Restriction map for
pF1KB6980
(Circular) (Six-base) MAPSORT of: pF1KB6980.seq Check: 8135 from: 1 to: 3848
pF1KB6980 3848 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 474 474
Size: 3848
AccI GT'mk_AC
Cuts at: 263 886 263
Size: 623 3225
Acc65I G'GTAC_C
Cuts at: 864 864
Size: 3848
AclI AA'CG_TT
Cuts at: 3688 3688
Size: 3848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 1673 2105 2852 3120 333
Size: 1340 432 747 268 1061
Fragments arranged by size:
1340 1061 747 432 268
AflIII A'CryG_T
Cuts at: 1200 2304 3281 1200
Size: 1104 977 1767
Fragments arranged by size:
1767 1104 977
AgeI A'CCGG_T
Cuts at: 2236 2236
Size: 3848
AhdI GACnn_n'nnGTC
Cuts at: 390 390
Size: 3848
AlwNI CAG_nnn'CTG
Cuts at: 1153 2720 1153
Size: 1567 2281
ApaI G_GGCC'C
Cuts at: 318 318
Size: 3848
ApaLI G'TGCA_C
Cuts at: 778 2618 778
Size: 1840 2008
ApoI r'AATT_y
Cuts at: 852 3201 3457 852
Size: 2349 256 1243
Fragments arranged by size:
2349 1243 256
AscI GG'CGCG_CC
Cuts at: 159 159
Size: 3848
AseI AT'TA_AT
Cuts at: 20 20
Size: 3848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 290 296 770 868 290
Size: 6 474 98 3270
Fragments arranged by size:
3270 474 98 6
BamHI G'GATC_C
Cuts at: 873 873
Size: 3848
BanI G'GyrC_C
Cuts at: 864 1278 1526 1561 864
Size: 414 248 35 3151
Fragments arranged by size:
3151 414 248 35
BanII G_rGCy'C
Cuts at: 125 289 297 303 318 862 1892 125
Size: 164 8 6 15 544 1030 2081
Fragments arranged by size:
2081 1030 544 164 15 8 6
BbeI G_GCGC'C
Cuts at: 1282 1530 1282
Size: 248 3600
BbvCI CC'TCA_GC
Cuts at: 625 625
Size: 3848
BciVI GTATCCnnnnn_n'
Cuts at: 551 1746 2507 3848 551
Size: 1195 761 1341 551
Fragments arranged by size:
1341 1195 761 551
BclI T'GATC_A
Cuts at: 1368 1368
Size: 3848
BglI GCCn_nnn'nGGC
Cuts at: 538 538
Size: 3848
BglII A'GATC_T
Cuts at: 1363 3400 1363
Size: 2037 1811
BlpI GC'TnA_GC
Cuts at: 588 960 588
Size: 372 3476
Bme1580I G_kGCm'C
Cuts at: 318 782 1473 1566 2622 318
Size: 464 691 93 1056 1544
Fragments arranged by size:
1544 1056 691 464 93
BmrI ACTGGGnnnn_n'
Cuts at: 1060 1141 1476 3620 1060
Size: 81 335 2144 1288
Fragments arranged by size:
2144 1288 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 3848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 757 139
Size: 618 3230
Bpu10I CC'TnA_GC
Cuts at: 625 625
Size: 3848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 1024 1903 2395 2693 724
Size: 300 879 492 298 1879
Fragments arranged by size:
1879 879 492 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 3552 3552
Size: 3848
BsaAI yAC'GTr
Cuts at: 1201 1201
Size: 3848
BsaBI GATnn'nnATC
Cuts at: 6 472 1362 3399 6
Size: 466 890 2037 455
Fragments arranged by size:
2037 890 466 455
BsaHI Gr'CG_yC
Cuts at: 1279 1527 2229 3725 1279
Size: 248 702 1496 1402
Fragments arranged by size:
1496 1402 702 248
BsaWI w'CCGG_w
Cuts at: 1037 1558 2236 2510 2657 3068 1037
Size: 521 678 274 147 411 1817
Fragments arranged by size:
1817 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 445 475 730 760 112
Size: 30 303 30 255 30 3200
Fragments arranged by size:
3200 303 255 30 30 30
BseYI C'CCAG_C
Cuts at: 656 834 1273 2031 2608 3104 656
Size: 178 439 758 577 496 1400
Fragments arranged by size:
1400 758 577 496 439 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 502 706 436
Size: 66 204 3578
Fragments arranged by size:
3578 204 66
BsiEI CG_ry'CG
Cuts at: 104 1390 1436 2644 104
Size: 1286 46 1208 1308
Fragments arranged by size:
1308 1286 1208 46
BsiHKAI G_wGCw'C
Cuts at: 125 589 782 862 1640 1830 2622 125
Size: 464 193 80 778 190 792 1351
Fragments arranged by size:
1351 792 778 464 193 190 80
BsmI GAATG_Cn'
Cuts at: 531 531
Size: 3848
Bsp1286I G_dGCh'C
Cuts at: 125 289 297 303 318 589 782 862 1473
Size: 164 8 6 15 271 193 80 611
Cuts at: 1473 1566 1640 1830 1892 2622 125
Size: 93 74 190 62 730 1351
Fragments arranged by size:
1351 730 611 271 193 190 164 93
80 74 62 15 8 6
BspEI T'CCGG_A
Cuts at: 3068 3068
Size: 3848
BspHI T'CATG_A
Cuts at: 3024 3024
Size: 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 474 898 1112 1414 1795 474
Size: 424 214 302 381 2527
Fragments arranged by size:
2527 424 381 302 214
BsrBI CCG'CTC
Cuts at: 50 741 2140 2194 3678 3843 50
Size: 691 1399 54 1484 165 55
Fragments arranged by size:
1484 1399 691 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1760 1760
Size: 3848
BsrFI r'CCGG_y
Cuts at: 1846 2027 2236 1846
Size: 181 209 3458
Fragments arranged by size:
3458 209 181
BssHII G'CGCG_C
Cuts at: 159 159
Size: 3848
BssSI C'ACGA_G
Cuts at: 274 724 2119 2477 274
Size: 450 1395 358 1645
Fragments arranged by size:
1645 1395 450 358
BstAPI GCAn_nnn'nTGC
Cuts at: 813 1225 813
Size: 412 3436
BstBI TT'CG_AA
Cuts at: 428 2209 428
Size: 1781 2067
BstXI CCAn_nnnn'nTGG
Cuts at: 252 252
Size: 3848
BstYI r'GATC_y
Cuts at: 1 179 278 873 1340 1363 1698 1944 2956
Size: 178 99 595 467 23 335 246 1012
Cuts at: 2956 3042 3054 3400 1
Size: 86 12 346 449
Fragments arranged by size:
1012 595 467 449 346 335 246 178
99 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 684 684
Size: 3848
BtgI C'CryG_G
Cuts at: 107 107
Size: 3848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 548 1091 548
Size: 543 3305
DraI TTT'AAA
Cuts at: 847 847
Size: 3848
DrdI GACnn_nn'nnGTC
Cuts at: 1554 2412 1554
Size: 858 2990
EaeI y'GGCC_r
Cuts at: 373 1433 1607 1998 2025 3370 373
Size: 1060 174 391 27 1345 851
Fragments arranged by size:
1345 1060 851 391 174 27
EagI C'GGCC_G
Cuts at: 1433 1433
Size: 3848
EarI CTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 406 634 2056 2366 2512 3658 406
Size: 228 1422 310 146 1146 596
Fragments arranged by size:
1422 1146 596 310 228 146
EcoICRI GAG'CTC
Cuts at: 123 860 123
Size: 737 3111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 333 757 1673 2105 2852 3120 139
Size: 194 424 916 432 747 268 867
Fragments arranged by size:
916 867 747 432 424 268 194
EcoO109I rG'GnC_Cy
Cuts at: 314 315 506 987 314
Size: 1 191 481 3175
Fragments arranged by size:
3175 481 191 1
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
EcoRV GAT'ATC
Cuts at: 474 474
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3848
FspI TGC'GCA
Cuts at: 175 1629 175
Size: 1454 2394
HaeII r_GCGC'y
Cuts at: 1282 1530 2552 3531 1282
Size: 248 1022 979 1599
Fragments arranged by size:
1599 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 376 408 1690 1720 1722 1752 112
Size: 32 232 32 1282 30 2 30 2208
Fragments arranged by size:
2208 1282 232 32 32 30 30 2
HincII GTy'rAC
Cuts at: 887 887
Size: 3848
KasI G'GCGC_C
Cuts at: 1278 1526 1278
Size: 248 3600
KpnI G_GTAC'C
Cuts at: 868 868
Size: 3848
MluI A'CGCG_T
Cuts at: 3281 3281
Size: 3848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 110 728 2519 2703 110
Size: 618 1791 184 1255
Fragments arranged by size:
1791 1255 618 184
MscI TGG'CCA
Cuts at: 1609 1609
Size: 3848
MslI CAynn'nnrTG
Cuts at: 815 1837 1964 815
Size: 1022 127 2699
Fragments arranged by size:
2699 1022 127
MspA1I CmG'CkG
Cuts at: 450 592 646 660 959 1273 1633 2646 2891
Size: 142 54 14 299 314 360 1013 245
Cuts at: 2891 450
Size: 1407
Fragments arranged by size:
1407 1013 360 314 299 245 142 54
14
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 3848
NarI GG'CG_CC
Cuts at: 1279 1527 1279
Size: 248 3600
NcoI C'CATG_G
Cuts at: 107 107
Size: 3848
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 3848
NspI r_CATG'y
Cuts at: 814 901 1401 2308 814
Size: 87 500 907 2354
Fragments arranged by size:
2354 907 500 87
PciI A'CATG_T
Cuts at: 2304 2304
Size: 3848
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3816
PpuMI rG'GwC_Cy
Cuts at: 506 506
Size: 3848
PspOMI G'GGCC_C
Cuts at: 314 314
Size: 3848
PspXI vC'TCGA_Gb
Cuts at: 296 296
Size: 3848
PstI C_TGCA'G
Cuts at: 413 895 1580 413
Size: 482 685 2681
Fragments arranged by size:
2681 685 482
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3848
PvuII CAG'CTG
Cuts at: 450 592 646 660 1273 1633 450
Size: 142 54 14 613 360 2665
Fragments arranged by size:
2665 613 360 142 54 14
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 3848
SacI G_AGCT'C
Cuts at: 125 862 125
Size: 737 3111
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
SbfI CC_TGCA'GG
Cuts at: 895 895
Size: 3848
ScaI AGT'ACT
Cuts at: 3365 3365
Size: 3848
SfcI C'TryA_G
Cuts at: 33 409 490 891 1576 2569 2760 33
Size: 376 81 401 685 993 191 1121
Fragments arranged by size:
1121 993 685 401 376 191 81
SfoI GGC'GCC
Cuts at: 1280 1528 1280
Size: 248 3600
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 292 870 292
Size: 578 3270
SmlI C'TyrA_G
Cuts at: 296 703 1003 1918 2410 2672 296
Size: 407 300 915 492 262 1472
Fragments arranged by size:
1472 915 492 407 300 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 901 1401 901
Size: 500 3348
StuI AGG'CCT
Cuts at: 823 823
Size: 3848
StyI C'CwwG_G
Cuts at: 107 245 982 1336 107
Size: 138 737 354 2619
Fragments arranged by size:
2619 737 354 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 470 1431 1564 2023 2232 470
Size: 961 133 459 209 2086
Fragments arranged by size:
2086 961 459 209 133
TatI w'GTAC_w
Cuts at: 3363 3363
Size: 3848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 445 477 3365 3397 445
Size: 32 2888 32 896
Fragments arranged by size:
2888 896 32 32
Tth111I GACn'n_nGTC
Cuts at: 1645 1645
Size: 3848
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1343 114
Size: 1229 2619
XhoI C'TCGA_G
Cuts at: 296 296
Size: 3848
XmaI C'CCGG_G
Cuts at: 290 868 290
Size: 578 3270
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AvrII BaeI
BbsI BcgI BfrBI BmgBI BmtI BseRI BsiWI BsmBI
BsrGI BstEII BstZ17I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI