Restriction map for
pF1KB6971
(Circular) (Six-base) MAPSORT of: pF1KB6971.seq Check: 4197 from: 1 to: 3761
pF1KB6971 3761 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 799 799
Size: 3761
Acc65I G'GTAC_C
Cuts at: 777 777
Size: 3761
AclI AA'CG_TT
Cuts at: 3601 3601
Size: 3761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 709 1586 2018 2765 3033 517
Size: 192 877 432 747 268 1245
Fragments arranged by size:
1245 877 747 432 268 192
AfeI AGC'GCT
Cuts at: 472 472
Size: 3761
AflIII A'CryG_T
Cuts at: 1113 2217 3194 1113
Size: 1104 977 1680
Fragments arranged by size:
1680 1104 977
AgeI A'CCGG_T
Cuts at: 2149 2149
Size: 3761
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 296 328 296
Size: 32 3729
AlwNI CAG_nnn'CTG
Cuts at: 1066 2633 1066
Size: 1567 2194
ApaI G_GGCC'C
Cuts at: 227 227
Size: 3761
ApaLI G'TGCA_C
Cuts at: 2531 2531
Size: 3761
ApoI r'AATT_y
Cuts at: 382 765 3114 3370 382
Size: 383 2349 256 773
Fragments arranged by size:
2349 773 383 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3761
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
AvaI C'yCGr_G
Cuts at: 781 781
Size: 3761
BamHI G'GATC_C
Cuts at: 786 786
Size: 3761
BanI G'GyrC_C
Cuts at: 777 1191 1439 1474 777
Size: 414 248 35 3064
Fragments arranged by size:
3064 414 248 35
BanII G_rGCy'C
Cuts at: 227 775 1805 227
Size: 548 1030 2183
Fragments arranged by size:
2183 1030 548
BbeI G_GCGC'C
Cuts at: 1195 1443 1195
Size: 248 3513
BbsI GAAGACnn'nnnn_
Cuts at: 165 165
Size: 3761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3727
BciVI GTATCCnnnnn_n'
Cuts at: 1659 2420 3761 1659
Size: 761 1341 1659
Fragments arranged by size:
1659 1341 761
BclI T'GATC_A
Cuts at: 698 1281 698
Size: 583 3178
BglII A'GATC_T
Cuts at: 1276 3313 1276
Size: 2037 1724
BlpI GC'TnA_GC
Cuts at: 873 873
Size: 3761
Bme1580I G_kGCm'C
Cuts at: 227 1386 1479 2535 227
Size: 1159 93 1056 1453
Fragments arranged by size:
1453 1159 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 552 973 1054 1389 3533 552
Size: 421 81 335 2144 780
Fragments arranged by size:
2144 780 421 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 3761
Bpu10I CC'TnA_GC
Cuts at: 342 342
Size: 3761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1816 2308 2606 937
Size: 879 492 298 2092
Fragments arranged by size:
2092 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 301 3465 301
Size: 3164 597
BsaAI yAC'GTr
Cuts at: 1114 1114
Size: 3761
BsaBI GATnn'nnATC
Cuts at: 6 1275 3312 6
Size: 1269 2037 455
Fragments arranged by size:
2037 1269 455
BsaHI Gr'CG_yC
Cuts at: 1192 1440 2142 3638 1192
Size: 248 702 1496 1315
Fragments arranged by size:
1496 1315 702 248
BsaWI w'CCGG_w
Cuts at: 950 1471 2149 2423 2570 2981 950
Size: 521 678 274 147 411 1730
Fragments arranged by size:
1730 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 515 1186 1944 2521 3017 515
Size: 671 758 577 496 1259
Fragments arranged by size:
1259 758 671 577 496
BsiEI CG_ry'CG
Cuts at: 104 206 1303 1349 2557 104
Size: 102 1097 46 1208 1308
Fragments arranged by size:
1308 1208 1097 102 46
BsiHKAI G_wGCw'C
Cuts at: 143 661 775 1553 1743 2535 143
Size: 518 114 778 190 792 1369
Fragments arranged by size:
1369 792 778 518 190 114
Bsp1286I G_dGCh'C
Cuts at: 143 227 661 775 1386 1479 1553 1743 1805
Size: 84 434 114 611 93 74 190 62
Cuts at: 1805 2535 143
Size: 730 1369
Fragments arranged by size:
1369 730 611 434 190 114 93 84
74 62
BspEI T'CCGG_A
Cuts at: 2981 2981
Size: 3761
BspHI T'CATG_A
Cuts at: 407 2937 407
Size: 2530 1231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 811 1025 1327 1708 811
Size: 214 302 381 2864
Fragments arranged by size:
2864 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2053 2107 3591 3756 50
Size: 2003 54 1484 165 55
Fragments arranged by size:
2003 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1673 1673
Size: 3761
BsrFI r'CCGG_y
Cuts at: 1759 1940 2149 1759
Size: 181 209 3371
Fragments arranged by size:
3371 209 181
BssSI C'ACGA_G
Cuts at: 2032 2390 2032
Size: 358 3403
BstAPI GCAn_nnn'nTGC
Cuts at: 1138 1138
Size: 3761
BstBI TT'CG_AA
Cuts at: 2122 2122
Size: 3761
BstYI r'GATC_y
Cuts at: 1 786 1253 1276 1611 1857 2869 2955 2967
Size: 785 467 23 335 246 1012 86 12
Cuts at: 2967 3313 1
Size: 346 449
Fragments arranged by size:
1012 785 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 144 687 729 1711 1892 2055 144
Size: 543 42 982 181 163 1850
Fragments arranged by size:
1850 982 543 181 163 42
BtsI GCAGTG_nn'
Cuts at: 1004 1004
Size: 3761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 305 340 305
Size: 35 3726
DraI TTT'AAA
Cuts at: 760 760
Size: 3761
DrdI GACnn_nn'nnGTC
Cuts at: 1467 2325 1467
Size: 858 2903
EaeI y'GGCC_r
Cuts at: 203 527 1346 1520 1911 1938 3283 203
Size: 324 819 174 391 27 1345 681
Fragments arranged by size:
1345 819 681 391 324 174 27
EagI C'GGCC_G
Cuts at: 203 1346 203
Size: 1143 2618
EarI CTCTTCn'nnn_
Cuts at: 304 1784 1994 304
Size: 1480 210 2071
Fragments arranged by size:
2071 1480 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 434 1969 2279 2425 3571 434
Size: 1535 310 146 1146 624
Fragments arranged by size:
1535 1146 624 310 146
EcoICRI GAG'CTC
Cuts at: 773 773
Size: 3761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 709 1586 2018 2765 3033 517
Size: 192 877 432 747 268 1245
Fragments arranged by size:
1245 877 747 432 268 192
EcoO109I rG'GnC_Cy
Cuts at: 193 223 900 193
Size: 30 677 3054
Fragments arranged by size:
3054 677 30
EcoRI G'AATT_C
Cuts at: 765 765
Size: 3761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1780 1780
Size: 3761
FspI TGC'GCA
Cuts at: 1542 1542
Size: 3761
HaeII r_GCGC'y
Cuts at: 474 1195 1443 2465 3444 474
Size: 721 248 1022 979 791
Fragments arranged by size:
1022 979 791 721 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 279 311 1603 1633 1635 1665 279
Size: 32 1292 30 2 30 2375
Fragments arranged by size:
2375 1292 32 30 30 2
HincII GTy'rAC
Cuts at: 800 800
Size: 3761
KasI G'GCGC_C
Cuts at: 1191 1439 1191
Size: 248 3513
KpnI G_GTAC'C
Cuts at: 781 781
Size: 3761
MluI A'CGCG_T
Cuts at: 3194 3194
Size: 3761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2432 2616 2432
Size: 184 3577
MscI TGG'CCA
Cuts at: 529 1522 529
Size: 993 2768
MslI CAynn'nnrTG
Cuts at: 136 637 1750 1877 136
Size: 501 1113 127 2020
Fragments arranged by size:
2020 1113 501 127
MspA1I CmG'CkG
Cuts at: 872 1186 1546 2559 2804 872
Size: 314 360 1013 245 1829
Fragments arranged by size:
1829 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1942 1942
Size: 3761
NarI GG'CG_CC
Cuts at: 1192 1440 1192
Size: 248 3513
NgoMIV G'CCGG_C
Cuts at: 1940 1940
Size: 3761
NspI r_CATG'y
Cuts at: 607 814 1314 2221 607
Size: 207 500 907 2147
Fragments arranged by size:
2147 907 500 207
PciI A'CATG_T
Cuts at: 2217 2217
Size: 3761
PmeI GTTT'AAAC
Cuts at: 760 760
Size: 3761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2932 2900
Size: 32 3729
PpuMI rG'GwC_Cy
Cuts at: 193 193
Size: 3761
PspOMI G'GGCC_C
Cuts at: 223 223
Size: 3761
PstI C_TGCA'G
Cuts at: 808 1493 808
Size: 685 3076
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3761
PvuII CAG'CTG
Cuts at: 1186 1546 1186
Size: 360 3401
RsrII CG'GwC_CG
Cuts at: 1956 1956
Size: 3761
SacI G_AGCT'C
Cuts at: 775 775
Size: 3761
SalI G'TCGA_C
Cuts at: 798 798
Size: 3761
SapI GCTCTTCn'nnn_
Cuts at: 1784 1994 1784
Size: 210 3551
SbfI CC_TGCA'GG
Cuts at: 808 808
Size: 3761
ScaI AGT'ACT
Cuts at: 3278 3278
Size: 3761
SfcI C'TryA_G
Cuts at: 33 347 804 1489 2482 2673 33
Size: 314 457 685 993 191 1121
Fragments arranged by size:
1121 993 685 457 314 191
SfoI GGC'GCC
Cuts at: 1193 1441 1193
Size: 248 3513
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
SmaI CCC'GGG
Cuts at: 783 783
Size: 3761
SmlI C'TyrA_G
Cuts at: 916 1831 2323 2585 916
Size: 915 492 262 2092
Fragments arranged by size:
2092 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3761
SphI G_CATG'C
Cuts at: 814 1314 814
Size: 500 3261
StyI C'CwwG_G
Cuts at: 749 895 1249 749
Size: 146 354 3261
Fragments arranged by size:
3261 354 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 507 1344 1477 1936 2145 507
Size: 837 133 459 209 2123
Fragments arranged by size:
2123 837 459 209 133
TatI w'GTAC_w
Cuts at: 3276 3276
Size: 3761
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 3729
Tth111I GACn'n_nGTC
Cuts at: 1558 1558
Size: 3761
XbaI T'CTAG_A
Cuts at: 624 792 624
Size: 168 3593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1256 1256
Size: 3761
XmaI C'CCGG_G
Cuts at: 781 781
Size: 3761
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI