Restriction map for
pF1KB6982
(Circular) (Six-base) MAPSORT of: pF1KB6982.seq Check: 4960 from: 1 to: 3851
pF1KB6982 3851 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 479 715 889 479
Size: 236 174 3441
Fragments arranged by size:
3441 236 174
Acc65I G'GTAC_C
Cuts at: 867 867
Size: 3851
AclI AA'CG_TT
Cuts at: 3691 3691
Size: 3851
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 1676 2108 2855 3123 728
Size: 948 432 747 268 1456
Fragments arranged by size:
1456 948 747 432 268
AflIII A'CryG_T
Cuts at: 1203 2307 3284 1203
Size: 1104 977 1770
Fragments arranged by size:
1770 1104 977
AgeI A'CCGG_T
Cuts at: 2239 2239
Size: 3851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 723 723
Size: 3851
AlwNI CAG_nnn'CTG
Cuts at: 229 1156 2723 229
Size: 927 1567 1357
Fragments arranged by size:
1567 1357 927
ApaLI G'TGCA_C
Cuts at: 2621 2621
Size: 3851
ApoI r'AATT_y
Cuts at: 664 784 855 3204 3460 664
Size: 120 71 2349 256 1055
Fragments arranged by size:
2349 1055 256 120 71
AseI AT'TA_AT
Cuts at: 20 20
Size: 3851
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
AvaI C'yCGr_G
Cuts at: 160 871 160
Size: 711 3140
BamHI G'GATC_C
Cuts at: 876 876
Size: 3851
BanI G'GyrC_C
Cuts at: 867 1281 1529 1564 867
Size: 414 248 35 3154
Fragments arranged by size:
3154 414 248 35
BanII G_rGCy'C
Cuts at: 865 1895 865
Size: 1030 2821
BbeI G_GCGC'C
Cuts at: 1285 1533 1285
Size: 248 3603
BciVI GTATCCnnnnn_n'
Cuts at: 1749 2510 3851 1749
Size: 761 1341 1749
Fragments arranged by size:
1749 1341 761
BclI T'GATC_A
Cuts at: 1371 1371
Size: 3851
BglII A'GATC_T
Cuts at: 1366 3403 1366
Size: 2037 1814
BlpI GC'TnA_GC
Cuts at: 963 963
Size: 3851
Bme1580I G_kGCm'C
Cuts at: 390 1476 1569 2625 390
Size: 1086 93 1056 1616
Fragments arranged by size:
1616 1086 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1063 1144 1479 3623 1063
Size: 81 335 2144 1291
Fragments arranged by size:
2144 1291 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 3851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1906 2398 2696 1027
Size: 879 492 298 2182
Fragments arranged by size:
2182 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3555 3555
Size: 3851
BsaAI yAC'GTr
Cuts at: 1204 1204
Size: 3851
BsaBI GATnn'nnATC
Cuts at: 6 1365 3402 6
Size: 1359 2037 455
Fragments arranged by size:
2037 1359 455
BsaHI Gr'CG_yC
Cuts at: 1282 1530 2232 3728 1282
Size: 248 702 1496 1405
Fragments arranged by size:
1496 1405 702 248
BsaWI w'CCGG_w
Cuts at: 349 1040 1561 2239 2513 2660 3071 349
Size: 691 521 678 274 147 411 1129
Fragments arranged by size:
1129 691 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1276 2034 2611 3107 1276
Size: 758 577 496 2020
Fragments arranged by size:
2020 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 269
Size: 3851
BsiEI CG_ry'CG
Cuts at: 104 1393 1439 2647 104
Size: 1289 46 1208 1308
Fragments arranged by size:
1308 1289 1208 46
BsiHKAI G_wGCw'C
Cuts at: 865 1643 1833 2625 865
Size: 778 190 792 2091
Fragments arranged by size:
2091 792 778 190
BsmI GAATG_Cn'
Cuts at: 495 495
Size: 3851
BsmBI CGTCTCn'nnnn_
Cuts at: 836 836
Size: 3851
Bsp1286I G_dGCh'C
Cuts at: 390 865 1476 1569 1643 1833 1895 2625 390
Size: 475 611 93 74 190 62 730 1616
Fragments arranged by size:
1616 730 611 475 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3071 3071
Size: 3851
BspHI T'CATG_A
Cuts at: 3027 3027
Size: 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 568 901 1115 1417 1798 568
Size: 333 214 302 381 2621
Fragments arranged by size:
2621 381 333 302 214
BsrBI CCG'CTC
Cuts at: 50 2143 2197 3681 3846 50
Size: 2093 54 1484 165 55
Fragments arranged by size:
2093 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1763 1763
Size: 3851
BsrFI r'CCGG_y
Cuts at: 127 1849 2030 2239 127
Size: 1722 181 209 1739
Fragments arranged by size:
1739 1722 209 181
BssSI C'ACGA_G
Cuts at: 2122 2480 2122
Size: 358 3493
BstAPI GCAn_nnn'nTGC
Cuts at: 1228 1228
Size: 3851
BstBI TT'CG_AA
Cuts at: 2212 2212
Size: 3851
BstYI r'GATC_y
Cuts at: 1 370 876 1343 1366 1701 1947 2959 3045
Size: 369 506 467 23 335 246 1012 86
Cuts at: 3045 3057 3403 1
Size: 12 346 449
Fragments arranged by size:
1012 506 467 449 369 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 554 554
Size: 3851
BtgI C'CryG_G
Cuts at: 114 114
Size: 3851
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1801 1982 2145 1801
Size: 181 163 3507
Fragments arranged by size:
3507 181 163
BtsI GCAGTG_nn'
Cuts at: 602 1094 602
Size: 492 3359
DraI TTT'AAA
Cuts at: 597 850 597
Size: 253 3598
DrdI GACnn_nn'nnGTC
Cuts at: 1557 2415 1557
Size: 858 2993
EaeI y'GGCC_r
Cuts at: 1436 1610 2001 2028 3373 1436
Size: 174 391 27 1345 1914
Fragments arranged by size:
1914 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1436 1436
Size: 3851
EarI CTCTTCn'nnn_
Cuts at: 536 1874 2084 536
Size: 1338 210 2303
Fragments arranged by size:
2303 1338 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2059 2369 2515 3661 2059
Size: 310 146 1146 2249
Fragments arranged by size:
2249 1146 310 146
EcoICRI GAG'CTC
Cuts at: 863 863
Size: 3851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 1676 2108 2855 3123 728
Size: 948 432 747 268 1456
Fragments arranged by size:
1456 948 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 550 990 550
Size: 440 3411
EcoRI G'AATT_C
Cuts at: 855 855
Size: 3851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1870 1870
Size: 3851
FspI TGC'GCA
Cuts at: 1632 1632
Size: 3851
HaeII r_GCGC'y
Cuts at: 128 1285 1533 2555 3534 128
Size: 1157 248 1022 979 445
Fragments arranged by size:
1157 1022 979 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 355 387 801 833 1693 1723 1725 1755 355
Size: 32 414 32 860 30 2 30 2451
Fragments arranged by size:
2451 860 414 32 32 30 30 2
HincII GTy'rAC
Cuts at: 632 890 632
Size: 258 3593
KasI G'GCGC_C
Cuts at: 1281 1529 1281
Size: 248 3603
KpnI G_GTAC'C
Cuts at: 871 871
Size: 3851
MfeI C'AATT_G
Cuts at: 728 728
Size: 3851
MluI A'CGCG_T
Cuts at: 3284 3284
Size: 3851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 738 2522 2706 738
Size: 1784 184 1883
Fragments arranged by size:
1883 1784 184
MscI TGG'CCA
Cuts at: 1612 1612
Size: 3851
MslI CAynn'nnrTG
Cuts at: 1840 1967 1840
Size: 127 3724
MspA1I CmG'CkG
Cuts at: 361 610 962 1276 1636 2649 2894 361
Size: 249 352 314 360 1013 245 1318
Fragments arranged by size:
1318 1013 360 352 314 249 245
NaeI GCC'GGC
Cuts at: 2032 2032
Size: 3851
NarI GG'CG_CC
Cuts at: 1282 1530 1282
Size: 248 3603
NgoMIV G'CCGG_C
Cuts at: 2030 2030
Size: 3851
NspI r_CATG'y
Cuts at: 447 710 904 1404 2311 447
Size: 263 194 500 907 1987
Fragments arranged by size:
1987 907 500 263 194
PciI A'CATG_T
Cuts at: 2307 2307
Size: 3851
PmeI GTTT'AAAC
Cuts at: 850 850
Size: 3851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2990 3022 2990
Size: 32 3819
PstI C_TGCA'G
Cuts at: 579 609 898 1583 579
Size: 30 289 685 2847
Fragments arranged by size:
2847 685 289 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3851
PvuII CAG'CTG
Cuts at: 361 1276 1636 361
Size: 915 360 2576
Fragments arranged by size:
2576 915 360
RsrII CG'GwC_CG
Cuts at: 2046 2046
Size: 3851
SacI G_AGCT'C
Cuts at: 865 865
Size: 3851
SalI G'TCGA_C
Cuts at: 888 888
Size: 3851
SapI GCTCTTCn'nnn_
Cuts at: 536 1874 2084 536
Size: 1338 210 2303
Fragments arranged by size:
2303 1338 210
SbfI CC_TGCA'GG
Cuts at: 579 898 579
Size: 319 3532
ScaI AGT'ACT
Cuts at: 3368 3368
Size: 3851
SfcI C'TryA_G
Cuts at: 33 575 605 894 1579 2572 2763 33
Size: 542 30 289 685 993 191 1121
Fragments arranged by size:
1121 993 685 542 289 191 30
SfoI GGC'GCC
Cuts at: 1283 1531 1283
Size: 248 3603
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
SmaI CCC'GGG
Cuts at: 873 873
Size: 3851
SmlI C'TyrA_G
Cuts at: 1006 1921 2413 2675 1006
Size: 915 492 262 2182
Fragments arranged by size:
2182 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3851
SphI G_CATG'C
Cuts at: 904 1404 904
Size: 500 3351
StuI AGG'CCT
Cuts at: 504 504
Size: 3851
StyI C'CwwG_G
Cuts at: 985 1339 985
Size: 354 3497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1434 1567 2026 2235 1434
Size: 133 459 209 3050
Fragments arranged by size:
3050 459 209 133
TatI w'GTAC_w
Cuts at: 3366 3366
Size: 3851
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 231 263 433 465 3368 3400 231
Size: 32 170 32 2903 32 682
Fragments arranged by size:
2903 682 170 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1648 1648
Size: 3851
XbaI T'CTAG_A
Cuts at: 882 882
Size: 3851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1346 1346
Size: 3851
XmaI C'CCGG_G
Cuts at: 871 871
Size: 3851
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI