Restriction map for
pF1KB6994
(Circular) (Six-base) MAPSORT of: pF1KB6994.seq Check: 3817 from: 1 to: 3872
pF1KB6994 3872 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 156 156
Size: 3872
AccI GT'mk_AC
Cuts at: 910 910
Size: 3872
Acc65I G'GTAC_C
Cuts at: 888 888
Size: 3872
AclI AA'CG_TT
Cuts at: 3712 3712
Size: 3872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 1697 2129 2876 3144 428
Size: 1269 432 747 268 1156
Fragments arranged by size:
1269 1156 747 432 268
AflIII A'CryG_T
Cuts at: 1224 2328 3305 1224
Size: 1104 977 1791
Fragments arranged by size:
1791 1104 977
AgeI A'CCGG_T
Cuts at: 2260 2260
Size: 3872
AlwNI CAG_nnn'CTG
Cuts at: 717 1177 2744 717
Size: 460 1567 1845
Fragments arranged by size:
1845 1567 460
ApaLI G'TGCA_C
Cuts at: 2642 2642
Size: 3872
ApoI r'AATT_y
Cuts at: 388 501 720 818 876 3225 3481 388
Size: 113 219 98 58 2349 256 779
Fragments arranged by size:
2349 779 256 219 113 98 58
AseI AT'TA_AT
Cuts at: 20 20
Size: 3872
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
AvaI C'yCGr_G
Cuts at: 892 892
Size: 3872
BamHI G'GATC_C
Cuts at: 651 897 651
Size: 246 3626
BanI G'GyrC_C
Cuts at: 888 1302 1550 1585 888
Size: 414 248 35 3175
Fragments arranged by size:
3175 414 248 35
BanII G_rGCy'C
Cuts at: 886 1916 886
Size: 1030 2842
BbeI G_GCGC'C
Cuts at: 1306 1554 1306
Size: 248 3624
BbsI GAAGACnn'nnnn_
Cuts at: 602 602
Size: 3872
BbvCI CC'TCA_GC
Cuts at: 343 343
Size: 3872
BciVI GTATCCnnnnn_n'
Cuts at: 1770 2531 3872 1770
Size: 761 1341 1770
Fragments arranged by size:
1770 1341 761
BclI T'GATC_A
Cuts at: 417 1392 417
Size: 975 2897
BglII A'GATC_T
Cuts at: 665 1387 3424 665
Size: 722 2037 1113
Fragments arranged by size:
2037 1113 722
BlpI GC'TnA_GC
Cuts at: 205 984 205
Size: 779 3093
Bme1580I G_kGCm'C
Cuts at: 1497 1590 2646 1497
Size: 93 1056 2723
Fragments arranged by size:
2723 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1084 1165 1500 3644 1084
Size: 81 335 2144 1312
Fragments arranged by size:
2144 1312 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2239 2239
Size: 3872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 349 531 281
Size: 68 182 3622
Fragments arranged by size:
3622 182 68
Bpu10I CC'TnA_GC
Cuts at: 343 343
Size: 3872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1927 2419 2717 1048
Size: 879 492 298 2203
Fragments arranged by size:
2203 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 505 3576 505
Size: 3071 801
BsaAI yAC'GTr
Cuts at: 1225 1225
Size: 3872
BsaBI GATnn'nnATC
Cuts at: 6 1386 3423 6
Size: 1380 2037 455
Fragments arranged by size:
2037 1380 455
BsaHI Gr'CG_yC
Cuts at: 1303 1551 2253 3749 1303
Size: 248 702 1496 1426
Fragments arranged by size:
1496 1426 702 248
BsaWI w'CCGG_w
Cuts at: 765 1061 1582 2260 2534 2681 3092 765
Size: 296 521 678 274 147 411 1545
Fragments arranged by size:
1545 678 521 411 296 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 220
Size: 3872
BseYI C'CCAG_C
Cuts at: 131 1297 2055 2632 3128 131
Size: 1166 758 577 496 875
Fragments arranged by size:
1166 875 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1414 1460 2668 104
Size: 1310 46 1208 1308
Fragments arranged by size:
1310 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 886 1664 1854 2646 886
Size: 778 190 792 2112
Fragments arranged by size:
2112 792 778 190
BsmI GAATG_Cn'
Cuts at: 639 864 639
Size: 225 3647
Bsp1286I G_dGCh'C
Cuts at: 886 1497 1590 1664 1854 1916 2646 886
Size: 611 93 74 190 62 730 2112
Fragments arranged by size:
2112 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3092 3092
Size: 3872
BspHI T'CATG_A
Cuts at: 420 3048 420
Size: 2628 1244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 156 347 922 1136 1438 1819 156
Size: 191 575 214 302 381 2209
Fragments arranged by size:
2209 575 381 302 214 191
BsrBI CCG'CTC
Cuts at: 50 2164 2218 3702 3867 50
Size: 2114 54 1484 165 55
Fragments arranged by size:
2114 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1784 1784
Size: 3872
BsrFI r'CCGG_y
Cuts at: 200 1870 2051 2260 200
Size: 1670 181 209 1812
Fragments arranged by size:
1812 1670 209 181
BssHII G'CGCG_C
Cuts at: 220 220
Size: 3872
BssSI C'ACGA_G
Cuts at: 2143 2501 2143
Size: 358 3514
BstAPI GCAn_nnn'nTGC
Cuts at: 1249 1249
Size: 3872
BstBI TT'CG_AA
Cuts at: 2233 2233
Size: 3872
BstXI CCAn_nnnn'nTGG
Cuts at: 453 453
Size: 3872
BstYI r'GATC_y
Cuts at: 1 651 665 897 1364 1387 1722 1968 2980
Size: 650 14 232 467 23 335 246 1012
Cuts at: 2980 3066 3078 3424 1
Size: 86 12 346 449
Fragments arranged by size:
1012 650 467 449 346 335 246 232
86 23 14 12
BtgI C'CryG_G
Cuts at: 152 287 152
Size: 135 3737
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1822 2003 2166 1822
Size: 181 163 3528
Fragments arranged by size:
3528 181 163
BtsI GCAGTG_nn'
Cuts at: 202 833 1115 202
Size: 631 282 2959
Fragments arranged by size:
2959 631 282
DraI TTT'AAA
Cuts at: 871 871
Size: 3872
DrdI GACnn_nn'nnGTC
Cuts at: 1578 2436 1578
Size: 858 3014
EaeI y'GGCC_r
Cuts at: 155 272 290 1457 1631 2022 2049 3394 155
Size: 117 18 1167 174 391 27 1345 633
Fragments arranged by size:
1345 1167 633 391 174 117 27 18
EagI C'GGCC_G
Cuts at: 1457 1457
Size: 3872
EarI CTCTTCn'nnn_
Cuts at: 404 1895 2105 404
Size: 1491 210 2171
Fragments arranged by size:
2171 1491 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2080 2390 2536 3682 2080
Size: 310 146 1146 2270
Fragments arranged by size:
2270 1146 310 146
EcoICRI GAG'CTC
Cuts at: 884 884
Size: 3872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 349 428 531 1697 2129 2876 3144 281
Size: 68 79 103 1166 432 747 268 1009
Fragments arranged by size:
1166 1009 747 432 268 103 79 68
EcoO109I rG'GnC_Cy
Cuts at: 714 1011 714
Size: 297 3575
EcoRI G'AATT_C
Cuts at: 818 876 818
Size: 58 3814
EcoRV GAT'ATC
Cuts at: 429 429
Size: 3872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1891 1891
Size: 3872
FspI TGC'GCA
Cuts at: 1653 1653
Size: 3872
HaeII r_GCGC'y
Cuts at: 1306 1554 2576 3555 1306
Size: 248 1022 979 1623
Fragments arranged by size:
1623 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 837 869 1714 1744 1746 1776 837
Size: 32 845 30 2 30 2933
Fragments arranged by size:
2933 845 32 30 30 2
HincII GTy'rAC
Cuts at: 911 911
Size: 3872
HindIII A'AGCT_T
Cuts at: 640 640
Size: 3872
KasI G'GCGC_C
Cuts at: 1302 1550 1302
Size: 248 3624
KpnI G_GTAC'C
Cuts at: 892 892
Size: 3872
MluI A'CGCG_T
Cuts at: 3305 3305
Size: 3872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2727 2543
Size: 184 3688
MscI TGG'CCA
Cuts at: 274 292 1633 274
Size: 18 1341 2513
Fragments arranged by size:
2513 1341 18
MslI CAynn'nnrTG
Cuts at: 1861 1988 1861
Size: 127 3745
MspA1I CmG'CkG
Cuts at: 154 175 983 1297 1657 2670 2915 154
Size: 21 808 314 360 1013 245 1111
Fragments arranged by size:
1111 1013 808 360 314 245 21
NaeI GCC'GGC
Cuts at: 202 2053 202
Size: 1851 2021
NarI GG'CG_CC
Cuts at: 1303 1551 1303
Size: 248 3624
NgoMIV G'CCGG_C
Cuts at: 200 2051 200
Size: 1851 2021
NspI r_CATG'y
Cuts at: 213 363 687 925 1425 2332 213
Size: 150 324 238 500 907 1753
Fragments arranged by size:
1753 907 500 324 238 150
PasI CC'CwG_GG
Cuts at: 118 118
Size: 3872
PciI A'CATG_T
Cuts at: 2328 2328
Size: 3872
PfoI T'CCnGG_A
Cuts at: 254 254
Size: 3872
PmeI GTTT'AAAC
Cuts at: 871 871
Size: 3872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3840
PsiI TTA'TAA
Cuts at: 776 776
Size: 3872
PstI C_TGCA'G
Cuts at: 197 919 1604 197
Size: 722 685 2465
Fragments arranged by size:
2465 722 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3872
PvuII CAG'CTG
Cuts at: 1297 1657 1297
Size: 360 3512
RsrII CG'GwC_CG
Cuts at: 2067 2067
Size: 3872
SacI G_AGCT'C
Cuts at: 886 886
Size: 3872
SacII CC_GC'GG
Cuts at: 155 155
Size: 3872
SalI G'TCGA_C
Cuts at: 909 909
Size: 3872
SapI GCTCTTCn'nnn_
Cuts at: 1895 2105 1895
Size: 210 3662
SbfI CC_TGCA'GG
Cuts at: 919 919
Size: 3872
ScaI AGT'ACT
Cuts at: 3389 3389
Size: 3872
SexAI A'CCwGG_T
Cuts at: 611 611
Size: 3872
SfcI C'TryA_G
Cuts at: 33 193 915 1600 2593 2784 33
Size: 160 722 685 993 191 1121
Fragments arranged by size:
1121 993 722 685 191 160
SfoI GGC'GCC
Cuts at: 1304 1552 1304
Size: 248 3624
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
SmaI CCC'GGG
Cuts at: 894 894
Size: 3872
SmlI C'TyrA_G
Cuts at: 1027 1942 2434 2696 1027
Size: 915 492 262 2203
Fragments arranged by size:
2203 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3872
SphI G_CATG'C
Cuts at: 925 1425 925
Size: 500 3372
StuI AGG'CCT
Cuts at: 460 460
Size: 3872
StyI C'CwwG_G
Cuts at: 1006 1360 1006
Size: 354 3518
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1455 1588 2047 2256 1455
Size: 133 459 209 3071
Fragments arranged by size:
3071 459 209 133
TatI w'GTAC_w
Cuts at: 3387 3387
Size: 3872
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3389 3421 3389
Size: 32 3840
Tth111I GACn'n_nGTC
Cuts at: 1669 1669
Size: 3872
XbaI T'CTAG_A
Cuts at: 384 672 903 384
Size: 288 231 3353
Fragments arranged by size:
3353 288 231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1367 1367
Size: 3872
XmaI C'CCGG_G
Cuts at: 892 892
Size: 3872
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmBI BsrGI BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PspOMI PspXI PsrI SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI