Restriction map for
pF1KB7009
(Circular) (Six-base) MAPSORT of: pF1KB7009.seq Check: 1379 from: 1 to: 6329
pF1KB7009 6329 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1600 1600
Size: 6329
AccI GT'mk_AC
Cuts at: 949 2057 3367 949
Size: 1108 1310 3911
Fragments arranged by size:
3911 1310 1108
Acc65I G'GTAC_C
Cuts at: 898 3288 3345 898
Size: 2390 57 3882
Fragments arranged by size:
3882 2390 57
AclI AA'CG_TT
Cuts at: 6169 6169
Size: 6329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 2683 2917 4154 4586 5333 5601 1285
Size: 1398 234 1237 432 747 268 2013
Fragments arranged by size:
2013 1398 1237 747 432 268 234
AfeI AGC'GCT
Cuts at: 3202 3202
Size: 6329
AflIII A'CryG_T
Cuts at: 3681 4785 5762 3681
Size: 1104 977 4248
Fragments arranged by size:
4248 1104 977
AgeI A'CCGG_T
Cuts at: 4717 4717
Size: 6329
AleI CACnn'nnGTG
Cuts at: 1660 1913 1660
Size: 253 6076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 560 1043 1306 1328 1678 560
Size: 483 263 22 350 5211
Fragments arranged by size:
5211 483 350 263 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1104 1136 2476 2508 1104
Size: 32 1340 32 4925
Fragments arranged by size:
4925 1340 32 32
AlwNI CAG_nnn'CTG
Cuts at: 627 813 879 1207 1523 2034 2247 2807 2940
Size: 186 66 328 316 511 213 560 133
Cuts at: 2940 3001 3276 3634 5201 627
Size: 61 275 358 1567 1755
Fragments arranged by size:
1755 1567 560 511 358 328 316 275
213 186 133 66 61
ApaI G_GGCC'C
Cuts at: 1490 1932 2223 2991 1490
Size: 442 291 768 4828
Fragments arranged by size:
4828 768 442 291
ApaLI G'TGCA_C
Cuts at: 2262 5099 2262
Size: 2837 3492
ApoI r'AATT_y
Cuts at: 679 3333 5682 5938 679
Size: 2654 2349 256 1070
Fragments arranged by size:
2654 2349 1070 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6329
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6329
AvaI C'yCGr_G
Cuts at: 1085 1271 1374 1650 2750 2888 2990 3349 1085
Size: 186 103 276 1100 138 102 359 4065
Fragments arranged by size:
4065 1100 359 276 186 138 103 102
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 378 411 378
Size: 33 6296
BamHI G'GATC_C
Cuts at: 3240 3354 3240
Size: 114 6215
BanI G'GyrC_C
Cuts at: 898 1612 1662 1747 3288 3345 3759 4007 4042
Size: 714 50 85 1541 57 414 248 35
Cuts at: 4042 898
Size: 3185
Fragments arranged by size:
3185 1541 714 414 248 85 57 50
35
BanII G_rGCy'C
Cuts at: 302 587 913 1381 1490 1856 1932 1964 2000
Size: 285 326 468 109 366 76 32 36
Cuts at: 2000 2223 2991 3050 3343 4373 302
Size: 223 768 59 293 1030 2258
Fragments arranged by size:
2258 1030 768 468 366 326 293 285
223 109 76 59 36 32
BbeI G_GCGC'C
Cuts at: 3763 4011 3763
Size: 248 6081
BbsI GAAGACnn'nnnn_
Cuts at: 650 650
Size: 6329
BbvCI CC'TCA_GC
Cuts at: 374 927 374
Size: 553 5776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 939 973 2602 2636 2741 2775 90
Size: 34 815 34 1629 34 105 34 3644
Fragments arranged by size:
3644 1629 815 105 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3316 4227 4988 6329 3316
Size: 911 761 1341 3316
Fragments arranged by size:
3316 1341 911 761
BclI T'GATC_A
Cuts at: 3849 3849
Size: 6329
BglII A'GATC_T
Cuts at: 1628 3844 5881 1628
Size: 2216 2037 2076
Fragments arranged by size:
2216 2076 2037
BlpI GC'TnA_GC
Cuts at: 586 1715 2383 3441 586
Size: 1129 668 1058 3474
Fragments arranged by size:
3474 1129 1058 668
Bme1580I G_kGCm'C
Cuts at: 1490 1509 1657 1932 2223 2266 2341 2508 2991
Size: 19 148 275 291 43 75 167 483
Cuts at: 2991 3954 4047 5103 1490
Size: 963 93 1056 2716
Fragments arranged by size:
2716 1056 963 483 291 275 167 148
93 75 43 19
BmrI ACTGGGnnnn_n'
Cuts at: 908 939 1511 2502 2556 2775 3541 3622 3957
Size: 31 572 991 54 219 766 81 335
Cuts at: 3957 6101 908
Size: 2144 1136
Fragments arranged by size:
2144 1136 991 766 572 335 219 81
54 31
BmtI G_CTAG'C
Cuts at: 241 241
Size: 6329
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1650 2914 4696 1650
Size: 1264 1782 3283
Fragments arranged by size:
3283 1782 1264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 364 649 1154 1558 1723 1840 1876 2566
Size: 144 285 505 404 165 117 36 690
Cuts at: 2566 2963 3023 3062 3205 3254 220
Size: 397 60 39 143 49 3295
Fragments arranged by size:
3295 690 505 404 397 285 165 144
143 117 60 49 39 36
Bpu10I CC'TnA_GC
Cuts at: 374 907 927 1994 3049 374
Size: 533 20 1067 1055 3654
Fragments arranged by size:
3654 1067 1055 533 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3505 4384 4876 5174 3505
Size: 879 492 298 4660
Fragments arranged by size:
4660 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1892 6033 1892
Size: 4141 2188
BsaAI yAC'GTr
Cuts at: 3213 3682 3213
Size: 469 5860
BsaBI GATnn'nnATC
Cuts at: 6 3843 5880 6
Size: 3837 2037 455
Fragments arranged by size:
3837 2037 455
BsaHI Gr'CG_yC
Cuts at: 263 1497 3760 4008 4710 6206 263
Size: 1234 2263 248 702 1496 386
Fragments arranged by size:
2263 1496 1234 702 386 248
BsaWI w'CCGG_w
Cuts at: 1406 2234 3518 4039 4717 4991 5138 5549 1406
Size: 828 1284 521 678 274 147 411 2186
Fragments arranged by size:
2186 1284 828 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 622 652 1843 1873 1904 1934 2961 2991 622
Size: 30 1191 30 31 30 1027 30 3960
Fragments arranged by size:
3960 1191 1027 31 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 166 186 499 538 1639 1831 1894 2620
Size: 27 20 313 39 1101 192 63 726
Cuts at: 2620 2768 139
Size: 148 3700
Fragments arranged by size:
3700 1101 726 313 192 148 63 39
27 20
BseYI C'CCAG_C
Cuts at: 1244 1252 1385 1743 2068 2072 2216 2983 3156
Size: 8 133 358 325 4 144 767 173
Cuts at: 3156 3221 3754 4512 5089 5585 1244
Size: 65 533 758 577 496 1988
Fragments arranged by size:
1988 767 758 577 533 496 358 325
173 144 133 65 8 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 772 1867 2857 3193 463
Size: 309 1095 990 336 3599
Fragments arranged by size:
3599 1095 990 336 309
BsiEI CG_ry'CG
Cuts at: 104 214 357 3871 3917 5125 104
Size: 110 143 3514 46 1208 1308
Fragments arranged by size:
3514 1308 1208 143 110 46
BsiHKAI G_wGCw'C
Cuts at: 539 587 861 1008 1064 1856 2266 2515 3343
Size: 48 274 147 56 792 410 249 828
Cuts at: 3343 4121 4311 5103 539
Size: 778 190 792 1765
Fragments arranged by size:
1765 828 792 792 778 410 274 249
190 147 56 48
BsiWI C'GTAC_G
Cuts at: 2738 3213 2738
Size: 475 5854
BsmBI CGTCTCn'nnnn_
Cuts at: 3181 3181
Size: 6329
Bsp1286I G_dGCh'C
Cuts at: 302 539 587 861 913 1008 1064 1381 1490
Size: 237 48 274 52 95 56 317 109
Cuts at: 1490 1509 1657 1856 1932 1964 2000 2223 2266
Size: 19 148 199 76 32 36 223 43
Cuts at: 2266 2341 2508 2515 2991 3050 3343 3954 4047
Size: 75 167 7 476 59 293 611 93
Cuts at: 4047 4121 4311 4373 5103 302
Size: 74 190 62 730 1528
Fragments arranged by size:
1528 730 611 476 317 293 274 237
223 199 190 167 148 109 95 93
76 75 74 62 59 56 52 48
43 36 32 19 7
BspEI T'CCGG_A
Cuts at: 5549 5549
Size: 6329
BspHI T'CATG_A
Cuts at: 5505 5505
Size: 6329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 260 930 1600 2915 3215 3379 3593 3895 4276
Size: 670 670 1315 300 164 214 302 381
Cuts at: 4276 260
Size: 2313
Fragments arranged by size:
2313 1315 670 670 381 302 300 214
164
BsrBI CCG'CTC
Cuts at: 50 129 4621 4675 6159 6324 50
Size: 79 4492 54 1484 165 55
Fragments arranged by size:
4492 1484 165 79 55 54
BsrDI GCAATG_nn'
Cuts at: 2696 4241 2696
Size: 1545 4784
BsrFI r'CCGG_y
Cuts at: 3091 4327 4508 4717 3091
Size: 1236 181 209 4703
Fragments arranged by size:
4703 1236 209 181
BssSI C'ACGA_G
Cuts at: 4600 4958 4600
Size: 358 5971
BstAPI GCAn_nnn'nTGC
Cuts at: 1435 1677 2717 2807 3706 1435
Size: 242 1040 90 899 4058
Fragments arranged by size:
4058 1040 899 242 90
BstBI TT'CG_AA
Cuts at: 4690 4690
Size: 6329
BstXI CCAn_nnnn'nTGG
Cuts at: 1132 1260 1461 1132
Size: 128 201 6000
Fragments arranged by size:
6000 201 128
BstYI r'GATC_y
Cuts at: 1 1530 1628 3240 3354 3821 3844 4179 4425
Size: 1529 98 1612 114 467 23 335 246
Cuts at: 4425 5437 5523 5535 5881 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1612 1529 1012 467 449 346 335 246
114 98 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 169 1877 169
Size: 1708 4621
BtgI C'CryG_G
Cuts at: 326 840 937 326
Size: 514 97 5718
Fragments arranged by size:
5718 514 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2286 4279 4460 4623 2286
Size: 1993 181 163 3992
Fragments arranged by size:
3992 1993 181 163
BtsI GCAGTG_nn'
Cuts at: 784 789 1064 1678 2098 3572 784
Size: 5 275 614 420 1474 3541
Fragments arranged by size:
3541 1474 614 420 275 5
DraI TTT'AAA
Cuts at: 3328 3328
Size: 6329
DrdI GACnn_nn'nnGTC
Cuts at: 2956 4035 4893 2956
Size: 1079 858 4392
Fragments arranged by size:
4392 1079 858
EaeI y'GGCC_r
Cuts at: 211 354 2349 2877 3191 3914 4088 4479 4506
Size: 143 1995 528 314 723 174 391 27
Cuts at: 4506 5851 211
Size: 1345 689
Fragments arranged by size:
1995 1345 723 689 528 391 314 174
143 27
EagI C'GGCC_G
Cuts at: 211 354 3914 211
Size: 143 3560 2626
Fragments arranged by size:
3560 2626 143
EarI CTCTTCn'nnn_
Cuts at: 116 2905 3173 3278 4352 4562 116
Size: 2789 268 105 1074 210 1883
Fragments arranged by size:
2789 1883 1074 268 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 859 1957 2275 3333 4537 4847 4993 6139 859
Size: 1098 318 1058 1204 310 146 1146 1049
Fragments arranged by size:
1204 1146 1098 1058 1049 318 310 146
EcoICRI GAG'CTC
Cuts at: 585 1854 3341 585
Size: 1269 1487 3573
Fragments arranged by size:
3573 1487 1269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 364 649 1154 1285 1558 1723 1840 1876
Size: 144 285 505 131 273 165 117 36
Cuts at: 1876 2566 2683 2917 2963 3023 3062 3205 3254
Size: 690 117 234 46 60 39 143 49
Cuts at: 3254 4154 4586 5333 5601 220
Size: 900 432 747 268 948
Fragments arranged by size:
948 900 747 690 505 432 285 273
268 234 165 144 143 131 117 117
60 49 46 39 36
EcoNI CCTnn'n_nnAGG
Cuts at: 1303 3100 1303
Size: 1797 4532
EcoO109I rG'GnC_Cy
Cuts at: 810 888 1120 1471 1487 1734 1929 2220 2244
Size: 78 232 351 16 247 195 291 24
Cuts at: 2244 2484 2682 2987 3468 810
Size: 240 198 305 481 3671
Fragments arranged by size:
3671 481 351 305 291 247 240 232
198 195 78 24 16
EcoRI G'AATT_C
Cuts at: 3333 3333
Size: 6329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3139 4348 3139
Size: 1209 5120
FspI TGC'GCA
Cuts at: 4110 4110
Size: 6329
HaeII r_GCGC'y
Cuts at: 167 1548 1950 1977 2598 3204 3763 4011 5033
Size: 1381 402 27 621 606 559 248 1022
Cuts at: 5033 6012 167
Size: 979 484
Fragments arranged by size:
1381 1022 979 621 606 559 484 402
248 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1843 1870 1875 1902 3290 3322 4171 4201 4203
Size: 27 5 27 1388 32 849 30 2
Cuts at: 4203 4233 1843
Size: 30 3939
Fragments arranged by size:
3939 1388 849 32 30 30 27 27
5 2
HincII GTy'rAC
Cuts at: 950 3368 950
Size: 2418 3911
HindIII A'AGCT_T
Cuts at: 3059 3059
Size: 6329
KasI G'GCGC_C
Cuts at: 3759 4007 3759
Size: 248 6081
KpnI G_GTAC'C
Cuts at: 902 3292 3349 902
Size: 2390 57 3882
Fragments arranged by size:
3882 2390 57
MluI A'CGCG_T
Cuts at: 5762 5762
Size: 6329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 620 2602 5000 5184 620
Size: 1982 2398 184 1765
Fragments arranged by size:
2398 1982 1765 184
MscI TGG'CCA
Cuts at: 4090 4090
Size: 6329
MslI CAynn'nnrTG
Cuts at: 1660 1913 2329 4318 4445 1660
Size: 253 416 1989 127 3544
Fragments arranged by size:
3544 1989 416 253 127
MspA1I CmG'CkG
Cuts at: 258 281 378 636 1204 2072 2544 2804 2983
Size: 23 97 258 568 868 472 260 179
Cuts at: 2983 3082 3440 3754 4114 5127 5372 258
Size: 99 358 314 360 1013 245 1215
Fragments arranged by size:
1215 1013 868 568 472 360 358 314
260 258 245 179 99 97 23
NaeI GCC'GGC
Cuts at: 3093 4510 3093
Size: 1417 4912
NarI GG'CG_CC
Cuts at: 3760 4008 3760
Size: 248 6081
NcoI C'CATG_G
Cuts at: 840 840
Size: 6329
NdeI CA'TA_TG
Cuts at: 1222 1222
Size: 6329
NgoMIV G'CCGG_C
Cuts at: 3091 4508 3091
Size: 1417 4912
NheI G'CTAG_C
Cuts at: 237 237
Size: 6329
NruI TCG'CGA
Cuts at: 2417 2417
Size: 6329
NspI r_CATG'y
Cuts at: 1438 2766 3382 3882 4789 1438
Size: 1328 616 500 907 2978
Fragments arranged by size:
2978 1328 907 616 500
PasI CC'CwG_GG
Cuts at: 966 1738 2679 966
Size: 772 941 4616
Fragments arranged by size:
4616 941 772
PciI A'CATG_T
Cuts at: 4785 4785
Size: 6329
PflMI CCAn_nnn'nTGG
Cuts at: 3150 3228 3150
Size: 78 6251
PfoI T'CCnGG_A
Cuts at: 341 1592 1700 341
Size: 1251 108 4970
Fragments arranged by size:
4970 1251 108
PmeI GTTT'AAAC
Cuts at: 3328 3328
Size: 6329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5468 5500 5468
Size: 32 6297
PpuMI rG'GwC_Cy
Cuts at: 888 1120 1471 1734 2484 888
Size: 232 351 263 750 4733
Fragments arranged by size:
4733 750 351 263 232
PspOMI G'GGCC_C
Cuts at: 1486 1928 2219 2987 1486
Size: 442 291 768 4828
Fragments arranged by size:
4828 768 442 291
PspXI vC'TCGA_Gb
Cuts at: 1374 1374
Size: 6329
PstI C_TGCA'G
Cuts at: 257 671 896 1646 1673 2108 2803 2857 3376
Size: 414 225 750 27 435 695 54 519
Cuts at: 3376 4061 257
Size: 685 2525
Fragments arranged by size:
2525 750 695 685 519 435 414 225
54 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6329
PvuII CAG'CTG
Cuts at: 258 636 1204 2072 2544 2983 3082 3754 4114
Size: 378 568 868 472 439 99 672 360
Cuts at: 4114 258
Size: 2473
Fragments arranged by size:
2473 868 672 568 472 439 378 360
99
RsrII CG'GwC_CG
Cuts at: 4524 4524
Size: 6329
SacI G_AGCT'C
Cuts at: 587 1856 3343 587
Size: 1269 1487 3573
Fragments arranged by size:
3573 1487 1269
SalI G'TCGA_C
Cuts at: 948 3366 948
Size: 2418 3911
SanDI GG'GwC_CC
Cuts at: 1120 1471 2484 1120
Size: 351 1013 4965
Fragments arranged by size:
4965 1013 351
SapI GCTCTTCn'nnn_
Cuts at: 3173 4352 4562 3173
Size: 1179 210 4940
Fragments arranged by size:
4940 1179 210
SbfI CC_TGCA'GG
Cuts at: 896 3376 896
Size: 2480 3849
ScaI AGT'ACT
Cuts at: 566 1648 5846 566
Size: 1082 4198 1049
Fragments arranged by size:
4198 1082 1049
SexAI A'CCwGG_T
Cuts at: 539 539
Size: 6329
SfcI C'TryA_G
Cuts at: 33 253 548 667 892 1642 1669 1942 2104
Size: 220 295 119 225 750 27 273 162
Cuts at: 2104 2799 2853 2944 3372 4057 5050 5241 33
Size: 695 54 91 428 685 993 191 1121
Fragments arranged by size:
1121 993 750 695 685 428 295 273
225 220 191 162 119 91 54 27
SfoI GGC'GCC
Cuts at: 3761 4009 3761
Size: 248 6081
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6329
SmaI CCC'GGG
Cuts at: 2992 3351 2992
Size: 359 5970
SmlI C'TyrA_G
Cuts at: 1374 2888 3484 4399 4891 5153 1374
Size: 1514 596 915 492 262 2550
Fragments arranged by size:
2550 1514 915 596 492 262
SpeI A'CTAG_T
Cuts at: 67 1411 67
Size: 1344 4985
SphI G_CATG'C
Cuts at: 2766 3382 3882 2766
Size: 616 500 5213
Fragments arranged by size:
5213 616 500
SrfI GCCC'GGGC
Cuts at: 2992 2992
Size: 6329
StuI AGG'CCT
Cuts at: 1139 1571 1139
Size: 432 5897
StyI C'CwwG_G
Cuts at: 840 1051 1125 3293 3463 3817 840
Size: 211 74 2168 170 354 3352
Fragments arranged by size:
3352 2168 354 211 170 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 863 1377 1735 2130 2479 3912 4045 4504 4713
Size: 514 358 395 349 1433 133 459 209
Cuts at: 4713 863
Size: 2479
Fragments arranged by size:
2479 1433 514 459 395 358 349 209
133
TatI w'GTAC_w
Cuts at: 564 1646 5844 564
Size: 1082 4198 1049
Fragments arranged by size:
4198 1082 1049
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 260 292 716 735 748 767 781 813 1904
Size: 32 424 19 13 19 14 32 1091
Cuts at: 1904 1936 2256 2288 5846 5878 260
Size: 32 320 32 3558 32 711
Fragments arranged by size:
3558 1091 711 424 320 32 32 32
32 32 19 19 14 13
Tth111I GACn'n_nGTC
Cuts at: 4126 4126
Size: 6329
XbaI T'CTAG_A
Cuts at: 3360 3360
Size: 6329
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 838 880 1260 2515 3229 3824 838
Size: 42 380 1255 714 595 3343
Fragments arranged by size:
3343 1255 714 595 380 42
XhoI C'TCGA_G
Cuts at: 1374 2888 1374
Size: 1514 4815
XmaI C'CCGG_G
Cuts at: 2990 3349 2990
Size: 359 5970
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BfrBI BglI BmgBI
BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NotI NsiI PacI
PmlI PshAI PsiI PsrI SacII SfiI SgrAI SnaBI
SspI SwaI XmnI ZraI