Restriction map for
pF1KB6962
(Circular) (Six-base) MAPSORT of: pF1KB6962.seq Check: 1165 from: 1 to: 3833
pF1KB6962 3833 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 326 600 641 871 326
Size: 274 41 230 3288
Fragments arranged by size:
3288 274 230 41
Acc65I G'GTAC_C
Cuts at: 849 849
Size: 3833
AclI AA'CG_TT
Cuts at: 3673 3673
Size: 3833
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1658 2090 2837 3105 1658
Size: 432 747 268 2386
Fragments arranged by size:
2386 747 432 268
AflIII A'CryG_T
Cuts at: 1185 2289 3266 1185
Size: 1104 977 1752
Fragments arranged by size:
1752 1104 977
AgeI A'CCGG_T
Cuts at: 2221 2221
Size: 3833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 787 787
Size: 3833
AlwNI CAG_nnn'CTG
Cuts at: 484 1138 2705 484
Size: 654 1567 1612
Fragments arranged by size:
1612 1567 654
ApaI G_GGCC'C
Cuts at: 592 592
Size: 3833
ApaLI G'TGCA_C
Cuts at: 2603 2603
Size: 3833
ApoI r'AATT_y
Cuts at: 837 3186 3442 837
Size: 2349 256 1228
Fragments arranged by size:
2349 1228 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3833
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
AvaI C'yCGr_G
Cuts at: 853 853
Size: 3833
BamHI G'GATC_C
Cuts at: 858 858
Size: 3833
BanI G'GyrC_C
Cuts at: 178 191 381 532 849 1263 1511 1546 178
Size: 13 190 151 317 414 248 35 2465
Fragments arranged by size:
2465 414 317 248 190 151 35 13
BanII G_rGCy'C
Cuts at: 592 654 847 1877 592
Size: 62 193 1030 2548
Fragments arranged by size:
2548 1030 193 62
BbeI G_GCGC'C
Cuts at: 182 385 1267 1515 182
Size: 203 882 248 2500
Fragments arranged by size:
2500 882 248 203
BbvCI CC'TCA_GC
Cuts at: 287 287
Size: 3833
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 398 432 722 756 398
Size: 34 290 34 3475
Fragments arranged by size:
3475 290 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1731 2492 3833 1731
Size: 761 1341 1731
Fragments arranged by size:
1731 1341 761
BclI T'GATC_A
Cuts at: 1353 1353
Size: 3833
BglI GCCn_nnn'nGGC
Cuts at: 176 176
Size: 3833
BglII A'GATC_T
Cuts at: 1348 3385 1348
Size: 2037 1796
BlpI GC'TnA_GC
Cuts at: 945 945
Size: 3833
Bme1580I G_kGCm'C
Cuts at: 196 592 671 1458 1551 2607 196
Size: 396 79 787 93 1056 1422
Fragments arranged by size:
1422 1056 787 396 93 79
BmrI ACTGGGnnnn_n'
Cuts at: 824 1045 1126 1461 3605 824
Size: 221 81 335 2144 1052
Fragments arranged by size:
2144 1052 335 221 81
BmtI G_CTAG'C
Cuts at: 151 151
Size: 3833
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 3833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 767 613
Size: 154 3679
Bpu10I CC'TnA_GC
Cuts at: 287 287
Size: 3833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1888 2380 2678 1009
Size: 879 492 298 2164
Fragments arranged by size:
2164 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 506 785 3537 506
Size: 279 2752 802
Fragments arranged by size:
2752 802 279
BsaAI yAC'GTr
Cuts at: 666 1186 666
Size: 520 3313
BsaBI GATnn'nnATC
Cuts at: 6 1347 3384 6
Size: 1341 2037 455
Fragments arranged by size:
2037 1341 455
BsaHI Gr'CG_yC
Cuts at: 179 382 1264 1512 2214 3710 179
Size: 203 882 248 702 1496 302
Fragments arranged by size:
1496 882 702 302 248 203
BsaWI w'CCGG_w
Cuts at: 695 1022 1543 2221 2495 2642 3053 695
Size: 327 521 678 274 147 411 1475
Fragments arranged by size:
1475 678 521 411 327 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 262 463 705 792 259
Size: 3 201 242 87 3300
Fragments arranged by size:
3300 242 201 87 3
BseYI C'CCAG_C
Cuts at: 551 1258 2016 2593 3089 551
Size: 707 758 577 496 1295
Fragments arranged by size:
1295 758 707 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 235
Size: 3833
BsiEI CG_ry'CG
Cuts at: 104 408 1375 1421 2629 104
Size: 304 967 46 1208 1308
Fragments arranged by size:
1308 1208 967 304 46
BsiHKAI G_wGCw'C
Cuts at: 847 1625 1815 2607 847
Size: 778 190 792 2073
Fragments arranged by size:
2073 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 196 592 654 671 847 1458 1551 1625 1815
Size: 396 62 17 176 611 93 74 190
Cuts at: 1815 1877 2607 196
Size: 62 730 1422
Fragments arranged by size:
1422 730 611 396 190 176 93 74
62 62 17
BspEI T'CCGG_A
Cuts at: 3053 3053
Size: 3833
BspHI T'CATG_A
Cuts at: 3009 3009
Size: 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 883 1097 1399 1780 883
Size: 214 302 381 2936
Fragments arranged by size:
2936 381 302 214
BsrBI CCG'CTC
Cuts at: 50 713 2125 2179 3663 3828 50
Size: 663 1412 54 1484 165 55
Fragments arranged by size:
1484 1412 663 165 55 54
BsrDI GCAATG_nn'
Cuts at: 798 1745 798
Size: 947 2886
BsrFI r'CCGG_y
Cuts at: 1831 2012 2221 1831
Size: 181 209 3443
Fragments arranged by size:
3443 209 181
BssHII G'CGCG_C
Cuts at: 431 431
Size: 3833
BssSI C'ACGA_G
Cuts at: 2104 2462 2104
Size: 358 3475
BstAPI GCAn_nnn'nTGC
Cuts at: 213 484 1210 213
Size: 271 726 2836
Fragments arranged by size:
2836 726 271
BstBI TT'CG_AA
Cuts at: 2194 2194
Size: 3833
BstYI r'GATC_y
Cuts at: 1 858 1325 1348 1683 1929 2941 3027 3039
Size: 857 467 23 335 246 1012 86 12
Cuts at: 3039 3385 1
Size: 346 449
Fragments arranged by size:
1012 857 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 458 458
Size: 3833
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1783 1964 2127 1783
Size: 181 163 3489
Fragments arranged by size:
3489 181 163
BtsI GCAGTG_nn'
Cuts at: 361 482 712 1076 361
Size: 121 230 364 3118
Fragments arranged by size:
3118 364 230 121
DraI TTT'AAA
Cuts at: 832 832
Size: 3833
DrdI GACnn_nn'nnGTC
Cuts at: 1539 2397 1539
Size: 858 2975
EaeI y'GGCC_r
Cuts at: 405 1418 1592 1983 2010 3355 405
Size: 1013 174 391 27 1345 883
Fragments arranged by size:
1345 1013 883 391 174 27
EagI C'GGCC_G
Cuts at: 405 1418 405
Size: 1013 2820
EarI CTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 353 2041 2351 2497 3643 353
Size: 1688 310 146 1146 543
Fragments arranged by size:
1688 1146 543 310 146
EcoICRI GAG'CTC
Cuts at: 845 845
Size: 3833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 767 1658 2090 2837 3105 613
Size: 154 891 432 747 268 1341
Fragments arranged by size:
1341 891 747 432 268 154
EcoO109I rG'GnC_Cy
Cuts at: 736 972 736
Size: 236 3597
EcoRI G'AATT_C
Cuts at: 837 837
Size: 3833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1852 1852
Size: 3833
FspI TGC'GCA
Cuts at: 1614 1614
Size: 3833
HaeII r_GCGC'y
Cuts at: 182 385 397 732 1267 1515 2537 3516 182
Size: 203 12 335 535 248 1022 979 499
Fragments arranged by size:
1022 979 535 499 335 248 203 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 244 276 1675 1705 1707 1737 244
Size: 32 1399 30 2 30 2340
Fragments arranged by size:
2340 1399 32 30 30 2
HincII GTy'rAC
Cuts at: 872 872
Size: 3833
KasI G'GCGC_C
Cuts at: 178 381 1263 1511 178
Size: 203 882 248 2500
Fragments arranged by size:
2500 882 248 203
KpnI G_GTAC'C
Cuts at: 853 853
Size: 3833
MfeI C'AATT_G
Cuts at: 672 672
Size: 3833
MluI A'CGCG_T
Cuts at: 3266 3266
Size: 3833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2504 2688 2504
Size: 184 3649
MscI TGG'CCA
Cuts at: 1594 1594
Size: 3833
MslI CAynn'nnrTG
Cuts at: 583 1822 1949 583
Size: 1239 127 2467
Fragments arranged by size:
2467 1239 127
MspA1I CmG'CkG
Cuts at: 135 416 944 1258 1618 2631 2876 135
Size: 281 528 314 360 1013 245 1092
Fragments arranged by size:
1092 1013 528 360 314 281 245
NaeI GCC'GGC
Cuts at: 2014 2014
Size: 3833
NarI GG'CG_CC
Cuts at: 179 382 1264 1512 179
Size: 203 882 248 2500
Fragments arranged by size:
2500 882 248 203
NgoMIV G'CCGG_C
Cuts at: 2012 2012
Size: 3833
NheI G'CTAG_C
Cuts at: 147 147
Size: 3833
NspI r_CATG'y
Cuts at: 886 1386 2293 886
Size: 500 907 2426
Fragments arranged by size:
2426 907 500
PasI CC'CwG_GG
Cuts at: 720 720
Size: 3833
PciI A'CATG_T
Cuts at: 2289 2289
Size: 3833
PmeI GTTT'AAAC
Cuts at: 832 832
Size: 3833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2972 3004 2972
Size: 32 3801
PpuMI rG'GwC_Cy
Cuts at: 736 736
Size: 3833
PshAI GACnn'nnGTC
Cuts at: 637 637
Size: 3833
PspOMI G'GGCC_C
Cuts at: 588 588
Size: 3833
PstI C_TGCA'G
Cuts at: 356 623 880 1565 356
Size: 267 257 685 2624
Fragments arranged by size:
2624 685 267 257
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3833
PvuII CAG'CTG
Cuts at: 1258 1618 1258
Size: 360 3473
RsrII CG'GwC_CG
Cuts at: 2028 2028
Size: 3833
SacI G_AGCT'C
Cuts at: 847 847
Size: 3833
SalI G'TCGA_C
Cuts at: 870 870
Size: 3833
SapI GCTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
SbfI CC_TGCA'GG
Cuts at: 880 880
Size: 3833
ScaI AGT'ACT
Cuts at: 3350 3350
Size: 3833
SfcI C'TryA_G
Cuts at: 33 352 619 876 1561 2554 2745 33
Size: 319 267 257 685 993 191 1121
Fragments arranged by size:
1121 993 685 319 267 257 191
SfoI GGC'GCC
Cuts at: 180 383 1265 1513 180
Size: 203 882 248 2500
Fragments arranged by size:
2500 882 248 203
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
SmaI CCC'GGG
Cuts at: 855 855
Size: 3833
SmlI C'TyrA_G
Cuts at: 988 1903 2395 2657 988
Size: 915 492 262 2164
Fragments arranged by size:
2164 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3833
SphI G_CATG'C
Cuts at: 886 1386 886
Size: 500 3333
StyI C'CwwG_G
Cuts at: 182 368 967 1321 182
Size: 186 599 354 2694
Fragments arranged by size:
2694 599 354 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1416 1549 2008 2217 1416
Size: 133 459 209 3032
Fragments arranged by size:
3032 459 209 133
TatI w'GTAC_w
Cuts at: 3348 3348
Size: 3833
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 3801
Tth111I GACn'n_nGTC
Cuts at: 1630 1630
Size: 3833
XbaI T'CTAG_A
Cuts at: 864 864
Size: 3833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1328 1328
Size: 3833
XmaI C'CCGG_G
Cuts at: 853 853
Size: 3833
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PasI PciI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BfrBI BmgBI BsaXI BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NcoI
NdeI NotI NruI NsiI PacI PflMI PfoI PmlI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI