Restriction map for
pF1KB6984
(Circular) (Six-base) MAPSORT of: pF1KB6984.seq Check: 2826 from: 1 to: 3854
pF1KB6984 3854 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 791 803 892 791
Size: 12 89 3753
Fragments arranged by size:
3753 89 12
Acc65I G'GTAC_C
Cuts at: 870 870
Size: 3854
AclI AA'CG_TT
Cuts at: 3694 3694
Size: 3854
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1679 2111 2858 3126 1679
Size: 432 747 268 2407
Fragments arranged by size:
2407 747 432 268
AflIII A'CryG_T
Cuts at: 653 1206 2310 3287 653
Size: 553 1104 977 1220
Fragments arranged by size:
1220 1104 977 553
AgeI A'CCGG_T
Cuts at: 2242 2242
Size: 3854
AleI CACnn'nnGTG
Cuts at: 248 248
Size: 3854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 312 312
Size: 3854
AlwNI CAG_nnn'CTG
Cuts at: 511 588 1159 2726 511
Size: 77 571 1567 1639
Fragments arranged by size:
1639 1567 571 77
ApaLI G'TGCA_C
Cuts at: 2624 2624
Size: 3854
ApoI r'AATT_y
Cuts at: 204 231 858 3207 3463 204
Size: 27 627 2349 256 595
Fragments arranged by size:
2349 627 595 256 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 3854
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
AvaI C'yCGr_G
Cuts at: 874 874
Size: 3854
BamHI G'GATC_C
Cuts at: 879 879
Size: 3854
BanI G'GyrC_C
Cuts at: 559 870 1284 1532 1567 559
Size: 311 414 248 35 2846
Fragments arranged by size:
2846 414 311 248 35
BanII G_rGCy'C
Cuts at: 556 868 1898 556
Size: 312 1030 2512
Fragments arranged by size:
2512 1030 312
BbeI G_GCGC'C
Cuts at: 563 1288 1536 563
Size: 725 248 2881
Fragments arranged by size:
2881 725 248
BbsI GAAGACnn'nnnn_
Cuts at: 418 418
Size: 3854
BciVI GTATCCnnnnn_n'
Cuts at: 1752 2513 3854 1752
Size: 761 1341 1752
Fragments arranged by size:
1752 1341 761
BclI T'GATC_A
Cuts at: 1374 1374
Size: 3854
BglII A'GATC_T
Cuts at: 1369 3406 1369
Size: 2037 1817
BlpI GC'TnA_GC
Cuts at: 604 966 604
Size: 362 3492
Bme1580I G_kGCm'C
Cuts at: 771 1479 1572 2628 771
Size: 708 93 1056 1997
Fragments arranged by size:
1997 1056 708 93
BmrI ACTGGGnnnn_n'
Cuts at: 334 1066 1147 1482 3626 334
Size: 732 81 335 2144 562
Fragments arranged by size:
2144 732 562 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 3854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 488 806 370
Size: 118 318 3418
Fragments arranged by size:
3418 318 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1030 1909 2401 2699 694
Size: 336 879 492 298 1849
Fragments arranged by size:
1849 879 492 336 298
BsaI GGTCTCn'nnnn_
Cuts at: 3558 3558
Size: 3854
BsaAI yAC'GTr
Cuts at: 454 1207 454
Size: 753 3101
BsaBI GATnn'nnATC
Cuts at: 6 1368 3405 6
Size: 1362 2037 455
Fragments arranged by size:
2037 1362 455
BsaHI Gr'CG_yC
Cuts at: 560 1285 1533 2235 3731 560
Size: 725 248 702 1496 683
Fragments arranged by size:
1496 725 702 683 248
BsaWI w'CCGG_w
Cuts at: 1043 1564 2242 2516 2663 3074 1043
Size: 521 678 274 147 411 1823
Fragments arranged by size:
1823 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 486 516 486
Size: 30 3824
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 438 214
Size: 224 3630
BseYI C'CCAG_C
Cuts at: 536 1279 2037 2614 3110 536
Size: 743 758 577 496 1280
Fragments arranged by size:
1280 758 743 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 476
Size: 3854
BsiEI CG_ry'CG
Cuts at: 104 1396 1442 2650 104
Size: 1292 46 1208 1308
Fragments arranged by size:
1308 1292 1208 46
BsiHKAI G_wGCw'C
Cuts at: 335 556 868 1646 1836 2628 335
Size: 221 312 778 190 792 1561
Fragments arranged by size:
1561 792 778 312 221 190
Bsp1286I G_dGCh'C
Cuts at: 335 556 771 868 1479 1572 1646 1836 1898
Size: 221 215 97 611 93 74 190 62
Cuts at: 1898 2628 335
Size: 730 1561
Fragments arranged by size:
1561 730 611 221 215 190 97 93
74 62
BspEI T'CCGG_A
Cuts at: 3074 3074
Size: 3854
BspHI T'CATG_A
Cuts at: 3030 3030
Size: 3854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 904 1118 1420 1801 904
Size: 214 302 381 2957
Fragments arranged by size:
2957 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2146 2200 3684 3849 50
Size: 2096 54 1484 165 55
Fragments arranged by size:
2096 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 732 1766 732
Size: 1034 2820
BsrFI r'CCGG_y
Cuts at: 1852 2033 2242 1852
Size: 181 209 3464
Fragments arranged by size:
3464 209 181
BssSI C'ACGA_G
Cuts at: 2125 2483 2125
Size: 358 3496
BstAPI GCAn_nnn'nTGC
Cuts at: 521 1231 521
Size: 710 3144
BstBI TT'CG_AA
Cuts at: 2215 2215
Size: 3854
BstXI CCAn_nnnn'nTGG
Cuts at: 250 250
Size: 3854
BstYI r'GATC_y
Cuts at: 1 879 1346 1369 1704 1950 2962 3048 3060
Size: 878 467 23 335 246 1012 86 12
Cuts at: 3060 3406 1
Size: 346 449
Fragments arranged by size:
1012 878 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1804 1985 2148 1804
Size: 181 163 3510
Fragments arranged by size:
3510 181 163
BtsI GCAGTG_nn'
Cuts at: 294 545 589 1097 294
Size: 251 44 508 3051
Fragments arranged by size:
3051 508 251 44
DraI TTT'AAA
Cuts at: 853 853
Size: 3854
DraIII CAC_nnn'GTG
Cuts at: 521 521
Size: 3854
DrdI GACnn_nn'nnGTC
Cuts at: 1560 2418 1560
Size: 858 2996
EaeI y'GGCC_r
Cuts at: 1439 1613 2004 2031 3376 1439
Size: 174 391 27 1345 1917
Fragments arranged by size:
1917 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1439 1439
Size: 3854
EarI CTCTTCn'nnn_
Cuts at: 1877 2087 1877
Size: 210 3644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 379 2062 2372 2518 3664 379
Size: 1683 310 146 1146 569
Fragments arranged by size:
1683 1146 569 310 146
EcoICRI GAG'CTC
Cuts at: 554 866 554
Size: 312 3542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 488 806 1679 2111 2858 3126 370
Size: 118 318 873 432 747 268 1098
Fragments arranged by size:
1098 873 747 432 318 268 118
EcoO109I rG'GnC_Cy
Cuts at: 993 993
Size: 3854
EcoRI G'AATT_C
Cuts at: 204 231 858 204
Size: 27 627 3200
Fragments arranged by size:
3200 627 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1873 1873
Size: 3854
FspI TGC'GCA
Cuts at: 1635 1635
Size: 3854
HaeII r_GCGC'y
Cuts at: 563 1288 1536 2558 3537 563
Size: 725 248 1022 979 880
Fragments arranged by size:
1022 979 880 725 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1696 1726 1728 1758 1696
Size: 30 2 30 3792
Fragments arranged by size:
3792 30 30 2
HincII GTy'rAC
Cuts at: 893 893
Size: 3854
KasI G'GCGC_C
Cuts at: 559 1284 1532 559
Size: 725 248 2881
Fragments arranged by size:
2881 725 248
KpnI G_GTAC'C
Cuts at: 874 874
Size: 3854
MluI A'CGCG_T
Cuts at: 3287 3287
Size: 3854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2525 2709 2525
Size: 184 3670
MscI TGG'CCA
Cuts at: 1615 1615
Size: 3854
MslI CAynn'nnrTG
Cuts at: 248 316 1843 1970 248
Size: 68 1527 127 2132
Fragments arranged by size:
2132 1527 127 68
MspA1I CmG'CkG
Cuts at: 142 965 1279 1639 2652 2897 142
Size: 823 314 360 1013 245 1099
Fragments arranged by size:
1099 1013 823 360 314 245
NaeI GCC'GGC
Cuts at: 2035 2035
Size: 3854
NarI GG'CG_CC
Cuts at: 560 1285 1533 560
Size: 725 248 2881
Fragments arranged by size:
2881 725 248
NgoMIV G'CCGG_C
Cuts at: 2033 2033
Size: 3854
NspI r_CATG'y
Cuts at: 657 907 1407 2314 657
Size: 250 500 907 2197
Fragments arranged by size:
2197 907 500 250
PciI A'CATG_T
Cuts at: 653 2310 653
Size: 1657 2197
PflMI CCAn_nnn'nTGG
Cuts at: 511 511
Size: 3854
PfoI T'CCnGG_A
Cuts at: 347 347
Size: 3854
PmeI GTTT'AAAC
Cuts at: 853 853
Size: 3854
PmlI CAC'GTG
Cuts at: 454 454
Size: 3854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2993 3025 2993
Size: 32 3822
PstI C_TGCA'G
Cuts at: 584 901 1586 584
Size: 317 685 2852
Fragments arranged by size:
2852 685 317
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3854
PvuII CAG'CTG
Cuts at: 142 1279 1639 142
Size: 1137 360 2357
Fragments arranged by size:
2357 1137 360
RsrII CG'GwC_CG
Cuts at: 2049 2049
Size: 3854
SacI G_AGCT'C
Cuts at: 556 868 556
Size: 312 3542
SalI G'TCGA_C
Cuts at: 891 891
Size: 3854
SapI GCTCTTCn'nnn_
Cuts at: 1877 2087 1877
Size: 210 3644
SbfI CC_TGCA'GG
Cuts at: 901 901
Size: 3854
ScaI AGT'ACT
Cuts at: 3371 3371
Size: 3854
SexAI A'CCwGG_T
Cuts at: 218 218
Size: 3854
SfcI C'TryA_G
Cuts at: 33 580 897 1582 2575 2766 33
Size: 547 317 685 993 191 1121
Fragments arranged by size:
1121 993 685 547 317 191
SfoI GGC'GCC
Cuts at: 561 1286 1534 561
Size: 725 248 2881
Fragments arranged by size:
2881 725 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3854
SmaI CCC'GGG
Cuts at: 876 876
Size: 3854
SmlI C'TyrA_G
Cuts at: 673 1009 1924 2416 2678 673
Size: 336 915 492 262 1849
Fragments arranged by size:
1849 915 492 336 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3854
SphI G_CATG'C
Cuts at: 907 1407 907
Size: 500 3354
StyI C'CwwG_G
Cuts at: 738 797 988 1342 738
Size: 59 191 354 3250
Fragments arranged by size:
3250 354 191 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 267 1437 1570 2029 2238 267
Size: 1170 133 459 209 1883
Fragments arranged by size:
1883 1170 459 209 133
TatI w'GTAC_w
Cuts at: 3369 3369
Size: 3854
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 107 139 486 518 3371 3403 107
Size: 32 347 32 2853 32 558
Fragments arranged by size:
2853 558 347 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1651 1651
Size: 3854
XbaI T'CTAG_A
Cuts at: 885 885
Size: 3854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 250 1349 250
Size: 1099 2755
XmaI C'CCGG_G
Cuts at: 874 874
Size: 3854
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI