Restriction map for
pF1KB7022
(Circular) (Six-base) MAPSORT of: pF1KB7022.seq Check: 2434 from: 1 to: 6029
pF1KB7022 6029 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AatII G_ACGT'C
Cuts at: 2621 2621
Size: 6029
AccI GT'mk_AC
Cuts at: 2198 2600 3067 2198
Size: 402 467 5160
Fragments arranged by size:
5160 467 402
Acc65I G'GTAC_C
Cuts at: 1376 3045 1376
Size: 1669 4360
AclI AA'CG_TT
Cuts at: 5869 5869
Size: 6029
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 1960 1981 2029 2821 3854 4286 4639 5301
Size: 720 21 48 792 1033 432 353 662
Cuts at: 5301 1240
Size: 1968
Fragments arranged by size:
1968 1033 792 720 662 432 353 48
21
AflIII A'CryG_T
Cuts at: 3381 5181 5462 3381
Size: 1800 281 3948
Fragments arranged by size:
3948 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 2471 2622 2471
Size: 151 5878
AleI CACnn'nnGTG
Cuts at: 1657 2275 1657
Size: 618 5411
AlwNI CAG_nnn'CTG
Cuts at: 1518 3334 4772 1518
Size: 1816 1438 2775
Fragments arranged by size:
2775 1816 1438
ApaI G_GGCC'C
Cuts at: 115 713 1569 115
Size: 598 856 4575
Fragments arranged by size:
4575 856 598
ApaLI G'TGCA_C
Cuts at: 4867 4867
Size: 6029
ApoI r'AATT_y
Cuts at: 3033 5382 5638 3033
Size: 2349 256 3424
Fragments arranged by size:
3424 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6029
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6029
AvaI C'yCGr_G
Cuts at: 2185 2218 3049 2185
Size: 33 831 5165
Fragments arranged by size:
5165 831 33
BamHI G'GATC_C
Cuts at: 3054 3054
Size: 6029
BanI G'GyrC_C
Cuts at: 729 1351 1376 3045 3459 3707 3742 729
Size: 622 25 1669 414 248 35 3016
Fragments arranged by size:
3016 1669 622 414 248 35 25
BanII G_rGCy'C
Cuts at: 115 182 713 1021 1064 1154 1281 1295 1493
Size: 67 531 308 43 90 127 14 198
Cuts at: 1493 1569 1623 2173 2363 2798 2947 3043 4073
Size: 76 54 550 190 435 149 96 1030
Cuts at: 4073 115
Size: 2071
Fragments arranged by size:
2071 1030 550 531 435 308 198 190
149 127 96 90 76 67 54 43
14
BbeI G_GCGC'C
Cuts at: 3463 3711 3463
Size: 248 5781
BbsI GAAGACnn'nnnn_
Cuts at: 804 804
Size: 6029
BbvCI CC'TCA_GC
Cuts at: 313 2144 313
Size: 1831 4198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 503 537 503
Size: 34 5995
BciVI GTATCCnnnnn_n'
Cuts at: 2716 3927 4983 6029 2716
Size: 1211 1056 1046 2716
Fragments arranged by size:
2716 1211 1056 1046
BclI T'GATC_A
Cuts at: 3549 3549
Size: 6029
BglI GCCn_nnn'nGGC
Cuts at: 2027 2785 2027
Size: 758 5271
BglII A'GATC_T
Cuts at: 2648 2822 3544 5581 2648
Size: 174 722 2037 3096
Fragments arranged by size:
3096 2037 722 174
BlpI GC'TnA_GC
Cuts at: 780 3141 780
Size: 2361 3668
Bme1580I G_kGCm'C
Cuts at: 115 645 713 1569 3654 3747 4871 115
Size: 530 68 856 2085 93 1124 1273
Fragments arranged by size:
2085 1273 1124 856 530 93 68
BmgBI CAC'GTC
Cuts at: 2442 2442
Size: 6029
BmrI ACTGGGnnnn_n'
Cuts at: 163 176 3241 3322 3657 5801 163
Size: 13 3065 81 335 2144 391
Fragments arranged by size:
3065 2144 391 335 81 13
BmtI G_CTAG'C
Cuts at: 442 442
Size: 6029
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2670 2961 4396 2670
Size: 291 1435 4303
Fragments arranged by size:
4303 1435 291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 389 1293 1898 2238 2367 2377 2953 285
Size: 104 904 605 340 129 10 576 3361
Fragments arranged by size:
3361 904 605 576 340 129 104 10
Bpu10I CC'TnA_GC
Cuts at: 191 313 1148 1480 2144 2167 191
Size: 122 835 332 664 23 4053
Fragments arranged by size:
4053 835 664 332 122 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1035 3205 4084 4798 5096 1035
Size: 2170 879 714 298 1968
Fragments arranged by size:
2170 1968 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 365 1525 5733 365
Size: 1160 4208 661
Fragments arranged by size:
4208 1160 661
BsaAI yAC'GTr
Cuts at: 3382 3382
Size: 6029
BsaBI GATnn'nnATC
Cuts at: 6 670 3543 5580 6
Size: 664 2873 2037 455
Fragments arranged by size:
2873 2037 664 455
BsaHI Gr'CG_yC
Cuts at: 518 2195 2618 3460 3708 4410 5906 518
Size: 1677 423 842 248 702 1496 641
Fragments arranged by size:
1677 1496 842 702 641 423 248
BsaWI w'CCGG_w
Cuts at: 3218 3739 4417 4828 4975 5249 3218
Size: 521 678 411 147 274 3998
Fragments arranged by size:
3998 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 203 233 958 988 2066 2096 203
Size: 30 725 30 1078 30 4136
Fragments arranged by size:
4136 1078 725 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2629 2950 2953 2629
Size: 321 3 5705
Fragments arranged by size:
5705 321 3
BseYI C'CCAG_C
Cuts at: 714 1382 2154 2161 2735 2783 2987 3454 4212
Size: 668 772 7 574 48 204 467 758
Cuts at: 4212 4877 5285 714
Size: 665 408 1458
Fragments arranged by size:
1458 772 758 668 665 574 467 408
204 48 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 342 712 976 1428 1531 1581 342
Size: 370 264 452 103 50 4790
Fragments arranged by size:
4790 452 370 264 103 50
BsiEI CG_ry'CG
Cuts at: 104 3571 3617 4847 104
Size: 3467 46 1230 1286
Fragments arranged by size:
3467 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 781 1021 1281 1295 1544 2363 2947 2957 3043
Size: 240 260 14 249 819 584 10 86
Cuts at: 3043 3821 4011 4871 781
Size: 778 190 860 1939
Fragments arranged by size:
1939 860 819 778 584 260 249 240
190 86 14 10
BsmBI CGTCTCn'nnnn_
Cuts at: 525 525
Size: 6029
Bsp1286I G_dGCh'C
Cuts at: 115 182 645 713 781 1021 1064 1154 1281
Size: 67 463 68 68 240 43 90 127
Cuts at: 1281 1295 1493 1544 1569 1623 2173 2363 2798
Size: 14 198 51 25 54 550 190 435
Cuts at: 2798 2947 2957 3043 3654 3747 3821 4011 4073
Size: 149 10 86 611 93 74 190 62
Cuts at: 4073 4871 115
Size: 798 1273
Fragments arranged by size:
1273 798 611 550 463 435 240 198
190 190 149 127 93 90 86 74
68 68 67 62 54 51 43 25
14 10
BspHI T'CATG_A
Cuts at: 2015 2516 4461 2015
Size: 501 1945 3583
Fragments arranged by size:
3583 1945 501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1440 1859 2126 2378 2971 3079 3293 3595 3976
Size: 419 267 252 593 108 214 302 381
Cuts at: 3976 1440
Size: 3493
Fragments arranged by size:
3493 593 419 381 302 267 252 214
108
BsrBI CCG'CTC
Cuts at: 50 2868 4321 4375 5859 6024 50
Size: 2818 1453 54 1484 165 55
Fragments arranged by size:
2818 1484 1453 165 55 54
BsrDI GCAATG_nn'
Cuts at: 246 2553 3941 246
Size: 2307 1388 2334
Fragments arranged by size:
2334 2307 1388
BsrFI r'CCGG_y
Cuts at: 542 925 4027 4208 542
Size: 383 3102 181 2363
Fragments arranged by size:
3102 2363 383 181
BsrGI T'GTAC_A
Cuts at: 1718 1718
Size: 6029
BssSI C'ACGA_G
Cuts at: 2035 4300 5008 2035
Size: 2265 708 3056
Fragments arranged by size:
3056 2265 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1518 3406 1518
Size: 1888 4141
BstBI TT'CG_AA
Cuts at: 4390 4390
Size: 6029
BstEII G'GTnAC_C
Cuts at: 219 1044 219
Size: 825 5204
BstXI CCAn_nnnn'nTGG
Cuts at: 1271 1271
Size: 6029
BstYI r'GATC_y
Cuts at: 1 2648 2822 3054 3521 3544 3879 4125 4431
Size: 2647 174 232 467 23 335 246 306
Cuts at: 4431 4443 4529 5581 1
Size: 12 86 1052 449
Fragments arranged by size:
2647 1052 467 449 335 306 246 232
174 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1472 1472
Size: 6029
BtgI C'CryG_G
Cuts at: 107 1049 1904 1967 2070 107
Size: 942 855 63 103 4066
Fragments arranged by size:
4066 942 855 103 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 243 3979 4160 4323 243
Size: 3736 181 163 1949
Fragments arranged by size:
3736 1949 181 163
BtsI GCAGTG_nn'
Cuts at: 645 1411 1416 2931 3272 645
Size: 766 5 1515 341 3402
Fragments arranged by size:
3402 1515 766 341 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1893 1928 1893
Size: 35 5994
DraI TTT'AAA
Cuts at: 3028 3028
Size: 6029
DraIII CAC_nnn'GTG
Cuts at: 1503 1503
Size: 6029
DrdI GACnn_nn'nnGTC
Cuts at: 3735 5079 3735
Size: 1344 4685
EaeI y'GGCC_r
Cuts at: 140 540 944 1236 1253 1413 2175 2480 3614
Size: 400 404 292 17 160 762 305 1134
Cuts at: 3614 3788 4179 4206 5551 140
Size: 174 391 27 1345 618
Fragments arranged by size:
1345 1134 762 618 404 400 391 305
292 174 160 27 17
EagI C'GGCC_G
Cuts at: 3614 3614
Size: 6029
EarI CTCTTCn'nnn_
Cuts at: 1654 4052 4262 1654
Size: 2398 210 3421
Fragments arranged by size:
3421 2398 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4237 4979 5125 5839 4237
Size: 742 146 714 4427
Fragments arranged by size:
4427 742 714 146
EcoICRI GAG'CTC
Cuts at: 1019 1279 1293 2361 2945 3041 1019
Size: 260 14 1068 584 96 4007
Fragments arranged by size:
4007 1068 584 260 96 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 389 1240 1293 1898 1960 1981 2029 2238
Size: 104 851 53 605 62 21 48 209
Cuts at: 2238 2367 2377 2821 2953 3854 4286 4639 5301
Size: 129 10 444 132 901 432 353 662
Cuts at: 5301 285
Size: 1013
Fragments arranged by size:
1013 901 851 662 605 444 432 353
209 132 129 104 62 53 48 21
10
EcoNI CCTnn'n_nnAGG
Cuts at: 1036 1483 2335 1036
Size: 447 852 4730
Fragments arranged by size:
4730 852 447
EcoO109I rG'GnC_Cy
Cuts at: 347 709 710 1126 1476 1565 1992 2077 2158
Size: 362 1 416 350 89 427 85 81
Cuts at: 2158 2181 2367 3168 347
Size: 23 186 801 3208
Fragments arranged by size:
3208 801 427 416 362 350 186 89
85 81 23 1
EcoRI G'AATT_C
Cuts at: 3033 3033
Size: 6029
EcoRV GAT'ATC
Cuts at: 2982 2982
Size: 6029
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4048 4048
Size: 6029
FseI GG_CCGG'CC
Cuts at: 546 546
Size: 6029
FspI TGC'GCA
Cuts at: 3810 3810
Size: 6029
HaeII r_GCGC'y
Cuts at: 392 3463 3711 4941 5712 392
Size: 3071 248 1230 771 709
Fragments arranged by size:
3071 1230 771 709 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2302 2334 2457 2489 2608 2640 3871 3901 3903
Size: 32 123 32 119 32 1231 30 2
Cuts at: 3903 3933 2302
Size: 30 4398
Fragments arranged by size:
4398 1231 123 119 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 801 2049 2199 2465 3068 801
Size: 1248 150 266 603 3762
Fragments arranged by size:
3762 1248 603 266 150
HindIII A'AGCT_T
Cuts at: 1156 1156
Size: 6029
HpaI GTT'AAC
Cuts at: 801 801
Size: 6029
KasI G'GCGC_C
Cuts at: 3459 3707 3459
Size: 248 5781
KpnI G_GTAC'C
Cuts at: 1380 3049 1380
Size: 1669 4360
MluI A'CGCG_T
Cuts at: 5462 5462
Size: 6029
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 843 942 2552 4788 4972 843
Size: 99 1610 2236 184 1900
Fragments arranged by size:
2236 1900 1610 184 99
MscI TGG'CCA
Cuts at: 142 946 1255 1415 2482 3790 142
Size: 804 309 160 1067 1308 2381
Fragments arranged by size:
2381 1308 1067 804 309 160
MslI CAynn'nnrTG
Cuts at: 404 976 1657 1979 2275 2686 4018 4145 404
Size: 572 681 322 296 411 1332 127 2288
Fragments arranged by size:
2288 1332 681 572 411 322 296 127
MspA1I CmG'CkG
Cuts at: 1754 1812 2912 3140 3454 3814 4598 4843 1754
Size: 58 1100 228 314 360 784 245 2940
Fragments arranged by size:
2940 1100 784 360 314 245 228 58
NaeI GCC'GGC
Cuts at: 544 927 4210 544
Size: 383 3283 2363
Fragments arranged by size:
3283 2363 383
NarI GG'CG_CC
Cuts at: 3460 3708 3460
Size: 248 5781
NcoI C'CATG_G
Cuts at: 107 2070 107
Size: 1963 4066
NdeI CA'TA_TG
Cuts at: 1327 1327
Size: 6029
NgoMIV G'CCGG_C
Cuts at: 542 925 4208 542
Size: 383 3283 2363
Fragments arranged by size:
3283 2363 383
NheI G'CTAG_C
Cuts at: 438 438
Size: 6029
NspI r_CATG'y
Cuts at: 925 1677 3082 3582 5185 925
Size: 752 1405 500 1603 1769
Fragments arranged by size:
1769 1603 1405 752 500
PasI CC'CwG_GG
Cuts at: 378 706 2397 378
Size: 328 1691 4010
Fragments arranged by size:
4010 1691 328
PciI A'CATG_T
Cuts at: 5181 5181
Size: 6029
PflMI CCAn_nnn'nTGG
Cuts at: 149 2265 2403 149
Size: 2116 138 3775
Fragments arranged by size:
3775 2116 138
PfoI T'CCnGG_A
Cuts at: 1037 2363 1037
Size: 1326 4703
PmeI GTTT'AAAC
Cuts at: 3028 3028
Size: 6029
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1003 1035 4475 4507 1003
Size: 32 3440 32 2525
Fragments arranged by size:
3440 2525 32 32
PpuMI rG'GwC_Cy
Cuts at: 347 1126 1476 2158 2181 2367 347
Size: 779 350 682 23 186 4009
Fragments arranged by size:
4009 779 682 350 186 23
PspOMI G'GGCC_C
Cuts at: 111 709 1565 111
Size: 598 856 4575
Fragments arranged by size:
4575 856 598
PstI C_TGCA'G
Cuts at: 738 1406 1451 1617 1856 3002 3076 3761 738
Size: 668 45 166 239 1146 74 685 3006
Fragments arranged by size:
3006 1146 685 668 239 166 74 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6029
PvuII CAG'CTG
Cuts at: 1812 3454 3814 1812
Size: 1642 360 4027
Fragments arranged by size:
4027 1642 360
RsrII CG'GwC_CG
Cuts at: 4224 4224
Size: 6029
SacI G_AGCT'C
Cuts at: 1021 1281 1295 2363 2947 3043 1021
Size: 260 14 1068 584 96 4007
Fragments arranged by size:
4007 1068 584 260 96 14
SalI G'TCGA_C
Cuts at: 2197 3066 2197
Size: 869 5160
SanDI GG'GwC_CC
Cuts at: 1126 2158 1126
Size: 1032 4997
SapI GCTCTTCn'nnn_
Cuts at: 4052 4262 4052
Size: 210 5819
SbfI CC_TGCA'GG
Cuts at: 3076 3076
Size: 6029
ScaI AGT'ACT
Cuts at: 2101 5546 2101
Size: 3445 2584
SexAI A'CCwGG_T
Cuts at: 1745 1745
Size: 6029
SfcI C'TryA_G
Cuts at: 33 734 1402 1447 1613 1852 2998 3072 3757
Size: 701 668 45 166 239 1146 74 685
Cuts at: 3757 4725 4916 33
Size: 968 191 1146
Fragments arranged by size:
1146 1146 968 701 685 668 239 191
166 74 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 2785 2785
Size: 6029
SfoI GGC'GCC
Cuts at: 3461 3709 3461
Size: 248 5781
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6029
SmaI CCC'GGG
Cuts at: 3051 3051
Size: 6029
SmlI C'TyrA_G
Cuts at: 1014 2218 3184 4099 4813 5075 1014
Size: 1204 966 915 714 262 1968
Fragments arranged by size:
1968 1204 966 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6029
SphI G_CATG'C
Cuts at: 3082 3582 3082
Size: 500 5529
StuI AGG'CCT
Cuts at: 1087 1097 2289 2779 1087
Size: 10 1192 490 4337
Fragments arranged by size:
4337 1192 490 10
StyI C'CwwG_G
Cuts at: 107 1175 2070 2789 3163 3517 107
Size: 1068 895 719 374 354 2619
Fragments arranged by size:
2619 1068 895 719 374 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 640 2815 3612 3745 4204 4413 640
Size: 2175 797 133 459 209 2256
Fragments arranged by size:
2256 2175 797 459 209 133
TatI w'GTAC_w
Cuts at: 287 1718 2082 2099 5544 287
Size: 1431 364 17 3445 772
Fragments arranged by size:
3445 1431 772 364 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 155 187 203 235 958 990 5546 5578 155
Size: 32 16 32 723 32 4556 32 606
Fragments arranged by size:
4556 723 606 32 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 2596 3826 2596
Size: 1230 4799
XbaI T'CTAG_A
Cuts at: 3060 3060
Size: 6029
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 275 2262 2475 3524 275
Size: 1987 213 1049 2780
Fragments arranged by size:
2780 1987 1049 213
XhoI C'TCGA_G
Cuts at: 2218 2218
Size: 6029
XmaI C'CCGG_G
Cuts at: 3049 3049
Size: 6029
ZraI GAC'GTC
Cuts at: 2619 2619
Size: 6029
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AlfI AloI AscI AvrII
BaeI BfrBI BsiWI BsmI BspEI BssHII BstZ17I ClaI
FspAI MfeI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SgrAI SnaBI SrfI SspI
SwaI XmnI