Restriction map for
pF1KB7018
(Circular) (Six-base) MAPSORT of: pF1KB7018.seq Check: 232 from: 1 to: 6029
pF1KB7018 6029 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2790 2790
Size: 6029
AatII G_ACGT'C
Cuts at: 1304 2651 1304
Size: 1347 4682
AccI GT'mk_AC
Cuts at: 2630 2948 3067 2630
Size: 318 119 5592
Fragments arranged by size:
5592 318 119
Acc65I G'GTAC_C
Cuts at: 3045 3045
Size: 6029
AclI AA'CG_TT
Cuts at: 928 5869 928
Size: 4941 1088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3854 4286 4639 5301 3854
Size: 432 353 662 4582
Fragments arranged by size:
4582 662 432 353
AflII C'TTAA_G
Cuts at: 414 414
Size: 6029
AflIII A'CryG_T
Cuts at: 392 2633 3381 5181 5462 392
Size: 2241 748 1800 281 959
Fragments arranged by size:
2241 1800 959 748 281
AhdI GACnn_n'nnGTC
Cuts at: 2652 2652
Size: 6029
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 706 706
Size: 6029
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2006 2038 2006
Size: 32 5997
AlwNI CAG_nnn'CTG
Cuts at: 1444 2194 3003 3334 4772 1444
Size: 750 809 331 1438 2701
Fragments arranged by size:
2701 1438 809 750 331
ApaI G_GGCC'C
Cuts at: 2090 2090
Size: 6029
ApaLI G'TGCA_C
Cuts at: 396 804 3010 4867 396
Size: 408 2206 1857 1558
Fragments arranged by size:
2206 1857 1558 408
ApoI r'AATT_y
Cuts at: 703 790 2133 3033 5382 5638 703
Size: 87 1343 900 2349 256 1094
Fragments arranged by size:
2349 1343 1094 900 256 87
AseI AT'TA_AT
Cuts at: 20 1046 20
Size: 1026 5003
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6029
AvaI C'yCGr_G
Cuts at: 3049 3049
Size: 6029
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1215 1248 1215
Size: 33 5996
BamHI G'GATC_C
Cuts at: 3054 3054
Size: 6029
BanI G'GyrC_C
Cuts at: 124 557 1258 2604 3045 3459 3707 3742 124
Size: 433 701 1346 441 414 248 35 2411
Fragments arranged by size:
2411 1346 701 441 433 414 248 35
BanII G_rGCy'C
Cuts at: 1415 2090 3043 4073 1415
Size: 675 953 1030 3371
Fragments arranged by size:
3371 1030 953 675
BbeI G_GCGC'C
Cuts at: 128 3463 3711 128
Size: 3335 248 2446
Fragments arranged by size:
3335 2446 248
BbsI GAAGACnn'nnnn_
Cuts at: 497 2222 2402 2812 497
Size: 1725 180 410 3714
Fragments arranged by size:
3714 1725 410 180
BciVI GTATCCnnnnn_n'
Cuts at: 3927 4983 6029 3927
Size: 1056 1046 3927
Fragments arranged by size:
3927 1056 1046
BclI T'GATC_A
Cuts at: 620 1830 2741 3549 620
Size: 1210 911 808 3100
Fragments arranged by size:
3100 1210 911 808
BglII A'GATC_T
Cuts at: 2314 3544 5581 2314
Size: 1230 2037 2762
Fragments arranged by size:
2762 2037 1230
BlpI GC'TnA_GC
Cuts at: 2404 2764 3141 2404
Size: 360 377 5292
Fragments arranged by size:
5292 377 360
Bme1580I G_kGCm'C
Cuts at: 400 808 1983 2090 3014 3654 3747 4871 400
Size: 408 1175 107 924 640 93 1124 1558
Fragments arranged by size:
1558 1175 1124 924 640 408 107 93
BmgBI CAC'GTC
Cuts at: 803 803
Size: 6029
BmrI ACTGGGnnnn_n'
Cuts at: 3241 3322 3657 5801 3241
Size: 81 335 2144 3469
Fragments arranged by size:
3469 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 134 2700 4396 134
Size: 2566 1696 1767
Fragments arranged by size:
2566 1767 1696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 983 1907 2294 983
Size: 924 387 4718
Fragments arranged by size:
4718 924 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 606 1954 3205 4084 4798 5096 535
Size: 71 1348 1251 879 714 298 1468
Fragments arranged by size:
1468 1348 1251 879 714 298 71
BsaI GGTCTCn'nnnn_
Cuts at: 5733 5733
Size: 6029
BsaAI yAC'GTr
Cuts at: 395 1227 3382 395
Size: 832 2155 3042
Fragments arranged by size:
3042 2155 832
BsaBI GATnn'nnATC
Cuts at: 6 2822 3543 5580 6
Size: 2816 721 2037 455
Fragments arranged by size:
2816 2037 721 455
BsaHI Gr'CG_yC
Cuts at: 125 1301 2648 3460 3708 4410 5906 125
Size: 1176 1347 812 248 702 1496 248
Fragments arranged by size:
1496 1347 1176 812 702 248 248
BsaWI w'CCGG_w
Cuts at: 2756 3218 3739 4417 4828 4975 5249 2756
Size: 462 521 678 411 147 274 3536
Fragments arranged by size:
3536 678 521 462 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 670 700 1082 1112 670
Size: 30 382 30 5587
Fragments arranged by size:
5587 382 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1792 2982 1792
Size: 1190 4839
BseYI C'CCAG_C
Cuts at: 2813 2996 3454 4212 4877 5285 2813
Size: 183 458 758 665 408 3557
Fragments arranged by size:
3557 758 665 458 408 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1504 2058 2180 1504
Size: 554 122 5353
Fragments arranged by size:
5353 554 122
BsiEI CG_ry'CG
Cuts at: 104 2720 3571 3617 4847 104
Size: 2616 851 46 1230 1286
Fragments arranged by size:
2616 1286 1230 851 46
BsiHKAI G_wGCw'C
Cuts at: 400 808 2890 3014 3043 3821 4011 4871 400
Size: 408 2082 124 29 778 190 860 1558
Fragments arranged by size:
2082 1558 860 778 408 190 124 29
BsmI GAATG_Cn'
Cuts at: 1123 1552 2217 2717 1123
Size: 429 665 500 4435
Fragments arranged by size:
4435 665 500 429
BsmBI CGTCTCn'nnnn_
Cuts at: 215 215
Size: 6029
Bsp1286I G_dGCh'C
Cuts at: 400 808 1415 1983 2090 2890 3014 3043 3654
Size: 408 607 568 107 800 124 29 611
Cuts at: 3654 3747 3821 4011 4073 4871 400
Size: 93 74 190 62 798 1558
Fragments arranged by size:
1558 800 798 611 607 568 408 190
124 107 93 74 62 29
BspEI T'CCGG_A
Cuts at: 2756 2756
Size: 6029
BspHI T'CATG_A
Cuts at: 617 4461 617
Size: 3844 2185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2790 3079 3293 3595 3976 2790
Size: 289 214 302 381 4843
Fragments arranged by size:
4843 381 302 289 214
BsrBI CCG'CTC
Cuts at: 50 473 4321 4375 5859 6024 50
Size: 423 3848 54 1484 165 55
Fragments arranged by size:
3848 1484 423 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3941 3941
Size: 6029
BsrFI r'CCGG_y
Cuts at: 382 2715 4027 4208 382
Size: 2333 1312 181 2203
Fragments arranged by size:
2333 2203 1312 181
BssSI C'ACGA_G
Cuts at: 1369 4300 5008 1369
Size: 2931 708 2390
Fragments arranged by size:
2931 2390 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1703 2194 3406 1703
Size: 491 1212 4326
Fragments arranged by size:
4326 1212 491
BstBI TT'CG_AA
Cuts at: 4390 4390
Size: 6029
BstYI r'GATC_y
Cuts at: 1 314 444 1151 2314 2823 3054 3521 3544
Size: 313 130 707 1163 509 231 467 23
Cuts at: 3544 3879 4125 4431 4443 4529 5581 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1163 1052 707 509 467 449 335 313
306 246 231 130 86 23 12
BstZ17I GTA'TAC
Cuts at: 2631 2631
Size: 6029
BtgI C'CryG_G
Cuts at: 2942 2942
Size: 6029
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3979 4160 4323 3979
Size: 181 163 5685
Fragments arranged by size:
5685 181 163
BtsI GCAGTG_nn'
Cuts at: 1153 3272 1153
Size: 2119 3910
DraI TTT'AAA
Cuts at: 1632 3028 1632
Size: 1396 4633
DrdI GACnn_nn'nnGTC
Cuts at: 247 505 3735 5079 247
Size: 258 3230 1344 1197
Fragments arranged by size:
3230 1344 1197 258
EaeI y'GGCC_r
Cuts at: 1943 3614 3788 4179 4206 5551 1943
Size: 1671 174 391 27 1345 2421
Fragments arranged by size:
2421 1671 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3614 3614
Size: 6029
EarI CTCTTCn'nnn_
Cuts at: 183 578 4052 4262 183
Size: 395 3474 210 1950
Fragments arranged by size:
3474 1950 395 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4237 4979 5125 5839 4237
Size: 742 146 714 4427
Fragments arranged by size:
4427 742 714 146
EcoICRI GAG'CTC
Cuts at: 3041 3041
Size: 6029
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 983 1907 2294 3854 4286 4639 5301 983
Size: 924 387 1560 432 353 662 1711
Fragments arranged by size:
1711 1560 924 662 432 387 353
EcoO109I rG'GnC_Cy
Cuts at: 2086 3168 2086
Size: 1082 4947
EcoRI G'AATT_C
Cuts at: 790 3033 790
Size: 2243 3786
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2769 4048 2215
Size: 554 1279 4196
Fragments arranged by size:
4196 1279 554
FspI TGC'GCA
Cuts at: 3810 3810
Size: 6029
HaeII r_GCGC'y
Cuts at: 119 128 3463 3711 4941 5712 119
Size: 9 3335 248 1230 771 436
Fragments arranged by size:
3335 1230 771 436 248 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 670 702 2638 2670 2877 2909 3871 3901 3903
Size: 32 1936 32 207 32 962 30 2
Cuts at: 3903 3933 670
Size: 30 2766
Fragments arranged by size:
2766 1936 962 207 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 468 932 3068 468
Size: 464 2136 3429
Fragments arranged by size:
3429 2136 464
HindIII A'AGCT_T
Cuts at: 454 454
Size: 6029
KasI G'GCGC_C
Cuts at: 124 3459 3707 124
Size: 3335 248 2446
Fragments arranged by size:
3335 2446 248
KpnI G_GTAC'C
Cuts at: 3049 3049
Size: 6029
MluI A'CGCG_T
Cuts at: 5462 5462
Size: 6029
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 677 2485 2930 4788 4972 677
Size: 1808 445 1858 184 1734
Fragments arranged by size:
1858 1808 1734 445 184
MscI TGG'CCA
Cuts at: 3790 3790
Size: 6029
MslI CAynn'nnrTG
Cuts at: 430 899 1711 2263 3017 4018 4145 430
Size: 469 812 552 754 1001 127 2314
Fragments arranged by size:
2314 1001 812 754 552 469 127
MspA1I CmG'CkG
Cuts at: 512 3140 3454 3814 4598 4843 512
Size: 2628 314 360 784 245 1698
Fragments arranged by size:
2628 1698 784 360 314 245
NaeI GCC'GGC
Cuts at: 4210 4210
Size: 6029
NarI GG'CG_CC
Cuts at: 125 3460 3708 125
Size: 3335 248 2446
Fragments arranged by size:
3335 2446 248
NgoMIV G'CCGG_C
Cuts at: 4208 4208
Size: 6029
NspI r_CATG'y
Cuts at: 1383 3082 3582 5185 1383
Size: 1699 500 1603 2227
Fragments arranged by size:
2227 1699 1603 500
PacI TTA_AT'TAA
Cuts at: 1931 1931
Size: 6029
PasI CC'CwG_GG
Cuts at: 1878 1878
Size: 6029
PciI A'CATG_T
Cuts at: 5181 5181
Size: 6029
PflMI CCAn_nnn'nTGG
Cuts at: 1392 1513 1392
Size: 121 5908
PfoI T'CCnGG_A
Cuts at: 228 317 1437 2706 228
Size: 89 1120 1269 3551
Fragments arranged by size:
3551 1269 1120 89
PmeI GTTT'AAAC
Cuts at: 3028 3028
Size: 6029
PmlI CAC'GTG
Cuts at: 395 395
Size: 6029
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 4475
Size: 32 5997
PsiI TTA'TAA
Cuts at: 2204 2204
Size: 6029
PspOMI G'GGCC_C
Cuts at: 2086 2086
Size: 6029
PstI C_TGCA'G
Cuts at: 2239 2923 3076 3761 2239
Size: 684 153 685 4507
Fragments arranged by size:
4507 685 684 153
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6029
PvuII CAG'CTG
Cuts at: 3454 3814 3454
Size: 360 5669
RsrII CG'GwC_CG
Cuts at: 4224 4224
Size: 6029
SacI G_AGCT'C
Cuts at: 3043 3043
Size: 6029
SalI G'TCGA_C
Cuts at: 3066 3066
Size: 6029
SapI GCTCTTCn'nnn_
Cuts at: 183 4052 4262 183
Size: 3869 210 1950
Fragments arranged by size:
3869 1950 210
SbfI CC_TGCA'GG
Cuts at: 3076 3076
Size: 6029
ScaI AGT'ACT
Cuts at: 2247 5546 2247
Size: 3299 2730
SexAI A'CCwGG_T
Cuts at: 2417 2417
Size: 6029
SfcI C'TryA_G
Cuts at: 33 871 1070 1538 2235 2919 3072 3757 4725
Size: 838 199 468 697 684 153 685 968
Cuts at: 4725 4916 33
Size: 191 1146
Fragments arranged by size:
1146 968 838 697 685 684 468 199
191 153
SfoI GGC'GCC
Cuts at: 126 3461 3709 126
Size: 3335 248 2446
Fragments arranged by size:
3335 2446 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6029
SmaI CCC'GGG
Cuts at: 3051 3051
Size: 6029
SmlI C'TyrA_G
Cuts at: 414 550 585 1969 3184 4099 4813 5075 414
Size: 136 35 1384 1215 915 714 262 1368
Fragments arranged by size:
1384 1368 1215 915 714 262 136 35
SnaBI TAC'GTA
Cuts at: 1227 1227
Size: 6029
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6029
SphI G_CATG'C
Cuts at: 1383 3082 3582 1383
Size: 1699 500 3830
Fragments arranged by size:
3830 1699 500
SspI AAT'ATT
Cuts at: 1735 2052 2110 1735
Size: 317 58 5654
Fragments arranged by size:
5654 317 58
StyI C'CwwG_G
Cuts at: 578 605 1019 2034 2297 3163 3517 578
Size: 27 414 1015 263 866 354 3090
Fragments arranged by size:
3090 1015 866 414 354 263 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1870 3612 3745 4204 4413 1870
Size: 1742 133 459 209 3486
Fragments arranged by size:
3486 1742 459 209 133
TatI w'GTAC_w
Cuts at: 833 1467 1758 2245 2255 5544 833
Size: 634 291 487 10 3289 1318
Fragments arranged by size:
3289 1318 634 487 291 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 206 238 1508 1540 5546 5578 206
Size: 32 1270 32 4006 32 657
Fragments arranged by size:
4006 1270 657 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3019 3826 3019
Size: 807 5222
XbaI T'CTAG_A
Cuts at: 3060 3060
Size: 6029
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3524 3524
Size: 6029
XmaI C'CCGG_G
Cuts at: 3049 3049
Size: 6029
ZraI GAC'GTC
Cuts at: 1302 2649 1302
Size: 1347 4682
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AgeI AleI AscI AvrII BbvCI BcgI BfrBI
BglI BmtI Bpu10I BsiWI BsrGI BssHII BstEII BstXI
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PpuMI PshAI PspXI PsrI SacII SanDI SfiI SgrAI
SrfI StuI SwaI XhoI XmnI