Restriction map for
pF1KB7013
(Circular) (Six-base) MAPSORT of: pF1KB7013.seq Check: 9508 from: 1 to: 6371
pF1KB7013 6371 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 424 1813 3207 424
Size: 1389 1394 3588
Fragments arranged by size:
3588 1394 1389
AccI GT'mk_AC
Cuts at: 183 1040 3409 183
Size: 857 2369 3145
Fragments arranged by size:
3145 2369 857
Acc65I G'GTAC_C
Cuts at: 2158 3387 2158
Size: 1229 5142
AclI AA'CG_TT
Cuts at: 6211 6211
Size: 6371
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 1497 4196 4628 5375 5643 1315
Size: 182 2699 432 747 268 2043
Fragments arranged by size:
2699 2043 747 432 268 182
AflIII A'CryG_T
Cuts at: 186 1856 3322 3723 4827 5804 186
Size: 1670 1466 401 1104 977 753
Fragments arranged by size:
1670 1466 1104 977 753 401
AgeI A'CCGG_T
Cuts at: 4759 4759
Size: 6371
AlwNI CAG_nnn'CTG
Cuts at: 772 1474 2542 3106 3676 5243 772
Size: 702 1068 564 570 1567 1900
Fragments arranged by size:
1900 1567 1068 702 570 564
ApaI G_GGCC'C
Cuts at: 2116 2643 2116
Size: 527 5844
ApaLI G'TGCA_C
Cuts at: 2693 5141 2693
Size: 2448 3923
ApoI r'AATT_y
Cuts at: 260 2819 2990 3375 5724 5980 260
Size: 2559 171 385 2349 256 651
Fragments arranged by size:
2559 2349 651 385 256 171
AseI AT'TA_AT
Cuts at: 20 20
Size: 6371
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6371
AvaI C'yCGr_G
Cuts at: 1986 3391 1986
Size: 1405 4966
AvrII C'CTAG_G
Cuts at: 1980 1980
Size: 6371
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1741 1774 1741
Size: 33 6338
BamHI G'GATC_C
Cuts at: 3396 3396
Size: 6371
BanI G'GyrC_C
Cuts at: 436 1447 1915 2005 2158 2785 3387 3801 4049
Size: 1011 468 90 153 627 602 414 248
Cuts at: 4049 4084 436
Size: 35 2723
Fragments arranged by size:
2723 1011 627 602 468 414 248 153
90 35
BanII G_rGCy'C
Cuts at: 498 926 2116 2643 3385 4415 498
Size: 428 1190 527 742 1030 2454
Fragments arranged by size:
2454 1190 1030 742 527 428
BbeI G_GCGC'C
Cuts at: 3805 4053 3805
Size: 248 6123
BbsI GAAGACnn'nnnn_
Cuts at: 1471 1525 1471
Size: 54 6317
BbvCI CC'TCA_GC
Cuts at: 1151 3041 1151
Size: 1890 4481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 830 864 830
Size: 34 6337
BciVI GTATCCnnnnn_n'
Cuts at: 1556 1769 4269 5030 6371 1556
Size: 213 2500 761 1341 1556
Fragments arranged by size:
2500 1556 1341 761 213
BclI T'GATC_A
Cuts at: 417 3891 417
Size: 3474 2897
BglI GCCn_nnn'nGGC
Cuts at: 1255 2436 1255
Size: 1181 5190
BglII A'GATC_T
Cuts at: 3886 5923 3886
Size: 2037 4334
BlpI GC'TnA_GC
Cuts at: 682 920 1475 3483 682
Size: 238 555 2008 3570
Fragments arranged by size:
3570 2008 555 238
Bme1580I G_kGCm'C
Cuts at: 441 1452 2116 2643 2697 3996 4089 5145 441
Size: 1011 664 527 54 1299 93 1056 1667
Fragments arranged by size:
1667 1299 1056 1011 664 527 93 54
BmgBI CAC'GTC
Cuts at: 2041 2041
Size: 6371
BmrI ACTGGGnnnn_n'
Cuts at: 1447 1576 2065 2992 3583 3664 3999 6143 1447
Size: 129 489 927 591 81 335 2144 1675
Fragments arranged by size:
2144 1675 927 591 489 335 129 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4738 4738
Size: 6371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 770 1067 2096 2159 3319 770
Size: 297 1029 63 1160 3822
Fragments arranged by size:
3822 1160 1029 297 63
Bpu10I CC'TnA_GC
Cuts at: 665 1151 3041 665
Size: 486 1890 3995
Fragments arranged by size:
3995 1890 486
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1427 3140 3217 3547 4426 4918 5216 1427
Size: 1713 77 330 879 492 298 2582
Fragments arranged by size:
2582 1713 879 492 330 298 77
BsaI GGTCTCn'nnnn_
Cuts at: 1558 2378 2407 2914 6075 1558
Size: 820 29 507 3161 1854
Fragments arranged by size:
3161 1854 820 507 29
BsaAI yAC'GTr
Cuts at: 187 3724 187
Size: 3537 2834
BsaBI GATnn'nnATC
Cuts at: 6 3885 5922 6
Size: 3879 2037 455
Fragments arranged by size:
3879 2037 455
BsaHI Gr'CG_yC
Cuts at: 2357 3802 4050 4752 6248 2357
Size: 1445 248 702 1496 2480
Fragments arranged by size:
2480 1496 1445 702 248
BsaWI w'CCGG_w
Cuts at: 3560 4081 4759 5033 5180 5591 3560
Size: 521 678 274 147 411 4340
Fragments arranged by size:
4340 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 576 820 1237 1416 2636 3796 4554 5131 5627
Size: 244 417 179 1220 1160 758 577 496
Cuts at: 5627 576
Size: 1320
Fragments arranged by size:
1320 1220 1160 758 577 496 417 244
179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2669 2936 2669
Size: 267 6104
BsiEI CG_ry'CG
Cuts at: 104 3913 3959 5167 104
Size: 3809 46 1208 1308
Fragments arranged by size:
3809 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2697 3385 4163 4353 5145 2697
Size: 688 778 190 792 3923
Fragments arranged by size:
3923 792 778 688 190
BsmI GAATG_Cn'
Cuts at: 1798 1853 3017 1798
Size: 55 1164 5152
Fragments arranged by size:
5152 1164 55
Bsp1286I G_dGCh'C
Cuts at: 441 498 926 1452 2116 2643 2697 3385 3996
Size: 57 428 526 664 527 54 688 611
Cuts at: 3996 4089 4163 4353 4415 5145 441
Size: 93 74 190 62 730 1667
Fragments arranged by size:
1667 730 688 664 611 527 526 428
190 93 74 62 57 54
BspEI T'CCGG_A
Cuts at: 5591 5591
Size: 6371
BspHI T'CATG_A
Cuts at: 525 5547 525
Size: 5022 1349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 424 1241 1540 1813 1910 3207 3421 3635 3937
Size: 817 299 273 97 1297 214 214 302
Cuts at: 3937 4318 424
Size: 381 2477
Fragments arranged by size:
2477 1297 817 381 302 299 273 214
214 97
BsrBI CCG'CTC
Cuts at: 50 4663 4717 6201 6366 50
Size: 4613 54 1484 165 55
Fragments arranged by size:
4613 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 641 698 1577 1817 2055 2378 2750 2936 4283
Size: 57 879 240 238 323 372 186 1347
Cuts at: 4283 641
Size: 2729
Fragments arranged by size:
2729 1347 879 372 323 240 238 186
57
BsrFI r'CCGG_y
Cuts at: 1912 4369 4550 4759 1912
Size: 2457 181 209 3524
Fragments arranged by size:
3524 2457 209 181
BssSI C'ACGA_G
Cuts at: 144 4642 5000 144
Size: 4498 358 1515
Fragments arranged by size:
4498 1515 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3748 3748
Size: 6371
BstBI TT'CG_AA
Cuts at: 4732 4732
Size: 6371
BstEII G'GTnAC_C
Cuts at: 1611 1611
Size: 6371
BstXI CCAn_nnnn'nTGG
Cuts at: 1245 2328 1245
Size: 1083 5288
BstYI r'GATC_y
Cuts at: 1 3162 3396 3863 3886 4221 4467 5479 5565
Size: 3161 234 467 23 335 246 1012 86
Cuts at: 5565 5577 5923 1
Size: 12 346 449
Fragments arranged by size:
3161 1012 467 449 346 335 246 234
86 23 12
BtgI C'CryG_G
Cuts at: 107 689 2813 107
Size: 582 2124 3665
Fragments arranged by size:
3665 2124 582
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2158 4321 4502 4665 2158
Size: 2163 181 163 3864
Fragments arranged by size:
3864 2163 181 163
BtsI GCAGTG_nn'
Cuts at: 3266 3614 3266
Size: 348 6023
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 214 249 214
Size: 35 6336
DraI TTT'AAA
Cuts at: 542 3370 542
Size: 2828 3543
DrdI GACnn_nn'nnGTC
Cuts at: 2602 4077 4935 2602
Size: 1475 858 4038
Fragments arranged by size:
4038 1475 858
EaeI y'GGCC_r
Cuts at: 692 1247 3293 3956 4130 4521 4548 5893 692
Size: 555 2046 663 174 391 27 1345 1170
Fragments arranged by size:
2046 1345 1170 663 555 391 174 27
EagI C'GGCC_G
Cuts at: 3956 3956
Size: 6371
EarI CTCTTCn'nnn_
Cuts at: 3226 4394 4604 3226
Size: 1168 210 4993
Fragments arranged by size:
4993 1168 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4579 4889 5035 6181 4579
Size: 310 146 1146 4769
Fragments arranged by size:
4769 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3383 3383
Size: 6371
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 770 1067 1315 1497 2096 2159 3319 4196 4628
Size: 297 248 182 599 63 1160 877 432
Cuts at: 4628 5375 5643 770
Size: 747 268 1498
Fragments arranged by size:
1498 1160 877 747 599 432 297 268
248 182 63
EcoO109I rG'GnC_Cy
Cuts at: 170 1779 2640 3510 170
Size: 1609 861 870 3031
Fragments arranged by size:
3031 1609 870 861
EcoRI G'AATT_C
Cuts at: 260 2990 3375 260
Size: 2730 385 3256
Fragments arranged by size:
3256 2730 385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 553 4390 553
Size: 3837 2534
FspI TGC'GCA
Cuts at: 4152 4152
Size: 6371
HaeII r_GCGC'y
Cuts at: 247 3805 4053 5075 6054 247
Size: 3558 248 1022 979 564
Fragments arranged by size:
3558 1022 979 564 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1656 1688 3154 3186 4213 4243 4245 4275 1656
Size: 32 1466 32 1027 30 2 30 3752
Fragments arranged by size:
3752 1466 1027 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1529 1815 3410 1529
Size: 286 1595 4490
Fragments arranged by size:
4490 1595 286
HindIII A'AGCT_T
Cuts at: 398 2505 398
Size: 2107 4264
KasI G'GCGC_C
Cuts at: 3801 4049 3801
Size: 248 6123
KpnI G_GTAC'C
Cuts at: 2162 3391 2162
Size: 1229 5142
MfeI C'AATT_G
Cuts at: 659 860 2174 2858 659
Size: 201 1314 684 4172
Fragments arranged by size:
4172 1314 684 201
MluI A'CGCG_T
Cuts at: 5804 5804
Size: 6371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 264 535 1298 3050 5042 5226 264
Size: 271 763 1752 1992 184 1409
Fragments arranged by size:
1992 1752 1409 763 271 184
MscI TGG'CCA
Cuts at: 694 1249 4132 694
Size: 555 2883 2933
Fragments arranged by size:
2933 2883 555
MslI CAynn'nnrTG
Cuts at: 2563 2737 3295 4360 4487 2563
Size: 174 558 1065 127 4447
Fragments arranged by size:
4447 1065 558 174 127
MspA1I CmG'CkG
Cuts at: 769 2248 2578 2800 3482 3796 4156 5169 5414
Size: 1479 330 222 682 314 360 1013 245
Cuts at: 5414 769
Size: 1726
Fragments arranged by size:
1726 1479 1013 682 360 330 314 245
222
NaeI GCC'GGC
Cuts at: 1914 4552 1914
Size: 2638 3733
NarI GG'CG_CC
Cuts at: 3802 4050 3802
Size: 248 6123
NcoI C'CATG_G
Cuts at: 107 689 2813 107
Size: 582 2124 3665
Fragments arranged by size:
3665 2124 582
NdeI CA'TA_TG
Cuts at: 3057 3057
Size: 6371
NgoMIV G'CCGG_C
Cuts at: 1912 4550 1912
Size: 2638 3733
NspI r_CATG'y
Cuts at: 1860 2601 3189 3326 3424 3924 4831 1860
Size: 741 588 137 98 500 907 3400
Fragments arranged by size:
3400 907 741 588 500 137 98
PasI CC'CwG_GG
Cuts at: 2028 2028
Size: 6371
PciI A'CATG_T
Cuts at: 1856 3322 4827 1856
Size: 1466 1505 3400
Fragments arranged by size:
3400 1505 1466
PflMI CCAn_nnn'nTGG
Cuts at: 2905 2905
Size: 6371
PmeI GTTT'AAAC
Cuts at: 3370 3370
Size: 6371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2646 5510 5542 2614
Size: 32 2864 32 3443
Fragments arranged by size:
3443 2864 32 32
PpuMI rG'GwC_Cy
Cuts at: 170 170
Size: 6371
PsiI TTA'TAA
Cuts at: 710 1958 710
Size: 1248 5123
PspOMI G'GGCC_C
Cuts at: 2112 2639 2112
Size: 527 5844
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 201 233 201
Size: 32 6339
PstI C_TGCA'G
Cuts at: 837 891 1725 3261 3418 4103 837
Size: 54 834 1536 157 685 3105
Fragments arranged by size:
3105 1536 834 685 157 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6371
PvuII CAG'CTG
Cuts at: 769 2248 2578 2800 3796 4156 769
Size: 1479 330 222 996 360 2984
Fragments arranged by size:
2984 1479 996 360 330 222
RsrII CG'GwC_CG
Cuts at: 4566 4566
Size: 6371
SacI G_AGCT'C
Cuts at: 3385 3385
Size: 6371
SalI G'TCGA_C
Cuts at: 3408 3408
Size: 6371
SapI GCTCTTCn'nnn_
Cuts at: 4394 4604 4394
Size: 210 6161
SbfI CC_TGCA'GG
Cuts at: 3418 3418
Size: 6371
ScaI AGT'ACT
Cuts at: 5888 5888
Size: 6371
SexAI A'CCwGG_T
Cuts at: 641 641
Size: 6371
SfcI C'TryA_G
Cuts at: 33 423 623 833 887 1385 1721 1947 3257
Size: 390 200 210 54 498 336 226 1310
Cuts at: 3257 3414 4099 5092 5283 33
Size: 157 685 993 191 1121
Fragments arranged by size:
1310 1121 993 685 498 390 336 226
210 200 191 157 54
SfiI GGCCn_nnn'nGGCC
Cuts at: 1255 1255
Size: 6371
SfoI GGC'GCC
Cuts at: 3803 4051 3803
Size: 248 6123
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6371
SmaI CCC'GGG
Cuts at: 3393 3393
Size: 6371
SmlI C'TyrA_G
Cuts at: 1442 3155 3196 3526 4441 4933 5195 1442
Size: 1713 41 330 915 492 262 2618
Fragments arranged by size:
2618 1713 915 492 330 262 41
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6371
SphI G_CATG'C
Cuts at: 3189 3424 3924 3189
Size: 235 500 5636
Fragments arranged by size:
5636 500 235
SspI AAT'ATT
Cuts at: 1437 1437
Size: 6371
StyI C'CwwG_G
Cuts at: 107 447 689 926 1980 2009 2813 3505 3859
Size: 340 242 237 1054 29 804 692 354
Cuts at: 3859 107
Size: 2619
Fragments arranged by size:
2619 1054 804 692 354 340 242 237
29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 844 2041 2143 2551 2680 3011 3232 3954 4087
Size: 1197 102 408 129 331 221 722 133
Cuts at: 4087 4546 4755 844
Size: 459 209 2460
Fragments arranged by size:
2460 1197 722 459 408 331 221 209
133 129 102
TatI w'GTAC_w
Cuts at: 2250 2270 5886 2250
Size: 20 3616 2735
Fragments arranged by size:
3616 2735 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1373 1405 5888 5920 1373
Size: 32 4483 32 1824
Fragments arranged by size:
4483 1824 32 32
Tth111I GACn'n_nGTC
Cuts at: 1524 4168 1524
Size: 2644 3727
XbaI T'CTAG_A
Cuts at: 3402 3402
Size: 6371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1623 2943 3866 1623
Size: 1320 923 4128
Fragments arranged by size:
4128 1320 923
XmaI C'CCGG_G
Cuts at: 3391 3391
Size: 6371
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
BfrBI BmtI BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HpaI NheI NotI NruI NsiI PacI PfoI PmlI
PshAI PspXI SacII SanDI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI