Restriction map for
pF1KB7014
(Circular) (Six-base) MAPSORT of: pF1KB7014.seq Check: 9028 from: 1 to: 8855
pF1KB7014 8855 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 177 1668 2712 3705 3820 3852 5811 177
Size: 1491 1044 993 115 32 1959 3221
Fragments arranged by size:
3221 1959 1491 1044 993 115 32
AatII G_ACGT'C
Cuts at: 4880 4978 4880
Size: 98 8757
AccI GT'mk_AC
Cuts at: 929 5893 929
Size: 4964 3891
Acc65I G'GTAC_C
Cuts at: 5871 5871
Size: 8855
AclI AA'CG_TT
Cuts at: 8695 8695
Size: 8855
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3670 5250 6680 7112 7859 8127 3670
Size: 1580 1430 432 747 268 4398
Fragments arranged by size:
4398 1580 1430 747 432 268
AfeI AGC'GCT
Cuts at: 1825 3025 3231 1825
Size: 1200 206 7449
Fragments arranged by size:
7449 1200 206
AflIII A'CryG_T
Cuts at: 803 1715 2213 2264 5312 6207 7311 8288 803
Size: 912 498 51 3048 895 1104 977 1370
Fragments arranged by size:
3048 1370 1104 977 912 895 498 51
AgeI A'CCGG_T
Cuts at: 7243 7243
Size: 8855
AhdI GACnn_n'nnGTC
Cuts at: 867 867
Size: 8855
AleI CACnn'nnGTG
Cuts at: 3034 4378 3034
Size: 1344 7511
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4879 5410 4879
Size: 531 8324
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1156 1188 3386 3418 4528 4560 1156
Size: 32 2198 32 1110 32 5451
Fragments arranged by size:
5451 2198 1110 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 305 582 1404 1781 2289 2741 4245 5802 6160
Size: 277 822 377 508 452 1504 1557 358
Cuts at: 6160 7727 305
Size: 1567 1433
Fragments arranged by size:
1567 1557 1504 1433 822 508 452 377
358 277
ApaI G_GGCC'C
Cuts at: 411 424 2785 4588 411
Size: 13 2361 1803 4678
Fragments arranged by size:
4678 2361 1803 13
ApaLI G'TGCA_C
Cuts at: 2217 7625 2217
Size: 5408 3447
ApoI r'AATT_y
Cuts at: 1033 2466 5859 8208 8464 1033
Size: 1433 3393 2349 256 1424
Fragments arranged by size:
3393 2349 1433 1424 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8855
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8855
AvaI C'yCGr_G
Cuts at: 2027 2406 2773 2784 3211 3325 5875 2027
Size: 379 367 11 427 114 2550 5007
Fragments arranged by size:
5007 2550 427 379 367 114 11
BamHI G'GATC_C
Cuts at: 2003 5880 2003
Size: 3877 4978
BanI G'GyrC_C
Cuts at: 1689 2037 3088 3197 3370 3789 4108 4713 4835
Size: 348 1051 109 173 419 319 605 122
Cuts at: 4835 4962 5110 5123 5871 6285 6533 6568 1689
Size: 127 148 13 748 414 248 35 3976
Fragments arranged by size:
3976 1051 748 605 419 414 348 319
248 173 148 127 122 109 35 13
BanII G_rGCy'C
Cuts at: 411 424 1820 2785 3064 3223 4588 5287 5869
Size: 13 1396 965 279 159 1365 699 582
Cuts at: 5869 6899 411
Size: 1030 2367
Fragments arranged by size:
2367 1396 1365 1030 965 699 582 279
159 13
BbeI G_GCGC'C
Cuts at: 2041 3793 4839 6289 6537 2041
Size: 1752 1046 1450 248 4359
Fragments arranged by size:
4359 1752 1450 1046 248
BbsI GAAGACnn'nnnn_
Cuts at: 2124 2849 2124
Size: 725 8130
BbvCI CC'TCA_GC
Cuts at: 239 1154 1636 5020 239
Size: 915 482 3384 4074
Fragments arranged by size:
4074 3384 915 482
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 714 748 778 812 2669 2686 2703 2720 714
Size: 34 30 34 1857 17 17 17 6849
Fragments arranged by size:
6849 1857 34 34 30 17 17 17
BciVI GTATCCnnnnn_n'
Cuts at: 6753 7514 8855 6753
Size: 761 1341 6753
Fragments arranged by size:
6753 1341 761
BclI T'GATC_A
Cuts at: 5087 5420 6375 5087
Size: 333 955 7567
Fragments arranged by size:
7567 955 333
BglI GCCn_nnn'nGGC
Cuts at: 479 1121 3635 479
Size: 642 2514 5699
Fragments arranged by size:
5699 2514 642
BglII A'GATC_T
Cuts at: 2303 5192 5723 6370 8407 2303
Size: 2889 531 647 2037 2751
Fragments arranged by size:
2889 2751 2037 647 531
BlpI GC'TnA_GC
Cuts at: 5286 5669 5967 5286
Size: 383 298 8174
Fragments arranged by size:
8174 383 298
Bme1580I G_kGCm'C
Cuts at: 411 424 727 1131 1414 2221 2590 2785 2958
Size: 13 303 404 283 807 369 195 173
Cuts at: 2958 3091 4588 4793 4967 5227 6480 6573 7629
Size: 133 1497 205 174 260 1253 93 1056
Cuts at: 7629 411
Size: 1637
Fragments arranged by size:
1637 1497 1253 1056 807 404 369 303
283 260 205 195 174 173 133 93
13
BmgBI CAC'GTC
Cuts at: 414 998 1167 1718 2847 5367 414
Size: 584 169 551 1129 2520 3902
Fragments arranged by size:
3902 2520 1129 584 551 169
BmrI ACTGGGnnnn_n'
Cuts at: 315 616 3812 6067 6148 6483 8627 315
Size: 301 3196 2255 81 335 2144 543
Fragments arranged by size:
3196 2255 2144 543 335 301 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5745 7222 5745
Size: 1477 7378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2011 2269 2433 3195 3637 3664 3994 4045 4306
Size: 258 164 762 442 27 330 51 261
Cuts at: 4306 4441 4849 5122 5791 5836 2011
Size: 135 408 273 669 45 5030
Fragments arranged by size:
5030 762 669 442 408 330 273 261
258 164 135 51 45 27
Bpu10I CC'TnA_GC
Cuts at: 239 1111 1154 1246 1636 5020 5689 5842 239
Size: 872 43 92 390 3384 669 153 3252
Fragments arranged by size:
3384 3252 872 669 390 153 92 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 2605 2746 3658 3979 4171 4369 4504 6031
Size: 1773 141 912 321 192 198 135 1527
Cuts at: 6031 6910 7402 7700 832
Size: 879 492 298 1987
Fragments arranged by size:
1987 1773 1527 912 879 492 321 298
198 192 141 135
BsaI GGTCTCn'nnnn_
Cuts at: 1619 2054 3928 5190 5251 8559 1619
Size: 435 1874 1262 61 3308 1915
Fragments arranged by size:
3308 1915 1874 1262 435 61
BsaAI yAC'GTr
Cuts at: 2214 2310 6208 2214
Size: 96 3898 4861
Fragments arranged by size:
4861 3898 96
BsaBI GATnn'nnATC
Cuts at: 6 3100 6369 8406 6
Size: 3094 3269 2037 455
Fragments arranged by size:
3269 3094 2037 455
BsaHI Gr'CG_yC
Cuts at: 1432 1796 1946 2038 2939 2963 3790 4595 4836
Size: 364 150 92 901 24 827 805 241
Cuts at: 4836 4877 4975 5471 6286 6534 7236 8732 1432
Size: 41 98 496 815 248 702 1496 1555
Fragments arranged by size:
1555 1496 901 827 815 805 702 496
364 248 241 150 98 92 41 24
BsaWI w'CCGG_w
Cuts at: 3194 4133 6044 6565 7243 7517 7664 8075 3194
Size: 939 1911 521 678 274 147 411 3974
Fragments arranged by size:
3974 1911 939 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2884 2914 2929 2959 3547 3571 3577 3601 4528
Size: 30 15 30 588 24 6 24 927
Cuts at: 4528 4558 4975 5005 5497 5527 5533 5563 2884
Size: 30 417 30 492 30 6 30 6176
Fragments arranged by size:
6176 927 588 492 417 30 30 30
30 30 30 24 24 15 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 805 4989 805
Size: 4184 4671
BseYI C'CCAG_C
Cuts at: 120 152 457 1143 1338 1820 3260 3516 3537
Size: 32 305 686 195 482 1440 256 21
Cuts at: 3537 3655 4531 4799 5532 5594 6280 7038 7615
Size: 118 876 268 733 62 686 758 577
Cuts at: 7615 8111 120
Size: 496 864
Fragments arranged by size:
1440 876 864 758 733 686 686 577
496 482 305 268 256 195 118 62
32 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 670 1117 1729 1756 1873 1885 2727 2901
Size: 186 447 612 27 117 12 842 174
Cuts at: 2901 3683 4222 5668 484
Size: 782 539 1446 3671
Fragments arranged by size:
3671 1446 842 782 612 539 447 186
174 117 27 12
BsiEI CG_ry'CG
Cuts at: 104 1125 3149 3529 4317 6397 6443 7651 104
Size: 1021 2024 380 788 2080 46 1208 1308
Fragments arranged by size:
2080 2024 1308 1208 1021 788 380 46
BsiHKAI G_wGCw'C
Cuts at: 137 194 635 680 989 2221 3578 3734 4157
Size: 57 441 45 309 1232 1357 156 423
Cuts at: 4157 4187 4385 5528 5869 6647 6837 7629 137
Size: 30 198 1143 341 778 190 792 1363
Fragments arranged by size:
1363 1357 1232 1143 792 778 441 423
341 309 198 190 156 57 45 30
BsiWI C'GTAC_G
Cuts at: 1043 1043
Size: 8855
BsmI GAATG_Cn'
Cuts at: 4051 4707 4051
Size: 656 8199
BsmBI CGTCTCn'nnnn_
Cuts at: 2770 3763 2770
Size: 993 7862
Bsp1286I G_dGCh'C
Cuts at: 137 194 411 424 635 680 727 989 1131
Size: 57 217 13 211 45 47 262 142
Cuts at: 1131 1414 1820 2221 2590 2785 2958 3064 3091
Size: 283 406 401 369 195 173 106 27
Cuts at: 3091 3223 3578 3734 4157 4187 4385 4588 4793
Size: 132 355 156 423 30 198 203 205
Cuts at: 4793 4967 5227 5287 5528 5869 6480 6573 6647
Size: 174 260 60 241 341 611 93 74
Cuts at: 6647 6837 6899 7629 137
Size: 190 62 730 1363
Fragments arranged by size:
1363 730 611 423 406 401 369 355
341 283 262 260 241 217 211 205
203 198 195 190 174 173 156 142
132 106 93 74 62 60 57 47
45 30 27 13
BspEI T'CCGG_A
Cuts at: 8075 8075
Size: 8855
BspHI T'CATG_A
Cuts at: 4340 8031 4340
Size: 3691 5164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 177 1668 1847 2363 2513 2712 3705 3820 3852
Size: 1491 179 516 150 199 993 115 32
Cuts at: 3852 5811 5905 6119 6421 6802 177
Size: 1959 94 214 302 381 2230
Fragments arranged by size:
2230 1959 1491 993 516 381 302 214
199 179 150 115 94 32
BsrBI CCG'CTC
Cuts at: 50 2015 3525 5108 7147 7201 8685 8850 50
Size: 1965 1510 1583 2039 54 1484 165 55
Fragments arranged by size:
2039 1965 1583 1510 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 680 1881 6767 680
Size: 1201 4886 2768
Fragments arranged by size:
4886 2768 1201
BsrFI r'CCGG_y
Cuts at: 979 1115 1510 1568 3155 4111 4716 6853 7034
Size: 136 395 58 1587 956 605 2137 181
Cuts at: 7034 7243 979
Size: 209 2591
Fragments arranged by size:
2591 2137 1587 956 605 395 209 181
136 58
BsrGI T'GTAC_A
Cuts at: 902 3449 902
Size: 2547 6308
BssHII G'CGCG_C
Cuts at: 4852 4852
Size: 8855
BssSI C'ACGA_G
Cuts at: 3974 4084 5719 7126 7484 3974
Size: 110 1635 1407 358 5345
Fragments arranged by size:
5345 1635 1407 358 110
BstAPI GCAn_nnn'nTGC
Cuts at: 1851 2741 4263 5802 6232 1851
Size: 890 1522 1539 430 4474
Fragments arranged by size:
4474 1539 1522 890 430
BstBI TT'CG_AA
Cuts at: 2840 7216 2840
Size: 4376 4479
BstEII G'GTnAC_C
Cuts at: 4743 5164 4743
Size: 421 8434
BstXI CCAn_nnnn'nTGG
Cuts at: 2340 4101 2340
Size: 1761 7094
BstYI r'GATC_y
Cuts at: 1 851 1538 2003 2285 2303 2756 3191 5192
Size: 850 687 465 282 18 453 435 2001
Cuts at: 5192 5351 5723 5756 5880 6347 6370 6705 6951
Size: 159 372 33 124 467 23 335 246
Cuts at: 6951 7963 8049 8061 8407 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2001 1012 850 687 467 465 453 449
435 372 346 335 282 246 159 124
86 33 23 18 12
Bsu36I CC'TnA_GG
Cuts at: 3275 3313 4709 3275
Size: 38 1396 7421
Fragments arranged by size:
7421 1396 38
BtgI C'CryG_G
Cuts at: 175 477 1293 1318 1469 3069 3464 4317 4814
Size: 302 816 25 151 1600 395 853 497
Cuts at: 4814 5169 175
Size: 355 3861
Fragments arranged by size:
3861 1600 853 816 497 395 355 302
151 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5604 6805 6986 7149 5604
Size: 1201 181 163 7310
Fragments arranged by size:
7310 1201 181 163
BtsI GCAGTG_nn'
Cuts at: 368 397 1852 2007 2464 2685 3135 3856 5227
Size: 29 1455 155 457 221 450 721 1371
Cuts at: 5227 6098 368
Size: 871 3125
Fragments arranged by size:
3125 1455 1371 871 721 457 450 221
155 29
ClaI AT'CG_AT
Cuts at: 734 1367 734
Size: 633 8222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3327 3362 4872 4907 3327
Size: 35 1510 35 7275
Fragments arranged by size:
7275 1510 35 35
DraI TTT'AAA
Cuts at: 5854 5854
Size: 8855
DraIII CAC_nnn'GTG
Cuts at: 324 2229 4182 5130 324
Size: 1905 1953 948 4049
Fragments arranged by size:
4049 1953 1905 948
DrdI GACnn_nn'nnGTC
Cuts at: 4876 4944 6561 7419 4876
Size: 68 1617 858 6312
Fragments arranged by size:
6312 1617 858 68
EaeI y'GGCC_r
Cuts at: 296 782 1122 1524 1566 1998 2342 2657 3153
Size: 486 340 402 42 432 344 315 496
Cuts at: 3153 3380 3488 3526 5048 5213 5300 6440 6614
Size: 227 108 38 1522 165 87 1140 174
Cuts at: 6614 7005 7032 8377 296
Size: 391 27 1345 774
Fragments arranged by size:
1522 1345 1140 774 496 486 432 402
391 344 340 315 227 174 165 108
87 42 38 27
EagI C'GGCC_G
Cuts at: 1122 3526 6440 1122
Size: 2404 2914 3537
Fragments arranged by size:
3537 2914 2404
EarI CTCTTCn'nnn_
Cuts at: 604 721 2401 4900 5377 6878 7088 604
Size: 117 1680 2499 477 1501 210 2371
Fragments arranged by size:
2499 2371 1680 1501 477 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2013 3926 4968 7063 7373 7519 8665 2013
Size: 1913 1042 2095 310 146 1146 2203
Fragments arranged by size:
2203 2095 1913 1146 1042 310 146
EcoICRI GAG'CTC
Cuts at: 5867 5867
Size: 8855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2011 2269 2433 3195 3637 3664 3670 3994 4045
Size: 258 164 762 442 27 6 324 51
Cuts at: 4045 4306 4441 4849 5122 5250 5791 5836 6680
Size: 261 135 408 273 128 541 45 844
Cuts at: 6680 7112 7859 8127 2011
Size: 432 747 268 2739
Fragments arranged by size:
2739 844 762 747 541 442 432 408
324 273 268 261 258 164 135 128
51 45 27 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1096 1153 2971 3080 4627 5773 1096
Size: 57 1818 109 1547 1146 4178
Fragments arranged by size:
4178 1818 1547 1146 109 57
EcoO109I rG'GnC_Cy
Cuts at: 149 309 421 1746 1899 3300 3350 4584 4585
Size: 160 112 1325 153 1401 50 1234 1
Cuts at: 4585 4946 5994 149
Size: 361 1048 3010
Fragments arranged by size:
3010 1401 1325 1234 1048 361 160 153
112 50 1
EcoRI G'AATT_C
Cuts at: 2466 5859 2466
Size: 3393 5462
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4074 4275 6874 4074
Size: 201 2599 6055
Fragments arranged by size:
6055 2599 201
FseI GG_CCGG'CC
Cuts at: 1572 3159 1572
Size: 1587 7268
FspI TGC'GCA
Cuts at: 3502 5044 6636 3502
Size: 1542 1592 5721
Fragments arranged by size:
5721 1592 1542
HaeII r_GCGC'y
Cuts at: 560 1827 2041 2667 3027 3233 3793 4839 5714
Size: 1267 214 626 360 206 560 1046 875
Cuts at: 5714 6289 6537 7559 8538 560
Size: 575 248 1022 979 877
Fragments arranged by size:
1267 1046 1022 979 877 875 626 575
560 360 248 214 206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 820 852 853 885 1616 1648 2929
Size: 32 61 32 1 32 731 32 1281
Cuts at: 2929 2961 4933 4965 5497 5529 5533 5565 5646
Size: 32 1972 32 532 32 4 32 81
Cuts at: 5646 5678 6697 6727 6729 6759 727
Size: 32 1019 30 2 30 2823
Fragments arranged by size:
2823 1972 1281 1019 731 532 81 61
32 32 32 32 32 32 32 32
32 30 30 4 2 1
HincII GTy'rAC
Cuts at: 504 1950 2175 2262 5894 504
Size: 1446 225 87 3632 3465
Fragments arranged by size:
3632 3465 1446 225 87
KasI G'GCGC_C
Cuts at: 2037 3789 4835 6285 6533 2037
Size: 1752 1046 1450 248 4359
Fragments arranged by size:
4359 1752 1450 1046 248
KpnI G_GTAC'C
Cuts at: 5875 5875
Size: 8855
MluI A'CGCG_T
Cuts at: 8288 8288
Size: 8855
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3746 4424 5018 5576 7526 7710 3746
Size: 678 594 558 1950 184 4891
Fragments arranged by size:
4891 1950 678 594 558 184
MscI TGG'CCA
Cuts at: 784 3382 3490 5215 5302 6616 784
Size: 2598 108 1725 87 1314 3023
Fragments arranged by size:
3023 2598 1725 1314 108 87
MslI CAynn'nnrTG
Cuts at: 3034 3463 4150 4378 5083 6844 6971 3034
Size: 429 687 228 705 1761 127 4918
Fragments arranged by size:
4918 1761 705 687 429 228 127
MspA1I CmG'CkG
Cuts at: 168 177 960 1143 1857 1942 2067 2669 3344
Size: 9 783 183 714 85 125 602 675
Cuts at: 3344 4242 4319 4392 4542 4803 4816 5171 5298
Size: 898 77 73 150 261 13 355 127
Cuts at: 5298 5799 5966 6280 6640 7653 7898 168
Size: 501 167 314 360 1013 245 1125
Fragments arranged by size:
1125 1013 898 783 714 675 602 501
360 355 314 261 245 183 167 150
127 125 85 77 73 13 9
NaeI GCC'GGC
Cuts at: 981 1117 1512 1570 3157 4113 7036 981
Size: 136 395 58 1587 956 2923 2800
Fragments arranged by size:
2923 2800 1587 956 395 136 58
NarI GG'CG_CC
Cuts at: 2038 3790 4836 6286 6534 2038
Size: 1752 1046 1450 248 4359
Fragments arranged by size:
4359 1752 1450 1046 248
NcoI C'CATG_G
Cuts at: 3464 3464
Size: 8855
NdeI CA'TA_TG
Cuts at: 4140 4140
Size: 8855
NgoMIV G'CCGG_C
Cuts at: 979 1115 1510 1568 3155 4111 7034 979
Size: 136 395 58 1587 956 2923 2800
Fragments arranged by size:
2923 2800 1587 956 395 136 58
NspI r_CATG'y
Cuts at: 234 807 5040 5316 5908 6408 7315 234
Size: 573 4233 276 592 500 907 1774
Fragments arranged by size:
4233 1774 907 592 573 500 276
PasI CC'CwG_GG
Cuts at: 254 564 1188 1404 254
Size: 310 624 216 7705
Fragments arranged by size:
7705 624 310 216
PciI A'CATG_T
Cuts at: 803 5312 7311 803
Size: 4509 1999 2347
Fragments arranged by size:
4509 2347 1999
PflMI CCAn_nnn'nTGG
Cuts at: 305 324 483 1143 1404 2070 5120 305
Size: 19 159 660 261 666 3050 4040
Fragments arranged by size:
4040 3050 666 660 261 159 19
PfoI T'CCnGG_A
Cuts at: 1988 2246 3172 5324 1988
Size: 258 926 2152 5519
Fragments arranged by size:
5519 2152 926 258
PmeI GTTT'AAAC
Cuts at: 5854 5854
Size: 8855
PmlI CAC'GTG
Cuts at: 2214 2214
Size: 8855
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4528 4560 7994 8026 4528
Size: 32 3434 32 5357
Fragments arranged by size:
5357 3434 32 32
PpuMI rG'GwC_Cy
Cuts at: 1746 1899 3300 3350 4946 1746
Size: 153 1401 50 1596 5655
Fragments arranged by size:
5655 1596 1401 153 50
PshAI GACnn'nnGTC
Cuts at: 2849 2849
Size: 8855
PspOMI G'GGCC_C
Cuts at: 407 420 2781 4584 407
Size: 13 2361 1803 4678
Fragments arranged by size:
4678 2361 1803 13
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1036 1068 1036
Size: 32 8823
PstI C_TGCA'G
Cuts at: 188 947 1364 1847 1916 2146 2459 3863 4007
Size: 759 417 483 69 230 313 1404 144
Cuts at: 4007 4073 4355 4868 5234 5902 6587 188
Size: 66 282 513 366 668 685 2456
Fragments arranged by size:
2456 1404 759 685 668 513 483 417
366 313 282 230 144 69 66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8855
PvuII CAG'CTG
Cuts at: 960 1143 1857 1942 4242 4392 6280 6640 960
Size: 183 714 85 2300 150 1888 360 3175
Fragments arranged by size:
3175 2300 1888 714 360 183 150 85
RsrII CG'GwC_CG
Cuts at: 7050 7050
Size: 8855
SacI G_AGCT'C
Cuts at: 5869 5869
Size: 8855
SacII CC_GC'GG
Cuts at: 178 4320 4817 5172 178
Size: 4142 497 355 3861
Fragments arranged by size:
4142 3861 497 355
SalI G'TCGA_C
Cuts at: 5892 5892
Size: 8855
SanDI GG'GwC_CC
Cuts at: 1746 3350 1746
Size: 1604 7251
SapI GCTCTTCn'nnn_
Cuts at: 721 5377 6878 7088 721
Size: 4656 1501 210 2488
Fragments arranged by size:
4656 2488 1501 210
SbfI CC_TGCA'GG
Cuts at: 188 4355 4868 5902 188
Size: 4167 513 1034 3141
Fragments arranged by size:
4167 3141 1034 513
ScaI AGT'ACT
Cuts at: 492 751 8372 492
Size: 259 7621 975
Fragments arranged by size:
7621 975 259
SexAI A'CCwGG_T
Cuts at: 4931 5126 4931
Size: 195 8660
SfcI C'TryA_G
Cuts at: 33 184 943 1360 1843 1912 2142 2455 3859
Size: 151 759 417 483 69 230 313 1404
Cuts at: 3859 4003 4069 4351 4864 5230 5898 6583 7576
Size: 144 66 282 513 366 668 685 993
Cuts at: 7576 7767 33
Size: 191 1121
Fragments arranged by size:
1404 1121 993 759 685 668 513 483
417 366 313 282 230 191 151 144
69 66
SfoI GGC'GCC
Cuts at: 2039 3791 4837 6287 6535 2039
Size: 1752 1046 1450 248 4359
Fragments arranged by size:
4359 1752 1450 1046 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8855
SgrAI Cr'CCGG_yG
Cuts at: 979 979
Size: 8855
SmaI CCC'GGG
Cuts at: 2029 2408 3213 5877 2029
Size: 379 805 2664 5007
Fragments arranged by size:
5007 2664 805 379
SmlI C'TyrA_G
Cuts at: 847 2620 2761 3673 3994 4186 4384 4519 6010
Size: 1773 141 912 321 192 198 135 1491
Cuts at: 6010 6925 7417 7679 847
Size: 915 492 262 2023
Fragments arranged by size:
2023 1773 1491 915 912 492 321 262
198 192 141 135
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8855
SphI G_CATG'C
Cuts at: 5040 5908 6408 5040
Size: 868 500 7487
Fragments arranged by size:
7487 868 500
StuI AGG'CCT
Cuts at: 1918 1918
Size: 8855
StyI C'CwwG_G
Cuts at: 145 2093 3464 4754 4808 5219 5570 5989 6343
Size: 1948 1371 1290 54 411 351 419 354
Cuts at: 6343 145
Size: 2657
Fragments arranged by size:
2657 1948 1371 1290 419 411 354 351
54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 331 598 744 1684 3033 3135 4762 5400 5586
Size: 267 146 940 1349 102 1627 638 186
Cuts at: 5586 6438 6571 7030 7239 331
Size: 852 133 459 209 1947
Fragments arranged by size:
1947 1627 1349 940 852 638 459 267
209 186 146 133 102
TatI w'GTAC_w
Cuts at: 490 749 902 1712 2900 3284 3449 3866 4553
Size: 259 153 810 1188 384 165 417 687
Cuts at: 4553 5309 5744 8370 490
Size: 756 435 2626 975
Fragments arranged by size:
2626 1188 975 810 756 687 435 417
384 259 165 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 625 657 2549 2581 2639 2671 3171 3203 4816
Size: 32 1892 32 58 32 500 32 1613
Cuts at: 4816 4848 8372 8404 625
Size: 32 3524 32 1076
Fragments arranged by size:
3524 1892 1613 1076 500 58 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 499 1630 2128 4461 4741 6652 499
Size: 1131 498 2333 280 1911 2702
Fragments arranged by size:
2702 2333 1911 1131 498 280
XbaI T'CTAG_A
Cuts at: 5886 5886
Size: 8855
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 717 3639 6350 717
Size: 2922 2711 3222
Fragments arranged by size:
3222 2922 2711
XmaI C'CCGG_G
Cuts at: 2027 2406 3211 5875 2027
Size: 379 805 2664 5007
Fragments arranged by size:
5007 2664 805 379
XmnI GAAnn'nnTTC
Cuts at: 4258 4258
Size: 8855
ZraI GAC'GTC
Cuts at: 4878 4976 4878
Size: 98 8757
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BfrBI BmtI BstZ17I EcoRV
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PsiI PspXI SfiI SnaBI SrfI SspI SwaI
XhoI