Restriction map for
pF1KB6965
(Circular) (Six-base) MAPSORT of: pF1KB6965.seq Check: 4314 from: 1 to: 3632
pF1KB6965 3632 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 616 670 616
Size: 54 3578
Acc65I G'GTAC_C
Cuts at: 648 648
Size: 3632
AclI AA'CG_TT
Cuts at: 3472 3472
Size: 3632
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1457 1889 2636 2904 1457
Size: 432 747 268 2185
Fragments arranged by size:
2185 747 432 268
AflIII A'CryG_T
Cuts at: 578 984 2088 3065 578
Size: 406 1104 977 1145
Fragments arranged by size:
1145 1104 977 406
AgeI A'CCGG_T
Cuts at: 2020 2020
Size: 3632
AlwNI CAG_nnn'CTG
Cuts at: 231 937 2504 231
Size: 706 1567 1359
Fragments arranged by size:
1567 1359 706
ApaLI G'TGCA_C
Cuts at: 2402 2402
Size: 3632
ApoI r'AATT_y
Cuts at: 636 2985 3241 636
Size: 2349 256 1027
Fragments arranged by size:
2349 1027 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3632
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
AvaI C'yCGr_G
Cuts at: 299 652 299
Size: 353 3279
BamHI G'GATC_C
Cuts at: 657 657
Size: 3632
BanI G'GyrC_C
Cuts at: 608 648 1062 1310 1345 608
Size: 40 414 248 35 2895
Fragments arranged by size:
2895 414 248 40 35
BanII G_rGCy'C
Cuts at: 646 1676 646
Size: 1030 2602
BbeI G_GCGC'C
Cuts at: 612 1066 1314 612
Size: 454 248 2930
Fragments arranged by size:
2930 454 248
BciVI GTATCCnnnnn_n'
Cuts at: 1530 2291 3632 1530
Size: 761 1341 1530
Fragments arranged by size:
1530 1341 761
BclI T'GATC_A
Cuts at: 1152 1152
Size: 3632
BglI GCCn_nnn'nGGC
Cuts at: 301 301
Size: 3632
BglII A'GATC_T
Cuts at: 1147 3184 1147
Size: 2037 1595
BlpI GC'TnA_GC
Cuts at: 744 744
Size: 3632
Bme1580I G_kGCm'C
Cuts at: 1257 1350 2406 1257
Size: 93 1056 2483
Fragments arranged by size:
2483 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 844 925 1260 3404 844
Size: 81 335 2144 1072
Fragments arranged by size:
2144 1072 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1999 1999
Size: 3632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 488
Size: 3632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1687 2179 2477 808
Size: 879 492 298 1963
Fragments arranged by size:
1963 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3336 3336
Size: 3632
BsaAI yAC'GTr
Cuts at: 985 985
Size: 3632
BsaBI GATnn'nnATC
Cuts at: 6 1146 3183 6
Size: 1140 2037 455
Fragments arranged by size:
2037 1140 455
BsaHI Gr'CG_yC
Cuts at: 609 1063 1311 2013 3509 609
Size: 454 248 702 1496 732
Fragments arranged by size:
1496 732 702 454 248
BsaWI w'CCGG_w
Cuts at: 821 1342 2020 2294 2441 2852 821
Size: 521 678 274 147 411 1601
Fragments arranged by size:
1601 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 461 491 461
Size: 30 3602
BseYI C'CCAG_C
Cuts at: 536 1057 1815 2392 2888 536
Size: 521 758 577 496 1280
Fragments arranged by size:
1280 758 577 521 496
BsiEI CG_ry'CG
Cuts at: 104 1174 1220 2428 104
Size: 1070 46 1208 1308
Fragments arranged by size:
1308 1208 1070 46
BsiHKAI G_wGCw'C
Cuts at: 551 646 1424 1614 2406 551
Size: 95 778 190 792 1777
Fragments arranged by size:
1777 792 778 190 95
BsmI GAATG_Cn'
Cuts at: 297 607 297
Size: 310 3322
Bsp1286I G_dGCh'C
Cuts at: 551 646 1257 1350 1424 1614 1676 2406 551
Size: 95 611 93 74 190 62 730 1777
Fragments arranged by size:
1777 730 611 190 95 93 74 62
BspEI T'CCGG_A
Cuts at: 2852 2852
Size: 3632
BspHI T'CATG_A
Cuts at: 308 551 2808 308
Size: 243 2257 1132
Fragments arranged by size:
2257 1132 243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 272 682 896 1198 1579 272
Size: 410 214 302 381 2325
Fragments arranged by size:
2325 410 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1924 1978 3462 3627 50
Size: 1874 54 1484 165 55
Fragments arranged by size:
1874 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1544 1544
Size: 3632
BsrFI r'CCGG_y
Cuts at: 200 605 1630 1811 2020 200
Size: 405 1025 181 209 1812
Fragments arranged by size:
1812 1025 405 209 181
BsrGI T'GTAC_A
Cuts at: 110 110
Size: 3632
BssSI C'ACGA_G
Cuts at: 1903 2261 1903
Size: 358 3274
BstAPI GCAn_nnn'nTGC
Cuts at: 257 1009 257
Size: 752 2880
BstBI TT'CG_AA
Cuts at: 1993 1993
Size: 3632
BstXI CCAn_nnnn'nTGG
Cuts at: 162 162
Size: 3632
BstYI r'GATC_y
Cuts at: 1 657 1124 1147 1482 1728 2740 2826 2838
Size: 656 467 23 335 246 1012 86 12
Cuts at: 2838 3184 1
Size: 346 449
Fragments arranged by size:
1012 656 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 173 173
Size: 3632
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 264 1582 1763 1926 264
Size: 1318 181 163 1970
Fragments arranged by size:
1970 1318 181 163
BtsI GCAGTG_nn'
Cuts at: 551 875 551
Size: 324 3308
DraI TTT'AAA
Cuts at: 631 631
Size: 3632
DrdI GACnn_nn'nnGTC
Cuts at: 1338 2196 1338
Size: 858 2774
EaeI y'GGCC_r
Cuts at: 170 455 1217 1391 1782 1809 3154 170
Size: 285 762 174 391 27 1345 648
Fragments arranged by size:
1345 762 648 391 285 174 27
EagI C'GGCC_G
Cuts at: 1217 1217
Size: 3632
EarI CTCTTCn'nnn_
Cuts at: 1655 1865 1655
Size: 210 3422
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1840 2150 2296 3442 1840
Size: 310 146 1146 2030
Fragments arranged by size:
2030 1146 310 146
EcoICRI GAG'CTC
Cuts at: 644 644
Size: 3632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 1457 1889 2636 2904 488
Size: 969 432 747 268 1216
Fragments arranged by size:
1216 969 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 212 228 771 212
Size: 16 543 3073
Fragments arranged by size:
3073 543 16
EcoRI G'AATT_C
Cuts at: 636 636
Size: 3632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1651 1651
Size: 3632
FspI TGC'GCA
Cuts at: 1413 1413
Size: 3632
HaeII r_GCGC'y
Cuts at: 513 612 1066 1314 2336 3315 513
Size: 99 454 248 1022 979 830
Fragments arranged by size:
1022 979 830 454 248 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 461 493 1474 1504 1506 1536 461
Size: 32 981 30 2 30 2557
Fragments arranged by size:
2557 981 32 30 30 2
HincII GTy'rAC
Cuts at: 671 671
Size: 3632
KasI G'GCGC_C
Cuts at: 608 1062 1310 608
Size: 454 248 2930
Fragments arranged by size:
2930 454 248
KpnI G_GTAC'C
Cuts at: 652 652
Size: 3632
MluI A'CGCG_T
Cuts at: 3065 3065
Size: 3632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2303 2487 2303
Size: 184 3448
MscI TGG'CCA
Cuts at: 172 457 1393 172
Size: 285 936 2411
Fragments arranged by size:
2411 936 285
MslI CAynn'nnrTG
Cuts at: 346 1621 1748 346
Size: 1275 127 2230
Fragments arranged by size:
2230 1275 127
MspA1I CmG'CkG
Cuts at: 743 1057 1417 2430 2675 743
Size: 314 360 1013 245 1700
Fragments arranged by size:
1700 1013 360 314 245
NaeI GCC'GGC
Cuts at: 607 1813 607
Size: 1206 2426
NarI GG'CG_CC
Cuts at: 609 1063 1311 609
Size: 454 248 2930
Fragments arranged by size:
2930 454 248
NcoI C'CATG_G
Cuts at: 173 173
Size: 3632
NgoMIV G'CCGG_C
Cuts at: 605 1811 605
Size: 1206 2426
NspI r_CATG'y
Cuts at: 582 685 1185 2092 582
Size: 103 500 907 2122
Fragments arranged by size:
2122 907 500 103
PciI A'CATG_T
Cuts at: 578 2088 578
Size: 1510 2122
PmeI GTTT'AAAC
Cuts at: 631 631
Size: 3632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2771 2803 2771
Size: 32 3600
PpuMI rG'GwC_Cy
Cuts at: 212 212
Size: 3632
PstI C_TGCA'G
Cuts at: 269 679 1364 269
Size: 410 685 2537
Fragments arranged by size:
2537 685 410
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3632
PvuII CAG'CTG
Cuts at: 1057 1417 1057
Size: 360 3272
RsrII CG'GwC_CG
Cuts at: 1827 1827
Size: 3632
SacI G_AGCT'C
Cuts at: 646 646
Size: 3632
SalI G'TCGA_C
Cuts at: 669 669
Size: 3632
SapI GCTCTTCn'nnn_
Cuts at: 1655 1865 1655
Size: 210 3422
SbfI CC_TGCA'GG
Cuts at: 679 679
Size: 3632
ScaI AGT'ACT
Cuts at: 3149 3149
Size: 3632
SfcI C'TryA_G
Cuts at: 33 265 675 1360 2353 2544 33
Size: 232 410 685 993 191 1121
Fragments arranged by size:
1121 993 685 410 232 191
SfoI GGC'GCC
Cuts at: 610 1064 1312 610
Size: 454 248 2930
Fragments arranged by size:
2930 454 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
SmaI CCC'GGG
Cuts at: 301 654 301
Size: 353 3279
SmlI C'TyrA_G
Cuts at: 787 1702 2194 2456 787
Size: 915 492 262 1963
Fragments arranged by size:
1963 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3632
SphI G_CATG'C
Cuts at: 685 1185 685
Size: 500 3132
StyI C'CwwG_G
Cuts at: 173 452 766 1120 173
Size: 279 314 354 2685
Fragments arranged by size:
2685 354 314 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 112 135 528 1215 1348 1807 2016 112
Size: 23 393 687 133 459 209 1728
Fragments arranged by size:
1728 687 459 393 209 133 23
TatI w'GTAC_w
Cuts at: 110 3147 110
Size: 3037 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3600
Tth111I GACn'n_nGTC
Cuts at: 1429 1429
Size: 3632
XbaI T'CTAG_A
Cuts at: 663 663
Size: 3632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 162 1127 162
Size: 965 2667
XmaI C'CCGG_G
Cuts at: 299 652 299
Size: 353 3279
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI