Restriction map for
pF1KB7011
(Circular) (Six-base) MAPSORT of: pF1KB7011.seq Check: 7756 from: 1 to: 6248
pF1KB7011 6248 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 513 513
Size: 6248
AatII G_ACGT'C
Cuts at: 2462 2462
Size: 6248
AccI GT'mk_AC
Cuts at: 1753 3125 3286 1753
Size: 1372 161 4715
Fragments arranged by size:
4715 1372 161
Acc65I G'GTAC_C
Cuts at: 506 3264 506
Size: 2758 3490
AclI AA'CG_TT
Cuts at: 6088 6088
Size: 6248
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 1795 2011 2220 2871 3117 4073 4505 4858
Size: 1598 216 209 651 246 956 432 353
Cuts at: 4858 5520 197
Size: 662 925
Fragments arranged by size:
1598 956 925 662 651 432 353 246
216 209
AfeI AGC'GCT
Cuts at: 931 931
Size: 6248
AflIII A'CryG_T
Cuts at: 556 2846 3095 3152 3212 3600 5400 5681 556
Size: 2290 249 57 60 388 1800 281 1123
Fragments arranged by size:
2290 1800 1123 388 281 249 60 57
AhdI GACnn_n'nnGTC
Cuts at: 1535 2736 2769 1535
Size: 1201 33 5014
Fragments arranged by size:
5014 1201 33
AleI CACnn'nnGTG
Cuts at: 2839 2839
Size: 6248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1940 1940
Size: 6248
AlwNI CAG_nnn'CTG
Cuts at: 246 2193 2243 2325 2961 3553 4991 246
Size: 1947 50 82 636 592 1438 1503
Fragments arranged by size:
1947 1503 1438 636 592 82 50
ApaI G_GGCC'C
Cuts at: 156 156
Size: 6248
ApaLI G'TGCA_C
Cuts at: 2842 5086 2842
Size: 2244 4004
ApoI r'AATT_y
Cuts at: 162 460 700 851 985 1251 1866 1885 2313
Size: 298 240 151 134 266 615 19 428
Cuts at: 2313 2932 3252 5601 5857 162
Size: 619 320 2349 256 553
Fragments arranged by size:
2349 619 615 553 428 320 298 266
256 240 151 134 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 6248
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6248
AvaI C'yCGr_G
Cuts at: 355 1383 2415 3268 355
Size: 1028 1032 853 3335
Fragments arranged by size:
3335 1032 1028 853
BamHI G'GATC_C
Cuts at: 3273 3273
Size: 6248
BanI G'GyrC_C
Cuts at: 506 1306 1443 2568 2855 3264 3678 3926 3961
Size: 800 137 1125 287 409 414 248 35
Cuts at: 3961 506
Size: 2793
Fragments arranged by size:
2793 1125 800 414 409 287 248 137
35
BanII G_rGCy'C
Cuts at: 156 288 1073 1390 1618 2080 2429 3262 4292
Size: 132 785 317 228 462 349 833 1030
Cuts at: 4292 156
Size: 2112
Fragments arranged by size:
2112 1030 833 785 462 349 317 228
132
BbeI G_GCGC'C
Cuts at: 1447 3682 3930 1447
Size: 2235 248 3765
Fragments arranged by size:
3765 2235 248
BbsI GAAGACnn'nnnn_
Cuts at: 2561 2561
Size: 6248
BbvCI CC'TCA_GC
Cuts at: 668 1499 2404 2926 668
Size: 831 905 522 3990
Fragments arranged by size:
3990 905 831 522
BciVI GTATCCnnnnn_n'
Cuts at: 969 4146 5202 6248 969
Size: 3177 1056 1046 969
Fragments arranged by size:
3177 1056 1046 969
BclI T'GATC_A
Cuts at: 366 1643 2300 3768 366
Size: 1277 657 1468 2846
Fragments arranged by size:
2846 1468 1277 657
BglI GCCn_nnn'nGGC
Cuts at: 1441 2053 2257 2758 1441
Size: 612 204 501 4931
Fragments arranged by size:
4931 612 501 204
BglII A'GATC_T
Cuts at: 3763 5800 3763
Size: 2037 4211
BlpI GC'TnA_GC
Cuts at: 913 3360 913
Size: 2447 3801
Bme1580I G_kGCm'C
Cuts at: 156 2573 2846 3873 3966 5090 156
Size: 2417 273 1027 93 1124 1314
Fragments arranged by size:
2417 1314 1124 1027 273 93
BmgBI CAC'GTC
Cuts at: 557 3123 557
Size: 2566 3682
BmrI ACTGGGnnnn_n'
Cuts at: 157 903 3460 3541 3876 6020 157
Size: 746 2557 81 335 2144 385
Fragments arranged by size:
2557 2144 746 385 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1514 4615 1514
Size: 3101 3147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 1301 2080 2110 2215 2413 2827 1285
Size: 16 779 30 105 198 414 4706
Fragments arranged by size:
4706 779 414 198 105 30 16
Bpu10I CC'TnA_GC
Cuts at: 668 1499 1612 1742 2404 2785 2926 668
Size: 831 113 130 662 381 141 3990
Fragments arranged by size:
3990 831 662 381 141 130 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 1888 2320 3424 4303 5017 5315 527
Size: 1361 432 1104 879 714 298 1460
Fragments arranged by size:
1460 1361 1104 879 714 432 298
BsaI GGTCTCn'nnnn_
Cuts at: 5952 5952
Size: 6248
BsaAI yAC'GTr
Cuts at: 2847 3096 3213 3601 2847
Size: 249 117 388 5494
Fragments arranged by size:
5494 388 249 117
BsaBI GATnn'nnATC
Cuts at: 6 3762 5799 6
Size: 3756 2037 455
Fragments arranged by size:
3756 2037 455
BsaHI Gr'CG_yC
Cuts at: 1444 1829 2459 3679 3927 4629 6125 1444
Size: 385 630 1220 248 702 1496 1567
Fragments arranged by size:
1567 1496 1220 702 630 385 248
BsaWI w'CCGG_w
Cuts at: 788 1220 3437 3958 4636 5047 5194 5468 788
Size: 432 2217 521 678 411 147 274 1568
Fragments arranged by size:
2217 1568 678 521 432 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1360 1390 1587 1617 2188 2218 2893 2923 1360
Size: 30 197 30 571 30 675 30 4685
Fragments arranged by size:
4685 675 571 197 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1413 1474 1477 2560 1413
Size: 61 3 1083 5101
Fragments arranged by size:
5101 1083 61 3
BseYI C'CCAG_C
Cuts at: 1484 3673 4431 5096 5504 1484
Size: 2189 758 665 408 2228
Fragments arranged by size:
2228 2189 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 2149 2311 3091 1204
Size: 945 162 780 4361
Fragments arranged by size:
4361 945 780 162
BsiEI CG_ry'CG
Cuts at: 104 3790 3836 5066 104
Size: 3686 46 1230 1286
Fragments arranged by size:
3686 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 288 644 1285 1813 2080 2846 3262 4040 4230
Size: 356 641 528 267 766 416 778 190
Cuts at: 4230 5090 288
Size: 860 1446
Fragments arranged by size:
1446 860 778 766 641 528 416 356
267 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2877 2877
Size: 6248
Bsp1286I G_dGCh'C
Cuts at: 156 288 644 1073 1285 1390 1618 1813 2080
Size: 132 356 429 212 105 228 195 267
Cuts at: 2080 2429 2573 2846 3262 3873 3966 4040 4230
Size: 349 144 273 416 611 93 74 190
Cuts at: 4230 4292 5090 156
Size: 62 798 1314
Fragments arranged by size:
1314 798 611 429 416 356 349 273
267 228 212 195 190 144 132 105
93 74 62
BspEI T'CCGG_A
Cuts at: 1220 1220
Size: 6248
BspHI T'CATG_A
Cuts at: 4680 4680
Size: 6248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 513 539 1134 2930 3298 3512 3814 4195 513
Size: 26 595 1796 368 214 302 381 2566
Fragments arranged by size:
2566 1796 595 381 368 302 214 26
BsrBI CCG'CTC
Cuts at: 50 1943 2139 2199 2526 4540 4594 6078 6243
Size: 1893 196 60 327 2014 54 1484 165
Cuts at: 6243 50
Size: 55
Fragments arranged by size:
2014 1893 1484 327 196 165 60 55
54
BsrDI GCAATG_nn'
Cuts at: 4160 4160
Size: 6248
BsrFI r'CCGG_y
Cuts at: 1334 2168 4246 4427 1334
Size: 834 2078 181 3155
Fragments arranged by size:
3155 2078 834 181
BssSI C'ACGA_G
Cuts at: 223 331 2472 4519 5227 223
Size: 108 2141 2047 708 1244
Fragments arranged by size:
2141 2047 1244 708 108
BstAPI GCAn_nnn'nTGC
Cuts at: 3625 3625
Size: 6248
BstBI TT'CG_AA
Cuts at: 3158 4609 3158
Size: 1451 4797
BstXI CCAn_nnnn'nTGG
Cuts at: 2613 2676 2613
Size: 63 6185
BstYI r'GATC_y
Cuts at: 1 393 1779 3273 3740 3763 4098 4344 4650
Size: 392 1386 1494 467 23 335 246 306
Cuts at: 4650 4662 4748 5800 1
Size: 12 86 1052 449
Fragments arranged by size:
1494 1386 1052 467 449 392 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1409 1448 2289 2511 2756 2826 2876 107
Size: 1302 39 841 222 245 70 50 3479
Fragments arranged by size:
3479 1302 841 245 222 70 50 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1994 2786 2877 4198 4379 4542 1994
Size: 792 91 1321 181 163 3700
Fragments arranged by size:
3700 1321 792 181 163 91
BtsI GCAGTG_nn'
Cuts at: 2161 2574 2903 3189 3491 2161
Size: 413 329 286 302 4918
Fragments arranged by size:
4918 413 329 302 286
DraI TTT'AAA
Cuts at: 2505 3247 2505
Size: 742 5506
DraIII CAC_nnn'GTG
Cuts at: 386 597 1799 386
Size: 211 1202 4835
Fragments arranged by size:
4835 1202 211
DrdI GACnn_nn'nnGTC
Cuts at: 3954 5298 3954
Size: 1344 4904
EaeI y'GGCC_r
Cuts at: 800 833 949 995 2158 2615 2666 2714 3833
Size: 33 116 46 1163 457 51 48 1119
Cuts at: 3833 4007 4398 4425 5770 800
Size: 174 391 27 1345 1278
Fragments arranged by size:
1345 1278 1163 1119 457 391 174 116
51 48 46 33 27
EagI C'GGCC_G
Cuts at: 3833 3833
Size: 6248
EarI CTCTTCn'nnn_
Cuts at: 1061 1451 1702 2680 4271 4481 1061
Size: 390 251 978 1591 210 2828
Fragments arranged by size:
2828 1591 978 390 251 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 458 1350 1441 2695 3154 4456 5198 5344 6058
Size: 892 91 1254 459 1302 742 146 714
Cuts at: 6058 458
Size: 648
Fragments arranged by size:
1302 1254 892 742 714 648 459 146
91
EcoICRI GAG'CTC
Cuts at: 286 2078 3260 286
Size: 1792 1182 3274
Fragments arranged by size:
3274 1792 1182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 1285 1301 1795 2011 2080 2110 2215 2220
Size: 1088 16 494 216 69 30 105 5
Cuts at: 2220 2413 2827 2871 3117 4073 4505 4858 5520
Size: 193 414 44 246 956 432 353 662
Cuts at: 5520 197
Size: 925
Fragments arranged by size:
1088 956 925 662 494 432 414 353
246 216 193 105 69 44 30 16
5
EcoO109I rG'GnC_Cy
Cuts at: 152 153 1481 2055 2190 2817 3387 152
Size: 1 1328 574 135 627 570 3013
Fragments arranged by size:
3013 1328 627 574 570 135 1
EcoRI G'AATT_C
Cuts at: 851 985 1251 3252 851
Size: 134 266 2001 3847
Fragments arranged by size:
3847 2001 266 134
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 552 933 3126 3250 4267 552
Size: 381 2193 124 1017 2533
Fragments arranged by size:
2533 2193 1017 381 124
FspI TGC'GCA
Cuts at: 4029 4029
Size: 6248
HaeII r_GCGC'y
Cuts at: 933 1447 2792 2814 3682 3930 5160 5931 933
Size: 514 1345 22 868 248 1230 771 1250
Fragments arranged by size:
1345 1250 1230 868 771 514 248 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 750 782 984 1016 1360 1392 1521 1553 1587
Size: 32 202 32 344 32 129 32 34
Cuts at: 1587 1619 2722 2754 2755 2787 4090 4120 4122
Size: 32 1103 32 1 32 1303 30 2
Cuts at: 4122 4152 750
Size: 30 2846
Fragments arranged by size:
2846 1303 1103 344 202 129 34 32
32 32 32 32 32 32 30 30
2 1
HincII GTy'rAC
Cuts at: 554 3287 554
Size: 2733 3515
KasI G'GCGC_C
Cuts at: 1443 3678 3926 1443
Size: 2235 248 3765
Fragments arranged by size:
3765 2235 248
KpnI G_GTAC'C
Cuts at: 510 3268 510
Size: 2758 3490
MluI A'CGCG_T
Cuts at: 5681 5681
Size: 6248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1111 5007 5191 1111
Size: 3896 184 2168
Fragments arranged by size:
3896 2168 184
MscI TGG'CCA
Cuts at: 997 2668 2716 4009 997
Size: 1671 48 1293 3236
Fragments arranged by size:
3236 1671 1293 48
MslI CAynn'nnrTG
Cuts at: 2272 2611 2662 2839 2896 4237 4364 2272
Size: 339 51 177 57 1341 127 4156
Fragments arranged by size:
4156 1341 339 177 127 57 51
MspA1I CmG'CkG
Cuts at: 1411 1653 2598 2758 2828 3359 3673 4033 4817
Size: 242 945 160 70 531 314 360 784
Cuts at: 4817 5062 1411
Size: 245 2597
Fragments arranged by size:
2597 945 784 531 360 314 245 242
160 70
NaeI GCC'GGC
Cuts at: 1336 4429 1336
Size: 3093 3155
NarI GG'CG_CC
Cuts at: 1444 3679 3927 1444
Size: 2235 248 3765
Fragments arranged by size:
3765 2235 248
NcoI C'CATG_G
Cuts at: 107 2876 107
Size: 2769 3479
NgoMIV G'CCGG_C
Cuts at: 1334 4427 1334
Size: 3093 3155
NruI TCG'CGA
Cuts at: 3204 3204
Size: 6248
NspI r_CATG'y
Cuts at: 2442 3301 3801 5404 2442
Size: 859 500 1603 3286
Fragments arranged by size:
3286 1603 859 500
PciI A'CATG_T
Cuts at: 5400 5400
Size: 6248
PflMI CCAn_nnn'nTGG
Cuts at: 246 2193 246
Size: 1947 4301
PfoI T'CCnGG_A
Cuts at: 2192 2373 2192
Size: 181 6067
PmeI GTTT'AAAC
Cuts at: 3247 3247
Size: 6248
PmlI CAC'GTG
Cuts at: 2847 3213 2847
Size: 366 5882
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3139 3171 4694 4726 3139
Size: 32 1523 32 4661
Fragments arranged by size:
4661 1523 32 32
PpuMI rG'GwC_Cy
Cuts at: 1481 2190 1481
Size: 709 5539
PspOMI G'GGCC_C
Cuts at: 152 152
Size: 6248
PspXI vC'TCGA_Gb
Cuts at: 355 355
Size: 6248
PstI C_TGCA'G
Cuts at: 524 2104 2120 3295 3980 524
Size: 1580 16 1175 685 2792
Fragments arranged by size:
2792 1580 1175 685 16
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6248
PvuII CAG'CTG
Cuts at: 3673 4033 3673
Size: 360 5888
RsrII CG'GwC_CG
Cuts at: 2630 4443 2630
Size: 1813 4435
SacI G_AGCT'C
Cuts at: 288 2080 3262 288
Size: 1792 1182 3274
Fragments arranged by size:
3274 1792 1182
SacII CC_GC'GG
Cuts at: 1412 2759 2829 1412
Size: 1347 70 4831
Fragments arranged by size:
4831 1347 70
SalI G'TCGA_C
Cuts at: 3285 3285
Size: 6248
SapI GCTCTTCn'nnn_
Cuts at: 1061 2680 4271 4481 1061
Size: 1619 1591 210 2828
Fragments arranged by size:
2828 1619 1591 210
SbfI CC_TGCA'GG
Cuts at: 3295 3295
Size: 6248
ScaI AGT'ACT
Cuts at: 5765 5765
Size: 6248
SexAI A'CCwGG_T
Cuts at: 2186 2186
Size: 6248
SfcI C'TryA_G
Cuts at: 33 520 1241 2100 2116 3291 3976 4944 5135
Size: 487 721 859 16 1175 685 968 191
Cuts at: 5135 33
Size: 1146
Fragments arranged by size:
1175 1146 968 859 721 685 487 191
16
SfiI GGCCn_nnn'nGGCC
Cuts at: 2053 2053
Size: 6248
SfoI GGC'GCC
Cuts at: 1445 3680 3928 1445
Size: 2235 248 3765
Fragments arranged by size:
3765 2235 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6248
SmaI CCC'GGG
Cuts at: 3270 3270
Size: 6248
SmlI C'TyrA_G
Cuts at: 355 542 1903 2335 3403 4318 5032 5294 355
Size: 187 1361 432 1068 915 714 262 1309
Fragments arranged by size:
1361 1309 1068 915 714 432 262 187
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6248
SphI G_CATG'C
Cuts at: 3301 3801 3301
Size: 500 5748
StuI AGG'CCT
Cuts at: 1633 1669 2047 1633
Size: 36 378 5834
Fragments arranged by size:
5834 378 36
StyI C'CwwG_G
Cuts at: 107 179 629 803 899 980 2048 2876 3382
Size: 72 450 174 96 81 1068 828 506
Cuts at: 3382 3736 107
Size: 354 2619
Fragments arranged by size:
2619 1068 828 506 450 354 174 96
81 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 271 2618 2955 3831 3964 4423 4632 271
Size: 2347 337 876 133 459 209 1887
Fragments arranged by size:
2347 1887 876 459 337 209 133
TatI w'GTAC_w
Cuts at: 3149 5763 3149
Size: 2614 3634
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 209 241 842 874 1017 1049 2188 2220 3100
Size: 32 601 32 143 32 1139 32 880
Cuts at: 3100 3132 5765 5797 209
Size: 32 2633 32 660
Fragments arranged by size:
2633 1139 880 660 601 143 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1599 4045 1599
Size: 2446 3802
XbaI T'CTAG_A
Cuts at: 3279 3279
Size: 6248
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 715 2874 3743 715
Size: 2159 869 3220
Fragments arranged by size:
3220 2159 869
XhoI C'TCGA_G
Cuts at: 355 355
Size: 6248
XmaI C'CCGG_G
Cuts at: 3268 3268
Size: 6248
XmnI GAAnn'nnTTC
Cuts at: 1711 1711
Size: 6248
ZraI GAC'GTC
Cuts at: 2460 2460
Size: 6248
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AloI AscI AvrII BaeI BcgI BfrBI
BmtI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PasI PshAI
PsiI PsrI SanDI SgrAI SnaBI SrfI SspI SwaI