Restriction map for
pF1KB7020
(Circular) (Six-base) MAPSORT of: pF1KB7020.seq Check: 3448 from: 1 to: 6107
pF1KB7020 6107 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 835 1072 835
Size: 237 5870
AccI GT'mk_AC
Cuts at: 422 2058 3145 422
Size: 1636 1087 3384
Fragments arranged by size:
3384 1636 1087
Acc65I G'GTAC_C
Cuts at: 3123 3123
Size: 6107
AclI AA'CG_TT
Cuts at: 5947 5947
Size: 6107
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1490 2128 3932 4364 4717 5379 1490
Size: 638 1804 432 353 662 2218
Fragments arranged by size:
2218 1804 662 638 432 353
AfeI AGC'GCT
Cuts at: 2829 2829
Size: 6107
AflIII A'CryG_T
Cuts at: 1994 3459 5259 5540 1994
Size: 1465 1800 281 2561
Fragments arranged by size:
2561 1800 1465 281
AleI CACnn'nnGTG
Cuts at: 637 1120 637
Size: 483 5624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1138 1925 1138
Size: 787 5320
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 421 453 421
Size: 32 6075
AlwNI CAG_nnn'CTG
Cuts at: 1058 1112 1693 3087 3412 4850 1058
Size: 54 581 1394 325 1438 2315
Fragments arranged by size:
2315 1438 1394 581 325 54
ApaI G_GGCC'C
Cuts at: 1007 1758 1007
Size: 751 5356
ApaLI G'TGCA_C
Cuts at: 2371 4945 2371
Size: 2574 3533
ApoI r'AATT_y
Cuts at: 2035 2758 3111 5460 5716 2035
Size: 723 353 2349 256 2426
Fragments arranged by size:
2426 2349 723 353 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6107
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6107
AvaI C'yCGr_G
Cuts at: 775 1136 1177 1193 1401 2197 3127 775
Size: 361 41 16 208 796 930 3755
Fragments arranged by size:
3755 930 796 361 208 41 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1017 1050 1017
Size: 33 6074
BamHI G'GATC_C
Cuts at: 3132 3132
Size: 6107
BanI G'GyrC_C
Cuts at: 697 823 855 897 907 1527 3123 3537 3785
Size: 126 32 42 10 620 1596 414 248
Cuts at: 3785 3820 697
Size: 35 2984
Fragments arranged by size:
2984 1596 620 414 248 126 42 35
32 10
BanII G_rGCy'C
Cuts at: 120 434 800 1007 1193 1414 1758 1795 3121
Size: 314 366 207 186 221 344 37 1326
Cuts at: 3121 4151 120
Size: 1030 2076
Fragments arranged by size:
2076 1326 1030 366 344 314 221 207
186 37
BbeI G_GCGC'C
Cuts at: 701 3541 3789 701
Size: 2840 248 3019
Fragments arranged by size:
3019 2840 248
BbsI GAAGACnn'nnnn_
Cuts at: 1806 3002 1806
Size: 1196 4911
BbvCI CC'TCA_GC
Cuts at: 534 1266 534
Size: 732 5375
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2368 2402 2368
Size: 34 6073
BciVI GTATCCnnnnn_n'
Cuts at: 3064 4005 5061 6107 3064
Size: 941 1056 1046 3064
Fragments arranged by size:
3064 1056 1046 941
BclI T'GATC_A
Cuts at: 2175 3627 2175
Size: 1452 4655
BglI GCCn_nnn'nGGC
Cuts at: 150 2530 150
Size: 2380 3727
BglII A'GATC_T
Cuts at: 3622 5659 3622
Size: 2037 4070
BlpI GC'TnA_GC
Cuts at: 3219 3219
Size: 6107
Bme1580I G_kGCm'C
Cuts at: 1007 1478 1530 1683 1758 2375 3732 3825 4949
Size: 471 52 153 75 617 1357 93 1124
Cuts at: 4949 1007
Size: 2165
Fragments arranged by size:
2165 1357 1124 617 471 153 93 75
52
BmgBI CAC'GTC
Cuts at: 1995 1995
Size: 6107
BmrI ACTGGGnnnn_n'
Cuts at: 797 948 2634 3319 3400 3735 5879 797
Size: 151 1686 685 81 335 2144 1025
Fragments arranged by size:
2144 1686 1025 685 335 151 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 621 819 1764 4474 621
Size: 198 945 2710 2254
Fragments arranged by size:
2710 2254 945 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 1750 2476 2592 1277
Size: 473 726 116 4792
Fragments arranged by size:
4792 726 473 116
Bpu10I CC'TnA_GC
Cuts at: 534 1266 1500 2146 3046 534
Size: 732 234 646 900 3595
Fragments arranged by size:
3595 900 732 646 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 847 2797 3283 4162 4876 5174 418
Size: 429 1950 486 879 714 298 1351
Fragments arranged by size:
1950 1351 879 714 486 429 298
BsaI GGTCTCn'nnnn_
Cuts at: 220 484 772 1723 5811 220
Size: 264 288 951 4088 516
Fragments arranged by size:
4088 951 516 288 264
BsaAI yAC'GTr
Cuts at: 426 570 3460 426
Size: 144 2890 3073
Fragments arranged by size:
3073 2890 144
BsaBI GATnn'nnATC
Cuts at: 6 2509 3621 5658 6
Size: 2503 1112 2037 455
Fragments arranged by size:
2503 2037 1112 455
BsaHI Gr'CG_yC
Cuts at: 698 1655 3538 3786 4488 5984 698
Size: 957 1883 248 702 1496 821
Fragments arranged by size:
1883 1496 957 821 702 248
BsaWI w'CCGG_w
Cuts at: 2741 3296 3817 4495 4906 5053 5327 2741
Size: 555 521 678 411 147 274 3521
Fragments arranged by size:
3521 678 555 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 421 451 940 970 2837 2867 421
Size: 30 489 30 1867 30 3661
Fragments arranged by size:
3661 1867 489 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 531 2029 531
Size: 1498 4609
BseYI C'CCAG_C
Cuts at: 1044 1314 1757 1804 2029 2729 3532 4290 4955
Size: 270 443 47 225 700 803 758 665
Cuts at: 4955 5363 1044
Size: 408 1788
Fragments arranged by size:
1788 803 758 700 665 443 408 270
225 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 897 1044 1098 1106 1402 1474 1651 2692
Size: 464 147 54 8 296 72 177 1041
Cuts at: 2692 3115 433
Size: 423 3425
Fragments arranged by size:
3425 1041 464 423 296 177 147 72
54 8
BsiEI CG_ry'CG
Cuts at: 104 624 1436 3649 3695 4925 104
Size: 520 812 2213 46 1230 1286
Fragments arranged by size:
2213 1286 1230 812 520 46
BsiHKAI G_wGCw'C
Cuts at: 434 2375 3121 3899 4089 4949 434
Size: 1941 746 778 190 860 1592
Fragments arranged by size:
1941 1592 860 778 746 190
Bsp1286I G_dGCh'C
Cuts at: 120 434 800 1007 1193 1414 1478 1530 1683
Size: 314 366 207 186 221 64 52 153
Cuts at: 1683 1758 1795 2375 3121 3732 3825 3899 4089
Size: 75 37 580 746 611 93 74 190
Cuts at: 4089 4151 4949 120
Size: 62 798 1278
Fragments arranged by size:
1278 798 746 611 580 366 314 221
207 190 186 153 93 75 74 64
62 52 37
BspHI T'CATG_A
Cuts at: 2600 4539 2600
Size: 1939 4168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 835 1072 3008 3087 3157 3371 3673 4054 835
Size: 237 1936 79 70 214 302 381 2888
Fragments arranged by size:
2888 1936 381 302 237 214 79 70
BsrBI CCG'CTC
Cuts at: 50 1539 4399 4453 5937 6102 50
Size: 1489 2860 54 1484 165 55
Fragments arranged by size:
2860 1489 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 584 812 1907 2330 2805 2819 4019 584
Size: 228 1095 423 475 14 1200 2672
Fragments arranged by size:
2672 1200 1095 475 423 228 14
BsrFI r'CCGG_y
Cuts at: 205 619 925 2338 2672 3025 4105 4286 205
Size: 414 306 1413 334 353 1080 181 2026
Fragments arranged by size:
2026 1413 1080 414 353 334 306 181
BsrGI T'GTAC_A
Cuts at: 1223 1223
Size: 6107
BssHII G'CGCG_C
Cuts at: 723 723
Size: 6107
BssSI C'ACGA_G
Cuts at: 4378 5086 4378
Size: 708 5399
BstAPI GCAn_nnn'nTGC
Cuts at: 1244 3484 1244
Size: 2240 3867
BstBI TT'CG_AA
Cuts at: 4468 4468
Size: 6107
BstEII G'GTnAC_C
Cuts at: 2208 3015 2208
Size: 807 5300
BstXI CCAn_nnnn'nTGG
Cuts at: 807 1255 2493 2922 807
Size: 448 1238 429 3992
Fragments arranged by size:
3992 1238 448 429
BstYI r'GATC_y
Cuts at: 1 2504 3041 3132 3599 3622 3957 4203 4509
Size: 2503 537 91 467 23 335 246 306
Cuts at: 4509 4521 4607 5659 1
Size: 12 86 1052 449
Fragments arranged by size:
2503 1052 537 467 449 335 306 246
91 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1852 2749 1852
Size: 897 5210
BtgI C'CryG_G
Cuts at: 107 842 1248 2052 2657 2687 107
Size: 735 406 804 605 30 3527
Fragments arranged by size:
3527 804 735 605 406 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 539 896 1581 1821 4057 4238 4401 539
Size: 357 685 240 2236 181 163 2245
Fragments arranged by size:
2245 2236 685 357 240 181 163
BtsI GCAGTG_nn'
Cuts at: 2704 3350 2704
Size: 646 5461
DraI TTT'AAA
Cuts at: 3106 3106
Size: 6107
DraIII CAC_nnn'GTG
Cuts at: 1282 1282
Size: 6107
DrdI GACnn_nn'nnGTC
Cuts at: 2866 3813 5157 2866
Size: 947 1344 3816
Fragments arranged by size:
3816 1344 947
EaeI y'GGCC_r
Cuts at: 110 621 927 1206 1433 2073 2093 2221 3023
Size: 511 306 279 227 640 20 128 802
Cuts at: 3023 3027 3692 3866 4257 4284 5629 110
Size: 4 665 174 391 27 1345 588
Fragments arranged by size:
1345 802 665 640 588 511 391 306
279 227 174 128 27 20 4
EagI C'GGCC_G
Cuts at: 621 1433 3692 621
Size: 812 2259 3036
Fragments arranged by size:
3036 2259 812
EarI CTCTTCn'nnn_
Cuts at: 218 290 508 775 1579 4130 4340 218
Size: 72 218 267 804 2551 210 1985
Fragments arranged by size:
2551 1985 804 267 218 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 808 4315 5057 5203 5917 808
Size: 3507 742 146 714 998
Fragments arranged by size:
3507 998 742 714 146
EcoICRI GAG'CTC
Cuts at: 432 3119 432
Size: 2687 3420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 1490 1750 2128 2476 2592 3932 4364 4717
Size: 213 260 378 348 116 1340 432 353
Cuts at: 4717 5379 1277
Size: 662 2005
Fragments arranged by size:
2005 1340 662 432 378 353 348 260
213 116
EcoNI CCTnn'n_nnAGG
Cuts at: 2149 2149
Size: 6107
EcoO109I rG'GnC_Cy
Cuts at: 1003 1004 1197 1262 1754 1953 3246 1003
Size: 1 193 65 492 199 1293 3864
Fragments arranged by size:
3864 1293 492 199 193 65 1
EcoRI G'AATT_C
Cuts at: 3111 3111
Size: 6107
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 510 687 2958 4126 510
Size: 177 2271 1168 2491
Fragments arranged by size:
2491 2271 1168 177
FseI GG_CCGG'CC
Cuts at: 3029 3029
Size: 6107
FspI TGC'GCA
Cuts at: 643 3888 643
Size: 3245 2862
HaeII r_GCGC'y
Cuts at: 220 701 1332 2831 3541 3789 5019 5790 220
Size: 481 631 1499 710 248 1230 771 537
Fragments arranged by size:
1499 1230 771 710 631 537 481 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 261 293 517 549 1081 1113 1130 1162 2452
Size: 32 224 32 532 32 17 32 1290
Cuts at: 2452 2484 3949 3979 3981 4011 261
Size: 32 1465 30 2 30 2357
Fragments arranged by size:
2357 1465 1290 532 224 32 32 32
32 32 30 30 17 2
HincII GTy'rAC
Cuts at: 630 1392 2059 2406 3146 630
Size: 762 667 347 740 3591
Fragments arranged by size:
3591 762 740 667 347
KasI G'GCGC_C
Cuts at: 697 3537 3785 697
Size: 2840 248 3019
Fragments arranged by size:
3019 2840 248
KpnI G_GTAC'C
Cuts at: 3127 3127
Size: 6107
MluI A'CGCG_T
Cuts at: 5540 5540
Size: 6107
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4866 5050 4866
Size: 184 5923
MscI TGG'CCA
Cuts at: 112 2095 3868 112
Size: 1983 1773 2351
Fragments arranged by size:
2351 1983 1773
MslI CAynn'nnrTG
Cuts at: 637 718 1120 2920 4096 4223 637
Size: 81 402 1800 1176 127 2521
Fragments arranged by size:
2521 1800 1176 402 127 81
MspA1I CmG'CkG
Cuts at: 162 174 189 417 940 1314 1635 1804 2133
Size: 12 15 228 523 374 321 169 329
Cuts at: 2133 2310 2516 3218 3532 3892 4676 4921 162
Size: 177 206 702 314 360 784 245 1348
Fragments arranged by size:
1348 784 702 523 374 360 329 321
314 245 228 206 177 169 15 12
NaeI GCC'GGC
Cuts at: 207 621 2340 3027 4288 207
Size: 414 1719 687 1261 2026
Fragments arranged by size:
2026 1719 1261 687 414
NarI GG'CG_CC
Cuts at: 698 3538 3786 698
Size: 2840 248 3019
Fragments arranged by size:
3019 2840 248
NcoI C'CATG_G
Cuts at: 107 1248 2687 107
Size: 1141 1439 3527
Fragments arranged by size:
3527 1439 1141
NgoMIV G'CCGG_C
Cuts at: 205 619 2338 3025 4286 205
Size: 414 1719 687 1261 2026
Fragments arranged by size:
2026 1719 1261 687 414
NspI r_CATG'y
Cuts at: 708 2322 2844 3066 3160 3660 5263 708
Size: 1614 522 222 94 500 1603 1552
Fragments arranged by size:
1614 1603 1552 522 500 222 94
PasI CC'CwG_GG
Cuts at: 973 2493 973
Size: 1520 4587
PciI A'CATG_T
Cuts at: 5259 5259
Size: 6107
PflMI CCAn_nnn'nTGG
Cuts at: 286 1353 2313 2516 286
Size: 1067 960 203 3877
Fragments arranged by size:
3877 1067 960 203
PfoI T'CCnGG_A
Cuts at: 686 1556 1766 1914 2453 2855 3056 686
Size: 870 210 148 539 402 201 3737
Fragments arranged by size:
3737 870 539 402 210 201 148
PmeI GTTT'AAAC
Cuts at: 3106 3106
Size: 6107
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 421 453 4553 4585 421
Size: 32 4100 32 1943
Fragments arranged by size:
4100 1943 32 32
PpuMI rG'GwC_Cy
Cuts at: 1197 1953 1197
Size: 756 5351
PspOMI G'GGCC_C
Cuts at: 1003 1754 1003
Size: 751 5356
PspXI vC'TCGA_Gb
Cuts at: 1177 2197 1177
Size: 1020 5087
PstI C_TGCA'G
Cuts at: 1901 3074 3154 3839 1901
Size: 1173 80 685 4169
Fragments arranged by size:
4169 1173 685 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6107
PvuII CAG'CTG
Cuts at: 940 1314 1635 1804 2310 3532 3892 940
Size: 374 321 169 506 1222 360 3155
Fragments arranged by size:
3155 1222 506 374 360 321 169
RsrII CG'GwC_CG
Cuts at: 4302 4302
Size: 6107
SacI G_AGCT'C
Cuts at: 434 3121 434
Size: 2687 3420
SalI G'TCGA_C
Cuts at: 2057 3144 2057
Size: 1087 5020
SapI GCTCTTCn'nnn_
Cuts at: 1579 4130 4340 1579
Size: 2551 210 3346
Fragments arranged by size:
3346 2551 210
SbfI CC_TGCA'GG
Cuts at: 3154 3154
Size: 6107
ScaI AGT'ACT
Cuts at: 1576 5624 1576
Size: 4048 2059
SexAI A'CCwGG_T
Cuts at: 341 2414 2972 341
Size: 2073 558 3476
Fragments arranged by size:
3476 2073 558
SfcI C'TryA_G
Cuts at: 33 1799 1897 3070 3150 3835 4803 4994 33
Size: 1766 98 1173 80 685 968 191 1146
Fragments arranged by size:
1766 1173 1146 968 685 191 98 80
SfoI GGC'GCC
Cuts at: 699 3539 3787 699
Size: 2840 248 3019
Fragments arranged by size:
3019 2840 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6107
SmaI CCC'GGG
Cuts at: 1195 3129 1195
Size: 1934 4173
SmlI C'TyrA_G
Cuts at: 433 862 1177 2197 2812 3262 4177 4891 5153
Size: 429 315 1020 615 450 915 714 262
Cuts at: 5153 433
Size: 1387
Fragments arranged by size:
1387 1020 915 714 615 450 429 315
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6107
SphI G_CATG'C
Cuts at: 3160 3660 3160
Size: 500 5607
StuI AGG'CCT
Cuts at: 2155 2155
Size: 6107
StyI C'CwwG_G
Cuts at: 107 635 662 944 1248 1982 2687 3241 3595
Size: 528 27 282 304 734 705 554 354
Cuts at: 3595 107
Size: 2619
Fragments arranged by size:
2619 734 705 554 528 354 304 282
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 278 895 1192 2700 2721 3690 3823 4282 4491
Size: 617 297 1508 21 969 133 459 209
Cuts at: 4491 278
Size: 1894
Fragments arranged by size:
1894 1508 969 617 459 297 209 133
21
TatI w'GTAC_w
Cuts at: 1223 1574 1706 1883 1904 5622 1223
Size: 351 132 177 21 3718 1708
Fragments arranged by size:
3718 1708 351 177 132 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 940 972 2837 2869 5624 5656 940
Size: 32 1865 32 2755 32 1391
Fragments arranged by size:
2755 1865 1391 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3904 3904
Size: 6107
XbaI T'CTAG_A
Cuts at: 3138 3138
Size: 6107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 287 3602 287
Size: 3315 2792
XhoI C'TCGA_G
Cuts at: 1177 2197 1177
Size: 1020 5087
XmaI C'CCGG_G
Cuts at: 1193 3127 1193
Size: 1934 4173
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AscI AvrII BfrBI BmtI
BsiWI BsmI BsmBI BspEI BstZ17I ClaI CspCI EcoRV
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI