Restriction map for
pF1KB6970
(Circular) (Six-base) MAPSORT of: pF1KB6970.seq Check: 4407 from: 1 to: 3836
pF1KB6970 3836 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 874 874
Size: 3836
Acc65I G'GTAC_C
Cuts at: 852 852
Size: 3836
AclI AA'CG_TT
Cuts at: 3676 3676
Size: 3836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 1661 2093 2840 3108 611
Size: 1050 432 747 268 1339
Fragments arranged by size:
1339 1050 747 432 268
AflIII A'CryG_T
Cuts at: 788 1188 2292 3269 788
Size: 400 1104 977 1355
Fragments arranged by size:
1355 1104 977 400
AgeI A'CCGG_T
Cuts at: 2224 2224
Size: 3836
AlwNI CAG_nnn'CTG
Cuts at: 1141 2708 1141
Size: 1567 2269
ApaLI G'TGCA_C
Cuts at: 2606 2606
Size: 3836
ApoI r'AATT_y
Cuts at: 780 840 3189 3445 780
Size: 60 2349 256 1171
Fragments arranged by size:
2349 1171 256 60
AseI AT'TA_AT
Cuts at: 20 20
Size: 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
AvaI C'yCGr_G
Cuts at: 608 856 608
Size: 248 3588
BamHI G'GATC_C
Cuts at: 224 861 224
Size: 637 3199
BanI G'GyrC_C
Cuts at: 499 634 852 1266 1514 1549 499
Size: 135 218 414 248 35 2786
Fragments arranged by size:
2786 414 248 218 135 35
BanII G_rGCy'C
Cuts at: 290 646 850 1880 290
Size: 356 204 1030 2246
Fragments arranged by size:
2246 1030 356 204
BbeI G_GCGC'C
Cuts at: 1270 1518 1270
Size: 248 3588
BbsI GAAGACnn'nnnn_
Cuts at: 689 689
Size: 3836
BciVI GTATCCnnnnn_n'
Cuts at: 1734 2495 3836 1734
Size: 761 1341 1734
Fragments arranged by size:
1734 1341 761
BclI T'GATC_A
Cuts at: 1356 1356
Size: 3836
BglI GCCn_nnn'nGGC
Cuts at: 193 193
Size: 3836
BglII A'GATC_T
Cuts at: 1351 3388 1351
Size: 2037 1799
BlpI GC'TnA_GC
Cuts at: 948 948
Size: 3836
Bme1580I G_kGCm'C
Cuts at: 639 1461 1554 2610 639
Size: 822 93 1056 1865
Fragments arranged by size:
1865 1056 822 93
BmrI ACTGGGnnnn_n'
Cuts at: 1048 1129 1464 3608 1048
Size: 81 335 2144 1276
Fragments arranged by size:
2144 1276 335 81
BmtI G_CTAG'C
Cuts at: 760 760
Size: 3836
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 3836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1891 2383 2681 1012
Size: 879 492 298 2167
Fragments arranged by size:
2167 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3540 3540
Size: 3836
BsaAI yAC'GTr
Cuts at: 1189 1189
Size: 3836
BsaBI GATnn'nnATC
Cuts at: 6 491 1350 3387 6
Size: 485 859 2037 455
Fragments arranged by size:
2037 859 485 455
BsaHI Gr'CG_yC
Cuts at: 1267 1515 2217 3713 1267
Size: 248 702 1496 1390
Fragments arranged by size:
1496 1390 702 248
BsaWI w'CCGG_w
Cuts at: 1025 1546 2224 2498 2645 3056 1025
Size: 521 678 274 147 411 1805
Fragments arranged by size:
1805 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 138 168 138
Size: 30 3806
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 554 742 554
Size: 188 3648
BseYI C'CCAG_C
Cuts at: 289 560 1261 2019 2596 3092 289
Size: 271 701 758 577 496 1033
Fragments arranged by size:
1033 758 701 577 496 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 787
Size: 3836
BsiEI CG_ry'CG
Cuts at: 104 1378 1424 2632 104
Size: 1274 46 1208 1308
Fragments arranged by size:
1308 1274 1208 46
BsiHKAI G_wGCw'C
Cuts at: 646 850 1628 1818 2610 646
Size: 204 778 190 792 1872
Fragments arranged by size:
1872 792 778 204 190
BsmI GAATG_Cn'
Cuts at: 744 744
Size: 3836
Bsp1286I G_dGCh'C
Cuts at: 290 639 646 850 1461 1554 1628 1818 1880
Size: 349 7 204 611 93 74 190 62
Cuts at: 1880 2610 290
Size: 730 1516
Fragments arranged by size:
1516 730 611 349 204 190 93 74
62 7
BspEI T'CCGG_A
Cuts at: 3056 3056
Size: 3836
BspHI T'CATG_A
Cuts at: 3012 3012
Size: 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 886 1100 1402 1783 886
Size: 214 302 381 2939
Fragments arranged by size:
2939 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2128 2182 3666 3831 50
Size: 2078 54 1484 165 55
Fragments arranged by size:
2078 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1748 1748
Size: 3836
BsrFI r'CCGG_y
Cuts at: 1834 2015 2224 1834
Size: 181 209 3446
Fragments arranged by size:
3446 209 181
BsrGI T'GTAC_A
Cuts at: 594 594
Size: 3836
BssSI C'ACGA_G
Cuts at: 2107 2465 2107
Size: 358 3478
BstAPI GCAn_nnn'nTGC
Cuts at: 1213 1213
Size: 3836
BstBI TT'CG_AA
Cuts at: 2197 2197
Size: 3836
BstYI r'GATC_y
Cuts at: 1 224 492 861 1328 1351 1686 1932 2944
Size: 223 268 369 467 23 335 246 1012
Cuts at: 2944 3030 3042 3388 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 369 346 335 268 246
223 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 1967 2130 1786
Size: 181 163 3492
Fragments arranged by size:
3492 181 163
BtsI GCAGTG_nn'
Cuts at: 1079 1079
Size: 3836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 413 448 413
Size: 35 3801
DraI TTT'AAA
Cuts at: 835 835
Size: 3836
DrdI GACnn_nn'nnGTC
Cuts at: 1542 2400 1542
Size: 858 2978
EaeI y'GGCC_r
Cuts at: 774 1421 1595 1986 2013 3358 774
Size: 647 174 391 27 1345 1252
Fragments arranged by size:
1345 1252 647 391 174 27
EagI C'GGCC_G
Cuts at: 1421 1421
Size: 3836
EarI CTCTTCn'nnn_
Cuts at: 256 806 1859 2069 256
Size: 550 1053 210 2023
Fragments arranged by size:
2023 1053 550 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2044 2354 2500 3646 2044
Size: 310 146 1146 2234
Fragments arranged by size:
2234 1146 310 146
EcoICRI GAG'CTC
Cuts at: 644 848 644
Size: 204 3632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 1661 2093 2840 3108 611
Size: 1050 432 747 268 1339
Fragments arranged by size:
1339 1050 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 556 587 975 556
Size: 31 388 3417
Fragments arranged by size:
3417 388 31
EcoRI G'AATT_C
Cuts at: 840 840
Size: 3836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3836
FspI TGC'GCA
Cuts at: 1617 1617
Size: 3836
HaeII r_GCGC'y
Cuts at: 1270 1518 2540 3519 1270
Size: 248 1022 979 1587
Fragments arranged by size:
1587 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 306 338 1678 1708 1710 1740 306
Size: 32 1340 30 2 30 2402
Fragments arranged by size:
2402 1340 32 30 30 2
HincII GTy'rAC
Cuts at: 875 875
Size: 3836
HindIII A'AGCT_T
Cuts at: 202 202
Size: 3836
KasI G'GCGC_C
Cuts at: 1266 1514 1266
Size: 248 3588
KpnI G_GTAC'C
Cuts at: 856 856
Size: 3836
MfeI C'AATT_G
Cuts at: 614 614
Size: 3836
MluI A'CGCG_T
Cuts at: 3269 3269
Size: 3836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2507 2691 2507
Size: 184 3652
MscI TGG'CCA
Cuts at: 776 1597 776
Size: 821 3015
MslI CAynn'nnrTG
Cuts at: 1825 1952 1825
Size: 127 3709
MspA1I CmG'CkG
Cuts at: 564 947 1261 1621 2634 2879 564
Size: 383 314 360 1013 245 1521
Fragments arranged by size:
1521 1013 383 360 314 245
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 3836
NarI GG'CG_CC
Cuts at: 1267 1515 1267
Size: 248 3588
NdeI CA'TA_TG
Cuts at: 572 572
Size: 3836
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 3836
NheI G'CTAG_C
Cuts at: 756 756
Size: 3836
NspI r_CATG'y
Cuts at: 792 889 1389 2296 792
Size: 97 500 907 2332
Fragments arranged by size:
2332 907 500 97
PciI A'CATG_T
Cuts at: 788 2292 788
Size: 1504 2332
PmeI GTTT'AAAC
Cuts at: 835 835
Size: 3836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3804
PpuMI rG'GwC_Cy
Cuts at: 556 587 556
Size: 31 3805
PspXI vC'TCGA_Gb
Cuts at: 608 608
Size: 3836
PstI C_TGCA'G
Cuts at: 883 1568 883
Size: 685 3151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3836
PvuII CAG'CTG
Cuts at: 564 1261 1621 564
Size: 697 360 2779
Fragments arranged by size:
2779 697 360
RsrII CG'GwC_CG
Cuts at: 2031 2031
Size: 3836
SacI G_AGCT'C
Cuts at: 646 850 646
Size: 204 3632
SalI G'TCGA_C
Cuts at: 873 873
Size: 3836
SapI GCTCTTCn'nnn_
Cuts at: 1859 2069 1859
Size: 210 3626
SbfI CC_TGCA'GG
Cuts at: 883 883
Size: 3836
ScaI AGT'ACT
Cuts at: 3353 3353
Size: 3836
SfcI C'TryA_G
Cuts at: 33 212 879 1564 2557 2748 33
Size: 179 667 685 993 191 1121
Fragments arranged by size:
1121 993 685 667 191 179
SfoI GGC'GCC
Cuts at: 1268 1516 1268
Size: 248 3588
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
SmaI CCC'GGG
Cuts at: 858 858
Size: 3836
SmlI C'TyrA_G
Cuts at: 608 991 1906 2398 2660 608
Size: 383 915 492 262 1784
Fragments arranged by size:
1784 915 492 383 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3836
SphI G_CATG'C
Cuts at: 889 1389 889
Size: 500 3336
StyI C'CwwG_G
Cuts at: 970 1324 970
Size: 354 3482
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1419 1552 2011 2220 1419
Size: 133 459 209 3035
Fragments arranged by size:
3035 459 209 133
TatI w'GTAC_w
Cuts at: 594 3351 594
Size: 2757 1079
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 692 724 3353 3385 692
Size: 32 2629 32 1143
Fragments arranged by size:
2629 1143 32 32
Tth111I GACn'n_nGTC
Cuts at: 1633 1633
Size: 3836
XbaI T'CTAG_A
Cuts at: 867 867
Size: 3836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1331 1331
Size: 3836
XhoI C'TCGA_G
Cuts at: 608 608
Size: 3836
XmaI C'CCGG_G
Cuts at: 856 856
Size: 3836
XmnI GAAnn'nnTTC
Cuts at: 270 270
Size: 3836
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NheI NspI PciI PmeI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BpmI Bpu10I BsiWI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI FspAI
HpaI NcoI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
ZraI