Restriction map for
pF1KB7033
(Circular) (Six-base) MAPSORT of: pF1KB7033.seq Check: 8686 from: 1 to: 4385
pF1KB7033 4385 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 357 893 357
Size: 536 3849
AccI GT'mk_AC
Cuts at: 1423 1423
Size: 4385
Acc65I G'GTAC_C
Cuts at: 1401 1401
Size: 4385
AclI AA'CG_TT
Cuts at: 1379 4225 1379
Size: 2846 1539
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2642 3389 3657 2210
Size: 432 747 268 2938
Fragments arranged by size:
2938 747 432 268
AfeI AGC'GCT
Cuts at: 1012 1078 1012
Size: 66 4319
AflIII A'CryG_T
Cuts at: 402 1016 1737 2841 3818 402
Size: 614 721 1104 977 969
Fragments arranged by size:
1104 977 969 721 614
AgeI A'CCGG_T
Cuts at: 2773 2773
Size: 4385
AleI CACnn'nnGTG
Cuts at: 178 251 994 1097 178
Size: 73 743 103 3466
Fragments arranged by size:
3466 743 103 73
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1012 1012
Size: 4385
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 475 507 475
Size: 32 4353
AlwNI CAG_nnn'CTG
Cuts at: 633 1200 1233 1288 1309 1690 3257 633
Size: 567 33 55 21 381 1567 1761
Fragments arranged by size:
1761 1567 567 381 55 33 21
ApaI G_GGCC'C
Cuts at: 1249 1249
Size: 4385
ApaLI G'TGCA_C
Cuts at: 406 645 3155 406
Size: 239 2510 1636
Fragments arranged by size:
2510 1636 239
ApoI r'AATT_y
Cuts at: 742 1389 3738 3994 742
Size: 647 2349 256 1133
Fragments arranged by size:
2349 1133 647 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4385
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 916 1405 916
Size: 489 3896
BamHI G'GATC_C
Cuts at: 1410 1410
Size: 4385
BanI G'GyrC_C
Cuts at: 245 890 1274 1401 1815 2063 2098 245
Size: 645 384 127 414 248 35 2532
Fragments arranged by size:
2532 645 414 384 248 127 35
BanII G_rGCy'C
Cuts at: 710 1249 1399 2429 710
Size: 539 150 1030 2666
Fragments arranged by size:
2666 1030 539 150
BbeI G_GCGC'C
Cuts at: 1278 1819 2067 1278
Size: 541 248 3596
Fragments arranged by size:
3596 541 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4351
BciVI GTATCCnnnnn_n'
Cuts at: 2283 3044 4385 2283
Size: 761 1341 2283
Fragments arranged by size:
2283 1341 761
BclI T'GATC_A
Cuts at: 1905 1905
Size: 4385
BglI GCCn_nnn'nGGC
Cuts at: 229 1219 1267 1283 229
Size: 990 48 16 3331
Fragments arranged by size:
3331 990 48 16
BglII A'GATC_T
Cuts at: 1900 3937 1900
Size: 2037 2348
BlpI GC'TnA_GC
Cuts at: 128 259 340 1238 1497 128
Size: 131 81 898 259 3016
Fragments arranged by size:
3016 898 259 131 81
Bme1580I G_kGCm'C
Cuts at: 410 649 1249 2010 2103 3159 410
Size: 239 600 761 93 1056 1636
Fragments arranged by size:
1636 1056 761 600 239 93
BmrI ACTGGGnnnn_n'
Cuts at: 1597 1678 2013 4157 1597
Size: 81 335 2144 1825
Fragments arranged by size:
2144 1825 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 4385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 684 898 1105 1265 406
Size: 278 214 207 160 3526
Fragments arranged by size:
3526 278 214 207 160
Bpu10I CC'TnA_GC
Cuts at: 152 152
Size: 4385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 694 928 1561 2440 2932 3230 595
Size: 99 234 633 879 492 298 1750
Fragments arranged by size:
1750 879 633 492 298 234 99
BsaI GGTCTCn'nnnn_
Cuts at: 4089 4089
Size: 4385
BsaAI yAC'GTr
Cuts at: 444 624 1323 1738 444
Size: 180 699 415 3091
Fragments arranged by size:
3091 699 415 180
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 732 1275 1816 2064 2766 4262 732
Size: 543 541 248 702 1496 855
Fragments arranged by size:
1496 855 702 543 541 248
BsaWI w'CCGG_w
Cuts at: 1574 2095 2773 3047 3194 3605 1574
Size: 521 678 274 147 411 2354
Fragments arranged by size:
2354 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 697 727 1006 1036 697
Size: 30 279 30 4046
Fragments arranged by size:
4046 279 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 403 931 934 403
Size: 528 3 3854
Fragments arranged by size:
3854 528 3
BseYI C'CCAG_C
Cuts at: 985 1248 1810 2568 3145 3641 985
Size: 263 562 758 577 496 1729
Fragments arranged by size:
1729 758 577 562 496 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 385 433 646 1126 367
Size: 18 48 213 480 3626
Fragments arranged by size:
3626 480 213 48 18
BsiEI CG_ry'CG
Cuts at: 104 575 1927 1973 3181 104
Size: 471 1352 46 1208 1308
Fragments arranged by size:
1352 1308 1208 471 46
BsiHKAI G_wGCw'C
Cuts at: 410 649 710 802 884 1024 1165 1399 2177
Size: 239 61 92 82 140 141 234 778
Cuts at: 2177 2367 3159 410
Size: 190 792 1636
Fragments arranged by size:
1636 792 778 239 234 190 141 140
92 82 61
BsmBI CGTCTCn'nnnn_
Cuts at: 723 723
Size: 4385
Bsp1286I G_dGCh'C
Cuts at: 410 649 710 802 884 1024 1165 1249 1399
Size: 239 61 92 82 140 141 84 150
Cuts at: 1399 2010 2103 2177 2367 2429 3159 410
Size: 611 93 74 190 62 730 1636
Fragments arranged by size:
1636 730 611 239 190 150 141 140
93 92 84 82 74 62 61
BspEI T'CCGG_A
Cuts at: 3605 3605
Size: 4385
BspHI T'CATG_A
Cuts at: 1175 3561 1175
Size: 2386 1999
BspMI ACCTGCnnnn'nnnn_
Cuts at: 357 512 893 1435 1649 1951 2332 357
Size: 155 381 542 214 302 381 2410
Fragments arranged by size:
2410 542 381 381 302 214 155
BsrBI CCG'CTC
Cuts at: 50 122 692 2677 2731 4215 4380 50
Size: 72 570 1985 54 1484 165 55
Fragments arranged by size:
1985 1484 570 165 72 55 54
BsrDI GCAATG_nn'
Cuts at: 1208 2297 1208
Size: 1089 3296
BsrFI r'CCGG_y
Cuts at: 570 574 2383 2564 2773 570
Size: 4 1809 181 209 2182
Fragments arranged by size:
2182 1809 209 181 4
BssHII G'CGCG_C
Cuts at: 999 999
Size: 4385
BssSI C'ACGA_G
Cuts at: 1183 2656 3014 1183
Size: 1473 358 2554
Fragments arranged by size:
2554 1473 358
BstAPI GCAn_nnn'nTGC
Cuts at: 363 414 1169 1762 363
Size: 51 755 593 2986
Fragments arranged by size:
2986 755 593 51
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstXI CCAn_nnnn'nTGG
Cuts at: 180 540 1099 180
Size: 360 559 3466
Fragments arranged by size:
3466 559 360
BstYI r'GATC_y
Cuts at: 1 477 923 1410 1877 1900 2235 2481 3493
Size: 476 446 487 467 23 335 246 1012
Cuts at: 3493 3579 3591 3937 1
Size: 86 12 346 449
Fragments arranged by size:
1012 487 476 467 449 446 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 173 185 173
Size: 12 4373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 885 2335 2516 2679 885
Size: 1450 181 163 2591
Fragments arranged by size:
2591 1450 181 163
BtsI GCAGTG_nn'
Cuts at: 516 545 1138 1256 1628 516
Size: 29 593 118 372 3273
Fragments arranged by size:
3273 593 372 118 29
DraI TTT'AAA
Cuts at: 1384 1384
Size: 4385
DraIII CAC_nnn'GTG
Cuts at: 414 1149 1169 414
Size: 735 20 3630
Fragments arranged by size:
3630 735 20
DrdI GACnn_nn'nnGTC
Cuts at: 2091 2949 2091
Size: 858 3527
EaeI y'GGCC_r
Cuts at: 170 182 265 572 792 1970 2144 2535 2562
Size: 12 83 307 220 1178 174 391 27
Cuts at: 2562 3907 170
Size: 1345 648
Fragments arranged by size:
1345 1178 648 391 307 220 174 83
27 12
EagI C'GGCC_G
Cuts at: 572 1970 572
Size: 1398 2987
EarI CTCTTCn'nnn_
Cuts at: 296 2408 2618 296
Size: 2112 210 2063
Fragments arranged by size:
2112 2063 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2593 2903 3049 4195 2593
Size: 310 146 1146 2783
Fragments arranged by size:
2783 1146 310 146
EcoICRI GAG'CTC
Cuts at: 708 1397 708
Size: 689 3696
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 684 898 1105 1265 2210 2642 3389 3657
Size: 278 214 207 160 945 432 747 268
Cuts at: 3657 406
Size: 1134
Fragments arranged by size:
1134 945 747 432 278 268 214 207
160
EcoO109I rG'GnC_Cy
Cuts at: 873 1245 1524 873
Size: 372 279 3734
Fragments arranged by size:
3734 372 279
EcoRI G'AATT_C
Cuts at: 742 1389 742
Size: 647 3738
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4385
FspI TGC'GCA
Cuts at: 508 2166 508
Size: 1658 2727
HaeII r_GCGC'y
Cuts at: 1014 1080 1191 1225 1278 1819 2067 3089 4068
Size: 66 111 34 53 541 248 1022 979
Cuts at: 4068 1014
Size: 1331
Fragments arranged by size:
1331 1022 979 541 248 111 66 53
34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 211 243 2227 2257 2259 2289 211
Size: 32 1984 30 2 30 2307
Fragments arranged by size:
2307 1984 32 30 30 2
HincII GTy'rAC
Cuts at: 1155 1424 1155
Size: 269 4116
KasI G'GCGC_C
Cuts at: 1274 1815 2063 1274
Size: 541 248 3596
Fragments arranged by size:
3596 541 248
KpnI G_GTAC'C
Cuts at: 1405 1405
Size: 4385
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 95 707 3056 3240 95
Size: 612 2349 184 1240
Fragments arranged by size:
2349 1240 612 184
MscI TGG'CCA
Cuts at: 184 267 794 2146 184
Size: 83 527 1352 2423
Fragments arranged by size:
2423 1352 527 83
MslI CAynn'nnrTG
Cuts at: 178 251 811 994 1097 2374 2501 178
Size: 73 560 183 103 1277 127 2062
Fragments arranged by size:
2062 1277 560 183 127 103 73
MspA1I CmG'CkG
Cuts at: 433 528 662 1062 1252 1496 1810 2170 3183
Size: 95 134 400 190 244 314 360 1013
Cuts at: 3183 3428 433
Size: 245 1390
Fragments arranged by size:
1390 1013 400 360 314 245 244 190
134 95
NaeI GCC'GGC
Cuts at: 572 2566 572
Size: 1994 2391
NarI GG'CG_CC
Cuts at: 1275 1816 2064 1275
Size: 541 248 3596
Fragments arranged by size:
3596 541 248
NgoMIV G'CCGG_C
Cuts at: 570 2564 570
Size: 1994 2391
NspI r_CATG'y
Cuts at: 406 1020 1438 1938 2845 406
Size: 614 418 500 907 1946
Fragments arranged by size:
1946 907 614 500 418
PciI A'CATG_T
Cuts at: 402 1016 2841 402
Size: 614 1825 1946
Fragments arranged by size:
1946 1825 614
PflMI CCAn_nnn'nTGG
Cuts at: 960 1200 960
Size: 240 4145
PfoI T'CCnGG_A
Cuts at: 770 770
Size: 4385
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PmlI CAC'GTG
Cuts at: 444 444
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 4353
PspOMI G'GGCC_C
Cuts at: 1245 1245
Size: 4385
PstI C_TGCA'G
Cuts at: 1010 1229 1432 2117 1010
Size: 219 203 685 3278
Fragments arranged by size:
3278 685 219 203
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 433 528 1252 1810 2170 433
Size: 95 724 558 360 2648
Fragments arranged by size:
2648 724 558 360 95
RsrII CG'GwC_CG
Cuts at: 657 2580 657
Size: 1923 2462
SacI G_AGCT'C
Cuts at: 710 1399 710
Size: 689 3696
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 296 2408 2618 296
Size: 2112 210 2063
Fragments arranged by size:
2112 2063 210
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 949 3902 949
Size: 2953 1432
SexAI A'CCwGG_T
Cuts at: 485 1049 485
Size: 564 3821
SfcI C'TryA_G
Cuts at: 33 1006 1225 1428 2113 3106 3297 33
Size: 973 219 203 685 993 191 1121
Fragments arranged by size:
1121 993 973 685 219 203 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 229 229
Size: 4385
SfoI GGC'GCC
Cuts at: 1276 1817 2065 1276
Size: 541 248 3596
Fragments arranged by size:
3596 541 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 1407 1407
Size: 4385
SmlI C'TyrA_G
Cuts at: 610 709 943 1540 2455 2947 3209 610
Size: 99 234 597 915 492 262 1786
Fragments arranged by size:
1786 915 597 492 262 234 99
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 1438 1938 1438
Size: 500 3885
StuI AGG'CCT
Cuts at: 223 823 223
Size: 600 3785
StyI C'CwwG_G
Cuts at: 533 1519 1873 533
Size: 986 354 3045
Fragments arranged by size:
3045 986 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 845 1968 2101 2560 2769 845
Size: 1123 133 459 209 2461
Fragments arranged by size:
2461 1123 459 209 133
TatI w'GTAC_w
Cuts at: 947 3900 947
Size: 2953 1432
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 389 421 897 929 3902 3934 389
Size: 32 476 32 2973 32 840
Fragments arranged by size:
2973 840 476 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 1416 1416
Size: 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1263 1880 1263
Size: 617 3768
XmaI C'CCGG_G
Cuts at: 1405 1405
Size: 4385
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BbsI BbvCI
BfrBI BmgBI BmtI BsiWI BsmI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI