Restriction map for
pF1KB7031
(Circular) (Six-base) MAPSORT of: pF1KB7031.seq Check: 801 from: 1 to: 4232
pF1KB7031 4232 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 245 1270 245
Size: 1025 3207
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 669 972 2057 2489 3236 3504 669
Size: 303 1085 432 747 268 1397
Fragments arranged by size:
1397 1085 747 432 303 268
AflIII A'CryG_T
Cuts at: 473 1584 2688 3665 473
Size: 1111 1104 977 1040
Fragments arranged by size:
1111 1104 1040 977
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AleI CACnn'nnGTG
Cuts at: 835 835
Size: 4232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 544 544
Size: 4232
AlwNI CAG_nnn'CTG
Cuts at: 1537 3104 1537
Size: 1567 2665
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 1236 3585 3841 1236
Size: 2349 256 1627
Fragments arranged by size:
2349 1627 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 265 1252 265
Size: 987 3245
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 926 1248 1662 1910 1945 926
Size: 322 414 248 35 3213
Fragments arranged by size:
3213 414 322 248 35
BanII G_rGCy'C
Cuts at: 959 1246 2276 959
Size: 287 1030 2915
Fragments arranged by size:
2915 1030 287
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BbsI GAAGACnn'nnnn_
Cuts at: 325 369 480 627 698 978 325
Size: 44 111 147 71 280 3579
Fragments arranged by size:
3579 280 147 111 71 44
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 290 1752 290
Size: 1462 2770
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 1344 1344
Size: 4232
Bme1580I G_kGCm'C
Cuts at: 931 1857 1950 3006 931
Size: 926 93 1056 2157
Fragments arranged by size:
2157 1056 926 93
BmrI ACTGGGnnnn_n'
Cuts at: 429 796 1444 1525 1860 4004 429
Size: 367 648 81 335 2144 657
Fragments arranged by size:
2144 657 648 367 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 4232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 636 1408 2287 2779 3077 636
Size: 772 879 492 298 1791
Fragments arranged by size:
1791 879 772 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1207 3936 1207
Size: 2729 1503
BsaAI yAC'GTr
Cuts at: 1585 1585
Size: 4232
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 1663 1911 2613 4109 1663
Size: 248 702 1496 1786
Fragments arranged by size:
1786 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1421 1942 2620 2894 3041 3452 1421
Size: 521 678 274 147 411 2201
Fragments arranged by size:
2201 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 932 962 932
Size: 30 4202
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 523 523
Size: 4232
BseYI C'CCAG_C
Cuts at: 1150 1657 2415 2992 3488 1150
Size: 507 758 577 496 1894
Fragments arranged by size:
1894 758 577 507 496
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3028 104
Size: 1670 46 1208 1308
Fragments arranged by size:
1670 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 533 959 1246 2024 2214 3006 533
Size: 426 287 778 190 792 1759
Fragments arranged by size:
1759 792 778 426 287 190
Bsp1286I G_dGCh'C
Cuts at: 533 931 959 1246 1857 1950 2024 2214 2276
Size: 398 28 287 611 93 74 190 62
Cuts at: 2276 3006 533
Size: 730 1759
Fragments arranged by size:
1759 730 611 398 287 190 93 74
62 28
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 662 1282 1496 1798 2179 662
Size: 620 214 302 381 2715
Fragments arranged by size:
2715 620 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2524 2578 4062 4227 50
Size: 2474 54 1484 165 55
Fragments arranged by size:
2474 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 285 296 1022 2144 285
Size: 11 726 1122 2373
Fragments arranged by size:
2373 1122 726 11
BsrFI r'CCGG_y
Cuts at: 752 1216 2230 2411 2620 752
Size: 464 1014 181 209 2364
Fragments arranged by size:
2364 1014 464 209 181
BssSI C'ACGA_G
Cuts at: 711 2503 2861 711
Size: 1792 358 2082
Fragments arranged by size:
2082 1792 358
BstAPI GCAn_nnn'nTGC
Cuts at: 257 1609 257
Size: 1352 2880
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstEII G'GTnAC_C
Cuts at: 219 219
Size: 4232
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4232
BstYI r'GATC_y
Cuts at: 1 227 1257 1724 1747 2082 2328 3340 3426
Size: 226 1030 467 23 335 246 1012 86
Cuts at: 3426 3438 3784 1
Size: 12 346 449
Fragments arranged by size:
1030 1012 467 449 346 335 246 226
86 23 12
