Restriction map for
pF1KB7034
(Circular) (Six-base) MAPSORT of: pF1KB7034.seq Check: 8041 from: 1 to: 4388
pF1KB7034 4388 bp
With 182 enzymes: SgfI *
November 11, 2016 16:52 ..
AccI GT'mk_AC
Cuts at: 1253 1426 1253
Size: 173 4215
Acc65I G'GTAC_C
Cuts at: 1404 1404
Size: 4388
AclI AA'CG_TT
Cuts at: 4228 4228
Size: 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 2213 2645 3392 3660 129
Size: 2084 432 747 268 857
Fragments arranged by size:
2084 857 747 432 268
AflIII A'CryG_T
Cuts at: 662 1740 2844 3821 662
Size: 1078 1104 977 1229
Fragments arranged by size:
1229 1104 1078 977
AgeI A'CCGG_T
Cuts at: 704 2776 704
Size: 2072 2316
AleI CACnn'nnGTG
Cuts at: 439 439
Size: 4388
AlwNI CAG_nnn'CTG
Cuts at: 404 1057 1150 1693 3260 404
Size: 653 93 543 1567 1532
Fragments arranged by size:
1567 1532 653 543 93
ApaI G_GGCC'C
Cuts at: 186 186
Size: 4388
ApaLI G'TGCA_C
Cuts at: 3158 3158
Size: 4388
ApoI r'AATT_y
Cuts at: 1392 3741 3997 1392
Size: 2349 256 1783
Fragments arranged by size:
2349 1783 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 179 847 1034 1408 179
Size: 668 187 374 3159
Fragments arranged by size:
3159 668 374 187
BamHI G'GATC_C
Cuts at: 1413 1413
Size: 4388
BanI G'GyrC_C
Cuts at: 843 1404 1818 2066 2101 843
Size: 561 414 248 35 3130
Fragments arranged by size:
3130 561 414 248 35
BanII G_rGCy'C
Cuts at: 186 854 1402 2432 186
Size: 668 548 1030 2142
Fragments arranged by size:
2142 1030 668 548
BbeI G_GCGC'C
Cuts at: 1822 2070 1822
Size: 248 4140
BbsI GAAGACnn'nnnn_
Cuts at: 930 930
Size: 4388
BciVI GTATCCnnnnn_n'
Cuts at: 734 1326 2286 3047 4388 734
Size: 592 960 761 1341 734
Fragments arranged by size:
1341 960 761 734 592
BclI T'GATC_A
Cuts at: 956 1908 956
Size: 952 3436
BglI GCCn_nnn'nGGC
Cuts at: 120 787 120
Size: 667 3721
BglII A'GATC_T
Cuts at: 1903 3940 1903
Size: 2037 2351
BlpI GC'TnA_GC
Cuts at: 1500 1500
Size: 4388
Bme1580I G_kGCm'C
Cuts at: 186 848 2013 2106 3162 186
Size: 662 1165 93 1056 1412
Fragments arranged by size:
1412 1165 1056 662 93
BmrI ACTGGGnnnn_n'
Cuts at: 1600 1681 2016 4160 1600
Size: 81 335 2144 1828
Fragments arranged by size:
2144 1828 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1564 2443 2935 3233 808
Size: 756 879 492 298 1963
Fragments arranged by size:
1963 879 756 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4092 4092
Size: 4388
BsaAI yAC'GTr
Cuts at: 1741 1741
Size: 4388
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 1819 2067 2769 4265 1819
Size: 248 702 1496 1942
Fragments arranged by size:
1942 1496 702 248
BsaWI w'CCGG_w
Cuts at: 385 436 704 1577 2098 2776 3050 3197 3608
Size: 51 268 873 521 678 274 147 411
Cuts at: 3608 385
Size: 1165
Fragments arranged by size:
1165 873 678 521 411 274 268 147
51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 431 461 431
Size: 30 4358
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 298 811 1159 298
Size: 513 348 3527
Fragments arranged by size:
3527 513 348
BseYI C'CCAG_C
Cuts at: 1288 1813 2571 3148 3644 1288
Size: 525 758 577 496 2032
Fragments arranged by size:
2032 758 577 525 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 517
Size: 4388
BsiEI CG_ry'CG
Cuts at: 104 1930 1976 3184 104
Size: 1826 46 1208 1308
Fragments arranged by size:
1826 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 572 881 1402 2180 2370 3162 572
Size: 309 521 778 190 792 1798
Fragments arranged by size:
1798 792 778 521 309 190
BsmI GAATG_Cn'
Cuts at: 364 364
Size: 4388
Bsp1286I G_dGCh'C
Cuts at: 186 572 848 854 881 1402 2013 2106 2180
Size: 386 276 6 27 521 611 93 74
Cuts at: 2180 2370 2432 3162 186
Size: 190 62 730 1412
Fragments arranged by size:
1412 730 611 521 386 276 190 93
74 62 27 6
BspEI T'CCGG_A
Cuts at: 385 3608 385
Size: 3223 1165
BspHI T'CATG_A
Cuts at: 683 870 3564 683
Size: 187 2694 1507
Fragments arranged by size:
2694 1507 187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1345 1438 1652 1954 2335 1345
Size: 93 214 302 381 3398
Fragments arranged by size:
3398 381 302 214 93
BsrBI CCG'CTC
Cuts at: 50 2680 2734 4218 4383 50
Size: 2630 54 1484 165 55
Fragments arranged by size:
2630 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2300 2300
Size: 4388
BsrFI r'CCGG_y
Cuts at: 513 704 2386 2567 2776 513
Size: 191 1682 181 209 2125
Fragments arranged by size:
2125 1682 209 191 181
BsrGI T'GTAC_A
Cuts at: 431 524 431
Size: 93 4295
BssSI C'ACGA_G
Cuts at: 2659 3017 2659
Size: 358 4030
BstAPI GCAn_nnn'nTGC
Cuts at: 211 1196 1765 211
Size: 985 569 2834
Fragments arranged by size:
2834 985 569
BstBI TT'CG_AA
Cuts at: 2749 2749
Size: 4388
BstYI r'GATC_y
Cuts at: 1 222 1413 1880 1903 2238 2484 3496 3582
Size: 221 1191 467 23 335 246 1012 86
Cuts at: 3582 3594 3940 1
Size: 12 346 449
Fragments arranged by size:
1191 1012 467 449 346 335 246 221
86 23 12
BtgI C'CryG_G
Cuts at: 107 115 542 965 107
Size: 8 427 423 3530
Fragments arranged by size:
3530 427 423 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 783 2338 2519 2682 783
Size: 1555 181 163 2489
Fragments arranged by size:
2489 1555 181 163
BtsI GCAGTG_nn'
Cuts at: 609 641 1311 1631 609
Size: 32 670 320 3366
Fragments arranged by size:
3366 670 320 32
DraI TTT'AAA
Cuts at: 1387 1387
Size: 4388
DrdI GACnn_nn'nnGTC
Cuts at: 2094 2952 2094
Size: 858 3530
EaeI y'GGCC_r
Cuts at: 134 473 539 779 794 1973 2147 2538 2565
Size: 339 66 240 15 1179 174 391 27
Cuts at: 2565 3910 134
Size: 1345 612
Fragments arranged by size:
1345 1179 612 391 339 240 174 66
27 15
EagI C'GGCC_G
Cuts at: 1973 1973
Size: 4388
EarI CTCTTCn'nnn_
Cuts at: 350 1008 2411 2621 350
Size: 658 1403 210 2117
Fragments arranged by size:
2117 1403 658 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2596 2906 3052 4198 2596
Size: 310 146 1146 2786
Fragments arranged by size:
2786 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1400 1400
Size: 4388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 2213 2645 3392 3660 129
Size: 2084 432 747 268 857
Fragments arranged by size:
2084 857 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1527 1527
Size: 4388
EcoRI G'AATT_C
Cuts at: 1392 1392
Size: 4388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4388
FspI TGC'GCA
Cuts at: 2169 2169
Size: 4388
HaeII r_GCGC'y
Cuts at: 1822 2070 3092 4071 1822
Size: 248 1022 979 2139
Fragments arranged by size:
2139 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 214 246 1324 1356 2230 2260 2262 2292 214
Size: 32 1078 32 874 30 2 30 2310
Fragments arranged by size:
2310 1078 874 32 32 30 30 2
HincII GTy'rAC
Cuts at: 305 1379 1427 305
Size: 1074 48 3266
Fragments arranged by size:
3266 1074 48
HpaI GTT'AAC
Cuts at: 305 1379 305
Size: 1074 3314
KasI G'GCGC_C
Cuts at: 1818 2066 1818
Size: 248 4140
KpnI G_GTAC'C
Cuts at: 1408 1408
Size: 4388
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3059 3243 3059
Size: 184 4204
MscI TGG'CCA
Cuts at: 475 541 781 796 2149 475
Size: 66 240 15 1353 2714
Fragments arranged by size:
2714 1353 240 66 15
MslI CAynn'nnrTG
Cuts at: 439 481 841 2377 2504 439
Size: 42 360 1536 127 2323
Fragments arranged by size:
2323 1536 360 127 42
MspA1I CmG'CkG
Cuts at: 117 162 954 1054 1499 1813 2173 3186 3431
Size: 45 792 100 445 314 360 1013 245
Cuts at: 3431 117
Size: 1074
Fragments arranged by size:
1074 1013 792 445 360 314 245 100
45
NaeI GCC'GGC
Cuts at: 515 2569 515
Size: 2054 2334
NarI GG'CG_CC
Cuts at: 1819 2067 1819
Size: 248 4140
NcoI C'CATG_G
Cuts at: 107 107
Size: 4388
NgoMIV G'CCGG_C
Cuts at: 513 2567 513
Size: 2054 2334
NspI r_CATG'y
Cuts at: 531 666 1441 1941 2848 531
Size: 135 775 500 907 2071
Fragments arranged by size:
2071 907 775 500 135
PciI A'CATG_T
Cuts at: 662 2844 662
Size: 2182 2206
PflMI CCAn_nnn'nTGG
Cuts at: 404 404
Size: 4388
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 4356
PsiI TTA'TAA
Cuts at: 1298 1298
Size: 4388
PspOMI G'GGCC_C
Cuts at: 182 182
Size: 4388
PstI C_TGCA'G
Cuts at: 648 1435 2120 648
Size: 787 685 2916
Fragments arranged by size:
2916 787 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4388
PvuII CAG'CTG
Cuts at: 1054 1813 2173 1054
Size: 759 360 3269
Fragments arranged by size:
3269 759 360
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 1402 1402
Size: 4388
SacII CC_GC'GG
Cuts at: 118 118
Size: 4388
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SapI GCTCTTCn'nnn_
Cuts at: 1008 2411 2621 1008
Size: 1403 210 2775
Fragments arranged by size:
2775 1403 210
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 3905 3905
Size: 4388
SfcI C'TryA_G
Cuts at: 33 644 1254 1431 2116 3109 3300 33
Size: 611 610 177 685 993 191 1121
Fragments arranged by size:
1121 993 685 611 610 191 177
SfoI GGC'GCC
Cuts at: 1820 2068 1820
Size: 248 4140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 181 849 1036 1410 181
Size: 668 187 374 3159
Fragments arranged by size:
3159 668 374 187
SmlI C'TyrA_G
Cuts at: 823 1543 2458 2950 3212 823
Size: 720 915 492 262 1999
Fragments arranged by size:
1999 915 720 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 1441 1941 1441
Size: 500 3888
SrfI GCCC'GGGC
Cuts at: 849 849
Size: 4388
StyI C'CwwG_G
Cuts at: 107 1522 1876 107
Size: 1415 354 2619
Fragments arranged by size:
2619 1415 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1971 2104 2563 2772 1971
Size: 133 459 209 3587
Fragments arranged by size:
3587 459 209 133
TatI w'GTAC_w
Cuts at: 431 524 3903 431
Size: 93 3379 916
Fragments arranged by size:
3379 916 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 207 239 404 436 3905 3937 207
Size: 32 165 32 3469 32 658
Fragments arranged by size:
3469 658 165 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2185 2185
Size: 4388
XbaI T'CTAG_A
Cuts at: 1419 1419
Size: 4388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 789 1883 789
Size: 1094 3294
XmaI C'CCGG_G
Cuts at: 179 847 1034 1408 179
Size: 668 187 374 3159
Fragments arranged by size:
3159 668 374 187
XmnI GAAnn'nnTTC
Cuts at: 762 762
Size: 4388
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI
Bpu10I BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SspI StuI SwaI XhoI ZraI