Restriction map for
pF1KB7016
(Circular) (Six-base) MAPSORT of: pF1KB7016.seq Check: 2697 from: 1 to: 5618
pF1KB7016 5618 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 799 2072 799
Size: 1273 4345
AccI GT'mk_AC
Cuts at: 2656 2656
Size: 5618
Acc65I G'GTAC_C
Cuts at: 1936 2634 1936
Size: 698 4920
AclI AA'CG_TT
Cuts at: 5458 5458
Size: 5618
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2152 3443 3875 4228 4890 2152
Size: 1291 432 353 662 2880
Fragments arranged by size:
2880 1291 662 432 353
AfeI AGC'GCT
Cuts at: 202 2476 202
Size: 2274 3344
AflIII A'CryG_T
Cuts at: 2970 4770 5051 2970
Size: 1800 281 3537
Fragments arranged by size:
3537 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 2454 2454
Size: 5618
AleI CACnn'nnGTG
Cuts at: 448 2179 448
Size: 1731 3887
AlwNI CAG_nnn'CTG
Cuts at: 602 1366 1714 2586 2923 4361 602
Size: 764 348 872 337 1438 1859
Fragments arranged by size:
1859 1438 872 764 348 337
ApaI G_GGCC'C
Cuts at: 172 389 1233 1400 1822 172
Size: 217 844 167 422 3968
Fragments arranged by size:
3968 844 422 217 167
ApaLI G'TGCA_C
Cuts at: 4456 4456
Size: 5618
ApoI r'AATT_y
Cuts at: 2622 4971 5227 2622
Size: 2349 256 3013
Fragments arranged by size:
3013 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5618
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5618
AvaI C'yCGr_G
Cuts at: 165 171 328 414 707 1959 2638 165
Size: 6 157 86 293 1252 679 3145
Fragments arranged by size:
3145 1252 679 293 157 86 6
BamHI G'GATC_C
Cuts at: 1248 2643 1248
Size: 1395 4223
BanI G'GyrC_C
Cuts at: 271 442 1560 1735 1936 2314 2634 3048 3296
Size: 171 1118 175 201 378 320 414 248
Cuts at: 3296 3331 271
Size: 35 2558
Fragments arranged by size:
2558 1118 414 378 320 248 201 175
171 35
BanII G_rGCy'C
Cuts at: 172 184 341 389 1164 1233 1392 1400 1417
Size: 12 157 48 775 69 159 8 17
Cuts at: 1417 1496 1800 1822 2543 2632 3662 172
Size: 79 304 22 721 89 1030 2128
Fragments arranged by size:
2128 1030 775 721 304 159 157 89
79 69 48 22 17 12 8
BbeI G_GCGC'C
Cuts at: 275 3052 3300 275
Size: 2777 248 2593
Fragments arranged by size:
2777 2593 248
BbsI GAAGACnn'nnnn_
Cuts at: 1614 1623 1614
Size: 9 5609
BbvCI CC'TCA_GC
Cuts at: 240 1868 1941 2152 240
Size: 1628 73 211 3706
Fragments arranged by size:
3706 1628 211 73
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 128 162 2538 2572 128
Size: 34 2376 34 3174
Fragments arranged by size:
3174 2376 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3516 4572 5618 3516
Size: 1056 1046 3516
Fragments arranged by size:
3516 1056 1046
BclI T'GATC_A
Cuts at: 3138 3138
Size: 5618
BglI GCCn_nnn'nGGC
Cuts at: 112 121 217 1159 112
Size: 9 96 942 4571
Fragments arranged by size:
4571 942 96 9
BglII A'GATC_T
Cuts at: 3133 5170 3133
Size: 2037 3581
BlpI GC'TnA_GC
Cuts at: 2730 2730
Size: 5618
Bme1580I G_kGCm'C
Cuts at: 172 389 1233 1400 1740 1822 2343 3243 3336
Size: 217 844 167 340 82 521 900 93
Cuts at: 3336 4460 172
Size: 1124 1330
Fragments arranged by size:
1330 1124 900 844 521 340 217 167
93 82
BmgBI CAC'GTC
Cuts at: 2451 2451
Size: 5618
BmrI ACTGGGnnnn_n'
Cuts at: 701 2830 2911 3246 5390 701
Size: 2129 81 335 2144 929
Fragments arranged by size:
2144 2129 929 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1956 3985 1956
Size: 2029 3589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 313 1111 1186 1936 2332 304
Size: 9 798 75 750 396 3590
Fragments arranged by size:
3590 798 750 396 75 9
Bpu10I CC'TnA_GC
Cuts at: 240 381 1402 1524 1868 1908 1941 2152 240
Size: 141 1021 122 344 40 33 211 3706
Fragments arranged by size:
3706 1021 344 211 141 122 40 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1219 1467 2794 3673 4387 4685 1219
Size: 248 1327 879 714 298 2152
Fragments arranged by size:
2152 1327 879 714 298 248
BsaI GGTCTCn'nnnn_
Cuts at: 625 1819 5322 625
Size: 1194 3503 921
Fragments arranged by size:
3503 1194 921
BsaAI yAC'GTr
Cuts at: 2971 2971
Size: 5618
BsaBI GATnn'nnATC
Cuts at: 6 1000 3132 5169 6
Size: 994 2132 2037 455
Fragments arranged by size:
2132 2037 994 455
BsaHI Gr'CG_yC
Cuts at: 250 272 542 3049 3297 3999 5495 250
Size: 22 270 2507 248 702 1496 373
Fragments arranged by size:
2507 1496 702 373 270 248 22
BsaWI w'CCGG_w
Cuts at: 2807 3328 4006 4417 4564 4838 2807
Size: 521 678 411 147 274 3587
Fragments arranged by size:
3587 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 610 640 2344 2374 610
Size: 30 1704 30 3854
Fragments arranged by size:
3854 1704 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1786 1789 2107 2152 1786
Size: 3 318 45 5252
Fragments arranged by size:
5252 318 45 3
BseYI C'CCAG_C
Cuts at: 588 3043 3801 4466 4874 588
Size: 2455 758 665 408 1332
Fragments arranged by size:
2455 1332 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 549 1207 1352 1462 1508 2186 2473 330
Size: 219 658 145 110 46 678 287 3475
Fragments arranged by size:
3475 678 658 287 219 145 110 46
BsiEI CG_ry'CG
Cuts at: 104 152 221 3160 3206 4436 104
Size: 48 69 2939 46 1230 1286
Fragments arranged by size:
2939 1286 1230 69 48 46
BsiHKAI G_wGCw'C
Cuts at: 299 616 928 1139 2123 2632 3410 3600 4460
Size: 317 312 211 984 509 778 190 860
Cuts at: 4460 299
Size: 1457
Fragments arranged by size:
1457 984 860 778 509 317 312 211
190
BsmBI CGTCTCn'nnnn_
Cuts at: 1771 2479 1771
Size: 708 4910
Bsp1286I G_dGCh'C
Cuts at: 172 184 299 341 389 616 928 1139 1164
Size: 12 115 42 48 227 312 211 25
Cuts at: 1164 1233 1392 1400 1417 1496 1740 1800 1822
Size: 69 159 8 17 79 244 60 22
Cuts at: 1822 2123 2343 2543 2632 3243 3336 3410 3600
Size: 301 220 200 89 611 93 74 190
Cuts at: 3600 3662 4460 172
Size: 62 798 1330
Fragments arranged by size:
1330 798 611 312 301 244 227 220
211 200 190 159 115 93 89 79
74 69 62 60 48 42 25 22
17 12 8
BspHI T'CATG_A
Cuts at: 1013 4050 1013
Size: 3037 2581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 799 2072 2668 2882 3184 3565 799
Size: 1273 596 214 302 381 2852
Fragments arranged by size:
2852 1273 596 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1095 1523 1544 3910 3964 5448 5613 50
Size: 1045 428 21 2366 54 1484 165 55
Fragments arranged by size:
2366 1484 1045 428 165 55 54 21
BsrDI GCAATG_nn'
Cuts at: 913 3530 913
Size: 2617 3001
BsrFI r'CCGG_y
Cuts at: 1699 2067 3616 3797 1699
Size: 368 1549 181 3520
Fragments arranged by size:
3520 1549 368 181
BssSI C'ACGA_G
Cuts at: 1565 3889 4597 1565
Size: 2324 708 2586
Fragments arranged by size:
2586 2324 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1143 2995 1143
Size: 1852 3766
BstBI TT'CG_AA
Cuts at: 3979 3979
Size: 5618
BstXI CCAn_nnnn'nTGG
Cuts at: 2311 2311
Size: 5618
BstYI r'GATC_y
Cuts at: 1 1118 1248 2322 2643 3110 3133 3468 3714
Size: 1117 130 1074 321 467 23 335 246
Cuts at: 3714 4020 4032 4118 5170 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1117 1074 1052 467 449 335 321 306
246 130 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1931 1931
Size: 5618
BtgI C'CryG_G
Cuts at: 107 119 245 260 389 446 1253 1373 1433
Size: 12 126 15 129 57 807 120 60
Cuts at: 1433 107
Size: 4292
Fragments arranged by size:
4292 807 129 126 120 60 57 15
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3568 3749 3912 3568
Size: 181 163 5274
Fragments arranged by size:
5274 181 163
BtsI GCAGTG_nn'
Cuts at: 297 857 2861 297
Size: 560 2004 3054
Fragments arranged by size:
3054 2004 560
DraI TTT'AAA
Cuts at: 2617 2617
Size: 5618
DraIII CAC_nnn'GTG
Cuts at: 1894 1894
Size: 5618
DrdI GACnn_nn'nnGTC
Cuts at: 3324 4668 3324
Size: 1344 4274
EaeI y'GGCC_r
Cuts at: 149 218 998 1701 1757 2186 3203 3377 3768
Size: 69 780 703 56 429 1017 174 391
Cuts at: 3768 3795 5140 149
Size: 27 1345 627
Fragments arranged by size:
1345 1017 780 703 627 429 391 174
69 56 27
EagI C'GGCC_G
Cuts at: 149 218 3203 149
Size: 69 2985 2564
Fragments arranged by size:
2985 2564 69
EarI CTCTTCn'nnn_
Cuts at: 916 3641 3851 916
Size: 2725 210 2683
Fragments arranged by size:
2725 2683 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 940 3826 4568 4714 5428 940
Size: 2886 742 146 714 1130
Fragments arranged by size:
2886 1130 742 714 146
EcoICRI GAG'CTC
Cuts at: 2630 2630
Size: 5618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 313 1111 1186 1936 2152 2332 3443 3875
Size: 9 798 75 750 216 180 1111 432
Cuts at: 3875 4228 4890 304
Size: 353 662 1032
Fragments arranged by size:
1111 1032 798 750 662 432 353 216
180 75 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2134 2134
Size: 5618
EcoO109I rG'GnC_Cy
Cuts at: 385 1230 1287 1363 1396 1397 1818 1819 1858
Size: 845 57 76 33 1 421 1 39
Cuts at: 1858 1873 2757 385
Size: 15 884 3246
Fragments arranged by size:
3246 884 845 421 76 57 39 33
15 1 1
EcoRI G'AATT_C
Cuts at: 2622 2622
Size: 5618
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3637 3637
Size: 5618
FspI TGC'GCA
Cuts at: 1555 3399 1555
Size: 1844 3774
HaeII r_GCGC'y
Cuts at: 204 275 283 321 894 1089 1685 2478 3052
Size: 71 8 38 573 195 596 793 574
Cuts at: 3052 3300 4530 5301 204
Size: 248 1230 771 521
Fragments arranged by size:
1230 793 771 596 574 573 521 248
195 71 38 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1233 1265 1465 1497 2344 2376 2440 2472 3460
Size: 32 200 32 847 32 64 32 988
Cuts at: 3460 3490 3492 3522 1233
Size: 30 2 30 3329
Fragments arranged by size:
3329 988 847 200 64 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2432 2657 2432
Size: 225 5393
HpaI GTT'AAC
Cuts at: 2432 2432
Size: 5618
KasI G'GCGC_C
Cuts at: 271 3048 3296 271
Size: 2777 248 2593
Fragments arranged by size:
2777 2593 248
KpnI G_GTAC'C
Cuts at: 1940 2638 1940
Size: 698 4920
MluI A'CGCG_T
Cuts at: 5051 5051
Size: 5618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4377 4561 4377
Size: 184 5434
MscI TGG'CCA
Cuts at: 1000 1759 2188 3379 1000
Size: 759 429 1191 3239
Fragments arranged by size:
3239 1191 759 429
MslI CAynn'nnrTG
Cuts at: 448 746 1438 2179 3607 3734 448
Size: 298 692 741 1428 127 2332
Fragments arranged by size:
2332 1428 741 692 298 127
MspA1I CmG'CkG
Cuts at: 121 142 187 247 262 391 654 1221 1603
Size: 21 45 60 15 129 263 567 382
Cuts at: 1603 1690 2211 2274 2729 3043 3403 4187 4432
Size: 87 521 63 455 314 360 784 245
Cuts at: 4432 121
Size: 1307
Fragments arranged by size:
1307 784 567 521 455 382 360 314
263 245 129 87 63 60 45 21
15
NaeI GCC'GGC
Cuts at: 2069 3799 2069
Size: 1730 3888
NarI GG'CG_CC
Cuts at: 272 3049 3297 272
Size: 2777 248 2593
Fragments arranged by size:
2777 2593 248
NcoI C'CATG_G
Cuts at: 107 1373 1433 107
Size: 1266 60 4292
Fragments arranged by size:
4292 1266 60
NgoMIV G'CCGG_C
Cuts at: 2067 3797 2067
Size: 1730 3888
NspI r_CATG'y
Cuts at: 910 2412 2671 3171 4774 910
Size: 1502 259 500 1603 1754
Fragments arranged by size:
1754 1603 1502 500 259
PasI CC'CwG_GG
Cuts at: 1393 1393
Size: 5618
PciI A'CATG_T
Cuts at: 4770 4770
Size: 5618
PflMI CCAn_nnn'nTGG
Cuts at: 1434 1954 2400 1434
Size: 520 446 4652
Fragments arranged by size:
4652 520 446
PfoI T'CCnGG_A
Cuts at: 2348 2348
Size: 5618
PmeI GTTT'AAAC
Cuts at: 2617 2617
Size: 5618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 4064
Size: 32 5586
PpuMI rG'GwC_Cy
Cuts at: 1287 1858 1873 1287
Size: 571 15 5032
Fragments arranged by size:
5032 571 15
PspOMI G'GGCC_C
Cuts at: 168 385 1229 1396 1818 168
Size: 217 844 167 422 3968
Fragments arranged by size:
3968 844 422 217 167
PstI C_TGCA'G
Cuts at: 1010 1695 1725 2078 2474 2665 3350 1010
Size: 685 30 353 396 191 685 3278
Fragments arranged by size:
3278 685 685 396 353 191 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5618
PvuII CAG'CTG
Cuts at: 1221 1603 2274 3043 3403 1221
Size: 382 671 769 360 3436
Fragments arranged by size:
3436 769 671 382 360
RsrII CG'GwC_CG
Cuts at: 3813 3813
Size: 5618
SacI G_AGCT'C
Cuts at: 2632 2632
Size: 5618
SacII CC_GC'GG
Cuts at: 122 248 263 392 122
Size: 126 15 129 5348
Fragments arranged by size:
5348 129 126 15
SalI G'TCGA_C
Cuts at: 2655 2655
Size: 5618
SanDI GG'GwC_CC
Cuts at: 1873 1873
Size: 5618
SapI GCTCTTCn'nnn_
Cuts at: 916 3641 3851 916
Size: 2725 210 2683
Fragments arranged by size:
2725 2683 210
SbfI CC_TGCA'GG
Cuts at: 1725 2078 2665 1725
Size: 353 587 4678
Fragments arranged by size:
4678 587 353
ScaI AGT'ACT
Cuts at: 5135 5135
Size: 5618
SfcI C'TryA_G
Cuts at: 33 369 1006 1691 1721 2074 2370 2470 2661
Size: 336 637 685 30 353 296 100 191
Cuts at: 2661 3346 4314 4505 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 685 685 637 353 336 296
191 191 100 30
SfoI GGC'GCC
Cuts at: 273 3050 3298 273
Size: 2777 248 2593
Fragments arranged by size:
2777 2593 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5618
SmaI CCC'GGG
Cuts at: 167 173 1961 2640 167
Size: 6 1788 679 3145
Fragments arranged by size:
3145 1788 679 6
SmlI C'TyrA_G
Cuts at: 1234 1482 2773 3688 4402 4664 1234
Size: 248 1291 915 714 262 2188
Fragments arranged by size:
2188 1291 915 714 262 248
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5618
SphI G_CATG'C
Cuts at: 2671 3171 2671
Size: 500 5118
SrfI GCCC'GGGC
Cuts at: 167 173 167
Size: 6 5612
StuI AGG'CCT
Cuts at: 1318 2503 1318
Size: 1185 4433
StyI C'CwwG_G
Cuts at: 107 668 894 929 1373 1433 2752 3106 107
Size: 561 226 35 444 60 1319 354 2619
Fragments arranged by size:
2619 1319 561 444 354 226 60 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1386 3201 3334 3793 4002 1386
Size: 1815 133 459 209 3002
Fragments arranged by size:
3002 1815 459 209 133
TatI w'GTAC_w
Cuts at: 662 2226 5133 662
Size: 1564 2907 1147
Fragments arranged by size:
2907 1564 1147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 610 642 1293 1325 5135 5167 610
Size: 32 651 32 3810 32 1061
Fragments arranged by size:
3810 1061 651 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3415 3415
Size: 5618
XbaI T'CTAG_A
Cuts at: 2649 2649
Size: 5618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 492 603 1911 2093 2295 2587 3113 492
Size: 111 1308 182 202 292 526 2997
Fragments arranged by size:
2997 1308 526 292 202 182 111
XmaI C'CCGG_G
Cuts at: 165 171 1959 2638 165
Size: 6 1788 679 3145
Fragments arranged by size:
3145 1788 679 6
XmnI GAAnn'nnTTC
Cuts at: 505 505
Size: 5618
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AgeI AlfI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BspEI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SspI
SwaI XhoI ZraI