(Circular) (Six-base) MAPSORT of: pF1KB6977.seq Check: 6472 from: 1 to: 3764 pF1KB6977 3764 bp With 182 enzymes: SgfI * July 3, 2020 20:21 .. AatII G_ACGT'C Cuts at: 130 130 Size: 3764 AccI GT'mk_AC Cuts at: 802 802 Size: 3764 Acc65I G'GTAC_C Cuts at: 780 780 Size: 3764 AclI AA'CG_TT Cuts at: 3604 3604 Size: 3764 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 666 1589 2021 2768 3036 666 Size: 923 432 747 268 1394 Fragments arranged by size: 1394 923 747 432 268 AflIII A'CryG_T Cuts at: 1116 2220 3197 1116 Size: 1104 977 1683 Fragments arranged by size: 1683 1104 977 AgeI A'CCGG_T Cuts at: 2152 2152 Size: 3764 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 439 439 Size: 3764 AlwNI CAG_nnn'CTG Cuts at: 1069 2636 1069 Size: 1567 2197 ApaLI G'TGCA_C Cuts at: 2534 2534 Size: 3764 ApoI r'AATT_y Cuts at: 768 3117 3373 768 Size: 2349 256 1159 Fragments arranged by size: 2349 1159 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3764 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3764 AvaI C'yCGr_G Cuts at: 784 784 Size: 3764 BamHI G'GATC_C Cuts at: 789 789 Size: 3764 BanI G'GyrC_C Cuts at: 496 780 1194 1442 1477 496 Size: 284 414 248 35 2783 Fragments arranged by size: 2783 414 284 248 35 BanII G_rGCy'C Cuts at: 452 778 1808 452 Size: 326 1030 2408 Fragments arranged by size: 2408 1030 326 BbeI G_GCGC'C Cuts at: 500 1198 1446 500 Size: 698 248 2818 Fragments arranged by size: 2818 698 248 BbsI GAAGACnn'nnnn_ Cuts at: 465 465 Size: 3764 BciVI GTATCCnnnnn_n' Cuts at: 1662 2423 3764 1662 Size: 761 1341 1662 Fragments arranged by size: 1662 1341 761 BclI T'GATC_A Cuts at: 1284 1284 Size: 3764 BglII A'GATC_T Cuts at: 596 1279 3316 596 Size: 683 2037 1044 Fragments arranged by size: 2037 1044 683 BlpI GC'TnA_GC Cuts at: 876 876 Size: 3764 Bme1580I G_kGCm'C Cuts at: 1389 1482 2538 1389 Size: 93 1056 2615 Fragments arranged by size: 2615 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 599 976 1057 1392 3536 599 Size: 377 81 335 2144 827 Fragments arranged by size: 2144 827 377 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2131 2131 Size: 3764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 466 466 Size: 3764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 640 940 1819 2311 2609 640 Size: 300 879 492 298 1795 Fragments arranged by size: 1795 879 492 300 298 BsaI GGTCTCn'nnnn_ Cuts at: 3468 3468 Size: 3764 BsaAI yAC'GTr Cuts at: 1117 1117 Size: 3764 BsaBI GATnn'nnATC Cuts at: 6 1278 3315 6 Size: 1272 2037 455 Fragments arranged by size: 2037 1272 455 BsaHI Gr'CG_yC Cuts at: 127 497 1195 1443 2145 3641 127 Size: 370 698 248 702 1496 250 Fragments arranged by size: 1496 702 698 370 250 248 BsaWI w'CCGG_w Cuts at: 953 1474 2152 2426 2573 2984 953 Size: 521 678 274 147 411 1733 Fragments arranged by size: 1733 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1189 1947 2524 3020 1189 Size: 758 577 496 1933 Fragments arranged by size: 1933 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 229 475 745 229 Size: 246 270 3248 Fragments arranged by size: 3248 270 246 BsiEI CG_ry'CG Cuts at: 104 1306 1352 2560 104 Size: 1202 46 1208 1308 Fragments arranged by size: 1308 1208 1202 46 BsiHKAI G_wGCw'C Cuts at: 778 1556 1746 2538 778 Size: 778 190 792 2004 Fragments arranged by size: 2004 792 778 190 Bsp1286I G_dGCh'C Cuts at: 452 778 1389 1482 1556 1746 1808 2538 452 Size: 326 611 93 74 190 62 730 1678 Fragments arranged by size: 1678 730 611 326 190 93 74 62 BspEI T'CCGG_A Cuts at: 2984 2984 Size: 3764 BspHI T'CATG_A Cuts at: 2940 2940 Size: 3764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 814 1028 1330 1711 814 Size: 214 302 381 2867 Fragments arranged by size: 2867 381 302 214 BsrBI CCG'CTC Cuts at: 50 734 2056 2110 3594 3759 50 Size: 684 1322 54 1484 165 55 Fragments arranged by size: 1484 1322 684 165 55 54 BsrDI GCAATG_nn' Cuts at: 1676 1676 Size: 3764 BsrFI r'CCGG_y Cuts at: 1762 1943 2152 1762 Size: 181 209 3374 Fragments arranged by size: 3374 209 181 BssSI C'ACGA_G Cuts at: 2035 2393 2035 Size: 358 3406 BstAPI GCAn_nnn'nTGC Cuts at: 1141 1141 Size: 3764 BstBI TT'CG_AA Cuts at: 2125 2125 Size: 3764 BstXI CCAn_nnnn'nTGG Cuts at: 486 486 Size: 3764 BstYI r'GATC_y Cuts at: 1 596 789 1256 1279 1614 1860 2872 2958 Size: 595 193 467 23 335 246 1012 86 Cuts at: 2958 2970 3316 1 Size: 12 346 449 Fragments arranged by size: 1012 595 467 449 346 335 246 193 86 23 12 Bsu36I CC'TnA_GG Cuts at: 463 463 Size: 3764 BtgI C'CryG_G Cuts at: 107 107 Size: 3764 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1714 1895 2058 1714 Size: 181 163 3420 Fragments arranged by size: 3420 181 163 BtsI GCAGTG_nn' Cuts at: 186 733 1007 186 Size: 547 274 2943 Fragments arranged by size: 2943 547 274 DraI TTT'AAA Cuts at: 763 763 Size: 3764 DrdI GACnn_nn'nnGTC Cuts at: 511 1470 2328 511 Size: 959 858 1947 Fragments arranged by size: 1947 959 858 EaeI y'GGCC_r Cuts at: 1349 1523 1914 1941 3286 1349 Size: 174 391 27 1345 1827 Fragments arranged by size: 1827 1345 391 174 27 EagI C'GGCC_G Cuts at: 1349 1349 Size: 3764 EarI CTCTTCn'nnn_ Cuts at: 1787 1997 1787 Size: 210 3554 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1972 2282 2428 3574 1972 Size: 310 146 1146 2162 Fragments arranged by size: 2162 1146 310 146 EcoICRI GAG'CTC Cuts at: 776 776 Size: 3764 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 466 666 1589 2021 2768 3036 466 Size: 200 923 432 747 268 1194 Fragments arranged by size: 1194 923 747 432 268 200 EcoNI CCTnn'n_nnAGG Cuts at: 204 372 526 204 Size: 168 154 3442 Fragments arranged by size: 3442 168 154 EcoO109I rG'GnC_Cy Cuts at: 531 903 531 Size: 372 3392 EcoRI G'AATT_C Cuts at: 768 768 Size: 3764 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1783 1783 Size: 3764 FspI TGC'GCA Cuts at: 718 1545 718 Size: 827 2937 HaeII r_GCGC'y Cuts at: 500 1198 1446 2468 3447 500 Size: 698 248 1022 979 817 Fragments arranged by size: 1022 979 817 698 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 616 648 1606 1636 1638 1668 616 Size: 32 958 30 2 30 2712 Fragments arranged by size: 2712 958 32 30 30 2 HincII GTy'rAC Cuts at: 803 803 Size: 3764 KasI G'GCGC_C Cuts at: 496 1194 1442 496 Size: 698 248 2818 Fragments arranged by size: 2818 698 248 KpnI G_GTAC'C Cuts at: 784 784 Size: 3764 MluI A'CGCG_T Cuts at: 3197 3197 Size: 3764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 385 395 422 2435 2619 385 Size: 10 27 2013 184 1530 Fragments arranged by size: 2013 1530 184 27 10 MscI TGG'CCA Cuts at: 1525 1525 Size: 3764 MslI CAynn'nnrTG Cuts at: 641 1753 1880 641 Size: 1112 127 2525 Fragments arranged by size: 2525 1112 127 MspA1I CmG'CkG Cuts at: 252 749 875 1189 1549 2562 2807 252 Size: 497 126 314 360 1013 245 1209 Fragments arranged by size: 1209 1013 497 360 314 245 126 NaeI GCC'GGC Cuts at: 1945 1945 Size: 3764 NarI GG'CG_CC Cuts at: 497 1195 1443 497 Size: 698 248 2818 Fragments arranged by size: 2818 698 248 NcoI C'CATG_G Cuts at: 107 107 Size: 3764 NgoMIV G'CCGG_C Cuts at: 1943 1943 Size: 3764 NspI r_CATG'y Cuts at: 817 1317 2224 817 Size: 500 907 2357 Fragments arranged by size: 2357 907 500 PciI A'CATG_T Cuts at: 2220 2220 Size: 3764 PfoI T'CCnGG_A Cuts at: 121 121 Size: 3764 PmeI GTTT'AAAC Cuts at: 763 763 Size: 3764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2903 2935 2903 Size: 32 3732 PpuMI rG'GwC_Cy Cuts at: 531 531 Size: 3764 PstI C_TGCA'G Cuts at: 323 811 1496 323 Size: 488 685 2591 Fragments arranged by size: 2591 685 488 PvuI CG_AT'CG Cuts at: 104 104 Size: 3764 PvuII CAG'CTG Cuts at: 252 749 1189 1549 252 Size: 497 440 360 2467 Fragments arranged by size: 2467 497 440 360 RsrII CG'GwC_CG Cuts at: 1959 1959 Size: 3764 SacI G_AGCT'C Cuts at: 778 778 Size: 3764 SalI G'TCGA_C Cuts at: 801 801 Size: 3764 SapI GCTCTTCn'nnn_ Cuts at: 1787 1997 1787 Size: 210 3554 SbfI CC_TGCA'GG Cuts at: 811 811 Size: 3764 ScaI AGT'ACT Cuts at: 3281 3281 Size: 3764 SfcI C'TryA_G Cuts at: 33 113 319 612 807 1492 2485 2676 33 Size: 80 206 293 195 685 993 191 1121 Fragments arranged by size: 1121 993 685 293 206 195 191 80 SfoI GGC'GCC Cuts at: 498 1196 1444 498 Size: 698 248 2818 Fragments arranged by size: 2818 698 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3764 SmaI CCC'GGG Cuts at: 786 786 Size: 3764 SmlI C'TyrA_G Cuts at: 655 919 1834 2326 2588 655 Size: 264 915 492 262 1831 Fragments arranged by size: 1831 915 492 264 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3764 SphI G_CATG'C Cuts at: 817 1317 817 Size: 500 3264 StyI C'CwwG_G Cuts at: 107 898 1252 107 Size: 791 354 2619 Fragments arranged by size: 2619 791 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1347 1480 1939 2148 1347 Size: 133 459 209 2963 Fragments arranged by size: 2963 459 209 133 TatI w'GTAC_w Cuts at: 3279 3279 Size: 3764 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3281 3313 3281 Size: 32 3732 Tth111I GACn'n_nGTC Cuts at: 700 1561 700 Size: 861 2903 XbaI T'CTAG_A Cuts at: 795 795 Size: 3764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1259 1259 Size: 3764 XmaI C'CCGG_G Cuts at: 784 784 Size: 3764 ZraI GAC'GTC Cuts at: 128 128 Size: 3764 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI