Restriction map for
pF1KB6969
(Circular) (Six-base) MAPSORT of: pF1KB6969.seq Check: 1292 from: 1 to: 3761
pF1KB6969 3761 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 799 799
Size: 3761
Acc65I G'GTAC_C
Cuts at: 777 777
Size: 3761
AclI AA'CG_TT
Cuts at: 755 3601 755
Size: 2846 915
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1586 2018 2765 3033 1586
Size: 432 747 268 2314
Fragments arranged by size:
2314 747 432 268
AflIII A'CryG_T
Cuts at: 1113 2217 3194 1113
Size: 1104 977 1680
Fragments arranged by size:
1680 1104 977
AgeI A'CCGG_T
Cuts at: 2149 2149
Size: 3761
AlwNI CAG_nnn'CTG
Cuts at: 151 1066 2633 151
Size: 915 1567 1279
Fragments arranged by size:
1567 1279 915
ApaI G_GGCC'C
Cuts at: 390 390
Size: 3761
ApaLI G'TGCA_C
Cuts at: 2531 2531
Size: 3761
ApoI r'AATT_y
Cuts at: 541 765 3114 3370 541
Size: 224 2349 256 932
Fragments arranged by size:
2349 932 256 224
AseI AT'TA_AT
Cuts at: 20 20
Size: 3761
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
AvaI C'yCGr_G
Cuts at: 781 781
Size: 3761
BamHI G'GATC_C
Cuts at: 786 786
Size: 3761
BanI G'GyrC_C
Cuts at: 777 1191 1439 1474 777
Size: 414 248 35 3064
Fragments arranged by size:
3064 414 248 35
BanII G_rGCy'C
Cuts at: 390 668 775 1805 390
Size: 278 107 1030 2346
Fragments arranged by size:
2346 1030 278 107
BbeI G_GCGC'C
Cuts at: 1195 1443 1195
Size: 248 3513
BciVI GTATCCnnnnn_n'
Cuts at: 1659 2420 3761 1659
Size: 761 1341 1659
Fragments arranged by size:
1659 1341 761
BclI T'GATC_A
Cuts at: 1281 1281
Size: 3761
BglII A'GATC_T
Cuts at: 161 326 1276 3313 161
Size: 165 950 2037 609
Fragments arranged by size:
2037 950 609 165
BlpI GC'TnA_GC
Cuts at: 873 873
Size: 3761
Bme1580I G_kGCm'C
Cuts at: 390 1386 1479 2535 390
Size: 996 93 1056 1616
Fragments arranged by size:
1616 1056 996 93
BmrI ACTGGGnnnn_n'
Cuts at: 459 973 1054 1389 3533 459
Size: 514 81 335 2144 687
Fragments arranged by size:
2144 687 514 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 3761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 697 671
Size: 26 3735
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1816 2308 2606 937
Size: 879 492 298 2092
Fragments arranged by size:
2092 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3465 3465
Size: 3761
BsaAI yAC'GTr
Cuts at: 1114 1114
Size: 3761
BsaBI GATnn'nnATC
Cuts at: 6 1275 3312 6
Size: 1269 2037 455
Fragments arranged by size:
2037 1269 455
BsaHI Gr'CG_yC
Cuts at: 1192 1440 2142 3638 1192
Size: 248 702 1496 1315
Fragments arranged by size:
1496 1315 702 248
BsaWI w'CCGG_w
Cuts at: 950 1471 2149 2423 2570 2981 950
Size: 521 678 274 147 411 1730
Fragments arranged by size:
1730 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 669 699 669
Size: 30 3731
BseYI C'CCAG_C
Cuts at: 151 186 726 1186 1944 2521 3017 151
Size: 35 540 460 758 577 496 895
Fragments arranged by size:
895 758 577 540 496 460 35
BsiEI CG_ry'CG
Cuts at: 104 1303 1349 2557 104
Size: 1199 46 1208 1308
Fragments arranged by size:
1308 1208 1199 46
BsiHKAI G_wGCw'C
Cuts at: 265 687 775 1553 1743 2535 265
Size: 422 88 778 190 792 1491
Fragments arranged by size:
1491 792 778 422 190 88
BsmI GAATG_Cn'
Cuts at: 678 678
Size: 3761
Bsp1286I G_dGCh'C
Cuts at: 265 390 668 687 775 1386 1479 1553 1743
Size: 125 278 19 88 611 93 74 190
Cuts at: 1743 1805 2535 265
Size: 62 730 1491
Fragments arranged by size:
1491 730 611 278 190 125 93 88
74 62 19
BspEI T'CCGG_A
Cuts at: 2981 2981
Size: 3761
BspHI T'CATG_A
Cuts at: 2937 2937
Size: 3761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 811 1025 1327 1708 811
Size: 214 302 381 2864
Fragments arranged by size:
2864 381 302 214
BsrBI CCG'CTC
Cuts at: 50 274 351 2053 2107 3591 3756 50
Size: 224 77 1702 54 1484 165 55
Fragments arranged by size:
1702 1484 224 165 77 55 54
BsrDI GCAATG_nn'
Cuts at: 491 617 1673 491
Size: 126 1056 2579
Fragments arranged by size:
2579 1056 126
BsrFI r'CCGG_y
Cuts at: 1759 1940 2149 1759
Size: 181 209 3371
Fragments arranged by size:
3371 209 181
BssSI C'ACGA_G
Cuts at: 247 2032 2390 247
Size: 1785 358 1618
Fragments arranged by size:
1785 1618 358
BstAPI GCAn_nnn'nTGC
Cuts at: 607 682 1138 607
Size: 75 456 3230
Fragments arranged by size:
3230 456 75
BstBI TT'CG_AA
Cuts at: 2122 2122
Size: 3761
BstYI r'GATC_y
Cuts at: 1 161 326 786 1253 1276 1611 1857 2869
Size: 160 165 460 467 23 335 246 1012
Cuts at: 2869 2955 2967 3313 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 460 449 346 335 246 165
160 86 23 12
BtgI C'CryG_G
Cuts at: 107 536 107
Size: 429 3332
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1711 1892 2055 1711
Size: 181 163 3417
Fragments arranged by size:
3417 181 163
BtsI GCAGTG_nn'
Cuts at: 1004 1004
Size: 3761
DraI TTT'AAA
Cuts at: 760 760
Size: 3761
DrdI GACnn_nn'nnGTC
Cuts at: 313 1467 2325 313
Size: 1154 858 1749
Fragments arranged by size:
1749 1154 858
EaeI y'GGCC_r
Cuts at: 521 1346 1520 1911 1938 3283 521
Size: 825 174 391 27 1345 999
Fragments arranged by size:
1345 999 825 391 174 27
EagI C'GGCC_G
Cuts at: 1346 1346
Size: 3761
EarI CTCTTCn'nnn_
Cuts at: 1784 1994 1784
Size: 210 3551
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1969 2279 2425 3571 1969
Size: 310 146 1146 2159
Fragments arranged by size:
2159 1146 310 146
EcoICRI GAG'CTC
Cuts at: 773 773
Size: 3761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 697 1586 2018 2765 3033 671
Size: 26 889 432 747 268 1399
Fragments arranged by size:
1399 889 747 432 268 26
EcoO109I rG'GnC_Cy
Cuts at: 386 900 386
Size: 514 3247
EcoRI G'AATT_C
Cuts at: 541 765 541
Size: 224 3537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1780 1780
Size: 3761
FspI TGC'GCA
Cuts at: 1542 1542
Size: 3761
HaeII r_GCGC'y
Cuts at: 1195 1443 2465 3444 1195
Size: 248 1022 979 1512
Fragments arranged by size:
1512 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1603 1633 1635 1665 1603
Size: 30 2 30 3699
Fragments arranged by size:
3699 30 30 2
HincII GTy'rAC
Cuts at: 800 800
Size: 3761
HindIII A'AGCT_T
Cuts at: 649 649
Size: 3761
KasI G'GCGC_C
Cuts at: 1191 1439 1191
Size: 248 3513
KpnI G_GTAC'C
Cuts at: 781 781
Size: 3761
MfeI C'AATT_G
Cuts at: 266 266
Size: 3761
MluI A'CGCG_T
Cuts at: 3194 3194
Size: 3761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2432 2616 2432
Size: 184 3577
MscI TGG'CCA
Cuts at: 523 1522 523
Size: 999 2762
MslI CAynn'nnrTG
Cuts at: 1750 1877 1750
Size: 127 3634
MspA1I CmG'CkG
Cuts at: 148 340 872 1186 1546 2559 2804 148
Size: 192 532 314 360 1013 245 1105
Fragments arranged by size:
1105 1013 532 360 314 245 192
NaeI GCC'GGC
Cuts at: 1942 1942
Size: 3761
NarI GG'CG_CC
Cuts at: 1192 1440 1192
Size: 248 3513
NcoI C'CATG_G
Cuts at: 107 107
Size: 3761
NgoMIV G'CCGG_C
Cuts at: 1940 1940
Size: 3761
NspI r_CATG'y
Cuts at: 814 1314 2221 814
Size: 500 907 2354
Fragments arranged by size:
2354 907 500
PciI A'CATG_T
Cuts at: 2217 2217
Size: 3761
PflMI CCAn_nnn'nTGG
Cuts at: 151 449 151
Size: 298 3463
PfoI T'CCnGG_A
Cuts at: 545 713 545
Size: 168 3593
PmeI GTTT'AAAC
Cuts at: 760 760
Size: 3761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2932 2900
Size: 32 3729
PspOMI G'GGCC_C
Cuts at: 386 386
Size: 3761
PstI C_TGCA'G
Cuts at: 751 808 1493 751
Size: 57 685 3019
Fragments arranged by size:
3019 685 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3761
PvuII CAG'CTG
Cuts at: 148 340 1186 1546 148
Size: 192 846 360 2363
Fragments arranged by size:
2363 846 360 192
RsrII CG'GwC_CG
Cuts at: 1956 1956
Size: 3761
SacI G_AGCT'C
Cuts at: 775 775
Size: 3761
SalI G'TCGA_C
Cuts at: 798 798
Size: 3761
SapI GCTCTTCn'nnn_
Cuts at: 1784 1994 1784
Size: 210 3551
SbfI CC_TGCA'GG
Cuts at: 808 808
Size: 3761
ScaI AGT'ACT
Cuts at: 3278 3278
Size: 3761
SfcI C'TryA_G
Cuts at: 33 463 747 804 1489 2482 2673 33
Size: 430 284 57 685 993 191 1121
Fragments arranged by size:
1121 993 685 430 284 191 57
SfoI GGC'GCC
Cuts at: 1193 1441 1193
Size: 248 3513
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
SmaI CCC'GGG
Cuts at: 783 783
Size: 3761
SmlI C'TyrA_G
Cuts at: 916 1831 2323 2585 916
Size: 915 492 262 2092
Fragments arranged by size:
2092 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3761
SphI G_CATG'C
Cuts at: 814 1314 814
Size: 500 3261
StyI C'CwwG_G
Cuts at: 107 895 1249 107
Size: 788 354 2619
Fragments arranged by size:
2619 788 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 441 1344 1477 1936 2145 441
Size: 903 133 459 209 2057
Fragments arranged by size:
2057 903 459 209 133
TatI w'GTAC_w
Cuts at: 374 3276 374
Size: 2902 859
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 3729
Tth111I GACn'n_nGTC
Cuts at: 1558 1558
Size: 3761
XbaI T'CTAG_A
Cuts at: 792 792
Size: 3761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 676 1256 676
Size: 580 3181
XmaI C'CCGG_G
Cuts at: 781 781
Size: 3761
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI