Restriction map for
pF1KB7015
(Circular) (Six-base) MAPSORT of: pF1KB7015.seq Check: 3238 from: 1 to: 5531
pF1KB7015 5531 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 381 796 1129 1858 381
Size: 415 333 729 4054
Fragments arranged by size:
4054 729 415 333
AatII G_ACGT'C
Cuts at: 2138 2374 2138
Size: 236 5295
AccI GT'mk_AC
Cuts at: 2117 2569 2117
Size: 452 5079
Acc65I G'GTAC_C
Cuts at: 2547 2547
Size: 5531
AclI AA'CG_TT
Cuts at: 5371 5371
Size: 5531
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 694 2248 3356 3788 4141 4803 453
Size: 241 1554 1108 432 353 662 1181
Fragments arranged by size:
1554 1181 1108 662 432 353 241
AfeI AGC'GCT
Cuts at: 850 850
Size: 5531
AflIII A'CryG_T
Cuts at: 1391 2883 4683 4964 1391
Size: 1492 1800 281 1958
Fragments arranged by size:
1958 1800 1492 281
AhdI GACnn_n'nnGTC
Cuts at: 1674 1890 2139 1674
Size: 216 249 5066
Fragments arranged by size:
5066 249 216
AleI CACnn'nnGTG
Cuts at: 470 541 2129 470
Size: 71 1588 3872
Fragments arranged by size:
3872 1588 71
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 739 739
Size: 5531
AlwNI CAG_nnn'CTG
Cuts at: 387 396 643 2836 4274 387
Size: 9 247 2193 1438 1644
Fragments arranged by size:
2193 1644 1438 247 9
ApaI G_GGCC'C
Cuts at: 229 320 380 558 1006 1453 1518 1757 229
Size: 91 60 178 448 447 65 239 4003
Fragments arranged by size:
4003 448 447 239 178 91 65 60
ApaLI G'TGCA_C
Cuts at: 1339 2032 4369 1339
Size: 693 2337 2501
Fragments arranged by size:
2501 2337 693
ApoI r'AATT_y
Cuts at: 2535 4884 5140 2535
Size: 2349 256 2926
Fragments arranged by size:
2926 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5531
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5531
AvaI C'yCGr_G
Cuts at: 222 298 498 574 1477 1511 1824 1861 1913
Size: 76 200 76 903 34 313 37 52
Cuts at: 1913 2024 2421 2551 222
Size: 111 397 130 3202
Fragments arranged by size:
3202 903 397 313 200 130 111 76
76 52 37 34
BamHI G'GATC_C
Cuts at: 2556 2556
Size: 5531
BanI G'GyrC_C
Cuts at: 112 163 351 762 1735 2091 2400 2547 2961
Size: 51 188 411 973 356 309 147 414
Cuts at: 2961 3209 3244 112
Size: 248 35 2399
Fragments arranged by size:
2399 973 414 411 356 309 248 188
147 51 35
BanII G_rGCy'C
Cuts at: 229 236 320 380 558 1006 1330 1405 1453
Size: 7 84 60 178 448 324 75 48
Cuts at: 1453 1478 1518 1757 2185 2216 2518 2545 3575
Size: 25 40 239 428 31 302 27 1030
Cuts at: 3575 229
Size: 2185
Fragments arranged by size:
2185 1030 448 428 324 302 239 178
84 75 60 48 40 31 27 25
7
BbeI G_GCGC'C
Cuts at: 116 167 1739 2095 2404 2965 3213 116
Size: 51 1572 356 309 561 248 2434
Fragments arranged by size:
2434 1572 561 356 309 248 51
BbsI GAAGACnn'nnnn_
Cuts at: 249 518 2062 249
Size: 269 1544 3718
Fragments arranged by size:
3718 1544 269
BbvCI CC'TCA_GC
Cuts at: 530 1237 530
Size: 707 4824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1468 1502 1468
Size: 34 5497
BciVI GTATCCnnnnn_n'
Cuts at: 3429 4485 5531 3429
Size: 1056 1046 3429
Fragments arranged by size:
3429 1056 1046
BclI T'GATC_A
Cuts at: 2282 3051 2282
Size: 769 4762
BglI GCCn_nnn'nGGC
Cuts at: 157 384 607 1010 1130 157
Size: 227 223 403 120 4558
Fragments arranged by size:
4558 403 227 223 120
BglII A'GATC_T
Cuts at: 1346 2165 3046 5083 1346
Size: 819 881 2037 1794
Fragments arranged by size:
2037 1794 881 819
BlpI GC'TnA_GC
Cuts at: 283 2643 283
Size: 2360 3171
Bme1580I G_kGCm'C
Cuts at: 229 320 356 380 558 767 1006 1343 1453
Size: 91 36 24 178 209 239 337 110
Cuts at: 1453 1518 1757 2036 3156 3249 4373 229
Size: 65 239 279 1120 93 1124 1387
Fragments arranged by size:
1387 1124 1120 337 279 239 239 209
178 110 93 91 65 36 24
BmgBI CAC'GTC
Cuts at: 840 918 2031 840
Size: 78 1113 4340
Fragments arranged by size:
4340 1113 78
BmrI ACTGGGnnnn_n'
Cuts at: 2743 2824 3159 5303 2743
Size: 81 335 2144 2971
Fragments arranged by size:
2971 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1095 2433 3898 1095
Size: 1338 1465 2728
Fragments arranged by size:
2728 1465 1338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1402 2430 865
Size: 537 1028 3966
Fragments arranged by size:
3966 1028 537
Bpu10I CC'TnA_GC
Cuts at: 530 1237 530
Size: 707 4824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 1018 1348 2707 3586 4300 4598 955
Size: 63 330 1359 879 714 298 1888
Fragments arranged by size:
1888 1359 879 714 330 298 63
BsaI GGTCTCn'nnnn_
Cuts at: 5235 5235
Size: 5531
BsaAI yAC'GTr
Cuts at: 978 1764 2884 978
Size: 786 1120 3625
Fragments arranged by size:
3625 1120 786
BsaBI GATnn'nnATC
Cuts at: 6 2002 3045 5082 6
Size: 1996 1043 2037 455
Fragments arranged by size:
2037 1996 1043 455
BsaHI Gr'CG_yC
Cuts at: 113 164 1106 1736 2092 2135 2371 2401 2962
Size: 51 942 630 356 43 236 30 561
Cuts at: 2962 3210 3912 5408 113
Size: 248 702 1496 236
Fragments arranged by size:
1496 942 702 630 561 356 248 236
236 51 43 30
BsaWI w'CCGG_w
Cuts at: 2720 3241 3919 4330 4477 4751 2720
Size: 521 678 411 147 274 3500
Fragments arranged by size:
3500 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 400 430 474 504 790 820 1333 1363 1918
Size: 30 44 30 286 30 513 30 555
Cuts at: 1918 1948 400
Size: 30 3983
Fragments arranged by size:
3983 555 513 286 44 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 159 1201 1204 1879 2224 2442 159
Size: 1042 3 675 345 218 3248
Fragments arranged by size:
3248 1042 675 345 218 3
BseYI C'CCAG_C
Cuts at: 380 636 906 1731 2175 2440 2503 2956 3714
Size: 256 270 825 444 265 63 453 758
Cuts at: 3714 4379 4787 380
Size: 665 408 1124
Fragments arranged by size:
1124 825 758 665 453 444 408 270
265 256 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1725 2247 1725
Size: 522 5009
BsiEI CG_ry'CG
Cuts at: 104 611 2070 3073 3119 4349 104
Size: 507 1459 1003 46 1230 1286
Fragments arranged by size:
1459 1286 1230 1003 507 46
BsiHKAI G_wGCw'C
Cuts at: 704 1034 1343 1405 1478 2036 2216 2545 3323
Size: 330 309 62 73 558 180 329 778
Cuts at: 3323 3513 4373 704
Size: 190 860 1862
Fragments arranged by size:
1862 860 778 558 330 329 309 190
180 73 62
BsmI GAATG_Cn'
Cuts at: 405 405
Size: 5531
Bsp1286I G_dGCh'C
Cuts at: 229 236 320 356 380 558 704 767 1006
Size: 7 84 36 24 178 146 63 239
Cuts at: 1006 1034 1330 1343 1405 1453 1478 1518 1757
Size: 28 296 13 62 48 25 40 239
Cuts at: 1757 2036 2185 2216 2518 2545 3156 3249 3323
Size: 279 149 31 302 27 611 93 74
Cuts at: 3323 3513 3575 4373 229
Size: 190 62 798 1387
Fragments arranged by size:
1387 798 611 302 296 279 239 239
190 178 149 146 93 84 74 63
62 62 48 40 36 31 28 27
25 24 13 7
BspHI T'CATG_A
Cuts at: 3963 3963
Size: 5531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 381 796 1129 1736 1802 1858 2492 2581 2795
Size: 415 333 607 66 56 634 89 214
Cuts at: 2795 3097 3478 381
Size: 302 381 2434
Fragments arranged by size:
2434 634 607 415 381 333 302 214
89 66 56
BsrBI CCG'CTC
Cuts at: 50 211 579 1362 3823 3877 5361 5526 50
Size: 161 368 783 2461 54 1484 165 55
Fragments arranged by size:
2461 1484 783 368 165 161 55 54
BsrDI GCAATG_nn'
Cuts at: 1977 3443 1977
Size: 1466 4065
BsrFI r'CCGG_y
Cuts at: 160 434 606 860 2069 3529 3710 160
Size: 274 172 254 1209 1460 181 1981
Fragments arranged by size:
1981 1460 1209 274 254 181 172
BsrGI T'GTAC_A
Cuts at: 938 2276 938
Size: 1338 4193
BssSI C'ACGA_G
Cuts at: 409 3802 4510 409
Size: 3393 708 1430
Fragments arranged by size:
3393 1430 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2908 2908
Size: 5531
BstBI TT'CG_AA
Cuts at: 3892 3892
Size: 5531
BstEII G'GTnAC_C
Cuts at: 1789 1980 1789
Size: 191 5340
BstYI r'GATC_y
Cuts at: 1 1346 2165 2556 3023 3046 3381 3627 3933
Size: 1345 819 391 467 23 335 246 306
Cuts at: 3933 3945 4031 5083 1
Size: 12 86 1052 449
Fragments arranged by size:
1345 1052 819 467 449 391 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1440 2096 2454 1440
Size: 656 358 4517
Fragments arranged by size:
4517 656 358
BtgI C'CryG_G
Cuts at: 107 146 311 693 774 107
Size: 39 165 382 81 4864
Fragments arranged by size:
4864 382 165 81 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1410 3481 3662 3825 1410
Size: 2071 181 163 3116
Fragments arranged by size:
3116 2071 181 163
BtsI GCAGTG_nn'
Cuts at: 742 927 1224 2514 2774 742
Size: 185 297 1290 260 3499
Fragments arranged by size:
3499 1290 297 260 185
DraI TTT'AAA
Cuts at: 2530 2530
Size: 5531
DrdI GACnn_nn'nnGTC
Cuts at: 3237 4581 3237
Size: 1344 4187
EaeI y'GGCC_r
Cuts at: 143 149 158 237 599 608 887 1460 1557
Size: 6 9 79 362 9 279 573 97
Cuts at: 1557 1781 2067 2427 3116 3290 3681 3708 5053
Size: 224 286 360 689 174 391 27 1345
Cuts at: 5053 143
Size: 621
Fragments arranged by size:
1345 689 621 573 391 362 360 286
279 224 174 97 79 27 9 9
6
EagI C'GGCC_G
Cuts at: 608 2067 3116 608
Size: 1459 1049 3023
Fragments arranged by size:
3023 1459 1049
EarI CTCTTCn'nnn_
Cuts at: 2221 3554 3764 2221
Size: 1333 210 3988
Fragments arranged by size:
3988 1333 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1588 3739 4481 4627 5341 1588
Size: 2151 742 146 714 1778
Fragments arranged by size:
2151 1778 742 714 146
EcoICRI GAG'CTC
Cuts at: 1403 1476 2214 2543 1403
Size: 73 738 329 4391
Fragments arranged by size:
4391 738 329 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 694 865 1402 2248 2430 3356 3788 4141
Size: 241 171 537 846 182 926 432 353
Cuts at: 4141 4803 453
Size: 662 1181
Fragments arranged by size:
1181 926 846 662 537 432 353 241
182 171
EcoNI CCTnn'n_nnAGG
Cuts at: 1543 2316 1543
Size: 773 4758
EcoO109I rG'GnC_Cy
Cuts at: 316 376 377 422 554 555 1449 1450 1659
Size: 60 1 45 132 1 894 1 209
Cuts at: 1659 1754 1884 1956 2151 2346 2670 316
Size: 95 130 72 195 195 324 3177
Fragments arranged by size:
3177 894 324 209 195 195 132 130
95 72 60 45 1 1 1
EcoRI G'AATT_C
Cuts at: 2535 2535
Size: 5531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3550 3550
Size: 5531
FspI TGC'GCA
Cuts at: 1303 1423 3312 1303
Size: 120 1889 3522
Fragments arranged by size:
3522 1889 120
FspAI rTGC'GCAy
Cuts at: 1423 1423
Size: 5531
HaeII r_GCGC'y
Cuts at: 116 167 852 1049 1739 2095 2404 2965 3213
Size: 51 685 197 690 356 309 561 248
Cuts at: 3213 4443 5214 116
Size: 1230 771 433
Fragments arranged by size:
1230 771 690 685 561 433 356 309
248 197 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 474 478 506 510 1660 1692 1876 1908 2125
Size: 4 28 4 1150 32 184 32 217
Cuts at: 2125 2157 3373 3403 3405 3435 474
Size: 32 1216 30 2 30 2570
Fragments arranged by size:
2570 1216 1150 217 184 32 32 32
30 30 28 4 4 2
HincII GTy'rAC
Cuts at: 2570 2570
Size: 5531
KasI G'GCGC_C
Cuts at: 112 163 1735 2091 2400 2961 3209 112
Size: 51 1572 356 309 561 248 2434
Fragments arranged by size:
2434 1572 561 356 309 248 51
KpnI G_GTAC'C
Cuts at: 2551 2551
Size: 5531
MluI A'CGCG_T
Cuts at: 1391 4964 1391
Size: 3573 1958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1412 1430 1646 4290 4474 1412
Size: 18 216 2644 184 2469
Fragments arranged by size:
2644 2469 216 184 18
MscI TGG'CCA
Cuts at: 151 1462 2429 3292 151
Size: 1311 967 863 2390
Fragments arranged by size:
2390 1311 967 863
MslI CAynn'nnrTG
Cuts at: 470 541 1270 2129 2203 2284 3520 3647 470
Size: 71 729 859 74 81 1236 127 2354
Fragments arranged by size:
2354 1236 859 729 127 81 74 71
MspA1I CmG'CkG
Cuts at: 266 384 431 441 632 643 695 776 2337
Size: 118 47 10 191 11 52 81 1561
Cuts at: 2337 2642 2956 3316 4100 4345 266
Size: 305 314 360 784 245 1452
Fragments arranged by size:
1561 1452 784 360 314 305 245 191
118 81 52 47 11 10
NaeI GCC'GGC
Cuts at: 162 436 608 2071 3712 162
Size: 274 172 1463 1641 1981
Fragments arranged by size:
1981 1641 1463 274 172
NarI GG'CG_CC
Cuts at: 113 164 1736 2092 2401 2962 3210 113
Size: 51 1572 356 309 561 248 2434
Fragments arranged by size:
2434 1572 561 356 309 248 51
NcoI C'CATG_G
Cuts at: 107 311 107
Size: 204 5327
NgoMIV G'CCGG_C
Cuts at: 160 434 606 2069 3710 160
Size: 274 172 1463 1641 1981
Fragments arranged by size:
1981 1641 1463 274 172
NspI r_CATG'y
Cuts at: 2305 2584 3084 4687 2305
Size: 279 500 1603 3149
Fragments arranged by size:
3149 1603 500 279
PasI CC'CwG_GG
Cuts at: 1527 1527
Size: 5531
PciI A'CATG_T
Cuts at: 4683 4683
Size: 5531
PflMI CCAn_nnn'nTGG
Cuts at: 326 643 668 2244 326
Size: 317 25 1576 3613
Fragments arranged by size:
3613 1576 317 25
PmeI GTTT'AAAC
Cuts at: 2530 2530
Size: 5531
PmlI CAC'GTG
Cuts at: 978 978
Size: 5531
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 3977
Size: 32 5499
PpuMI rG'GwC_Cy
Cuts at: 1659 1884 1956 2151 2346 1659
Size: 225 72 195 195 4844
Fragments arranged by size:
4844 225 195 195 72
PshAI GACnn'nnGTC
Cuts at: 1374 1374
Size: 5531
PspOMI G'GGCC_C
Cuts at: 225 316 376 554 1002 1449 1514 1753 225
Size: 91 60 178 448 447 65 239 4003
Fragments arranged by size:
4003 448 447 239 178 91 65 60
PspXI vC'TCGA_Gb
Cuts at: 1477 1477
Size: 5531
PstI C_TGCA'G
Cuts at: 392 875 2578 3263 392
Size: 483 1703 685 2660
Fragments arranged by size:
2660 1703 685 483
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5531
PvuII CAG'CTG
Cuts at: 431 2337 2956 3316 431
Size: 1906 619 360 2646
Fragments arranged by size:
2646 1906 619 360
RsrII CG'GwC_CG
Cuts at: 771 3726 771
Size: 2955 2576
SacI G_AGCT'C
Cuts at: 1405 1478 2216 2545 1405
Size: 73 738 329 4391
Fragments arranged by size:
4391 738 329 73
SacII CC_GC'GG
Cuts at: 696 777 696
Size: 81 5450
SalI G'TCGA_C
Cuts at: 2568 2568
Size: 5531
SapI GCTCTTCn'nnn_
Cuts at: 2221 3554 3764 2221
Size: 1333 210 3988
Fragments arranged by size:
3988 1333 210
SbfI CC_TGCA'GG
Cuts at: 875 2578 875
Size: 1703 3828
ScaI AGT'ACT
Cuts at: 1927 2416 5048 1927
Size: 489 2632 2410
Fragments arranged by size:
2632 2410 489
SexAI A'CCwGG_T
Cuts at: 1670 1886 1904 1670
Size: 216 18 5297
Fragments arranged by size:
5297 216 18
SfcI C'TryA_G
Cuts at: 33 388 426 871 2574 3259 4227 4418 33
Size: 355 38 445 1703 685 968 191 1146
Fragments arranged by size:
1703 1146 968 685 445 355 191 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 157 607 157
Size: 450 5081
SfoI GGC'GCC
Cuts at: 114 165 1737 2093 2402 2963 3211 114
Size: 51 1572 356 309 561 248 2434
Fragments arranged by size:
2434 1572 561 356 309 248 51
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5531
SmaI CCC'GGG
Cuts at: 224 300 1826 1915 2026 2423 2553 224
Size: 76 1526 89 111 397 130 3202
Fragments arranged by size:
3202 1526 397 130 111 89 76
SmlI C'TyrA_G
Cuts at: 970 1033 1363 1477 2686 3601 4315 4577 970
Size: 63 330 114 1209 915 714 262 1924
Fragments arranged by size:
1924 1209 915 714 330 262 114 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5531
SphI G_CATG'C
Cuts at: 2305 2584 3084 2305
Size: 279 500 4752
Fragments arranged by size:
4752 500 279
StuI AGG'CCT
Cuts at: 1322 2101 1322
Size: 779 4752
StyI C'CwwG_G
Cuts at: 107 179 311 1539 1784 2665 3019 107
Size: 72 132 1228 245 881 354 2619
Fragments arranged by size:
2619 1228 881 354 245 132 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1999 2126 2517 3114 3247 3706 3915 1999
Size: 127 391 597 133 459 209 3615
Fragments arranged by size:
3615 597 459 391 209 133 127
TatI w'GTAC_w
Cuts at: 938 1115 1925 2276 2414 5046 938
Size: 177 810 351 138 2632 1423
Fragments arranged by size:
2632 1423 810 351 177 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 400 432 1333 1365 5048 5080 400
Size: 32 901 32 3683 32 851
Fragments arranged by size:
3683 901 851 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2113 2356 3328 2113
Size: 243 972 4316
Fragments arranged by size:
4316 972 243
XbaI T'CTAG_A
Cuts at: 2562 2562
Size: 5531
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1320 1413 3026 1320
Size: 93 1613 3825
Fragments arranged by size:
3825 1613 93
XhoI C'TCGA_G
Cuts at: 1477 1477
Size: 5531
XmaI C'CCGG_G
Cuts at: 222 298 1824 1913 2024 2421 2551 222
Size: 76 1526 89 111 397 130 3202
Fragments arranged by size:
3202 1526 397 130 111 89 76
ZraI GAC'GTC
Cuts at: 2136 2372 2136
Size: 236 5295
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmBI BspEI BssHII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PsiI PsrI SanDI
SgrAI SnaBI SrfI SspI SwaI XmnI