BtgI C'CryG_G
Cuts at: 407 407
Size: 4232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2182 2363 2526 2182
Size: 181 163 3888
Fragments arranged by size:
3888 181 163
BtsI GCAGTG_nn'
Cuts at: 359 965 1010 1475 359
Size: 606 45 465 3116
Fragments arranged by size:
3116 606 465 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 999 1034 999
Size: 35 4197
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DraIII CAC_nnn'GTG
Cuts at: 762 762
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 1938 2796 1938
Size: 858 3374
EaeI y'GGCC_r
Cuts at: 863 1007 1817 1991 2382 2409 3754 863
Size: 144 810 174 391 27 1345 1341
Fragments arranged by size:
1345 1341 810 391 174 144 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 1102 1146 2255 2465 1102
Size: 44 1109 210 2869
Fragments arranged by size:
2869 1109 210 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2440 2750 2896 4042 2440
Size: 310 146 1146 2630
Fragments arranged by size:
2630 1146 310 146
EcoICRI GAG'CTC
Cuts at: 957 1244 957
Size: 287 3945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 669 972 2057 2489 3236 3504 669
Size: 303 1085 432 747 268 1397
Fragments arranged by size:
1397 1085 747 432 303 268
EcoO109I rG'GnC_Cy
Cuts at: 600 1371 600
Size: 771 3461
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 897 2251 897
Size: 1354 2878
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 1666 1914 2936 3915 1666
Size: 248 1022 979 1983
Fragments arranged by size:
1983 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 2074 2104 2106 2136 184
Size: 32 1858 30 2 30 2280
Fragments arranged by size:
2280 1858 32 30 30 2
HincII GTy'rAC
Cuts at: 1271 1271
Size: 4232
HindIII A'AGCT_T
Cuts at: 1161 1161
Size: 4232
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3087 2903
Size: 184 4048
MscI TGG'CCA
Cuts at: 865 1009 1993 865
Size: 144 984 3104
Fragments arranged by size:
3104 984 144
MslI CAynn'nnrTG
Cuts at: 112 526 763 835 970 2221 2348 112
Size: 414 237 72 135 1251 127 1996
Fragments arranged by size:
1996 1251 414 237 135 127 72
MspA1I CmG'CkG
Cuts at: 518 1034 1343 1657 2017 3030 3275 518
Size: 516 309 314 360 1013 245 1475
Fragments arranged by size:
1475 1013 516 360 314 309 245
NaeI GCC'GGC
Cuts at: 754 2413 754
Size: 1659 2573
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NcoI C'CATG_G
Cuts at: 407 407
Size: 4232
NgoMIV G'CCGG_C
Cuts at: 752 2411 752
Size: 1659 2573
NspI r_CATG'y
Cuts at: 477 1224 1285 1785 2692 477
Size: 747 61 500 907 2017
Fragments arranged by size:
2017 907 747 500 61
PciI A'CATG_T
Cuts at: 473 2688 473
Size: 2215 2017
PfoI T'CCnGG_A
Cuts at: 638 638
Size: 4232
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PpuMI rG'GwC_Cy
Cuts at: 600 600
Size: 4232
PstI C_TGCA'G
Cuts at: 851 1279 1964 851
Size: 428 685 3119
Fragments arranged by size:
3119 685 428
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 1034 1657 2017 1034
Size: 623 360 3249
Fragments arranged by size:
3249 623 360
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 959 1246 959
Size: 287 3945
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SfcI C'TryA_G
Cuts at: 33 246 847 1275 1960 2953 3144 33
Size: 213 601 428 685 993 191 1121
Fragments arranged by size:
1121 993 685 601 428 213 191
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 1254 1254
Size: 4232
SmlI C'TyrA_G
Cuts at: 615 1387 2302 2794 3056 615
Size: 772 915 492 262 1791
Fragments arranged by size:
1791 915 772 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 1224 1285 1785 1224
Size: 61 500 3671
Fragments arranged by size:
3671 500 61
StyI C'CwwG_G
Cuts at: 206 407 1366 1720 206
Size: 201 959 354 2718
Fragments arranged by size:
2718 959 354 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1815 1948 2407 2616 1815
Size: 133 459 209 3431
Fragments arranged by size:
3431 459 209 133
TatI w'GTAC_w
Cuts at: 3747 3747
Size: 4232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 932 964 3749 3781 932
Size: 32 2785 32 1383
Fragments arranged by size:
2785 1383 32 32
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 186 231 1727 186
Size: 45 1496 2691
Fragments arranged by size:
2691 1496 45
XmaI C'CCGG_G
Cuts at: 1252 1252
Size: 4232
XmnI GAAnn'nnTTC
Cuts at: 880 880
Size: 4232
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI