_T
Cuts at: 2026 2026
Size: 3638
AlwNI CAG_nnn'CTG
Cuts at: 943 2510 943
Size: 1567 2071
ApaLI G'TGCA_C
Cuts at: 2408 2408
Size: 3638
ApoI r'AATT_y
Cuts at: 391 642 2991 3247 391
Size: 251 2349 256 782
Fragments arranged by size:
2349 782 256 251
AseI AT'TA_AT
Cuts at: 20 20
Size: 3638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
AvaI C'yCGr_G
Cuts at: 658 658
Size: 3638
BamHI G'GATC_C
Cuts at: 663 663
Size: 3638
BanI G'GyrC_C
Cuts at: 214 654 1068 1316 1351 214
Size: 440 414 248 35 2501
Fragments arranged by size:
2501 440 414 248 35
BanII G_rGCy'C
Cuts at: 652 1682 652
Size: 1030 2608
BbeI G_GCGC'C
Cuts at: 1072 1320 1072
Size: 248 3390
BbsI GAAGACnn'nnnn_
Cuts at: 325 325
Size: 3638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3604
BciVI GTATCCnnnnn_n'
Cuts at: 1536 2297 3638 1536
Size: 761 1341 1536
Fragments arranged by size:
1536 1341 761
BclI T'GATC_A
Cuts at: 1158 1158
Size: 3638
BglII A'GATC_T
Cuts at: 1153 3190 1153
Size: 2037 1601
BlpI GC'TnA_GC
Cuts at: 750 750
Size: 3638
Bme1580I G_kGCm'C
Cuts at: 1263 1356 2412 1263
Size: 93 1056 2489
Fragments arranged by size:
2489 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 316 850 931 1266 3410 316
Size: 534 81 335 2144 544
Fragments arranged by size:
2144 544 534 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 464 814 1693 2185 2483 213
Size: 251 350 879 492 298 1368
Fragments arranged by size:
1368 879 492 350 298 251
BsaI GGTCTCn'nnnn_
Cuts at: 3342 3342
Size: 3638
BsaAI yAC'GTr
Cuts at: 991 991
Size: 3638
BsaBI GATnn'nnATC
Cuts at: 6 1152 3189 6
Size: 1146 2037 455
Fragments arranged by size:
2037 1146 455
BsaHI Gr'CG_yC
Cuts at: 1069 1317 2019 3515 1069
Size: 248 702 1496 1192
Fragments arranged by size:
1496 1192 702 248
BsaWI w'CCGG_w
Cuts at: 827 1348 2026 2300 2447 2858 827
Size: 521 678 274 147 411 1607
Fragments arranged by size:
1607 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1063 1821 2398 2894 1063
Size: 758 577 496 1807
Fragments arranged by size:
1807 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1180 1226 2434 104
Size: 1076 46 1208 1308
Fragments arranged by size:
1308 1208 1076 46
BsiHKAI G_wGCw'C
Cuts at: 652 1430 1620 2412 652
Size: 778 190 792 1878
Fragments arranged by size:
1878 792 778 190
BsmI GAATG_Cn'
Cuts at: 148 148
Size: 3638
Bsp1286I G_dGCh'C
Cuts at: 652 1263 1356 1430 1620 1682 2412 652
Size: 611 93 74 190 62 730 1878
Fragments arranged by size:
1878 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2858 2858
Size: 3638
BspHI T'CATG_A
Cuts at: 2814 2814
Size: 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 688 902 1204 1585 688
Size: 214 302 381 2741
Fragments arranged by size:
2741 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1930 1984 3468 3633 50
Size: 1880 54 1484 165 55
Fragments arranged by size:
1880 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1550 1550
Size: 3638
BsrFI r'CCGG_y
Cuts at: 1636 1817 2026 1636
Size: 181 209 3248
Fragments arranged by size:
3248 209 181
BssSI C'ACGA_G
Cuts at: 1909 2267 1909
Size: 358 3280
BstAPI GCAn_nnn'nTGC
Cuts at: 1015 1015
Size: 3638
BstBI TT'CG_AA
Cuts at: 1999 1999
Size: 3638
BstEII G'GTnAC_C
Cuts at: 150 150
Size: 3638
BstYI r'GATC_y
Cuts at: 1 663 1130 1153 1488 1734 2746 2832 2844
Size: 662 467 23 335 246 1012 86 12
Cuts at: 2844 3190 1
Size: 346 449
Fragments arranged by size:
1012 662 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 206 206
Size: 3638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1588 1769 1932 1588
Size: 181 163 3294
Fragments arranged by size:
3294 181 163
BtsI GCAGTG_nn'
Cuts at: 881 881
Size: 3638
ClaI AT'CG_AT
Cuts at: 221 221
Size: 3638
DraI TTT'AAA
Cuts at: 168 637 168
Size: 469 3169
DrdI GACnn_nn'nnGTC
Cuts at: 1344 2202 1344
Size: 858 2780
EaeI y'GGCC_r
Cuts at: 1223 1397 1788 1815 3160 1223
Size: 174 391 27 1345 1701
Fragments arranged by size:
1701 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1223 1223
Size: 3638
EarI CTCTTCn'nnn_
Cuts at: 434 567 1661 1871 434
Size: 133 1094 210 2201
Fragments arranged by size:
2201 1094 210 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1846 2156 2302 3448 1846
Size: 310 146 1146 2036
Fragments arranged by size:
2036 1146 310 146
EcoICRI GAG'CTC
Cuts at: 650 650
Size: 3638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 1895 2642 2910 1463
Size: 432 747 268 2191
Fragments arranged by size:
2191 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 777 777
Size: 3638
EcoRI G'AATT_C
Cuts at: 642 642
Size: 3638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1657 1657
Size: 3638
FspI TGC'GCA
Cuts at: 1419 1419
Size: 3638
HaeII r_GCGC'y
Cuts at: 1072 1320 2342 3321 1072
Size: 248 1022 979 1389
Fragments arranged by size:
1389 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 240 272 312 344 1480 1510 1512 1542 240
Size: 32 40 32 1136 30 2 30 2336
Fragments arranged by size:
2336 1136 40 32 32 30 30 2
HincII GTy'rAC
Cuts at: 116 617 677 116
Size: 501 60 3077
Fragments arranged by size:
3077 501 60
HpaI GTT'AAC
Cuts at: 116 116
Size: 3638
KasI G'GCGC_C
Cuts at: 1068 1316 1068
Size: 248 3390
KpnI G_GTAC'C
Cuts at: 658 658
Size: 3638
MluI A'CGCG_T
Cuts at: 3071 3071
Size: 3638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2309 2493 2309
Size: 184 3454
MscI TGG'CCA
Cuts at: 1399 1399
Size: 3638
MslI CAynn'nnrTG
Cuts at: 1627 1754 1627
Size: 127 3511
MspA1I CmG'CkG
Cuts at: 126 749 1063 1423 2436 2681 126
Size: 623 314 360 1013 245 1083
Fragments arranged by size:
1083 1013 623 360 314 245
NaeI GCC'GGC
Cuts at: 1819 1819
Size: 3638
NarI GG'CG_CC
Cuts at: 1069 1317 1069
Size: 248 3390
NcoI C'CATG_G
Cuts at: 206 206
Size: 3638
NgoMIV G'CCGG_C
Cuts at: 1817 1817
Size: 3638
NspI r_CATG'y
Cuts at: 691 1191 2098 691
Size: 500 907 2231
Fragments arranged by size:
2231 907 500
PciI A'CATG_T
Cuts at: 2094 2094
Size: 3638
PmeI GTTT'AAAC
Cuts at: 637 637
Size: 3638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 2777
Size: 32 3606
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 318 350 318
Size: 32 3606
PstI C_TGCA'G
Cuts at: 685 1370 685
Size: 685 2953
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3638
PvuII CAG'CTG
Cuts at: 126 1063 1423 126
Size: 937 360 2341
Fragments arranged by size:
2341 937 360
RsrII CG'GwC_CG
Cuts at: 1833 1833
Size: 3638
SacI G_AGCT'C
Cuts at: 652 652
Size: 3638
SalI G'TCGA_C
Cuts at: 675 675
Size: 3638
SapI GCTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
SbfI CC_TGCA'GG
Cuts at: 685 685
Size: 3638
ScaI AGT'ACT
Cuts at: 3155 3155
Size: 3638
SfcI C'TryA_G
Cuts at: 33 242 681 1366 2359 2550 33
Size: 209 439 685 993 191 1121
Fragments arranged by size:
1121 993 685 439 209 191
SfoI GGC'GCC
Cuts at: 1070 1318 1070
Size: 248 3390
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
SmaI CCC'GGG
Cuts at: 660 660
Size: 3638
SmlI C'TyrA_G
Cuts at: 192 479 793 1708 2200 2462 192
Size: 287 314 915 492 262 1368
Fragments arranged by size:
1368 915 492 314 287 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3638
SphI G_CATG'C
Cuts at: 691 1191 691
Size: 500 3138
SspI AAT'ATT
Cuts at: 372 531 372
Size: 159 3479
StyI C'CwwG_G
Cuts at: 206 772 1126 206
Size: 566 354 2718
Fragments arranged by size:
2718 566 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1221 1354 1813 2022 1221
Size: 133 459 209 2837
Fragments arranged by size:
2837 459 209 133
TatI w'GTAC_w
Cuts at: 3153 3153
Size: 3638
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3606
Tth111I GACn'n_nGTC
Cuts at: 1435 1435
Size: 3638
XbaI T'CTAG_A
Cuts at: 669 669
Size: 3638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1133 1133
Size: 3638
XmaI C'CCGG_G
Cuts at: 658 658
Size: 3638
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI