Restriction map for
pF1KB7008
(Circular) (Six-base) MAPSORT of: pF1KB7008.seq Check: 4903 from: 1 to: 4508
pF1KB7008 4508 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 654 1137 1274 654
Size: 483 137 3888
Fragments arranged by size:
3888 483 137
AatII G_ACGT'C
Cuts at: 440 440
Size: 4508
AccI GT'mk_AC
Cuts at: 1546 1546
Size: 4508
Acc65I G'GTAC_C
Cuts at: 1524 1524
Size: 4508
AclI AA'CG_TT
Cuts at: 4348 4348
Size: 4508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 313 2333 2765 3118 3780 196
Size: 117 2020 432 353 662 924
Fragments arranged by size:
2020 924 662 432 353 117
AflIII A'CryG_T
Cuts at: 1860 3660 3941 1860
Size: 1800 281 2427
Fragments arranged by size:
2427 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 845 845
Size: 4508
AlwNI CAG_nnn'CTG
Cuts at: 738 1813 3251 738
Size: 1075 1438 1995
Fragments arranged by size:
1995 1438 1075
ApaLI G'TGCA_C
Cuts at: 477 3346 477
Size: 2869 1639
ApoI r'AATT_y
Cuts at: 1512 3861 4117 1512
Size: 2349 256 1903
Fragments arranged by size:
2349 1903 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4508
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
AvaI C'yCGr_G
Cuts at: 150 278 1528 150
Size: 128 1250 3130
Fragments arranged by size:
3130 1250 128
BamHI G'GATC_C
Cuts at: 1533 1533
Size: 4508
BanI G'GyrC_C
Cuts at: 502 595 960 1524 1938 2186 2221 502
Size: 93 365 564 414 248 35 2789
Fragments arranged by size:
2789 564 414 365 248 93 35
BanII G_rGCy'C
Cuts at: 458 1039 1522 2552 458
Size: 581 483 1030 2414
Fragments arranged by size:
2414 1030 581 483
BbeI G_GCGC'C
Cuts at: 1942 2190 1942
Size: 248 4260
BbsI GAAGACnn'nnnn_
Cuts at: 328 392 328
Size: 64 4444
BbvCI CC'TCA_GC
Cuts at: 545 784 545
Size: 239 4269
BciVI GTATCCnnnnn_n'
Cuts at: 1013 2406 3462 4508 1013
Size: 1393 1056 1046 1013
Fragments arranged by size:
1393 1056 1046 1013
BclI T'GATC_A
Cuts at: 2028 2028
Size: 4508
BglI GCCn_nnn'nGGC
Cuts at: 295 556 802 928 978 1241 1423 295
Size: 261 246 126 50 263 182 3380
Fragments arranged by size:
3380 263 261 246 182 126 50
BglII A'GATC_T
Cuts at: 2023 4060 2023
Size: 2037 2471
BlpI GC'TnA_GC
Cuts at: 1620 1620
Size: 4508
Bme1580I G_kGCm'C
Cuts at: 279 481 965 1410 2133 2226 3350 279
Size: 202 484 445 723 93 1124 1437
Fragments arranged by size:
1437 1124 723 484 445 202 93
BmgBI CAC'GTC
Cuts at: 1323 1323
Size: 4508
BmrI ACTGGGnnnn_n'
Cuts at: 669 986 1720 1801 2136 4280 669
Size: 317 734 81 335 2144 897
Fragments arranged by size:
2144 897 734 335 317 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 4508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 688 1130 1282 181
Size: 507 442 152 3407
Fragments arranged by size:
3407 507 442 152
Bpu10I CC'TnA_GC
Cuts at: 545 784 545
Size: 239 4269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1684 2563 3277 3575 1684
Size: 879 714 298 2617
Fragments arranged by size:
2617 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 652 4212 652
Size: 3560 948
BsaAI yAC'GTr
Cuts at: 1861 1861
Size: 4508
BsaBI GATnn'nnATC
Cuts at: 6 2022 4059 6
Size: 2016 2037 455
Fragments arranged by size:
2037 2016 455
BsaHI Gr'CG_yC
Cuts at: 437 941 1939 2187 2889 4385 437
Size: 504 998 248 702 1496 560
Fragments arranged by size:
1496 998 702 560 504 248
BsaWI w'CCGG_w
Cuts at: 389 440 1697 2218 2896 3307 3454 3728 389
Size: 51 1257 521 678 411 147 274 1169
Fragments arranged by size:
1257 1169 678 521 411 274 147 51
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 395 178
Size: 217 4291
BseYI C'CCAG_C
Cuts at: 968 1026 1104 1160 1933 2691 3356 3764 968
Size: 58 78 56 773 758 665 408 1712
Fragments arranged by size:
1712 773 758 665 408 78 58 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 295 832 229
Size: 66 537 3905
Fragments arranged by size:
3905 537 66
BsiEI CG_ry'CG
Cuts at: 104 2050 2096 3326 104
Size: 1946 46 1230 1286
Fragments arranged by size:
1946 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 481 488 1522 2300 2490 3350 481
Size: 7 1034 778 190 860 1639
Fragments arranged by size:
1639 1034 860 778 190 7
Bsp1286I G_dGCh'C
Cuts at: 279 458 481 488 965 1039 1410 1522 2133
Size: 179 23 7 477 74 371 112 611
Cuts at: 2133 2226 2300 2490 2552 3350 279
Size: 93 74 190 62 798 1437
Fragments arranged by size:
1437 798 611 477 371 190 179 112
93 74 74 62 23 7
BspEI T'CCGG_A
Cuts at: 440 440
Size: 4508
BspHI T'CATG_A
Cuts at: 2940 2940
Size: 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 654 1137 1274 1558 1772 2074 2455 654
Size: 483 137 284 214 302 381 2707
Fragments arranged by size:
2707 483 381 302 284 214 137
BsrBI CCG'CTC
Cuts at: 50 2800 2854 4338 4503 50
Size: 2750 54 1484 165 55
Fragments arranged by size:
2750 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2420 2420
Size: 4508
BsrFI r'CCGG_y
Cuts at: 1493 2506 2687 1493
Size: 1013 181 3314
Fragments arranged by size:
3314 1013 181
BssSI C'ACGA_G
Cuts at: 2779 3487 2779
Size: 708 3800
BstAPI GCAn_nnn'nTGC
Cuts at: 515 1885 515
Size: 1370 3138
BstBI TT'CG_AA
Cuts at: 188 2869 188
Size: 2681 1827
BstXI CCAn_nnnn'nTGG
Cuts at: 612 678 976 612
Size: 66 298 4144
Fragments arranged by size:
4144 298 66
BstYI r'GATC_y
Cuts at: 1 146 203 1292 1533 2000 2023 2358 2604
Size: 145 57 1089 241 467 23 335 246
Cuts at: 2604 2910 2922 3008 4060 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1089 1052 467 449 335 306 246 241
145 86 57 23 12
Bsu36I CC'TnA_GG
Cuts at: 876 1481 876
Size: 605 3903
BtgI C'CryG_G
Cuts at: 791 800 1040 1049 791
Size: 9 240 9 4250
Fragments arranged by size:
4250 240 9 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 681 837 885 2458 2639 2802 681
Size: 156 48 1573 181 163 2387
Fragments arranged by size:
2387 1573 181 163 156 48
BtsI GCAGTG_nn'
Cuts at: 1751 1751
Size: 4508
DraI TTT'AAA
Cuts at: 1507 1507
Size: 4508
DrdI GACnn_nn'nnGTC
Cuts at: 2214 3558 2214
Size: 1344 3164
EaeI y'GGCC_r
Cuts at: 557 668 794 1317 1400 2093 2267 2658 2685
Size: 111 126 523 83 693 174 391 27
Cuts at: 2685 4030 557
Size: 1345 1035
Fragments arranged by size:
1345 1035 693 523 391 174 126 111
83 27
EagI C'GGCC_G
Cuts at: 2093 2093
Size: 4508
EarI CTCTTCn'nnn_
Cuts at: 2531 2741 2531
Size: 210 4298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 640 2716 3458 3604 4318 640
Size: 2076 742 146 714 830
Fragments arranged by size:
2076 830 742 714 146
EcoICRI GAG'CTC
Cuts at: 1520 1520
Size: 4508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 196 313 688 1130 1282 2333 2765 3118
Size: 15 117 375 442 152 1051 432 353
Cuts at: 3118 3780 181
Size: 662 909
Fragments arranged by size:
1051 909 662 442 432 375 353 152
117 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1063 1270 1063
Size: 207 4301
EcoO109I rG'GnC_Cy
Cuts at: 171 735 1234 1647 171
Size: 564 499 413 3032
Fragments arranged by size:
3032 564 499 413
EcoRI G'AATT_C
Cuts at: 1512 1512
Size: 4508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 201 2527 201
Size: 2326 2182
FspI TGC'GCA
Cuts at: 2289 2289
Size: 4508
HaeII r_GCGC'y
Cuts at: 308 725 1458 1942 2190 3420 4191 308
Size: 417 733 484 248 1230 771 625
Fragments arranged by size:
1230 771 733 625 484 417 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2350 2380 2382 2412 2350
Size: 30 2 30 4446
Fragments arranged by size:
4446 30 30 2
HincII GTy'rAC
Cuts at: 945 1347 1547 945
Size: 402 200 3906
Fragments arranged by size:
3906 402 200
KasI G'GCGC_C
Cuts at: 1938 2186 1938
Size: 248 4260
KpnI G_GTAC'C
Cuts at: 1528 1528
Size: 4508
MluI A'CGCG_T
Cuts at: 3941 3941
Size: 4508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 181 3267 3451 181
Size: 3086 184 1238
Fragments arranged by size:
3086 1238 184
MscI TGG'CCA
Cuts at: 670 796 1319 2269 670
Size: 126 523 950 2909
Fragments arranged by size:
2909 950 523 126
MslI CAynn'nnrTG
Cuts at: 2497 2624 2497
Size: 127 4381
MspA1I CmG'CkG
Cuts at: 1042 1619 1933 2293 3077 3322 1042
Size: 577 314 360 784 245 2228
Fragments arranged by size:
2228 784 577 360 314 245
NaeI GCC'GGC
Cuts at: 2689 2689
Size: 4508
NarI GG'CG_CC
Cuts at: 1939 2187 1939
Size: 248 4260
NcoI C'CATG_G
Cuts at: 791 800 791
Size: 9 4499
NgoMIV G'CCGG_C
Cuts at: 2687 2687
Size: 4508
NspI r_CATG'y
Cuts at: 660 687 1561 2061 3664 660
Size: 27 874 500 1603 1504
Fragments arranged by size:
1603 1504 874 500 27
PasI CC'CwG_GG
Cuts at: 936 936
Size: 4508
PciI A'CATG_T
Cuts at: 3660 3660
Size: 4508
PmeI GTTT'AAAC
Cuts at: 1507 1507
Size: 4508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2954 2986 2954
Size: 32 4476
PpuMI rG'GwC_Cy
Cuts at: 171 171
Size: 4508
PstI C_TGCA'G
Cuts at: 1064 1555 2240 1064
Size: 491 685 3332
Fragments arranged by size:
3332 685 491
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4508
PvuII CAG'CTG
Cuts at: 1933 2293 1933
Size: 360 4148
RsrII CG'GwC_CG
Cuts at: 2703 2703
Size: 4508
SacI G_AGCT'C
Cuts at: 1522 1522
Size: 4508
SacII CC_GC'GG
Cuts at: 1043 1043
Size: 4508
SalI G'TCGA_C
Cuts at: 1545 1545
Size: 4508
SapI GCTCTTCn'nnn_
Cuts at: 2531 2741 2531
Size: 210 4298
SbfI CC_TGCA'GG
Cuts at: 1555 1555
Size: 4508
ScaI AGT'ACT
Cuts at: 4025 4025
Size: 4508
SfcI C'TryA_G
Cuts at: 33 717 951 1060 1113 1551 2236 3204 3395
Size: 684 234 109 53 438 685 968 191
Cuts at: 3395 33
Size: 1146
Fragments arranged by size:
1146 968 685 684 438 234 191 109
53
SfiI GGCCn_nnn'nGGCC
Cuts at: 556 556
Size: 4508
SfoI GGC'GCC
Cuts at: 1940 2188 1940
Size: 248 4260
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
SmaI CCC'GGG
Cuts at: 280 1530 280
Size: 1250 3258
SmlI C'TyrA_G
Cuts at: 1663 2578 3292 3554 1663
Size: 915 714 262 2617
Fragments arranged by size:
2617 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4508
SphI G_CATG'C
Cuts at: 660 1561 2061 660
Size: 901 500 3107
Fragments arranged by size:
3107 901 500
StuI AGG'CCT
Cuts at: 1069 1069
Size: 4508
StyI C'CwwG_G
Cuts at: 791 800 1642 1996 791
Size: 9 842 354 3303
Fragments arranged by size:
3303 842 354 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 688 745 982 1364 2091 2224 2683 2892 688
Size: 57 237 382 727 133 459 209 2304
Fragments arranged by size:
2304 727 459 382 237 209 133 57
TatI w'GTAC_w
Cuts at: 233 4023 233
Size: 3790 718
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1118 1150 4025 4057 1118
Size: 32 2875 32 1569
Fragments arranged by size:
2875 1569 32 32
Tth111I GACn'n_nGTC
Cuts at: 2305 2305
Size: 4508
XbaI T'CTAG_A
Cuts at: 1539 1539
Size: 4508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 798 1429 2003 798
Size: 631 574 3303
Fragments arranged by size:
3303 631 574
XmaI C'CCGG_G
Cuts at: 278 1528 278
Size: 1250 3258
XmnI GAAnn'nnTTC
Cuts at: 1435 1435
Size: 4508
ZraI GAC'GTC
Cuts at: 438 438
Size: 4508
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI