Restriction map for
pF1KB7023
(Circular) (Six-base) MAPSORT of: pF1KB7023.seq Check: 7126 from: 1 to: 6431
pF1KB7023 6431 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 985 1935 3012 985
Size: 950 1077 4404
Fragments arranged by size:
4404 1077 950
AatII G_ACGT'C
Cuts at: 2390 2390
Size: 6431
AccI GT'mk_AC
Cuts at: 725 3469 725
Size: 2744 3687
Acc65I G'GTAC_C
Cuts at: 3447 3447
Size: 6431
AclI AA'CG_TT
Cuts at: 6271 6271
Size: 6431
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2083 2508 4256 4688 5435 5703 2083
Size: 425 1748 432 747 268 2811
Fragments arranged by size:
2811 1748 747 432 425 268
AfeI AGC'GCT
Cuts at: 1191 1191
Size: 6431
AflIII A'CryG_T
Cuts at: 1220 2762 3783 4887 5864 1220
Size: 1542 1021 1104 977 1787
Fragments arranged by size:
1787 1542 1104 1021 977
AgeI A'CCGG_T
Cuts at: 4819 4819
Size: 6431
AhdI GACnn_n'nnGTC
Cuts at: 2406 2766 2406
Size: 360 6071
AleI CACnn'nnGTG
Cuts at: 635 635
Size: 6431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2281 2716 2281
Size: 435 5996
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1391 1423 1391
Size: 32 6399
AlwNI CAG_nnn'CTG
Cuts at: 204 1279 3736 5303 204
Size: 1075 2457 1567 1332
Fragments arranged by size:
2457 1567 1332 1075
ApaI G_GGCC'C
Cuts at: 2087 3217 2087
Size: 1130 5301
ApaLI G'TGCA_C
Cuts at: 5201 5201
Size: 6431
ApoI r'AATT_y
Cuts at: 3435 5784 6040 3435
Size: 2349 256 3826
Fragments arranged by size:
3826 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6431
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6431
AvaI C'yCGr_G
Cuts at: 219 270 963 2651 2980 3196 3398 3451 219
Size: 51 693 1688 329 216 202 53 3199
Fragments arranged by size:
3199 1688 693 329 216 202 53 51
BamHI G'GATC_C
Cuts at: 2777 3456 2777
Size: 679 5752
BanI G'GyrC_C
Cuts at: 343 1677 2809 3386 3447 3861 4109 4144 343
Size: 1334 1132 577 61 414 248 35 2630
Fragments arranged by size:
2630 1334 1132 577 414 248 61 35
BanII G_rGCy'C
Cuts at: 314 1828 2087 2252 2279 3217 3305 3329 3341
Size: 1514 259 165 27 938 88 24 12
Cuts at: 3341 3445 4475 314
Size: 104 1030 2270
Fragments arranged by size:
2270 1514 1030 938 259 165 104 88
27 24 12
BbeI G_GCGC'C
Cuts at: 3865 4113 3865
Size: 248 6183
BbsI GAAGACnn'nnnn_
Cuts at: 965 965
Size: 6431
BbvCI CC'TCA_GC
Cuts at: 1316 1316
Size: 6431
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 801 835 1591 1625 90
Size: 34 677 34 756 34 4896
Fragments arranged by size:
4896 756 677 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4329 5090 6431 4329
Size: 761 1341 4329
Fragments arranged by size:
4329 1341 761
BclI T'GATC_A
Cuts at: 627 2480 3951 627
Size: 1853 1471 3107
Fragments arranged by size:
3107 1853 1471
BglI GCCn_nnn'nGGC
Cuts at: 1723 2274 1723
Size: 551 5880
BglII A'GATC_T
Cuts at: 191 2630 2792 2903 3946 5983 191
Size: 2439 162 111 1043 2037 639
Fragments arranged by size:
2439 2037 1043 639 162 111
BlpI GC'TnA_GC
Cuts at: 1091 2500 3543 1091
Size: 1409 1043 3979
Fragments arranged by size:
3979 1409 1043
Bme1580I G_kGCm'C
Cuts at: 1078 2087 2366 3217 3391 4056 4149 5205 1078
Size: 1009 279 851 174 665 93 1056 2304
Fragments arranged by size:
2304 1056 1009 851 665 279 174 93
BmgBI CAC'GTC
Cuts at: 2763 2763
Size: 6431
BmrI ACTGGGnnnn_n'
Cuts at: 389 938 1563 3643 3724 4059 6203 389
Size: 549 625 2080 81 335 2144 617
Fragments arranged by size:
2144 2080 625 617 549 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1997 4798 1997
Size: 2801 3630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 336 451 718 1170 1387 1555 2477 3241
Size: 105 115 267 452 217 168 922 764
Cuts at: 3241 3319 3325 231
Size: 78 6 3337
Fragments arranged by size:
3337 922 764 452 267 217 168 115
105 78 6
Bpu10I CC'TnA_GC
Cuts at: 1316 1972 2755 1316
Size: 656 783 4992
Fragments arranged by size:
4992 783 656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3355 3607 4486 4978 5276 3355
Size: 252 879 492 298 4510
Fragments arranged by size:
4510 879 492 298 252
BsaI GGTCTCn'nnnn_
Cuts at: 427 1222 1457 2162 2576 6135 427
Size: 795 235 705 414 3559 723
Fragments arranged by size:
3559 795 723 705 414 235
BsaAI yAC'GTr
Cuts at: 1053 1483 1605 1851 1883 3784 1053
Size: 430 122 246 32 1901 3700
Fragments arranged by size:
3700 1901 430 246 122 32
BsaBI GATnn'nnATC
Cuts at: 6 3945 5982 6
Size: 3939 2037 455
Fragments arranged by size:
3939 2037 455
BsaHI Gr'CG_yC
Cuts at: 2387 3862 4110 4812 6308 2387
Size: 1475 248 702 1496 2510
Fragments arranged by size:
2510 1496 1475 702 248
BsaWI w'CCGG_w
Cuts at: 282 2721 3620 4141 4819 5093 5240 5651 282
Size: 2439 899 521 678 274 147 411 1062
Fragments arranged by size:
2439 1062 899 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 403 433 910 940 1219 1249 1555 1585 2159
Size: 30 477 30 279 30 306 30 574
Cuts at: 2159 2189 3106 3136 403
Size: 30 917 30 3698
Fragments arranged by size:
3698 917 574 477 306 279 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1537 1573 1576 3046 3057 3261 1537
Size: 36 3 1470 11 204 4707
Fragments arranged by size:
4707 1470 204 36 11 3
BseYI C'CCAG_C
Cuts at: 1433 1503 1911 2239 2287 2879 2927 2963 3054
Size: 70 408 328 48 592 48 36 91
Cuts at: 3054 3232 3329 3856 4614 5191 5687 1433
Size: 178 97 527 758 577 496 2177
Fragments arranged by size:
2177 758 592 577 527 496 408 328
178 97 91 70 48 48 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 790 919 1614 2103 2170 3163 571
Size: 219 129 695 489 67 993 3839
Fragments arranged by size:
3839 993 695 489 219 129 67
BsiEI CG_ry'CG
Cuts at: 104 3973 4019 5227 104
Size: 3869 46 1208 1308
Fragments arranged by size:
3869 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 362 1588 1739 1828 2252 2606 3341 3445 4223
Size: 1226 151 89 424 354 735 104 778
Cuts at: 4223 4413 5205 362
Size: 190 792 1588
Fragments arranged by size:
1588 1226 792 778 735 424 354 190
151 104 89
BsmBI CGTCTCn'nnnn_
Cuts at: 759 2378 759
Size: 1619 4812
Bsp1286I G_dGCh'C
Cuts at: 314 362 1078 1588 1739 1828 2087 2252 2279
Size: 48 716 510 151 89 259 165 27
Cuts at: 2279 2366 2606 3217 3305 3329 3341 3391 3445
Size: 87 240 611 88 24 12 50 54
Cuts at: 3445 4056 4149 4223 4413 4475 5205 314
Size: 611 93 74 190 62 730 1540
Fragments arranged by size:
1540 730 716 611 611 510 259 240
190 165 151 93 89 88 87 74
62 54 50 48 27 24 12
BspEI T'CCGG_A
Cuts at: 2721 5651 2721
Size: 2930 3501
BspHI T'CATG_A
Cuts at: 2069 5607 2069
Size: 3538 2893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 274 985 1107 1935 2192 2473 3012 3481 3695
Size: 711 122 828 257 281 539 469 214
Cuts at: 3695 3997 4378 274
Size: 302 381 2327
Fragments arranged by size:
2327 828 711 539 469 381 302 281
257 214 122
BsrBI CCG'CTC
Cuts at: 50 444 926 1046 4723 4777 6261 6426 50
Size: 394 482 120 3677 54 1484 165 55
Fragments arranged by size:
3677 1484 482 394 165 120 55 54
BsrDI GCAATG_nn'
Cuts at: 987 2265 4343 987
Size: 1278 2078 3075
Fragments arranged by size:
3075 2078 1278
BsrFI r'CCGG_y
Cuts at: 885 4429 4610 4819 885
Size: 3544 181 209 2497
Fragments arranged by size:
3544 2497 209 181
BssSI C'ACGA_G
Cuts at: 589 2515 4702 5060 589
Size: 1926 2187 358 1960
Fragments arranged by size:
2187 1960 1926 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3808 3808
Size: 6431
BstBI TT'CG_AA
Cuts at: 4792 4792
Size: 6431
BstEII G'GTnAC_C
Cuts at: 2866 2866
Size: 6431
BstXI CCAn_nnnn'nTGG
Cuts at: 1503 2421 2547 1503
Size: 918 126 5387
Fragments arranged by size:
5387 918 126
BstYI r'GATC_y
Cuts at: 1 191 471 1796 2630 2777 2792 2903 3456
Size: 190 280 1325 834 147 15 111 553
Cuts at: 3456 3923 3946 4281 4527 5539 5625 5637 5983
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5983 1
Size: 449
Fragments arranged by size:
1325 1012 834 553 467 449 346 335
280 246 190 147 111 86 23 15
12
BtgI C'CryG_G
Cuts at: 423 479 1034 1894 1958 3093 423
Size: 56 555 860 64 1135 3761
Fragments arranged by size:
3761 1135 860 555 64 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 139 4381 4562 4725 139
Size: 4242 181 163 1845
Fragments arranged by size:
4242 1845 181 163
BtsI GCAGTG_nn'
Cuts at: 583 610 773 1111 1588 2040 3674 583
Size: 27 163 338 477 452 1634 3340
Fragments arranged by size:
3340 1634 477 452 338 163 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 849 884 1670 1705 849
Size: 35 786 35 5575
Fragments arranged by size:
5575 786 35 35
DraI TTT'AAA
Cuts at: 3430 3430
Size: 6431
DrdI GACnn_nn'nnGTC
Cuts at: 4137 4995 4137
Size: 858 5573
EaeI y'GGCC_r
Cuts at: 326 1281 1419 1612 1833 2000 2537 4016 4190
Size: 955 138 193 221 167 537 1479 174
Cuts at: 4190 4581 4608 5953 326
Size: 391 27 1345 804
Fragments arranged by size:
1479 1345 955 804 537 391 221 193
174 167 138 27
EagI C'GGCC_G
Cuts at: 4016 4016
Size: 6431
EarI CTCTTCn'nnn_
Cuts at: 1514 2927 4454 4664 1514
Size: 1413 1527 210 3281
Fragments arranged by size:
3281 1527 1413 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 772 3418 4639 4949 5095 6241 772
Size: 2646 1221 310 146 1146 962
Fragments arranged by size:
2646 1221 1146 962 310 146
EcoICRI GAG'CTC
Cuts at: 1826 2250 3339 3443 1826
Size: 424 1089 104 4814
Fragments arranged by size:
4814 1089 424 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 336 451 718 1170 1387 1555 2083 2477
Size: 105 115 267 452 217 168 528 394
Cuts at: 2477 2508 3241 3319 3325 4256 4688 5435 5703
Size: 31 733 78 6 931 432 747 268
Cuts at: 5703 231
Size: 959
Fragments arranged by size:
959 931 747 733 528 452 432 394
268 267 217 168 115 105 78 31
6
EcoNI CCTnn'n_nnAGG
Cuts at: 1066 1066
Size: 6431
EcoO109I rG'GnC_Cy
Cuts at: 201 968 1934 2084 2114 2131 2462 3213 3570
Size: 767 966 150 30 17 331 751 357
Cuts at: 3570 201
Size: 3062
Fragments arranged by size:
3062 966 767 751 357 331 150 30
17
EcoRI G'AATT_C
Cuts at: 3435 3435
Size: 6431
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4450 4450
Size: 6431
FspI TGC'GCA
Cuts at: 1639 4212 1639
Size: 2573 3858
HaeII r_GCGC'y
Cuts at: 1193 2232 3865 4113 5135 6114 1193
Size: 1039 1633 248 1022 979 1510
Fragments arranged by size:
1633 1510 1039 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 117 149 1907 1939 2058 2090 2120 2152 2392
Size: 32 1758 32 119 32 30 32 240
Cuts at: 2392 2424 2752 2784 3106 3138 4273 4303 4305
Size: 32 328 32 322 32 1135 30 2
Cuts at: 4305 4335 117
Size: 30 2213
Fragments arranged by size:
2213 1758 1135 328 322 240 119 32
32 32 32 32 32 32 30 30
30 2
HincII GTy'rAC
Cuts at: 837 2103 3264 3470 837
Size: 1266 1161 206 3798
Fragments arranged by size:
3798 1266 1161 206
KasI G'GCGC_C
Cuts at: 3861 4109 3861
Size: 248 6183
KpnI G_GTAC'C
Cuts at: 3451 3451
Size: 6431
MluI A'CGCG_T
Cuts at: 5864 5864
Size: 6431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1205 2036 2246 2438 2921 5102 5286 1205
Size: 831 210 192 483 2181 184 2350
Fragments arranged by size:
2350 2181 831 483 210 192 184
MscI TGG'CCA
Cuts at: 328 1283 2539 4192 328
Size: 955 1256 1653 2567
Fragments arranged by size:
2567 1653 1256 955
MslI CAynn'nnrTG
Cuts at: 463 635 805 1010 1033 1310 1675 1849 2332
Size: 172 170 205 23 277 365 174 483
Cuts at: 2332 4420 4547 463
Size: 2088 127 2347
Fragments arranged by size:
2347 2088 483 365 277 205 174 172
170 127 23
MspA1I CmG'CkG
Cuts at: 573 1412 1533 1866 1896 2967 3542 3856 4216
Size: 839 121 333 30 1071 575 314 360
Cuts at: 4216 5229 5474 573
Size: 1013 245 1530
Fragments arranged by size:
1530 1071 1013 839 575 360 333 314
245 121 30
NaeI GCC'GGC
Cuts at: 4612 4612
Size: 6431
NarI GG'CG_CC
Cuts at: 3862 4110 3862
Size: 248 6183
NcoI C'CATG_G
Cuts at: 3093 3093
Size: 6431
NgoMIV G'CCGG_C
Cuts at: 4610 4610
Size: 6431
NspI r_CATG'y
Cuts at: 1431 3484 3984 4891 1431
Size: 2053 500 907 2971
Fragments arranged by size:
2971 2053 907 500
PasI CC'CwG_GG
Cuts at: 1176 1176
Size: 6431
PciI A'CATG_T
Cuts at: 4887 4887
Size: 6431
PflMI CCAn_nnn'nTGG
Cuts at: 3024 3024
Size: 6431
PmeI GTTT'AAAC
Cuts at: 3430 3430
Size: 6431
PmlI CAC'GTG
Cuts at: 1483 1605 1883 1483
Size: 122 278 6031
Fragments arranged by size:
6031 278 122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5570 5602 5570
Size: 32 6399
PpuMI rG'GwC_Cy
Cuts at: 968 2131 2462 968
Size: 1163 331 4937
Fragments arranged by size:
4937 1163 331
PshAI GACnn'nnGTC
Cuts at: 778 778
Size: 6431
PspOMI G'GGCC_C
Cuts at: 2083 3213 2083
Size: 1130 5301
PstI C_TGCA'G
Cuts at: 1118 1462 1601 2213 3478 4163 1118
Size: 344 139 612 1265 685 3386
Fragments arranged by size:
3386 1265 685 612 344 139
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6431
PvuII CAG'CTG
Cuts at: 573 1533 1866 2967 3856 4216 573
Size: 960 333 1101 889 360 2788
Fragments arranged by size:
2788 1101 960 889 360 333
RsrII CG'GwC_CG
Cuts at: 4626 4626
Size: 6431
SacI G_AGCT'C
Cuts at: 1828 2252 3341 3445 1828
Size: 424 1089 104 4814
Fragments arranged by size:
4814 1089 424 104
SacII CC_GC'GG
Cuts at: 1897 1897
Size: 6431
SalI G'TCGA_C
Cuts at: 3468 3468
Size: 6431
SapI GCTCTTCn'nnn_
Cuts at: 4454 4664 4454
Size: 210 6221
SbfI CC_TGCA'GG
Cuts at: 3478 3478
Size: 6431
ScaI AGT'ACT
Cuts at: 2155 5948 2155
Size: 3793 2638
SexAI A'CCwGG_T
Cuts at: 2171 2531 2171
Size: 360 6071
SfcI C'TryA_G
Cuts at: 33 726 1114 1339 1458 1597 2209 2253 3474
Size: 693 388 225 119 139 612 44 1221
Cuts at: 3474 4159 5152 5343 33
Size: 685 993 191 1121
Fragments arranged by size:
1221 1121 993 693 685 612 388 225
191 139 119 44
SfoI GGC'GCC
Cuts at: 3863 4111 3863
Size: 248 6183
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6431
SmaI CCC'GGG
Cuts at: 221 3453 221
Size: 3232 3199
SmlI C'TyrA_G
Cuts at: 2651 2980 3334 3586 4501 4993 5255 2651
Size: 329 354 252 915 492 262 3827
Fragments arranged by size:
3827 915 492 354 329 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6431
SphI G_CATG'C
Cuts at: 1431 3484 3984 1431
Size: 2053 500 3878
Fragments arranged by size:
3878 2053 500
StuI AGG'CCT
Cuts at: 1132 1132
Size: 6431
StyI C'CwwG_G
Cuts at: 329 2079 2801 3093 3565 3919 329
Size: 1750 722 292 472 354 2841
Fragments arranged by size:
2841 1750 722 472 354 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 424 451 2794 3056 3068 4014 4147 4606 4815
Size: 27 2343 262 12 946 133 459 209
Cuts at: 4815 424
Size: 2040
Fragments arranged by size:
2343 2040 946 459 262 209 133 27
12
TatI w'GTAC_w
Cuts at: 1841 2153 5946 1841
Size: 312 3793 2326
Fragments arranged by size:
3793 2326 312
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 403 435 602 634 910 942 2159 2191 3370
Size: 32 167 32 276 32 1217 32 1179
Cuts at: 3370 3402 5948 5980 403
Size: 32 2546 32 854
Fragments arranged by size:
2546 1217 1179 854 276 167 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4228 4228
Size: 6431
XbaI T'CTAG_A
Cuts at: 3462 3462
Size: 6431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1390 1725 1995 2539 3926 1390
Size: 335 270 544 1387 3895
Fragments arranged by size:
3895 1387 544 335 270
XhoI C'TCGA_G
Cuts at: 2651 2980 2651
Size: 329 6102
XmaI C'CCGG_G
Cuts at: 219 3451 219
Size: 3232 3199
ZraI GAC'GTC
Cuts at: 2388 2388
Size: 6431
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BfrBI BmtI BsiWI BsmI
BsrGI BssHII BstZ17I Bsu36I ClaI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PsiI PspXI PsrI SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XmnI