Restriction map for
pF1KB6986
(Circular) (Six-base) MAPSORT of: pF1KB6986.seq Check: 1168 from: 1 to: 3857
pF1KB6986 3857 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 895 895
Size: 3857
Acc65I G'GTAC_C
Cuts at: 873 873
Size: 3857
AclI AA'CG_TT
Cuts at: 3697 3697
Size: 3857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 794 1682 2114 2861 3129 469
Size: 325 888 432 747 268 1197
Fragments arranged by size:
1197 888 747 432 325 268
AflIII A'CryG_T
Cuts at: 1209 2313 3290 1209
Size: 1104 977 1776
Fragments arranged by size:
1776 1104 977
AgeI A'CCGG_T
Cuts at: 2245 2245
Size: 3857
AlwNI CAG_nnn'CTG
Cuts at: 1162 2729 1162
Size: 1567 2290
ApaI G_GGCC'C
Cuts at: 369 369
Size: 3857
ApaLI G'TGCA_C
Cuts at: 2627 2627
Size: 3857
ApoI r'AATT_y
Cuts at: 861 3210 3466 861
Size: 2349 256 1252
Fragments arranged by size:
2349 1252 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3857
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
AvaI C'yCGr_G
Cuts at: 368 555 877 368
Size: 187 322 3348
Fragments arranged by size:
3348 322 187
AvrII C'CTAG_G
Cuts at: 532 532
Size: 3857
BamHI G'GATC_C
Cuts at: 882 882
Size: 3857
BanI G'GyrC_C
Cuts at: 598 873 1287 1535 1570 598
Size: 275 414 248 35 2885
Fragments arranged by size:
2885 414 275 248 35
BanII G_rGCy'C
Cuts at: 196 369 871 1901 196
Size: 173 502 1030 2152
Fragments arranged by size:
2152 1030 502 173
BbeI G_GCGC'C
Cuts at: 1291 1539 1291
Size: 248 3609
BbsI GAAGACnn'nnnn_
Cuts at: 126 126
Size: 3857
BciVI GTATCCnnnnn_n'
Cuts at: 1755 2516 3857 1755
Size: 761 1341 1755
Fragments arranged by size:
1755 1341 761
BclI T'GATC_A
Cuts at: 680 1377 680
Size: 697 3160
BglI GCCn_nnn'nGGC
Cuts at: 577 577
Size: 3857
BglII A'GATC_T
Cuts at: 290 1372 3409 290
Size: 1082 2037 738
Fragments arranged by size:
2037 1082 738
BlpI GC'TnA_GC
Cuts at: 969 969
Size: 3857
Bme1580I G_kGCm'C
Cuts at: 369 562 1482 1575 2631 369
Size: 193 920 93 1056 1595
Fragments arranged by size:
1595 1056 920 193 93
BmrI ACTGGGnnnn_n'
Cuts at: 1069 1150 1485 3629 1069
Size: 81 335 2144 1297
Fragments arranged by size:
2144 1297 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 3857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 643
Size: 3857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1033 1912 2404 2702 742
Size: 291 879 492 298 1897
Fragments arranged by size:
1897 879 492 298 291
BsaI GGTCTCn'nnnn_
Cuts at: 3561 3561
Size: 3857
BsaAI yAC'GTr
Cuts at: 1210 1210
Size: 3857
BsaBI GATnn'nnATC
Cuts at: 6 355 1371 3408 6
Size: 349 1016 2037 455
Fragments arranged by size:
2037 1016 455 349
BsaHI Gr'CG_yC
Cuts at: 1288 1536 2238 3734 1288
Size: 248 702 1496 1411
Fragments arranged by size:
1496 1411 702 248
BsaWI w'CCGG_w
Cuts at: 1046 1567 2245 2519 2666 3077 1046
Size: 521 678 274 147 411 1826
Fragments arranged by size:
1826 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 235 265 235
Size: 30 3827
BseYI C'CCAG_C
Cuts at: 1282 2040 2617 3113 1282
Size: 758 577 496 2026
Fragments arranged by size:
2026 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 120 200 1399 1445 2653 104
Size: 16 80 1199 46 1208 1308
Fragments arranged by size:
1308 1208 1199 80 46 16
BsiHKAI G_wGCw'C
Cuts at: 871 1649 1839 2631 871
Size: 778 190 792 2097
Fragments arranged by size:
2097 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 196 369 562 871 1482 1575 1649 1839 1901
Size: 173 193 309 611 93 74 190 62
Cuts at: 1901 2631 196
Size: 730 1422
Fragments arranged by size:
1422 730 611 309 193 190 173 93
74 62
BspEI T'CCGG_A
Cuts at: 3077 3077
Size: 3857
BspHI T'CATG_A
Cuts at: 737 3033 737
Size: 2296 1561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 907 1121 1423 1804 907
Size: 214 302 381 2960
Fragments arranged by size:
2960 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2149 2203 3687 3852 50
Size: 2099 54 1484 165 55
Fragments arranged by size:
2099 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 477 1769 477
Size: 1292 2565
BsrFI r'CCGG_y
Cuts at: 389 1855 2036 2245 389
Size: 1466 181 209 2001
Fragments arranged by size:
2001 1466 209 181
BssSI C'ACGA_G
Cuts at: 2128 2486 2128
Size: 358 3499
BstAPI GCAn_nnn'nTGC
Cuts at: 1234 1234
Size: 3857
BstBI TT'CG_AA
Cuts at: 2218 2218
Size: 3857
BstXI CCAn_nnnn'nTGG
Cuts at: 675 675
Size: 3857
BstYI r'GATC_y
Cuts at: 1 290 882 1349 1372 1707 1953 2965 3051
Size: 289 592 467 23 335 246 1012 86
Cuts at: 3051 3063 3409 1
Size: 12 346 449
Fragments arranged by size:
1012 592 467 449 346 335 289 246
86 23 12
BtgI C'CryG_G
Cuts at: 200 200
Size: 3857
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 423 1807 1988 2151 423
Size: 1384 181 163 2129
Fragments arranged by size:
2129 1384 181 163
BtsI GCAGTG_nn'
Cuts at: 1100 1100
Size: 3857
DraI TTT'AAA
Cuts at: 767 856 767
Size: 89 3768
DrdI GACnn_nn'nnGTC
Cuts at: 1563 2421 1563
Size: 858 2999
EaeI y'GGCC_r
Cuts at: 147 257 1442 1616 2007 2034 3379 147
Size: 110 1185 174 391 27 1345 625
Fragments arranged by size:
1345 1185 625 391 174 110 27
EagI C'GGCC_G
Cuts at: 1442 1442
Size: 3857
EarI CTCTTCn'nnn_
Cuts at: 160 213 1880 2090 160
Size: 53 1667 210 1927
Fragments arranged by size:
1927 1667 210 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2065 2375 2521 3667 2065
Size: 310 146 1146 2255
Fragments arranged by size:
2255 1146 310 146
EcoICRI GAG'CTC
Cuts at: 869 869
Size: 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 643 794 1682 2114 2861 3129 469
Size: 174 151 888 432 747 268 1197
Fragments arranged by size:
1197 888 747 432 268 174 151
EcoO109I rG'GnC_Cy
Cuts at: 365 405 996 365
Size: 40 591 3226
Fragments arranged by size:
3226 591 40
EcoRI G'AATT_C
Cuts at: 861 861
Size: 3857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1876 1876
Size: 3857
FspI TGC'GCA
Cuts at: 1638 1638
Size: 3857
HaeII r_GCGC'y
Cuts at: 1291 1539 2561 3540 1291
Size: 248 1022 979 1608
Fragments arranged by size:
1608 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 267 732 753 764 785 1699 1729 1731
Size: 32 465 21 11 21 914 30 2
Cuts at: 1731 1761 235
Size: 30 2331
Fragments arranged by size:
2331 914 465 32 30 30 21 21
11 2
HincII GTy'rAC
Cuts at: 896 896
Size: 3857
KasI G'GCGC_C
Cuts at: 1287 1535 1287
Size: 248 3609
KpnI G_GTAC'C
Cuts at: 877 877
Size: 3857
MluI A'CGCG_T
Cuts at: 3290 3290
Size: 3857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2528 2712 2528
Size: 184 3673
MscI TGG'CCA
Cuts at: 259 1618 259
Size: 1359 2498
MslI CAynn'nnrTG
Cuts at: 1846 1973 1846
Size: 127 3730
MspA1I CmG'CkG
Cuts at: 202 968 1282 1642 2655 2900 202
Size: 766 314 360 1013 245 1159
Fragments arranged by size:
1159 1013 766 360 314 245
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 3857
NarI GG'CG_CC
Cuts at: 1288 1536 1288
Size: 248 3609
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 3857
NspI r_CATG'y
Cuts at: 910 1410 2317 910
Size: 500 907 2450
Fragments arranged by size:
2450 907 500
PciI A'CATG_T
Cuts at: 2313 2313
Size: 3857
PfoI T'CCnGG_A
Cuts at: 620 692 620
Size: 72 3785
PmeI GTTT'AAAC
Cuts at: 856 856
Size: 3857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3825
PpuMI rG'GwC_Cy
Cuts at: 405 405
Size: 3857
PspOMI G'GGCC_C
Cuts at: 365 365
Size: 3857
PstI C_TGCA'G
Cuts at: 269 904 1589 269
Size: 635 685 2537
Fragments arranged by size:
2537 685 635
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3857
PvuII CAG'CTG
Cuts at: 1282 1642 1282
Size: 360 3497
RsrII CG'GwC_CG
Cuts at: 2052 2052
Size: 3857
SacI G_AGCT'C
Cuts at: 871 871
Size: 3857
SacII CC_GC'GG
Cuts at: 203 203
Size: 3857
SalI G'TCGA_C
Cuts at: 894 894
Size: 3857
SapI GCTCTTCn'nnn_
Cuts at: 1880 2090 1880
Size: 210 3647
SbfI CC_TGCA'GG
Cuts at: 904 904
Size: 3857
ScaI AGT'ACT
Cuts at: 3374 3374
Size: 3857
SfcI C'TryA_G
Cuts at: 33 265 900 1585 2578 2769 33
Size: 232 635 685 993 191 1121
Fragments arranged by size:
1121 993 685 635 232 191
SfoI GGC'GCC
Cuts at: 1289 1537 1289
Size: 248 3609
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
SmaI CCC'GGG
Cuts at: 370 879 370
Size: 509 3348
SmlI C'TyrA_G
Cuts at: 757 1012 1927 2419 2681 757
Size: 255 915 492 262 1933
Fragments arranged by size:
1933 915 492 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3857
SphI G_CATG'C
Cuts at: 910 1410 910
Size: 500 3357
StuI AGG'CCT
Cuts at: 332 332
Size: 3857
StyI C'CwwG_G
Cuts at: 532 991 1345 532
Size: 459 354 3044
Fragments arranged by size:
3044 459 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1440 1573 2032 2241 1440
Size: 133 459 209 3056
Fragments arranged by size:
3056 459 209 133
TatI w'GTAC_w
Cuts at: 422 3372 422
Size: 2950 907
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 279 311 3374 3406 279
Size: 32 3063 32 730
Fragments arranged by size:
3063 730 32 32
Tth111I GACn'n_nGTC
Cuts at: 310 409 1654 310
Size: 99 1245 2513
Fragments arranged by size:
2513 1245 99
XbaI T'CTAG_A
Cuts at: 888 888
Size: 3857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1352 1352
Size: 3857
XmaI C'CCGG_G
Cuts at: 368 877 368
Size: 509 3348
XmnI GAAnn'nnTTC
Cuts at: 701 701
Size: 3857
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI