Restriction map for
pF1KB7001
(Circular) (Six-base) MAPSORT of: pF1KB7001.seq Check: 6985 from: 1 to: 7034
pF1KB7001 7034 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 936 1861 2766 936
Size: 925 905 5204
Fragments arranged by size:
5204 925 905
AatII G_ACGT'C
Cuts at: 2812 2812
Size: 7034
AccI GT'mk_AC
Cuts at: 620 4072 620
Size: 3452 3582
Acc65I G'GTAC_C
Cuts at: 4050 4050
Size: 7034
AclI AA'CG_TT
Cuts at: 6874 6874
Size: 7034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 2630 3584 3658 3954 4859 5291 6038 6306
Size: 1882 954 74 296 905 432 747 268
Cuts at: 6306 748
Size: 1476
Fragments arranged by size:
1882 1476 954 905 747 432 296 268
74
AflIII A'CryG_T
Cuts at: 1413 3140 4386 5490 6467 1413
Size: 1727 1246 1104 977 1980
Fragments arranged by size:
1980 1727 1246 1104 977
AgeI A'CCGG_T
Cuts at: 5422 5422
Size: 7034
AleI CACnn'nnGTG
Cuts at: 1546 1546
Size: 7034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 886 1258 1564 886
Size: 372 306 6356
Fragments arranged by size:
6356 372 306
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3487 3519 3487
Size: 32 7002
AlwNI CAG_nnn'CTG
Cuts at: 485 3008 4339 5906 485
Size: 2523 1331 1567 1613
Fragments arranged by size:
2523 1613 1567 1331
ApaI G_GGCC'C
Cuts at: 3086 3086
Size: 7034
ApaLI G'TGCA_C
Cuts at: 523 5804 523
Size: 5281 1753
ApoI r'AATT_y
Cuts at: 1040 1713 2539 3954 4038 6387 6643 1040
Size: 673 826 1415 84 2349 256 1431
Fragments arranged by size:
2349 1431 1415 826 673 256 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 7034
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7034
AvaI C'yCGr_G
Cuts at: 1347 2030 4054 1347
Size: 683 2024 4327
Fragments arranged by size:
4327 2024 683
AvrII C'CTAG_G
Cuts at: 2889 2889
Size: 7034
BamHI G'GATC_C
Cuts at: 4059 4059
Size: 7034
BanI G'GyrC_C
Cuts at: 1255 1586 1846 1895 2228 3255 4050 4464 4712
Size: 331 260 49 333 1027 795 414 248
Cuts at: 4712 4747 1255
Size: 35 3542
Fragments arranged by size:
3542 1027 795 414 333 331 260 248
49 35
BanII G_rGCy'C
Cuts at: 555 1277 1348 3086 4048 5078 555
Size: 722 71 1738 962 1030 2511
Fragments arranged by size:
2511 1738 1030 962 722 71
BbeI G_GCGC'C
Cuts at: 4468 4716 4468
Size: 248 6786
BbsI GAAGACnn'nnnn_
Cuts at: 482 3599 3781 482
Size: 3117 182 3735
Fragments arranged by size:
3735 3117 182
BbvCI CC'TCA_GC
Cuts at: 3189 3189
Size: 7034
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 594 628 985 1019 3898 3932 594
Size: 34 357 34 2879 34 3696
Fragments arranged by size:
3696 2879 357 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4932 5693 7034 4932
Size: 761 1341 4932
Fragments arranged by size:
4932 1341 761
BclI T'GATC_A
Cuts at: 4554 4554
Size: 7034
BfrBI ATG'CAT
Cuts at: 1094 2897 3602 1094
Size: 1803 705 4526
Fragments arranged by size:
4526 1803 705
BglII A'GATC_T
Cuts at: 1157 1991 4549 6586 1157
Size: 834 2558 2037 1605
Fragments arranged by size:
2558 2037 1605 834
BlpI GC'TnA_GC
Cuts at: 859 1363 4146 859
Size: 504 2783 3747
Fragments arranged by size:
3747 2783 504
Bme1580I G_kGCm'C
Cuts at: 527 575 1211 1591 3086 4659 4752 5808 527
Size: 48 636 380 1495 1573 93 1056 1753
Fragments arranged by size:
1753 1573 1495 1056 636 380 93 48
BmgBI CAC'GTC
Cuts at: 3128 3128
Size: 7034
BmrI ACTGGGnnnn_n'
Cuts at: 420 584 1791 1960 2247 3254 4246 4327 4662
Size: 164 1207 169 287 1007 992 81 335
Cuts at: 4662 6806 420
Size: 2144 648
Fragments arranged by size:
2144 1207 1007 992 648 335 287 169
164 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5401 5401
Size: 7034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 325 790 1212 1390 1517 1945 2122 2981
Size: 19 465 422 178 127 428 177 859
Cuts at: 2981 306
Size: 4359
Fragments arranged by size:
4359 859 465 428 422 178 177 127
19
Bpu10I CC'TnA_GC
Cuts at: 3189 3189
Size: 7034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2743 4210 5089 5581 5879 2743
Size: 1467 879 492 298 3898
Fragments arranged by size:
3898 1467 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 779 2758 6738 779
Size: 1979 3980 1075
Fragments arranged by size:
3980 1979 1075
BsaAI yAC'GTr
Cuts at: 4387 4387
Size: 7034
BsaBI GATnn'nnATC
Cuts at: 6 2398 3856 4548 6585 6
Size: 2392 1458 692 2037 455
Fragments arranged by size:
2392 2037 1458 692 455
BsaHI Gr'CG_yC
Cuts at: 2809 4465 4713 5415 6911 2809
Size: 1656 248 702 1496 2932
Fragments arranged by size:
2932 1656 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2584 3492 4223 4744 5422 5696 5843 6254 2584
Size: 908 731 521 678 274 147 411 3364
Fragments arranged by size:
3364 908 731 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 323 353 740 770 2430 2460 323
Size: 30 387 30 1660 30 4897
Fragments arranged by size:
4897 1660 387 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2830 2830
Size: 7034
BseYI C'CCAG_C
Cuts at: 270 312 4459 5217 5794 6290 270
Size: 42 4147 758 577 496 1014
Fragments arranged by size:
4147 1014 758 577 496 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1570 2026 1570
Size: 456 6578
BsiEI CG_ry'CG
Cuts at: 104 4576 4622 5830 104
Size: 4472 46 1208 1308
Fragments arranged by size:
4472 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 527 2129 2800 4048 4826 5016 5808 527
Size: 1602 671 1248 778 190 792 1753
Fragments arranged by size:
1753 1602 1248 792 778 671 190
BsiWI C'GTAC_G
Cuts at: 3919 3919
Size: 7034
BsmI GAATG_Cn'
Cuts at: 513 2897 3738 3750 513
Size: 2384 841 12 3797
Fragments arranged by size:
3797 2384 841 12
BsmBI CGTCTCn'nnnn_
Cuts at: 991 1137 2493 2800 991
Size: 146 1356 307 5225
Fragments arranged by size:
5225 1356 307 146
Bsp1286I G_dGCh'C
Cuts at: 527 555 575 1211 1277 1348 1591 2129 2800
Size: 28 20 636 66 71 243 538 671
Cuts at: 2800 3086 4048 4659 4752 4826 5016 5078 5808
Size: 286 962 611 93 74 190 62 730
Cuts at: 5808 527
Size: 1753
Fragments arranged by size:
1753 962 730 671 636 611 538 286
243 190 93 74 71 66 62 28
20
BspEI T'CCGG_A
Cuts at: 2584 3492 6254 2584
Size: 908 2762 3364
Fragments arranged by size:
3364 2762 908
BspHI T'CATG_A
Cuts at: 3881 6210 3881
Size: 2329 4705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 936 1861 2766 4084 4298 4600 4981 936
Size: 925 905 1318 214 302 381 2989
Fragments arranged by size:
2989 1318 925 905 381 302 214
BsrBI CCG'CTC
Cuts at: 50 933 3786 5326 5380 6864 7029 50
Size: 883 2853 1540 54 1484 165 55
Fragments arranged by size:
2853 1540 1484 883 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1179 3227 4946 1179
Size: 2048 1719 3267
Fragments arranged by size:
3267 2048 1719
BsrFI r'CCGG_y
Cuts at: 1912 5032 5213 5422 1912
Size: 3120 181 209 3524
Fragments arranged by size:
3524 3120 209 181
BsrGI T'GTAC_A
Cuts at: 677 1889 677
Size: 1212 5822
BssSI C'ACGA_G
Cuts at: 1390 5305 5663 1390
Size: 3915 358 2761
Fragments arranged by size:
3915 2761 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3008 4411 3008
Size: 1403 5631
BstBI TT'CG_AA
Cuts at: 1296 3810 5395 1296
Size: 2514 1585 2935
Fragments arranged by size:
2935 2514 1585
BstEII G'GTnAC_C
Cuts at: 2464 2464
Size: 7034
BstXI CCAn_nnnn'nTGG
Cuts at: 1270 2073 2201 3456 1270
Size: 803 128 1255 4848
Fragments arranged by size:
4848 1255 803 128
BstYI r'GATC_y
Cuts at: 1 1157 1991 3762 4059 4526 4549 4884 5130
Size: 1156 834 1771 297 467 23 335 246
Cuts at: 5130 6142 6228 6240 6586 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1771 1156 1012 834 467 449 346 335
297 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 121 765 1086 2066 3584 3681 3743 107
Size: 14 644 321 980 1518 97 62 3398
Fragments arranged by size:
3398 1518 980 644 321 97 62 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4984 5165 5328 4984
Size: 181 163 6690
Fragments arranged by size:
6690 181 163
BtsI GCAGTG_nn'
Cuts at: 383 1833 2151 2272 2985 3743 3851 4277 383
Size: 1450 318 121 713 758 108 426 3140
Fragments arranged by size:
3140 1450 758 713 426 318 121 108
ClaI AT'CG_AT
Cuts at: 2666 2666
Size: 7034
DraI TTT'AAA
Cuts at: 1073 1619 2568 4033 1073
Size: 546 949 1465 4074
Fragments arranged by size:
4074 1465 949 546
DraIII CAC_nnn'GTG
Cuts at: 546 546
Size: 7034
DrdI GACnn_nn'nnGTC
Cuts at: 4740 5598 4740
Size: 858 6176
EaeI y'GGCC_r
Cuts at: 380 660 2982 4619 4793 5184 5211 6556 380
Size: 280 2322 1637 174 391 27 1345 858
Fragments arranged by size:
2322 1637 1345 858 391 280 174 27
EagI C'GGCC_G
Cuts at: 4619 4619
Size: 7034
EarI CTCTTCn'nnn_
Cuts at: 2717 2933 3432 5057 5267 2717
Size: 216 499 1625 210 4484
Fragments arranged by size:
4484 1625 499 216 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5242 5552 5698 6844 5242
Size: 310 146 1146 5432
Fragments arranged by size:
5432 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4046 4046
Size: 7034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 325 748 790 1212 1390 1517 1945 2122
Size: 19 423 42 422 178 127 428 177
Cuts at: 2122 2630 2981 3584 3658 3954 4859 5291 6038
Size: 508 351 603 74 296 905 432 747
Cuts at: 6038 6306 306
Size: 268 1034
Fragments arranged by size:
1034 905 747 603 508 432 428 423
422 351 296 268 178 177 127 74
42 19
EcoNI CCTnn'n_nnAGG
Cuts at: 3077 3352 3077
Size: 275 6759
EcoO109I rG'GnC_Cy
Cuts at: 756 1064 1680 1954 2162 3082 3083 3686 4173
Size: 308 616 274 208 920 1 603 487
Cuts at: 4173 756
Size: 3617
Fragments arranged by size:
3617 920 616 603 487 308 274 208
1
EcoRI G'AATT_C
Cuts at: 1040 2539 4038 1040
Size: 1499 1499 4036
Fragments arranged by size:
4036 1499 1499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1098 2249 2660 5053 1098
Size: 1151 411 2393 3079
Fragments arranged by size:
3079 2393 1151 411
FspI TGC'GCA
Cuts at: 4815 4815
Size: 7034
HaeII r_GCGC'y
Cuts at: 2045 4468 4716 5738 6717 2045
Size: 2423 248 1022 979 2362
Fragments arranged by size:
2423 2362 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 602 634 690 706 722 738 740 772 776
Size: 32 56 16 16 16 2 32 4
Cuts at: 776 808 1261 1293 2430 2462 4876 4906 4908
Size: 32 453 32 1137 32 2414 30 2
Cuts at: 4908 4938 602
Size: 30 2698
Fragments arranged by size:
2698 2414 1137 453 56 32 32 32
32 32 30 30 16 16 16 4
2 2
HincII GTy'rAC
Cuts at: 1224 4073 1224
Size: 2849 4185
HindIII A'AGCT_T
Cuts at: 3327 3327
Size: 7034
KasI G'GCGC_C
Cuts at: 4464 4712 4464
Size: 248 6786
KpnI G_GTAC'C
Cuts at: 4054 4054
Size: 7034
MfeI C'AATT_G
Cuts at: 2316 2316
Size: 7034
MluI A'CGCG_T
Cuts at: 1413 6467 1413
Size: 5054 1980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 273 2792 5705 5889 273
Size: 2519 2913 184 1418
Fragments arranged by size:
2913 2519 1418 184
MscI TGG'CCA
Cuts at: 382 4795 382
Size: 4413 2621
MslI CAynn'nnrTG
Cuts at: 1546 2915 3454 5023 5150 1546
Size: 1369 539 1569 127 3430
Fragments arranged by size:
3430 1569 1369 539 127
MspA1I CmG'CkG
Cuts at: 1232 1943 2100 2838 3704 4145 4459 4819 5832
Size: 711 157 738 866 441 314 360 1013
Cuts at: 5832 6077 1232
Size: 245 2189
Fragments arranged by size:
2189 1013 866 738 711 441 360 314
245 157
NaeI GCC'GGC
Cuts at: 1914 5215 1914
Size: 3301 3733
NarI GG'CG_CC
Cuts at: 4465 4713 4465
Size: 248 6786
NcoI C'CATG_G
Cuts at: 107 765 1086 2066 3584 3681 107
Size: 658 321 980 1518 97 3460
Fragments arranged by size:
3460 1518 980 658 321 97
NgoMIV G'CCGG_C
Cuts at: 1912 5213 1912
Size: 3301 3733
NsiI A_TGCA'T
Cuts at: 1096 2899 3604 1096
Size: 1803 705 4526
Fragments arranged by size:
4526 1803 705
NspI r_CATG'y
Cuts at: 744 3144 3606 4087 4587 5494 744
Size: 2400 462 481 500 907 2284
Fragments arranged by size:
2400 2284 907 500 481 462
PasI CC'CwG_GG
Cuts at: 885 1707 885
Size: 822 6212
PciI A'CATG_T
Cuts at: 3140 5490 3140
Size: 2350 4684
PfoI T'CCnGG_A
Cuts at: 896 1801 896
Size: 905 6129
PmeI GTTT'AAAC
Cuts at: 4033 4033
Size: 7034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2505 6173 6205 2473
Size: 32 3668 32 3302
Fragments arranged by size:
3668 3302 32 32
PpuMI rG'GwC_Cy
Cuts at: 756 1064 1680 1954 2162 3686 756
Size: 308 616 274 208 1524 4104
Fragments arranged by size:
4104 1524 616 308 274 208
PsiI TTA'TAA
Cuts at: 3482 3514 3482
Size: 32 7002
PspOMI G'GGCC_C
Cuts at: 3082 3082
Size: 7034
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2181 2213 2181
Size: 32 7002
PstI C_TGCA'G
Cuts at: 1828 2158 2777 2837 2992 4081 4766 1828
Size: 330 619 60 155 1089 685 4096
Fragments arranged by size:
4096 1089 685 619 330 155 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7034
PvuII CAG'CTG
Cuts at: 2100 2838 3704 4459 4819 2100
Size: 738 866 755 360 4315
Fragments arranged by size:
4315 866 755 738 360
RsrII CG'GwC_CG
Cuts at: 5229 5229
Size: 7034
SacI G_AGCT'C
Cuts at: 4048 4048
Size: 7034
SalI G'TCGA_C
Cuts at: 4071 4071
Size: 7034
SapI GCTCTTCn'nnn_
Cuts at: 5057 5267 5057
Size: 210 6824
SbfI CC_TGCA'GG
Cuts at: 2777 4081 2777
Size: 1304 5730
ScaI AGT'ACT
Cuts at: 705 6551 705
Size: 5846 1188
SexAI A'CCwGG_T
Cuts at: 2200 2200
Size: 7034
SfcI C'TryA_G
Cuts at: 33 1824 2154 2773 2833 2988 4077 4762 5755
Size: 1791 330 619 60 155 1089 685 993
Cuts at: 5755 5946 33
Size: 191 1121
Fragments arranged by size:
1791 1121 1089 993 685 619 330 191
155 60
SfoI GGC'GCC
Cuts at: 4466 4714 4466
Size: 248 6786
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7034
SmaI CCC'GGG
Cuts at: 4056 4056
Size: 7034
SmlI C'TyrA_G
Cuts at: 2758 4189 5104 5596 5858 2758
Size: 1431 915 492 262 3934
Fragments arranged by size:
3934 1431 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7034
SphI G_CATG'C
Cuts at: 744 4087 4587 744
Size: 3343 500 3191
Fragments arranged by size:
3343 3191 500
SspI AAT'ATT
Cuts at: 4021 4021
Size: 7034
StyI C'CwwG_G
Cuts at: 107 765 797 989 1086 1638 1676 2066 2072
Size: 658 32 192 97 552 38 390 6
Cuts at: 2072 2791 2889 3218 3527 3584 3681 4168 4522
Size: 719 98 329 309 57 97 487 354
Cuts at: 4522 107
Size: 2619
Fragments arranged by size:
2619 719 658 552 487 390 354 329
309 192 98 97 97 57 38 32
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 402 829 909 1536 2483 3273 4617 4750 5209
Size: 427 80 627 947 790 1344 133 459
Cuts at: 5209 5418 402
Size: 209 2018
Fragments arranged by size:
2018 1344 947 790 627 459 427 209
133 80
TatI w'GTAC_w
Cuts at: 464 677 703 1889 1967 3617 6549 464
Size: 213 26 1186 78 1650 2932 949
Fragments arranged by size:
2932 1650 1186 949 213 78 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 576 608 965 997 1704 1736 6551 6583 576
Size: 32 357 32 707 32 4815 32 1027
Fragments arranged by size:
4815 1027 707 357 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4831 4831
Size: 7034
XbaI T'CTAG_A
Cuts at: 4065 4065
Size: 7034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1541 2201 4529 1541
Size: 660 2328 4046
Fragments arranged by size:
4046 2328 660
XmaI C'CCGG_G
Cuts at: 4054 4054
Size: 7034
XmnI GAAnn'nnTTC
Cuts at: 1081 2884 3816 1081
Size: 1803 932 4299
Fragments arranged by size:
4299 1803 932
ZraI GAC'GTC
Cuts at: 2810 2810
Size: 7034
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PfoI PmeI PpiI PpuMI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI BaeI BglI BmtI BssHII
BstZ17I Bsu36I CspCI EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI PacI PflMI PmlI PshAI PspXI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI