Restriction map for
pF1KB6989
(Circular) (Six-base) MAPSORT of: pF1KB6989.seq Check: 5893 from: 1 to: 3770
pF1KB6989 3770 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 808 808
Size: 3770
Acc65I G'GTAC_C
Cuts at: 786 786
Size: 3770
AclI AA'CG_TT
Cuts at: 3610 3610
Size: 3770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1595 2027 2774 3042 1595
Size: 432 747 268 2323
Fragments arranged by size:
2323 747 432 268
AflIII A'CryG_T
Cuts at: 1122 2226 3203 1122
Size: 1104 977 1689
Fragments arranged by size:
1689 1104 977
AgeI A'CCGG_T
Cuts at: 2158 2158
Size: 3770
AhdI GACnn_n'nnGTC
Cuts at: 267 267
Size: 3770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 760 760
Size: 3770
AlwNI CAG_nnn'CTG
Cuts at: 316 1075 2642 316
Size: 759 1567 1444
Fragments arranged by size:
1567 1444 759
ApaLI G'TGCA_C
Cuts at: 296 2540 296
Size: 2244 1526
ApoI r'AATT_y
Cuts at: 751 774 3123 3379 751
Size: 23 2349 256 1142
Fragments arranged by size:
2349 1142 256 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 3770
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
AvaI C'yCGr_G
Cuts at: 143 221 610 790 143
Size: 78 389 180 3123
Fragments arranged by size:
3123 389 180 78
BamHI G'GATC_C
Cuts at: 795 795
Size: 3770
BanI G'GyrC_C
Cuts at: 786 1200 1448 1483 786
Size: 414 248 35 3073
Fragments arranged by size:
3073 414 248 35
BanII G_rGCy'C
Cuts at: 362 784 1814 362
Size: 422 1030 2318
Fragments arranged by size:
2318 1030 422
BbeI G_GCGC'C
Cuts at: 1204 1452 1204
Size: 248 3522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3736
BciVI GTATCCnnnnn_n'
Cuts at: 1668 2429 3770 1668
Size: 761 1341 1668
Fragments arranged by size:
1668 1341 761
BclI T'GATC_A
Cuts at: 1290 1290
Size: 3770
BglI GCCn_nnn'nGGC
Cuts at: 340 665 340
Size: 325 3445
BglII A'GATC_T
Cuts at: 1285 3322 1285
Size: 2037 1733
BlpI GC'TnA_GC
Cuts at: 882 882
Size: 3770
Bme1580I G_kGCm'C
Cuts at: 300 1395 1488 2544 300
Size: 1095 93 1056 1526
Fragments arranged by size:
1526 1095 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 982 1063 1398 3542 982
Size: 81 335 2144 1210
Fragments arranged by size:
2144 1210 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 3770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 397 376
Size: 21 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 654 946 1825 2317 2615 654
Size: 292 879 492 298 1809
Fragments arranged by size:
1809 879 492 298 292
BsaI GGTCTCn'nnnn_
Cuts at: 3474 3474
Size: 3770
BsaAI yAC'GTr
Cuts at: 1123 1123
Size: 3770
BsaBI GATnn'nnATC
Cuts at: 6 1284 3321 6
Size: 1278 2037 455
Fragments arranged by size:
2037 1278 455
BsaHI Gr'CG_yC
Cuts at: 1201 1449 2151 3647 1201
Size: 248 702 1496 1324
Fragments arranged by size:
1496 1324 702 248
BsaWI w'CCGG_w
Cuts at: 959 1480 2158 2432 2579 2990 959
Size: 521 678 274 147 411 1739
Fragments arranged by size:
1739 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 482 512 482
Size: 30 3740
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 628 628
Size: 3770
BseYI C'CCAG_C
Cuts at: 473 1195 1953 2530 3026 473
Size: 722 758 577 496 1217
Fragments arranged by size:
1217 758 722 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 437 639 262
Size: 175 202 3393
Fragments arranged by size:
3393 202 175
BsiEI CG_ry'CG
Cuts at: 104 1312 1358 2566 104
Size: 1208 46 1208 1308
Fragments arranged by size:
1308 1208 1208 46
BsiHKAI G_wGCw'C
Cuts at: 126 300 362 784 1562 1752 2544 126
Size: 174 62 422 778 190 792 1352
Fragments arranged by size:
1352 792 778 422 190 174 62
BsmI GAATG_Cn'
Cuts at: 524 524
Size: 3770
Bsp1286I G_dGCh'C
Cuts at: 126 300 362 784 1395 1488 1562 1752 1814
Size: 174 62 422 611 93 74 190 62
Cuts at: 1814 2544 126
Size: 730 1352
Fragments arranged by size:
1352 730 611 422 190 174 93 74
62 62
BspEI T'CCGG_A
Cuts at: 2990 2990
Size: 3770
BspHI T'CATG_A
Cuts at: 2946 2946
Size: 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 820 1034 1336 1717 820
Size: 214 302 381 2873
Fragments arranged by size:
2873 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2062 2116 3600 3765 50
Size: 2012 54 1484 165 55
Fragments arranged by size:
2012 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 744 1682 744
Size: 938 2832
BsrFI r'CCGG_y
Cuts at: 709 1768 1949 2158 709
Size: 1059 181 209 2321
Fragments arranged by size:
2321 1059 209 181
BssSI C'ACGA_G
Cuts at: 280 2041 2399 280
Size: 1761 358 1651
Fragments arranged by size:
1761 1651 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1147 1147
Size: 3770
BstBI TT'CG_AA
Cuts at: 2131 2131
Size: 3770
BstXI CCAn_nnnn'nTGG
Cuts at: 177 177
Size: 3770
BstYI r'GATC_y
Cuts at: 1 795 1262 1285 1620 1866 2878 2964 2976
Size: 794 467 23 335 246 1012 86 12
Cuts at: 2976 3322 1
Size: 346 449
Fragments arranged by size:
1012 794 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 329 363 329
Size: 34 3736
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1720 1901 2064 1720
Size: 181 163 3426
Fragments arranged by size:
3426 181 163
BtsI GCAGTG_nn'
Cuts at: 188 1013 188
Size: 825 2945
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 377 412 377
Size: 35 3735
DraI TTT'AAA
Cuts at: 769 769
Size: 3770
DrdI GACnn_nn'nnGTC
Cuts at: 724 1476 2334 724
Size: 752 858 2160
Fragments arranged by size:
2160 858 752
EaeI y'GGCC_r
Cuts at: 332 341 650 1355 1529 1920 1947 3292 332
Size: 9 309 705 174 391 27 1345 810
Fragments arranged by size:
1345 810 705 391 309 174 27 9
EagI C'GGCC_G
Cuts at: 1355 1355
Size: 3770
EarI CTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1978 2288 2434 3580 1978
Size: 310 146 1146 2168
Fragments arranged by size:
2168 1146 310 146
EcoICRI GAG'CTC
Cuts at: 360 782 360
Size: 422 3348
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 397 1595 2027 2774 3042 376
Size: 21 1198 432 747 268 1104
Fragments arranged by size:
1198 1104 747 432 268 21
EcoO109I rG'GnC_Cy
Cuts at: 909 909
Size: 3770
EcoRI G'AATT_C
Cuts at: 774 774
Size: 3770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1789 1789
Size: 3770
FspI TGC'GCA
Cuts at: 1551 1551
Size: 3770
HaeII r_GCGC'y
Cuts at: 627 1204 1452 2474 3453 627
Size: 577 248 1022 979 944
Fragments arranged by size:
1022 979 944 577 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 253 285 433 465 1612 1642 1644 1674 253
Size: 32 148 32 1147 30 2 30 2349
Fragments arranged by size:
2349 1147 148 32 32 30 30 2
HincII GTy'rAC
Cuts at: 809 809
Size: 3770
KasI G'GCGC_C
Cuts at: 1200 1448 1200
Size: 248 3522
KpnI G_GTAC'C
Cuts at: 790 790
Size: 3770
MluI A'CGCG_T
Cuts at: 3203 3203
Size: 3770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2441 2625 2441
Size: 184 3586
MscI TGG'CCA
Cuts at: 652 1531 652
Size: 879 2891
MslI CAynn'nnrTG
Cuts at: 175 181 1759 1886 175
Size: 6 1578 127 2059
Fragments arranged by size:
2059 1578 127 6
MspA1I CmG'CkG
Cuts at: 276 313 354 706 881 1195 1555 2568 2813
Size: 37 41 352 175 314 360 1013 245
Cuts at: 2813 276
Size: 1233
Fragments arranged by size:
1233 1013 360 352 314 245 175 41
37
NaeI GCC'GGC
Cuts at: 1951 1951
Size: 3770
NarI GG'CG_CC
Cuts at: 1201 1449 1201
Size: 248 3522
NcoI C'CATG_G
Cuts at: 363 363
Size: 3770
NgoMIV G'CCGG_C
Cuts at: 1949 1949
Size: 3770
NspI r_CATG'y
Cuts at: 606 823 1323 2230 606
Size: 217 500 907 2146
Fragments arranged by size:
2146 907 500 217
PasI CC'CwG_GG
Cuts at: 510 510
Size: 3770
PciI A'CATG_T
Cuts at: 2226 2226
Size: 3770
PflMI CCAn_nnn'nTGG
Cuts at: 330 330
Size: 3770
PmeI GTTT'AAAC
Cuts at: 769 769
Size: 3770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 3738
PstI C_TGCA'G
Cuts at: 234 817 1502 234
Size: 583 685 2502
Fragments arranged by size:
2502 685 583
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3770
PvuII CAG'CTG
Cuts at: 313 354 706 1195 1555 313
Size: 41 352 489 360 2528
Fragments arranged by size:
2528 489 360 352 41
RsrII CG'GwC_CG
Cuts at: 1965 1965
Size: 3770
SacI G_AGCT'C
Cuts at: 362 784 362
Size: 422 3348
SalI G'TCGA_C
Cuts at: 807 807
Size: 3770
SapI GCTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
SbfI CC_TGCA'GG
Cuts at: 817 817
Size: 3770
ScaI AGT'ACT
Cuts at: 3287 3287
Size: 3770
SexAI A'CCwGG_T
Cuts at: 689 689
Size: 3770
SfcI C'TryA_G
Cuts at: 33 230 813 1498 2491 2682 33
Size: 197 583 685 993 191 1121
Fragments arranged by size:
1121 993 685 583 197 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 340 340
Size: 3770
SfoI GGC'GCC
Cuts at: 1202 1450 1202
Size: 248 3522
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
SmaI CCC'GGG
Cuts at: 145 792 145
Size: 647 3123
SmlI C'TyrA_G
Cuts at: 669 925 1840 2332 2594 669
Size: 256 915 492 262 1845
Fragments arranged by size:
1845 915 492 262 256
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3770
SphI G_CATG'C
Cuts at: 823 1323 823
Size: 500 3270
StyI C'CwwG_G
Cuts at: 363 904 1258 363
Size: 541 354 2875
Fragments arranged by size:
2875 541 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1353 1486 1945 2154 1353
Size: 133 459 209 2969
Fragments arranged by size:
2969 459 209 133
TatI w'GTAC_w
Cuts at: 3285 3285
Size: 3770
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 3738
Tth111I GACn'n_nGTC
Cuts at: 1567 1567
Size: 3770
XbaI T'CTAG_A
Cuts at: 801 801
Size: 3770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 180 1265 180
Size: 1085 2685
XmaI C'CCGG_G
Cuts at: 143 790 143
Size: 647 3123
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI