Restriction map for
pF1KB6992
(Circular) (Six-base) MAPSORT of: pF1KB6992.seq Check: 9288 from: 1 to: 3869
pF1KB6992 3869 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 907 907
Size: 3869
Acc65I G'GTAC_C
Cuts at: 885 885
Size: 3869
AclI AA'CG_TT
Cuts at: 3709 3709
Size: 3869
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1694 2126 2873 3141 1694
Size: 432 747 268 2422
Fragments arranged by size:
2422 747 432 268
AfeI AGC'GCT
Cuts at: 285 590 285
Size: 305 3564
AflIII A'CryG_T
Cuts at: 1221 2325 3302 1221
Size: 1104 977 1788
Fragments arranged by size:
1788 1104 977
AgeI A'CCGG_T
Cuts at: 2257 2257
Size: 3869
AlwNI CAG_nnn'CTG
Cuts at: 1174 2741 1174
Size: 1567 2302
ApaI G_GGCC'C
Cuts at: 420 420
Size: 3869
ApaLI G'TGCA_C
Cuts at: 2639 2639
Size: 3869
ApoI r'AATT_y
Cuts at: 873 3222 3478 873
Size: 2349 256 1264
Fragments arranged by size:
2349 1264 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3869
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
AvaI C'yCGr_G
Cuts at: 488 889 488
Size: 401 3468
BamHI G'GATC_C
Cuts at: 894 894
Size: 3869
BanI G'GyrC_C
Cuts at: 375 885 1299 1547 1582 375
Size: 510 414 248 35 2662
Fragments arranged by size:
2662 510 414 248 35
BanII G_rGCy'C
Cuts at: 420 496 883 1913 420
Size: 76 387 1030 2376
Fragments arranged by size:
2376 1030 387 76
BbeI G_GCGC'C
Cuts at: 379 1303 1551 379
Size: 924 248 2697
Fragments arranged by size:
2697 924 248
BciVI GTATCCnnnnn_n'
Cuts at: 1767 2528 3869 1767
Size: 761 1341 1767
Fragments arranged by size:
1767 1341 761
BclI T'GATC_A
Cuts at: 1389 1389
Size: 3869
BglII A'GATC_T
Cuts at: 1384 3421 1384
Size: 2037 1832
BlpI GC'TnA_GC
Cuts at: 168 981 168
Size: 813 3056
Bme1580I G_kGCm'C
Cuts at: 420 1494 1587 2643 420
Size: 1074 93 1056 1646
Fragments arranged by size:
1646 1074 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 401 1081 1162 1497 3641 401
Size: 680 81 335 2144 629
Fragments arranged by size:
2144 680 629 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 3869
Bpu10I CC'TnA_GC
Cuts at: 295 295
Size: 3869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1924 2416 2714 1045
Size: 879 492 298 2200
Fragments arranged by size:
2200 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3573 3573
Size: 3869
BsaAI yAC'GTr
Cuts at: 1222 1222
Size: 3869
BsaBI GATnn'nnATC
Cuts at: 6 1383 3420 6
Size: 1377 2037 455
Fragments arranged by size:
2037 1377 455
BsaHI Gr'CG_yC
Cuts at: 376 1300 1548 2250 3746 376
Size: 924 248 702 1496 499
Fragments arranged by size:
1496 924 702 499 248
BsaWI w'CCGG_w
Cuts at: 1058 1579 2257 2531 2678 3089 1058
Size: 521 678 274 147 411 1838
Fragments arranged by size:
1838 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 653 683 653
Size: 30 3839
BseYI C'CCAG_C
Cuts at: 1294 2052 2629 3125 1294
Size: 758 577 496 2038
Fragments arranged by size:
2038 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 246 274 1411 1457 2665 104
Size: 142 28 1137 46 1208 1308
Fragments arranged by size:
1308 1208 1137 142 46 28
BsiHKAI G_wGCw'C
Cuts at: 473 883 1661 1851 2643 473
Size: 410 778 190 792 1699
Fragments arranged by size:
1699 792 778 410 190
BsmBI CGTCTCn'nnnn_
Cuts at: 161 161
Size: 3869
Bsp1286I G_dGCh'C
Cuts at: 420 473 496 883 1494 1587 1661 1851 1913
Size: 53 23 387 611 93 74 190 62
Cuts at: 1913 2643 420
Size: 730 1646
Fragments arranged by size:
1646 730 611 387 190 93 74 62
53 23
BspEI T'CCGG_A
Cuts at: 3089 3089
Size: 3869
BspHI T'CATG_A
Cuts at: 3045 3045
Size: 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 919 1133 1435 1816 919
Size: 214 302 381 2972
Fragments arranged by size:
2972 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2161 2215 3699 3864 50
Size: 2111 54 1484 165 55
Fragments arranged by size:
2111 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1781 1781
Size: 3869
BsrFI r'CCGG_y
Cuts at: 1867 2048 2257 1867
Size: 181 209 3479
Fragments arranged by size:
3479 209 181
BsrGI T'GTAC_A
Cuts at: 521 521
Size: 3869
BssHII G'CGCG_C
Cuts at: 499 501 499
Size: 2 3867
BssSI C'ACGA_G
Cuts at: 2140 2498 2140
Size: 358 3511
BstAPI GCAn_nnn'nTGC
Cuts at: 1246 1246
Size: 3869
BstBI TT'CG_AA
Cuts at: 2230 2230
Size: 3869
BstYI r'GATC_y
Cuts at: 1 894 1361 1384 1719 1965 2977 3063 3075
Size: 893 467 23 335 246 1012 86 12
Cuts at: 3075 3421 1
Size: 346 449
Fragments arranged by size:
1012 893 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 356 356
Size: 3869
BtgI C'CryG_G
Cuts at: 212 309 476 506 622 212
Size: 97 167 30 116 3459
Fragments arranged by size:
3459 167 116 97 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 187 1819 2000 2163 187
Size: 1632 181 163 1893
Fragments arranged by size:
1893 1632 181 163
BtsI GCAGTG_nn'
Cuts at: 1112 1112
Size: 3869
DraI TTT'AAA
Cuts at: 868 868
Size: 3869
DrdI GACnn_nn'nnGTC
Cuts at: 1575 2433 1575
Size: 858 3011
EaeI y'GGCC_r
Cuts at: 243 271 288 1454 1628 2019 2046 3391 243
Size: 28 17 1166 174 391 27 1345 721
Fragments arranged by size:
1345 1166 721 391 174 28 27 17
EagI C'GGCC_G
Cuts at: 243 271 1454 243
Size: 28 1183 2658
Fragments arranged by size:
2658 1183 28
EarI CTCTTCn'nnn_
Cuts at: 606 1892 2102 606
Size: 1286 210 2373
Fragments arranged by size:
2373 1286 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2077 2387 2533 3679 2077
Size: 310 146 1146 2267
Fragments arranged by size:
2267 1146 310 146
EcoICRI GAG'CTC
Cuts at: 881 881
Size: 3869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1694 2126 2873 3141 1694
Size: 432 747 268 2422
Fragments arranged by size:
2422 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1008 1008
Size: 3869
EcoRI G'AATT_C
Cuts at: 873 873
Size: 3869
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1888 1888
Size: 3869
FspI TGC'GCA
Cuts at: 1650 1650
Size: 3869
HaeII r_GCGC'y
Cuts at: 287 379 592 1303 1551 2573 3552 287
Size: 92 213 711 248 1022 979 604
Fragments arranged by size:
1022 979 711 604 248 213 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 653 685 771 803 1711 1741 1743 1773 653
Size: 32 86 32 908 30 2 30 2749
Fragments arranged by size:
2749 908 86 32 32 30 30 2
HincII GTy'rAC
Cuts at: 908 908
Size: 3869
KasI G'GCGC_C
Cuts at: 375 1299 1547 375
Size: 924 248 2697
Fragments arranged by size:
2697 924 248
KpnI G_GTAC'C
Cuts at: 889 889
Size: 3869
MluI A'CGCG_T
Cuts at: 3302 3302
Size: 3869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2540 2724 2540
Size: 184 3685
MscI TGG'CCA
Cuts at: 1630 1630
Size: 3869
MslI CAynn'nnrTG
Cuts at: 1858 1985 1858
Size: 127 3742
MspA1I CmG'CkG
Cuts at: 214 276 311 334 508 980 1294 1654 2667
Size: 62 35 23 174 472 314 360 1013
Cuts at: 2667 2912 214
Size: 245 1171
Fragments arranged by size:
1171 1013 472 360 314 245 174 62
35 23
NaeI GCC'GGC
Cuts at: 2050 2050
Size: 3869
NarI GG'CG_CC
Cuts at: 376 1300 1548 376
Size: 924 248 2697
Fragments arranged by size:
2697 924 248
NgoMIV G'CCGG_C
Cuts at: 2048 2048
Size: 3869
NspI r_CATG'y
Cuts at: 444 922 1422 2329 444
Size: 478 500 907 1984
Fragments arranged by size:
1984 907 500 478
PciI A'CATG_T
Cuts at: 2325 2325
Size: 3869
PmeI GTTT'AAAC
Cuts at: 868 868
Size: 3869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 3837
PspOMI G'GGCC_C
Cuts at: 416 416
Size: 3869
PstI C_TGCA'G
Cuts at: 916 1601 916
Size: 685 3184
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3869
PvuII CAG'CTG
Cuts at: 1294 1654 1294
Size: 360 3509
RsrII CG'GwC_CG
Cuts at: 2064 2064
Size: 3869
SacI G_AGCT'C
Cuts at: 883 883
Size: 3869
SacII CC_GC'GG
Cuts at: 215 312 509 215
Size: 97 197 3575
Fragments arranged by size:
3575 197 97
SalI G'TCGA_C
Cuts at: 906 906
Size: 3869
SapI GCTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
SbfI CC_TGCA'GG
Cuts at: 916 916
Size: 3869
ScaI AGT'ACT
Cuts at: 3386 3386
Size: 3869
SfcI C'TryA_G
Cuts at: 33 912 1597 2590 2781 33
Size: 879 685 993 191 1121
Fragments arranged by size:
1121 993 879 685 191
SfoI GGC'GCC
Cuts at: 377 1301 1549 377
Size: 924 248 2697
Fragments arranged by size:
2697 924 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
SmaI CCC'GGG
Cuts at: 490 891 490
Size: 401 3468
SmlI C'TyrA_G
Cuts at: 1024 1939 2431 2693 1024
Size: 915 492 262 2200
Fragments arranged by size:
2200 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3869
SphI G_CATG'C
Cuts at: 444 922 1422 444
Size: 478 500 2891
Fragments arranged by size:
2891 500 478
StyI C'CwwG_G
Cuts at: 300 812 1003 1357 300
Size: 512 191 354 2812
Fragments arranged by size:
2812 512 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 420 590 1452 1585 2044 2253 420
Size: 170 862 133 459 209 2036
Fragments arranged by size:
2036 862 459 209 170 133
TatI w'GTAC_w
Cuts at: 319 521 705 3384 319
Size: 202 184 2679 804
Fragments arranged by size:
2679 804 202 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 3837
Tth111I GACn'n_nGTC
Cuts at: 1666 1666
Size: 3869
XbaI T'CTAG_A
Cuts at: 900 900
Size: 3869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1364 1364
Size: 3869
XmaI C'CCGG_G
Cuts at: 488 889 488
Size: 401 3468
XmnI GAAnn'nnTTC
Cuts at: 803 803
Size: 3869
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BseRI BsgI BsiWI BsmI BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI