Restriction map for
pF1KB6988
(Circular) (Six-base) MAPSORT of: pF1KB6988.seq Check: 8381 from: 1 to: 3857
pF1KB6988 3857 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AatII G_ACGT'C
Cuts at: 809 809
Size: 3857
AccI GT'mk_AC
Cuts at: 895 895
Size: 3857
Acc65I G'GTAC_C
Cuts at: 873 873
Size: 3857
AclI AA'CG_TT
Cuts at: 3697 3697
Size: 3857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1682 2114 2861 3129 1682
Size: 432 747 268 2410
Fragments arranged by size:
2410 747 432 268
AflIII A'CryG_T
Cuts at: 1209 2313 3290 1209
Size: 1104 977 1776
Fragments arranged by size:
1776 1104 977
AgeI A'CCGG_T
Cuts at: 2245 2245
Size: 3857
AlwNI CAG_nnn'CTG
Cuts at: 172 1162 2729 172
Size: 990 1567 1300
Fragments arranged by size:
1567 1300 990
ApaI G_GGCC'C
Cuts at: 322 322
Size: 3857
ApaLI G'TGCA_C
Cuts at: 2627 2627
Size: 3857
ApoI r'AATT_y
Cuts at: 861 3210 3466 861
Size: 2349 256 1252
Fragments arranged by size:
2349 1252 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3857
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
AvaI C'yCGr_G
Cuts at: 877 877
Size: 3857
BamHI G'GATC_C
Cuts at: 882 882
Size: 3857
BanI G'GyrC_C
Cuts at: 268 873 1287 1535 1570 268
Size: 605 414 248 35 2555
Fragments arranged by size:
2555 605 414 248 35
BanII G_rGCy'C
Cuts at: 305 322 871 1901 305
Size: 17 549 1030 2261
Fragments arranged by size:
2261 1030 549 17
BbeI G_GCGC'C
Cuts at: 1291 1539 1291
Size: 248 3609
BbsI GAAGACnn'nnnn_
Cuts at: 682 682
Size: 3857
BciVI GTATCCnnnnn_n'
Cuts at: 1755 2516 3857 1755
Size: 761 1341 1755
Fragments arranged by size:
1755 1341 761
BclI T'GATC_A
Cuts at: 1377 1377
Size: 3857
BglI GCCn_nnn'nGGC
Cuts at: 592 592
Size: 3857
BglII A'GATC_T
Cuts at: 1372 3409 1372
Size: 2037 1820
BlpI GC'TnA_GC
Cuts at: 969 969
Size: 3857
Bme1580I G_kGCm'C
Cuts at: 322 1482 1575 2631 322
Size: 1160 93 1056 1548
Fragments arranged by size:
1548 1160 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1069 1150 1485 3629 1069
Size: 81 335 2144 1297
Fragments arranged by size:
2144 1297 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 3857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 326 212
Size: 114 3743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1912 2404 2702 1033
Size: 879 492 298 2188
Fragments arranged by size:
2188 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 386 3561 386
Size: 3175 682
BsaAI yAC'GTr
Cuts at: 469 1210 469
Size: 741 3116
BsaBI GATnn'nnATC
Cuts at: 6 1371 3408 6
Size: 1365 2037 455
Fragments arranged by size:
2037 1365 455
BsaHI Gr'CG_yC
Cuts at: 806 1288 1536 2238 3734 806
Size: 482 248 702 1496 929
Fragments arranged by size:
1496 929 702 482 248
BsaWI w'CCGG_w
Cuts at: 408 1046 1567 2245 2519 2666 3077 408
Size: 638 521 678 274 147 411 1188
Fragments arranged by size:
1188 678 638 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 116 146 116
Size: 30 3827
BseYI C'CCAG_C
Cuts at: 1282 2040 2617 3113 1282
Size: 758 577 496 2026
Fragments arranged by size:
2026 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1399 1445 2653 104
Size: 1295 46 1208 1308
Fragments arranged by size:
1308 1295 1208 46
BsiHKAI G_wGCw'C
Cuts at: 871 1649 1839 2631 871
Size: 778 190 792 2097
Fragments arranged by size:
2097 792 778 190
BsmI GAATG_Cn'
Cuts at: 612 612
Size: 3857
Bsp1286I G_dGCh'C
Cuts at: 305 322 871 1482 1575 1649 1839 1901 2631
Size: 17 549 611 93 74 190 62 730
Cuts at: 2631 305
Size: 1531
Fragments arranged by size:
1531 730 611 549 190 93 74 62
17
BspEI T'CCGG_A
Cuts at: 408 3077 408
Size: 2669 1188
BspHI T'CATG_A
Cuts at: 3033 3033
Size: 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 208 907 1121 1423 1804 208
Size: 699 214 302 381 2261
Fragments arranged by size:
2261 699 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2149 2203 3687 3852 50
Size: 2099 54 1484 165 55
Fragments arranged by size:
2099 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1769 1769
Size: 3857
BsrFI r'CCGG_y
Cuts at: 1855 2036 2245 1855
Size: 181 209 3467
Fragments arranged by size:
3467 209 181
BssSI C'ACGA_G
Cuts at: 2128 2486 2128
Size: 358 3499
BstAPI GCAn_nnn'nTGC
Cuts at: 214 1234 214
Size: 1020 2837
BstBI TT'CG_AA
Cuts at: 2218 2218
Size: 3857
BstEII G'GTnAC_C
Cuts at: 365 365
Size: 3857
BstYI r'GATC_y
Cuts at: 1 696 882 1349 1372 1707 1953 2965 3051
Size: 695 186 467 23 335 246 1012 86
Cuts at: 3051 3063 3409 1
Size: 12 346 449
Fragments arranged by size:
1012 695 467 449 346 335 246 186
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1807 1988 2151 1807
Size: 181 163 3513
Fragments arranged by size:
3513 181 163
BtsI GCAGTG_nn'
Cuts at: 215 1100 215
Size: 885 2972
DraI TTT'AAA
Cuts at: 856 856
Size: 3857
DrdI GACnn_nn'nnGTC
Cuts at: 1563 2421 1563
Size: 858 2999
EaeI y'GGCC_r
Cuts at: 593 792 830 1442 1616 2007 2034 3379 593
Size: 199 38 612 174 391 27 1345 1071
Fragments arranged by size:
1345 1071 612 391 199 174 38 27
EagI C'GGCC_G
Cuts at: 1442 1442
Size: 3857
EarI CTCTTCn'nnn_
Cuts at: 1880 2090 1880
Size: 210 3647
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2065 2375 2521 3667 2065
Size: 310 146 1146 2255
Fragments arranged by size:
2255 1146 310 146
EcoICRI GAG'CTC
Cuts at: 869 869
Size: 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 326 1682 2114 2861 3129 212
Size: 114 1356 432 747 268 940
Fragments arranged by size:
1356 940 747 432 268 114
EcoO109I rG'GnC_Cy
Cuts at: 318 319 337 355 996 318
Size: 1 18 18 641 3179
Fragments arranged by size:
3179 641 18 18 1
EcoRI G'AATT_C
Cuts at: 861 861
Size: 3857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1876 1876
Size: 3857
FspI TGC'GCA
Cuts at: 1638 1638
Size: 3857
HaeII r_GCGC'y
Cuts at: 1291 1539 2561 3540 1291
Size: 248 1022 979 1608
Fragments arranged by size:
1608 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 477 509 1699 1729 1731 1761 477
Size: 32 1190 30 2 30 2573
Fragments arranged by size:
2573 1190 32 30 30 2
HincII GTy'rAC
Cuts at: 896 896
Size: 3857
KasI G'GCGC_C
Cuts at: 1287 1535 1287
Size: 248 3609
KpnI G_GTAC'C
Cuts at: 877 877
Size: 3857
MluI A'CGCG_T
Cuts at: 3290 3290
Size: 3857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2528 2712 2528
Size: 184 3673
MscI TGG'CCA
Cuts at: 595 794 1618 595
Size: 199 824 2834
Fragments arranged by size:
2834 824 199
MslI CAynn'nnrTG
Cuts at: 1846 1973 1846
Size: 127 3730
MspA1I CmG'CkG
Cuts at: 968 1282 1642 2655 2900 968
Size: 314 360 1013 245 1925
Fragments arranged by size:
1925 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 3857
NarI GG'CG_CC
Cuts at: 1288 1536 1288
Size: 248 3609
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 3857
NspI r_CATG'y
Cuts at: 910 1410 2317 910
Size: 500 907 2450
Fragments arranged by size:
2450 907 500
PciI A'CATG_T
Cuts at: 2313 2313
Size: 3857
PfoI T'CCnGG_A
Cuts at: 342 342
Size: 3857
PmeI GTTT'AAAC
Cuts at: 856 856
Size: 3857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3825
PspOMI G'GGCC_C
Cuts at: 318 318
Size: 3857
PstI C_TGCA'G
Cuts at: 210 615 904 1589 210
Size: 405 289 685 2478
Fragments arranged by size:
2478 685 405 289
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3857
PvuII CAG'CTG
Cuts at: 1282 1642 1282
Size: 360 3497
RsrII CG'GwC_CG
Cuts at: 2052 2052
Size: 3857
SacI G_AGCT'C
Cuts at: 871 871
Size: 3857
SalI G'TCGA_C
Cuts at: 894 894
Size: 3857
SapI GCTCTTCn'nnn_
Cuts at: 1880 2090 1880
Size: 210 3647
SbfI CC_TGCA'GG
Cuts at: 904 904
Size: 3857
ScaI AGT'ACT
Cuts at: 3374 3374
Size: 3857
SfcI C'TryA_G
Cuts at: 33 206 219 611 900 1585 2578 2769 33
Size: 173 13 392 289 685 993 191 1121
Fragments arranged by size:
1121 993 685 392 289 191 173 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 592 592
Size: 3857
SfoI GGC'GCC
Cuts at: 1289 1537 1289
Size: 248 3609
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
SmaI CCC'GGG
Cuts at: 879 879
Size: 3857
SmlI C'TyrA_G
Cuts at: 1012 1927 2419 2681 1012
Size: 915 492 262 2188
Fragments arranged by size:
2188 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3857
SphI G_CATG'C
Cuts at: 910 1410 910
Size: 500 3357
StuI AGG'CCT
Cuts at: 565 586 565
Size: 21 3836
StyI C'CwwG_G
Cuts at: 272 297 991 1345 272
Size: 25 694 354 2784
Fragments arranged by size:
2784 694 354 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1440 1573 2032 2241 1440
Size: 133 459 209 3056
Fragments arranged by size:
3056 459 209 133
TatI w'GTAC_w
Cuts at: 3372 3372
Size: 3857
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 116 148 723 755 3374 3406 116
Size: 32 575 32 2619 32 567
Fragments arranged by size:
2619 575 567 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1654 1654
Size: 3857
XbaI T'CTAG_A
Cuts at: 888 888
Size: 3857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 825 1352 825
Size: 527 3330
XmaI C'CCGG_G
Cuts at: 877 877
Size: 3857
XmnI GAAnn'nnTTC
Cuts at: 517 517
Size: 3857
ZraI GAC'GTC
Cuts at: 807 807
Size: 3857
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI