Restriction map for
pF1KB7006
(Circular) (Six-base) MAPSORT of: pF1KB7006.seq Check: 4470 from: 1 to: 6311
pF1KB7006 6311 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 3349 3349
Size: 6311
Acc65I G'GTAC_C
Cuts at: 2932 3299 3327 2932
Size: 367 28 5916
Fragments arranged by size:
5916 367 28
AclI AA'CG_TT
Cuts at: 6151 6151
Size: 6311
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 917 1367 2267 2726 3251 4136 4568 5315
Size: 541 450 900 459 525 885 432 747
Cuts at: 5315 5583 376
Size: 268 1104
Fragments arranged by size:
1104 900 885 747 541 525 459 450
432 268
AflIII A'CryG_T
Cuts at: 3663 4767 5744 3663
Size: 1104 977 4230
Fragments arranged by size:
4230 1104 977
AgeI A'CCGG_T
Cuts at: 1146 4699 1146
Size: 3553 2758
AleI CACnn'nnGTG
Cuts at: 1611 1611
Size: 6311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1274 2905 1274
Size: 1631 4680
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2425 2457 2743 2775 2425
Size: 32 286 32 5961
Fragments arranged by size:
5961 286 32 32
AlwNI CAG_nnn'CTG
Cuts at: 415 1185 1524 2352 3262 3616 5183 415
Size: 770 339 828 910 354 1567 1543
Fragments arranged by size:
1567 1543 910 828 770 354 339
ApaLI G'TGCA_C
Cuts at: 3021 5081 3021
Size: 2060 4251
ApoI r'AATT_y
Cuts at: 527 2424 3248 3315 5664 5920 527
Size: 1897 824 67 2349 256 918
Fragments arranged by size:
2349 1897 918 824 256 67
AseI AT'TA_AT
Cuts at: 20 830 20
Size: 810 5501
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6311
AvaI C'yCGr_G
Cuts at: 419 1484 2111 3331 419
Size: 1065 627 1220 3399
Fragments arranged by size:
3399 1220 1065 627
AvrII C'CTAG_G
Cuts at: 2725 2725
Size: 6311
BamHI G'GATC_C
Cuts at: 1138 2393 2433 2576 3336 1138
Size: 1255 40 143 760 4113
Fragments arranged by size:
4113 1255 760 143 40
BanI G'GyrC_C
Cuts at: 1950 2932 3299 3327 3741 3989 4024 1950
Size: 982 367 28 414 248 35 4237
Fragments arranged by size:
4237 982 414 367 248 35 28
BanII G_rGCy'C
Cuts at: 653 3325 4355 653
Size: 2672 1030 2609
Fragments arranged by size:
2672 2609 1030
BbeI G_GCGC'C
Cuts at: 1954 3745 3993 1954
Size: 1791 248 4272
Fragments arranged by size:
4272 1791 248
BbsI GAAGACnn'nnnn_
Cuts at: 1461 2110 1461
Size: 649 5662
BbvCI CC'TCA_GC
Cuts at: 494 494
Size: 6311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6277
BciVI GTATCCnnnnn_n'
Cuts at: 4209 4970 6311 4209
Size: 761 1341 4209
Fragments arranged by size:
4209 1341 761
BclI T'GATC_A
Cuts at: 1104 3831 1104
Size: 2727 3584
BglI GCCn_nnn'nGGC
Cuts at: 250 1719 1771 2044 250
Size: 1469 52 273 4517
Fragments arranged by size:
4517 1469 273 52
BglII A'GATC_T
Cuts at: 164 1010 1446 1725 3826 5863 164
Size: 846 436 279 2101 2037 612
Fragments arranged by size:
2101 2037 846 612 436 279
BlpI GC'TnA_GC
Cuts at: 1166 3423 1166
Size: 2257 4054
Bme1580I G_kGCm'C
Cuts at: 2946 3025 3936 4029 5085 2946
Size: 79 911 93 1056 4172
Fragments arranged by size:
4172 1056 911 93 79
BmrI ACTGGGnnnn_n'
Cuts at: 3523 3604 3939 6083 3523
Size: 81 335 2144 3751
Fragments arranged by size:
3751 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4678 4678
Size: 6311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1460 166
Size: 1294 5017
Bpu10I CC'TnA_GC
Cuts at: 494 2765 494
Size: 2271 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 3487 4366 4858 5156 1606
Size: 1881 879 492 298 2761
Fragments arranged by size:
2761 1881 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2139 2188 6015 2139
Size: 49 3827 2435
Fragments arranged by size:
3827 2435 49
BsaAI yAC'GTr
Cuts at: 3664 3664
Size: 6311
BsaBI GATnn'nnATC
Cuts at: 6 3825 5862 6
Size: 3819 2037 455
Fragments arranged by size:
3819 2037 455
BsaHI Gr'CG_yC
Cuts at: 1951 3742 3990 4692 6188 1951
Size: 1791 248 702 1496 2074
Fragments arranged by size:
2074 1791 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1146 1311 2390 3500 4021 4699 4973 5120 5531
Size: 165 1079 1110 521 678 274 147 411
Cuts at: 5531 1146
Size: 1926
Fragments arranged by size:
1926 1110 1079 678 521 411 274 165
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1902 1932 1938 1968 1902
Size: 30 6 30 6245
Fragments arranged by size:
6245 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 2675 3163 205
Size: 2470 488 3353
Fragments arranged by size:
3353 2470 488
BseYI C'CCAG_C
Cuts at: 1766 2359 3086 3123 3278 3736 4494 5071 5567
Size: 593 727 37 155 458 758 577 496
Cuts at: 5567 1766
Size: 2510
Fragments arranged by size:
2510 758 727 593 577 496 458 155
37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1067 268
Size: 799 5512
BsiEI CG_ry'CG
Cuts at: 104 134 257 2039 3853 3899 5107 104
Size: 30 123 1782 1814 46 1208 1308
Fragments arranged by size:
1814 1782 1308 1208 123 46 30
BsiHKAI G_wGCw'C
Cuts at: 653 668 1196 1821 3025 3325 4103 4293 5085
Size: 15 528 625 1204 300 778 190 792
Cuts at: 5085 653
Size: 1879
Fragments arranged by size:
1879 1204 792 778 625 528 300 190
15
BsmI GAATG_Cn'
Cuts at: 360 2087 360
Size: 1727 4584
Bsp1286I G_dGCh'C
Cuts at: 653 668 1196 1821 2946 3025 3325 3936 4029
Size: 15 528 625 1125 79 300 611 93
Cuts at: 4029 4103 4293 4355 5085 653
Size: 74 190 62 730 1879
Fragments arranged by size:
1879 1125 730 625 611 528 300 190
93 79 74 62 15
BspEI T'CCGG_A
Cuts at: 5531 5531
Size: 6311
BspHI T'CATG_A
Cuts at: 5487 5487
Size: 6311
BspMI ACCTGCnnnn'nnnn_
Cuts at: 479 1161 1532 3361 3575 3877 4258 479
Size: 682 371 1829 214 302 381 2532
Fragments arranged by size:
2532 1829 682 381 371 302 214
BsrBI CCG'CTC
Cuts at: 50 4603 4657 6141 6306 50
Size: 4553 54 1484 165 55
Fragments arranged by size:
4553 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1256 2862 2864 4223 1256
Size: 1606 2 1359 3344
Fragments arranged by size:
3344 1606 1359 2
BsrFI r'CCGG_y
Cuts at: 150 1146 1563 4309 4490 4699 150
Size: 996 417 2746 181 209 1762
Fragments arranged by size:
2746 1762 996 417 209 181
BsrGI T'GTAC_A
Cuts at: 1578 1578
Size: 6311
BssSI C'ACGA_G
Cuts at: 187 1814 4582 4940 187
Size: 1627 2768 358 1558
Fragments arranged by size:
2768 1627 1558 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1524 3688 1524
Size: 2164 4147
BstBI TT'CG_AA
Cuts at: 3252 4672 3252
Size: 1420 4891
BstXI CCAn_nnnn'nTGG
Cuts at: 1503 2883 3123 1503
Size: 1380 240 4691
Fragments arranged by size:
4691 1380 240
BstYI r'GATC_y
Cuts at: 1 164 1010 1138 1296 1446 1725 1851 2393
Size: 163 846 128 158 150 279 126 542
Cuts at: 2393 2433 2576 3336 3803 3826 4161 4407 5419
Size: 40 143 760 467 23 335 246 1012
Cuts at: 5419 5505 5517 5863 1
Size: 86 12 346 449
Fragments arranged by size:
1012 846 760 542 467 449 346 335
279 246 163 158 150 143 128 126
86 40 23 12
Bsu36I CC'TnA_GG
Cuts at: 1808 2618 1808
Size: 810 5501
BtgI C'CryG_G
Cuts at: 236 1508 1641 1946 236
Size: 1272 133 305 4601
Fragments arranged by size:
4601 1272 305 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2952 4261 4442 4605 2952
Size: 1309 181 163 4658
Fragments arranged by size:
4658 1309 181 163
BtsI GCAGTG_nn'
Cuts at: 1943 2934 3025 3271 3554 1943
Size: 991 91 246 283 4700
Fragments arranged by size:
4700 991 283 246 91
ClaI AT'CG_AT
Cuts at: 1271 2216 1271
Size: 945 5366
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2498 2533 2498
Size: 35 6276
DraI TTT'AAA
Cuts at: 788 3310 788
Size: 2522 3789
DraIII CAC_nnn'GTG
Cuts at: 177 672 177
Size: 495 5816
DrdI GACnn_nn'nnGTC
Cuts at: 1645 4017 4875 1645
Size: 2372 858 3081
Fragments arranged by size:
3081 2372 858
EaeI y'GGCC_r
Cuts at: 239 254 620 1151 1493 1505 1550 1565 1676
Size: 15 366 531 342 12 45 15 111
Cuts at: 1676 2036 2330 2591 2999 3896 4070 4461 4488
Size: 360 294 261 408 897 174 391 27
Cuts at: 4488 5833 239
Size: 1345 717
Fragments arranged by size:
1345 897 717 531 408 391 366 360
342 294 261 174 111 45 27 15
15 12
EagI C'GGCC_G
Cuts at: 254 2036 3896 254
Size: 1782 1860 2669
Fragments arranged by size:
2669 1860 1782
EarI CTCTTCn'nnn_
Cuts at: 313 901 1316 1970 2750 4334 4544 313
Size: 588 415 654 780 1584 210 2080
Fragments arranged by size:
2080 1584 780 654 588 415 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4519 4829 4975 6121 4519
Size: 310 146 1146 4709
Fragments arranged by size:
4709 1146 310 146
EcoICRI GAG'CTC
Cuts at: 651 3323 651
Size: 2672 3639
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 376 917 1367 1460 2267 2726 3251 4136
Size: 210 541 450 93 807 459 525 885
Cuts at: 4136 4568 5315 5583 166
Size: 432 747 268 894
Fragments arranged by size:
894 885 807 747 541 525 459 450
432 268 210 93
EcoNI CCTnn'n_nnAGG
Cuts at: 3052 3052
Size: 6311
EcoO109I rG'GnC_Cy
Cuts at: 1711 2715 3450 1711
Size: 1004 735 4572
Fragments arranged by size:
4572 1004 735
EcoRI G'AATT_C
Cuts at: 2424 3315 2424
Size: 891 5420
EcoRV GAT'ATC
Cuts at: 2572 2572
Size: 6311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4330 4330
Size: 6311
FspI TGC'GCA
Cuts at: 4092 4092
Size: 6311
HaeII r_GCGC'y
Cuts at: 1954 3745 3993 5015 5994 1954
Size: 1791 248 1022 979 2271
Fragments arranged by size:
2271 1791 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 391 423 1089 1121 1608 1640 2251 2283 2561
Size: 32 666 32 487 32 611 32 278
Cuts at: 2561 2593 3136 3168 4153 4183 4185 4215 391
Size: 32 543 32 985 30 2 30 2487
Fragments arranged by size:
2487 985 666 611 543 487 278 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3350 3350
Size: 6311
HindIII A'AGCT_T
Cuts at: 367 782 2023 367
Size: 415 1241 4655
Fragments arranged by size:
4655 1241 415
KasI G'GCGC_C
Cuts at: 1950 3741 3989 1950
Size: 1791 248 4272
Fragments arranged by size:
4272 1791 248
KpnI G_GTAC'C
Cuts at: 2936 3303 3331 2936
Size: 367 28 5916
Fragments arranged by size:
5916 367 28
MluI A'CGCG_T
Cuts at: 5744 5744
Size: 6311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 258 2255 2558 2637 2688 4982 5166 258
Size: 1997 303 79 51 2294 184 1403
Fragments arranged by size:
2294 1997 1403 303 184 79 51
MscI TGG'CCA
Cuts at: 622 1153 1495 1507 1552 2332 2593 3001 4072
Size: 531 342 12 45 780 261 408 1071
Cuts at: 4072 622
Size: 2861
Fragments arranged by size:
2861 1071 780 531 408 342 261 45
12
MslI CAynn'nnrTG
Cuts at: 112 1611 3169 4300 4427 112
Size: 1499 1558 1131 127 1996
Fragments arranged by size:
1996 1558 1499 1131 127
MspA1I CmG'CkG
Cuts at: 1576 1611 1758 1770 3422 3736 4096 5109 5354
Size: 35 147 12 1652 314 360 1013 245
Cuts at: 5354 1576
Size: 2533
Fragments arranged by size:
2533 1652 1013 360 314 245 147 35
12
NaeI GCC'GGC
Cuts at: 4492 4492
Size: 6311
NarI GG'CG_CC
Cuts at: 1951 3742 3990 1951
Size: 1791 248 4272
Fragments arranged by size:
4272 1791 248
NgoMIV G'CCGG_C
Cuts at: 4490 4490
Size: 6311
NspI r_CATG'y
Cuts at: 3364 3864 4771 3364
Size: 500 907 4904
Fragments arranged by size:
4904 907 500
PasI CC'CwG_GG
Cuts at: 979 979
Size: 6311
PciI A'CATG_T
Cuts at: 4767 4767
Size: 6311
PflMI CCAn_nnn'nTGG
Cuts at: 953 1306 953
Size: 353 5958
PfoI T'CCnGG_A
Cuts at: 143 2558 143
Size: 2415 3896
PmeI GTTT'AAAC
Cuts at: 3310 3310
Size: 6311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5450 5482 5450
Size: 32 6279
PpuMI rG'GwC_Cy
Cuts at: 2715 2715
Size: 6311
PspXI vC'TCGA_Gb
Cuts at: 1484 1484
Size: 6311
PstI C_TGCA'G
Cuts at: 1520 1529 3358 4043 1520
Size: 9 1829 685 3788
Fragments arranged by size:
3788 1829 685 9
PvuI CG_AT'CG
Cuts at: 104 134 104
Size: 30 6281
PvuII CAG'CTG
Cuts at: 1576 1758 1770 3736 4096 1576
Size: 182 12 1966 360 3791
Fragments arranged by size:
3791 1966 360 182 12
RsrII CG'GwC_CG
Cuts at: 4506 4506
Size: 6311
SacI G_AGCT'C
Cuts at: 653 3325 653
Size: 2672 3639
SalI G'TCGA_C
Cuts at: 3348 3348
Size: 6311
SapI GCTCTTCn'nnn_
Cuts at: 1316 4334 4544 1316
Size: 3018 210 3083
Fragments arranged by size:
3083 3018 210
SbfI CC_TGCA'GG
Cuts at: 3358 3358
Size: 6311
ScaI AGT'ACT
Cuts at: 5828 5828
Size: 6311
SexAI A'CCwGG_T
Cuts at: 173 3302 173
Size: 3129 3182
SfcI C'TryA_G
Cuts at: 33 806 1516 1525 3354 4039 5032 5223 33
Size: 773 710 9 1829 685 993 191 1121
Fragments arranged by size:
1829 1121 993 773 710 685 191 9
SfoI GGC'GCC
Cuts at: 1952 3743 3991 1952
Size: 1791 248 4272
Fragments arranged by size:
4272 1791 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6311
SgrAI Cr'CCGG_yG
Cuts at: 1146 1146
Size: 6311
SmaI CCC'GGG
Cuts at: 3333 3333
Size: 6311
SmlI C'TyrA_G
Cuts at: 419 1484 1585 3466 4381 4873 5135 419
Size: 1065 101 1881 915 492 262 1595
Fragments arranged by size:
1881 1595 1065 915 492 262 101
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6311
SphI G_CATG'C
Cuts at: 3364 3864 3364
Size: 500 5811
StuI AGG'CCT
Cuts at: 2095 2730 2977 2095
Size: 635 247 5429
Fragments arranged by size:
5429 635 247
StyI C'CwwG_G
Cuts at: 768 1410 1490 2594 2725 2960 2972 3002 3445
Size: 642 80 1104 131 235 12 30 443
Cuts at: 3445 3799 768
Size: 354 3280
Fragments arranged by size:
3280 1104 642 443 354 235 131 80
30 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1639 1760 3100 3894 4027 4486 4695 1639
Size: 121 1340 794 133 459 209 3255
Fragments arranged by size:
3255 1340 794 459 209 133 121
TatI w'GTAC_w
Cuts at: 1578 5826 1578
Size: 4248 2063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1938 1970 5828 5860 1938
Size: 32 3858 32 2389
Fragments arranged by size:
3858 2389 32 32
Tth111I GACn'n_nGTC
Cuts at: 4108 4108
Size: 6311
XbaI T'CTAG_A
Cuts at: 3342 3342
Size: 6311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1545 1929 2271 2493 3171 3806 1545
Size: 384 342 222 678 635 4050
Fragments arranged by size:
4050 678 635 384 342 222
XhoI C'TCGA_G
Cuts at: 419 1484 419
Size: 1065 5246
XmaI C'CCGG_G
Cuts at: 3331 3331
Size: 6311
XmnI GAAnn'nnTTC
Cuts at: 2087 2529 2087
Size: 442 5869
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI BaeI
BfrBI BmgBI BmtI BsiWI BsmBI BssHII BstEII BstZ17I
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PsrI
SacII SanDI SfiI SnaBI SrfI SspI SwaI ZraI