Restriction map for
pF1KB6979
(Circular) (Six-base) MAPSORT of: pF1KB6979.seq Check: 6787 from: 1 to: 3767
pF1KB6979 3767 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 805 805
Size: 3767
Acc65I G'GTAC_C
Cuts at: 783 783
Size: 3767
AclI AA'CG_TT
Cuts at: 3607 3607
Size: 3767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 702 1592 2024 2771 3039 172
Size: 530 890 432 747 268 900
Fragments arranged by size:
900 890 747 530 432 268
AflIII A'CryG_T
Cuts at: 401 756 1119 2223 3200 401
Size: 355 363 1104 977 968
Fragments arranged by size:
1104 977 968 363 355
AgeI A'CCGG_T
Cuts at: 2155 2155
Size: 3767
AleI CACnn'nnGTG
Cuts at: 490 699 490
Size: 209 3558
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 484 508 484
Size: 24 3743
AlwNI CAG_nnn'CTG
Cuts at: 1072 2639 1072
Size: 1567 2200
ApaI G_GGCC'C
Cuts at: 146 180 146
Size: 34 3733
ApaLI G'TGCA_C
Cuts at: 483 2537 483
Size: 2054 1713
ApoI r'AATT_y
Cuts at: 771 3120 3376 771
Size: 2349 256 1162
Fragments arranged by size:
2349 1162 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3767
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
AvaI C'yCGr_G
Cuts at: 235 371 787 235
Size: 136 416 3215
Fragments arranged by size:
3215 416 136
BamHI G'GATC_C
Cuts at: 560 792 560
Size: 232 3535
BanI G'GyrC_C
Cuts at: 231 255 492 701 783 1197 1445 1480 231
Size: 24 237 209 82 414 248 35 2518
Fragments arranged by size:
2518 414 248 237 209 82 35 24
BanII G_rGCy'C
Cuts at: 146 180 781 1811 146
Size: 34 601 1030 2102
Fragments arranged by size:
2102 1030 601 34
BbeI G_GCGC'C
Cuts at: 1201 1449 1201
Size: 248 3519
BbsI GAAGACnn'nnnn_
Cuts at: 175 175
Size: 3767
BciVI GTATCCnnnnn_n'
Cuts at: 1665 2426 3767 1665
Size: 761 1341 1665
Fragments arranged by size:
1665 1341 761
BclI T'GATC_A
Cuts at: 503 1287 503
Size: 784 2983
BglII A'GATC_T
Cuts at: 1282 3319 1282
Size: 2037 1730
BlpI GC'TnA_GC
Cuts at: 283 879 283
Size: 596 3171
Bme1580I G_kGCm'C
Cuts at: 146 180 487 706 1392 1485 2541 146
Size: 34 307 219 686 93 1056 1372
Fragments arranged by size:
1372 1056 686 307 219 93 34
BmrI ACTGGGnnnn_n'
Cuts at: 435 979 1060 1395 3539 435
Size: 544 81 335 2144 663
Fragments arranged by size:
2144 663 544 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 3767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 705 705
Size: 3767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 457 577 943 1822 2314 2612 280
Size: 177 120 366 879 492 298 1435
Fragments arranged by size:
1435 879 492 366 298 177 120
BsaI GGTCTCn'nnnn_
Cuts at: 3471 3471
Size: 3767
BsaAI yAC'GTr
Cuts at: 757 1120 757
Size: 363 3404
BsaBI GATnn'nnATC
Cuts at: 6 1281 3318 6
Size: 1275 2037 455
Fragments arranged by size:
2037 1275 455
BsaHI Gr'CG_yC
Cuts at: 1198 1446 2148 3644 1198
Size: 248 702 1496 1321
Fragments arranged by size:
1496 1321 702 248
BsaWI w'CCGG_w
Cuts at: 956 1477 2155 2429 2576 2987 956
Size: 521 678 274 147 411 1736
Fragments arranged by size:
1736 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 349 379 535 565 349
Size: 30 156 30 3551
Fragments arranged by size:
3551 156 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 580 590 653 580
Size: 10 63 3694
Fragments arranged by size:
3694 63 10
BseYI C'CCAG_C
Cuts at: 1192 1950 2527 3023 1192
Size: 758 577 496 1936
Fragments arranged by size:
1936 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1309 1355 2563 104
Size: 1205 46 1208 1308
Fragments arranged by size:
1308 1208 1205 46
BsiHKAI G_wGCw'C
Cuts at: 473 487 781 1559 1749 2541 473
Size: 14 294 778 190 792 1699
Fragments arranged by size:
1699 792 778 294 190 14
Bsp1286I G_dGCh'C
Cuts at: 146 180 473 487 706 781 1392 1485 1559
Size: 34 293 14 219 75 611 93 74
Cuts at: 1559 1749 1811 2541 146
Size: 190 62 730 1372
Fragments arranged by size:
1372 730 611 293 219 190 93 75
74 62 34 14
BspEI T'CCGG_A
Cuts at: 2987 2987
Size: 3767
BspHI T'CATG_A
Cuts at: 164 2943 164
Size: 2779 988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 680 817 1031 1333 1714 680
Size: 137 214 302 381 2733
Fragments arranged by size:
2733 381 302 214 137
BsrBI CCG'CTC
Cuts at: 50 546 2059 2113 3597 3762 50
Size: 496 1513 54 1484 165 55
Fragments arranged by size:
1513 1484 496 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1679 1679
Size: 3767
BsrFI r'CCGG_y
Cuts at: 1765 1946 2155 1765
Size: 181 209 3377
Fragments arranged by size:
3377 209 181
BssSI C'ACGA_G
Cuts at: 2038 2396 2038
Size: 358 3409
BstAPI GCAn_nnn'nTGC
Cuts at: 1144 1144
Size: 3767
BstBI TT'CG_AA
Cuts at: 2128 2128
Size: 3767
BstXI CCAn_nnnn'nTGG
Cuts at: 465 666 465
Size: 201 3566
BstYI r'GATC_y
Cuts at: 1 560 792 1259 1282 1617 1863 2875 2961
Size: 559 232 467 23 335 246 1012 86
Cuts at: 2961 2973 3319 1
Size: 12 346 449
Fragments arranged by size:
1012 559 467 449 346 335 246 232
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 260 260
Size: 3767
BtgI C'CryG_G
Cuts at: 107 353 107
Size: 246 3521
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1717 1898 2061 1717
Size: 181 163 3423
Fragments arranged by size:
3423 181 163
BtsI GCAGTG_nn'
Cuts at: 1010 1010
Size: 3767
DraI TTT'AAA
Cuts at: 766 766
Size: 3767
DrdI GACnn_nn'nnGTC
Cuts at: 1473 2331 1473
Size: 858 2909
EaeI y'GGCC_r
Cuts at: 1352 1526 1917 1944 3289 1352
Size: 174 391 27 1345 1830
Fragments arranged by size:
1830 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1352 1352
Size: 3767
EarI CTCTTCn'nnn_
Cuts at: 1790 2000 1790
Size: 210 3557
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1975 2285 2431 3577 1975
Size: 310 146 1146 2165
Fragments arranged by size:
2165 1146 310 146
EcoICRI GAG'CTC
Cuts at: 779 779
Size: 3767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 702 705 1592 2024 2771 3039 172
Size: 530 3 887 432 747 268 900
Fragments arranged by size:
900 887 747 530 432 268 3
EcoO109I rG'GnC_Cy
Cuts at: 142 176 177 477 670 906 142
Size: 34 1 300 193 236 3003
Fragments arranged by size:
3003 300 236 193 34 1
EcoRI G'AATT_C
Cuts at: 771 771
Size: 3767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1786 1786
Size: 3767
FspI TGC'GCA
Cuts at: 1548 1548
Size: 3767
HaeII r_GCGC'y
Cuts at: 1201 1449 2471 3450 1201
Size: 248 1022 979 1518
Fragments arranged by size:
1518 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 276 308 349 381 1609 1639 1641 1671 276
Size: 32 41 32 1228 30 2 30 2372
Fragments arranged by size:
2372 1228 41 32 32 30 30 2
HincII GTy'rAC
Cuts at: 806 806
Size: 3767
KasI G'GCGC_C
Cuts at: 1197 1445 1197
Size: 248 3519
KpnI G_GTAC'C
Cuts at: 787 787
Size: 3767
MluI A'CGCG_T
Cuts at: 3200 3200
Size: 3767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2438 2622 2438
Size: 184 3583
MscI TGG'CCA
Cuts at: 1528 1528
Size: 3767
MslI CAynn'nnrTG
Cuts at: 490 699 1756 1883 490
Size: 209 1057 127 2374
Fragments arranged by size:
2374 1057 209 127
MspA1I CmG'CkG
Cuts at: 397 878 1192 1552 2565 2810 397
Size: 481 314 360 1013 245 1354
Fragments arranged by size:
1354 1013 481 360 314 245
NaeI GCC'GGC
Cuts at: 1948 1948
Size: 3767
NarI GG'CG_CC
Cuts at: 1198 1446 1198
Size: 248 3519
NcoI C'CATG_G
Cuts at: 107 107
Size: 3767
NgoMIV G'CCGG_C
Cuts at: 1946 1946
Size: 3767
NspI r_CATG'y
Cuts at: 405 820 1320 2227 405
Size: 415 500 907 1945
Fragments arranged by size:
1945 907 500 415
PasI CC'CwG_GG
Cuts at: 674 674
Size: 3767
PciI A'CATG_T
Cuts at: 401 2223 401
Size: 1822 1945
PfoI T'CCnGG_A
Cuts at: 200 539 200
Size: 339 3428
PmeI GTTT'AAAC
Cuts at: 766 766
Size: 3767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 2938 2906
Size: 32 3735
PpuMI rG'GwC_Cy
Cuts at: 670 670
Size: 3767
PspOMI G'GGCC_C
Cuts at: 142 176 142
Size: 34 3733
PstI C_TGCA'G
Cuts at: 338 814 1499 338
Size: 476 685 2606
Fragments arranged by size:
2606 685 476
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3767
PvuII CAG'CTG
Cuts at: 397 1192 1552 397
Size: 795 360 2612
Fragments arranged by size:
2612 795 360
RsrII CG'GwC_CG
Cuts at: 1962 1962
Size: 3767
SacI G_AGCT'C
Cuts at: 781 781
Size: 3767
SalI G'TCGA_C
Cuts at: 804 804
Size: 3767
SanDI GG'GwC_CC
Cuts at: 670 670
Size: 3767
SapI GCTCTTCn'nnn_
Cuts at: 1790 2000 1790
Size: 210 3557
SbfI CC_TGCA'GG
Cuts at: 814 814
Size: 3767
ScaI AGT'ACT
Cuts at: 3284 3284
Size: 3767
SfcI C'TryA_G
Cuts at: 33 334 810 1495 2488 2679 33
Size: 301 476 685 993 191 1121
Fragments arranged by size:
1121 993 685 476 301 191
SfoI GGC'GCC
Cuts at: 1199 1447 1199
Size: 248 3519
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
SmaI CCC'GGG
Cuts at: 789 789
Size: 3767
SmlI C'TyrA_G
Cuts at: 295 472 592 922 1837 2329 2591 295
Size: 177 120 330 915 492 262 1471
Fragments arranged by size:
1471 915 492 330 262 177 120
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3767
SphI G_CATG'C
Cuts at: 820 1320 820
Size: 500 3267
StyI C'CwwG_G
Cuts at: 107 488 901 1255 107
Size: 381 413 354 2619
Fragments arranged by size:
2619 413 381 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 570 1350 1483 1942 2151 570
Size: 780 133 459 209 2186
Fragments arranged by size:
2186 780 459 209 133
TatI w'GTAC_w
Cuts at: 3282 3282
Size: 3767
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 3735
Tth111I GACn'n_nGTC
Cuts at: 1564 1564
Size: 3767
XbaI T'CTAG_A
Cuts at: 798 798
Size: 3767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 571 1262 571
Size: 691 3076
XmaI C'CCGG_G
Cuts at: 787 787
Size: 3767
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI