Restriction map for
pF1KB6983
(Circular) (Six-base) MAPSORT of: pF1KB6983.seq Check: 9553 from: 1 to: 3767
pF1KB6983 3767 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 805 805
Size: 3767
Acc65I G'GTAC_C
Cuts at: 783 783
Size: 3767
AclI AA'CG_TT
Cuts at: 3607 3607
Size: 3767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1592 2024 2771 3039 1592
Size: 432 747 268 2320
Fragments arranged by size:
2320 747 432 268
AflIII A'CryG_T
Cuts at: 1119 2223 3200 1119
Size: 1104 977 1686
Fragments arranged by size:
1686 1104 977
AgeI A'CCGG_T
Cuts at: 2155 2155
Size: 3767
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 683 715 683
Size: 32 3735
AlwNI CAG_nnn'CTG
Cuts at: 486 1072 2639 486
Size: 586 1567 1614
Fragments arranged by size:
1614 1567 586
ApaI G_GGCC'C
Cuts at: 170 170
Size: 3767
ApaLI G'TGCA_C
Cuts at: 2537 2537
Size: 3767
ApoI r'AATT_y
Cuts at: 771 3120 3376 771
Size: 2349 256 1162
Fragments arranged by size:
2349 1162 256
AseI AT'TA_AT
Cuts at: 20 437 20
Size: 417 3350
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
AvaI C'yCGr_G
Cuts at: 149 169 787 149
Size: 20 618 3129
Fragments arranged by size:
3129 618 20
BamHI G'GATC_C
Cuts at: 792 792
Size: 3767
BanI G'GyrC_C
Cuts at: 589 783 1197 1445 1480 589
Size: 194 414 248 35 2876
Fragments arranged by size:
2876 414 248 194 35
BanII G_rGCy'C
Cuts at: 170 701 712 781 1811 170
Size: 531 11 69 1030 2126
Fragments arranged by size:
2126 1030 531 69 11
BbeI G_GCGC'C
Cuts at: 593 1201 1449 593
Size: 608 248 2911
Fragments arranged by size:
2911 608 248
BciVI GTATCCnnnnn_n'
Cuts at: 1665 2426 3767 1665
Size: 761 1341 1665
Fragments arranged by size:
1665 1341 761
BclI T'GATC_A
Cuts at: 1287 1287
Size: 3767
BglII A'GATC_T
Cuts at: 311 540 1282 3319 311
Size: 229 742 2037 759
Fragments arranged by size:
2037 759 742 229
BlpI GC'TnA_GC
Cuts at: 879 879
Size: 3767
Bme1580I G_kGCm'C
Cuts at: 170 723 1392 1485 2541 170
Size: 553 669 93 1056 1396
Fragments arranged by size:
1396 1056 669 553 93
BmrI ACTGGGnnnn_n'
Cuts at: 710 979 1060 1395 3539 710
Size: 269 81 335 2144 938
Fragments arranged by size:
2144 938 335 269 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 3767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 565
Size: 3767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1822 2314 2612 943
Size: 879 492 298 2098
Fragments arranged by size:
2098 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3471 3471
Size: 3767
BsaAI yAC'GTr
Cuts at: 603 1120 603
Size: 517 3250
BsaBI GATnn'nnATC
Cuts at: 6 1281 3318 6
Size: 1275 2037 455
Fragments arranged by size:
2037 1275 455
BsaHI Gr'CG_yC
Cuts at: 124 590 1198 1446 2148 3644 124
Size: 466 608 248 702 1496 247
Fragments arranged by size:
1496 702 608 466 248 247
BsaWI w'CCGG_w
Cuts at: 677 956 1477 2155 2429 2576 2987 677
Size: 279 521 678 274 147 411 1457
Fragments arranged by size:
1457 678 521 411 279 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 683 713 683
Size: 30 3737
BseYI C'CCAG_C
Cuts at: 184 333 1192 1950 2527 3023 184
Size: 149 859 758 577 496 928
Fragments arranged by size:
928 859 758 577 496 149
BsiEI CG_ry'CG
Cuts at: 104 1309 1355 2563 104
Size: 1205 46 1208 1308
Fragments arranged by size:
1308 1208 1205 46
BsiHKAI G_wGCw'C
Cuts at: 701 781 1559 1749 2541 701
Size: 80 778 190 792 1927
Fragments arranged by size:
1927 792 778 190 80
BsmBI CGTCTCn'nnnn_
Cuts at: 640 640
Size: 3767
Bsp1286I G_dGCh'C
Cuts at: 170 701 712 723 781 1392 1485 1559 1749
Size: 531 11 11 58 611 93 74 190
Cuts at: 1749 1811 2541 170
Size: 62 730 1396
Fragments arranged by size:
1396 730 611 531 190 93 74 62
58 11 11
BspEI T'CCGG_A
Cuts at: 677 2987 677
Size: 2310 1457
BspHI T'CATG_A
Cuts at: 2943 2943
Size: 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 817 1031 1333 1714 817
Size: 214 302 381 2870
Fragments arranged by size:
2870 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2059 2113 3597 3762 50
Size: 2009 54 1484 165 55
Fragments arranged by size:
2009 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1679 1679
Size: 3767
BsrFI r'CCGG_y
Cuts at: 443 1765 1946 2155 443
Size: 1322 181 209 2055
Fragments arranged by size:
2055 1322 209 181
BssHII G'CGCG_C
Cuts at: 236 236
Size: 3767
BssSI C'ACGA_G
Cuts at: 2038 2396 2038
Size: 358 3409
BstAPI GCAn_nnn'nTGC
Cuts at: 399 486 666 1144 399
Size: 87 180 478 3022
Fragments arranged by size:
3022 478 180 87
BstBI TT'CG_AA
Cuts at: 2128 2128
Size: 3767
BstYI r'GATC_y
Cuts at: 1 311 540 792 1259 1282 1617 1863 2875
Size: 310 229 252 467 23 335 246 1012
Cuts at: 2875 2961 2973 3319 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 310 252 246
229 86 23 12
BtgI C'CryG_G
Cuts at: 359 411 359
Size: 52 3715
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1717 1898 2061 1717
Size: 181 163 3423
Fragments arranged by size:
3423 181 163
BtsI GCAGTG_nn'
Cuts at: 324 1010 324
Size: 686 3081
DraI TTT'AAA
Cuts at: 766 766
Size: 3767
DraIII CAC_nnn'GTG
Cuts at: 603 603
Size: 3767
DrdI GACnn_nn'nnGTC
Cuts at: 1473 2331 1473
Size: 858 2909
EaeI y'GGCC_r
Cuts at: 408 623 1352 1526 1917 1944 3289 408
Size: 215 729 174 391 27 1345 886
Fragments arranged by size:
1345 886 729 391 215 174 27
EagI C'GGCC_G
Cuts at: 1352 1352
Size: 3767
EarI CTCTTCn'nnn_
Cuts at: 365 478 1790 2000 365
Size: 113 1312 210 2132
Fragments arranged by size:
2132 1312 210 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1975 2285 2431 3577 1975
Size: 310 146 1146 2165
Fragments arranged by size:
2165 1146 310 146
EcoICRI GAG'CTC
Cuts at: 699 779 699
Size: 80 3687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1592 2024 2771 3039 565
Size: 1027 432 747 268 1293
Fragments arranged by size:
1293 1027 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 160 174 561 906 160
Size: 14 387 345 3021
Fragments arranged by size:
3021 387 345 14
EcoRI G'AATT_C
Cuts at: 771 771
Size: 3767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1786 1786
Size: 3767
FspI TGC'GCA
Cuts at: 1548 1548
Size: 3767
HaeII r_GCGC'y
Cuts at: 558 593 1201 1449 2471 3450 558
Size: 35 608 248 1022 979 875
Fragments arranged by size:
1022 979 875 608 248 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 532 564 1609 1639 1641 1671 532
Size: 32 1045 30 2 30 2628
Fragments arranged by size:
2628 1045 32 30 30 2
HincII GTy'rAC
Cuts at: 806 806
Size: 3767
KasI G'GCGC_C
Cuts at: 589 1197 1445 589
Size: 608 248 2911
Fragments arranged by size:
2911 608 248
KpnI G_GTAC'C
Cuts at: 787 787
Size: 3767
MluI A'CGCG_T
Cuts at: 3200 3200
Size: 3767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2438 2622 2438
Size: 184 3583
MscI TGG'CCA
Cuts at: 410 1528 410
Size: 1118 2649
MslI CAynn'nnrTG
Cuts at: 1756 1883 1756
Size: 127 3640
MspA1I CmG'CkG
Cuts at: 116 361 663 878 1192 1552 2565 2810 116
Size: 245 302 215 314 360 1013 245 1073
Fragments arranged by size:
1073 1013 360 314 302 245 245 215
NaeI GCC'GGC
Cuts at: 1948 1948
Size: 3767
NarI GG'CG_CC
Cuts at: 590 1198 1446 590
Size: 608 248 2911
Fragments arranged by size:
2911 608 248
NgoMIV G'CCGG_C
Cuts at: 1946 1946
Size: 3767
NspI r_CATG'y
Cuts at: 402 820 1320 2227 402
Size: 418 500 907 1942
Fragments arranged by size:
1942 907 500 418
PciI A'CATG_T
Cuts at: 2223 2223
Size: 3767
PflMI CCAn_nnn'nTGG
Cuts at: 603 603
Size: 3767
PfoI T'CCnGG_A
Cuts at: 127 350 127
Size: 223 3544
PmeI GTTT'AAAC
Cuts at: 766 766
Size: 3767
PmlI CAC'GTG
Cuts at: 603 603
Size: 3767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 683 715 2906 2938 683
Size: 32 2191 32 1512
Fragments arranged by size:
2191 1512 32 32
PpuMI rG'GwC_Cy
Cuts at: 160 174 561 160
Size: 14 387 3366
Fragments arranged by size:
3366 387 14
PspOMI G'GGCC_C
Cuts at: 166 166
Size: 3767
PstI C_TGCA'G
Cuts at: 319 814 1499 319
Size: 495 685 2587
Fragments arranged by size:
2587 685 495
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3767
PvuII CAG'CTG
Cuts at: 663 1192 1552 663
Size: 529 360 2878
Fragments arranged by size:
2878 529 360
RsrII CG'GwC_CG
Cuts at: 1962 1962
Size: 3767
SacI G_AGCT'C
Cuts at: 701 781 701
Size: 80 3687
SacII CC_GC'GG
Cuts at: 362 362
Size: 3767
SalI G'TCGA_C
Cuts at: 804 804
Size: 3767
SanDI GG'GwC_CC
Cuts at: 174 174
Size: 3767
SapI GCTCTTCn'nnn_
Cuts at: 478 1790 2000 478
Size: 1312 210 2245
Fragments arranged by size:
2245 1312 210
SbfI CC_TGCA'GG
Cuts at: 814 814
Size: 3767
ScaI AGT'ACT
Cuts at: 3284 3284
Size: 3767
SfcI C'TryA_G
Cuts at: 33 315 810 1495 2488 2679 33
Size: 282 495 685 993 191 1121
Fragments arranged by size:
1121 993 685 495 282 191
SfoI GGC'GCC
Cuts at: 591 1199 1447 591
Size: 608 248 2911
Fragments arranged by size:
2911 608 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
SmaI CCC'GGG
Cuts at: 151 171 789 151
Size: 20 618 3129
Fragments arranged by size:
3129 618 20
SmlI C'TyrA_G
Cuts at: 922 1837 2329 2591 922
Size: 915 492 262 2098
Fragments arranged by size:
2098 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3767
SphI G_CATG'C
Cuts at: 820 1320 820
Size: 500 3267
StuI AGG'CCT
Cuts at: 619 619
Size: 3767
StyI C'CwwG_G
Cuts at: 901 1255 901
Size: 354 3413
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1350 1483 1942 2151 1350
Size: 133 459 209 2966
Fragments arranged by size:
2966 459 209 133
TatI w'GTAC_w
Cuts at: 395 3282 395
Size: 2887 880
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 3735
Tth111I GACn'n_nGTC
Cuts at: 1564 1564
Size: 3767
XbaI T'CTAG_A
Cuts at: 798 798
Size: 3767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1262 1262
Size: 3767
XmaI C'CCGG_G
Cuts at: 149 169 787 149
Size: 20 618 3129
Fragments arranged by size:
3129 618 20
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmI BsrGI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PshAI PsiI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI