Restriction map for
pF1KB7039
(Circular) (Six-base) MAPSORT of: pF1KB7039.seq Check: 9001 from: 1 to: 4487
pF1KB7039 4487 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1525 1525
Size: 4487
Acc65I G'GTAC_C
Cuts at: 1503 1503
Size: 4487
AclI AA'CG_TT
Cuts at: 4327 4327
Size: 4487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 113 2312 2744 3491 3759 113
Size: 2199 432 747 268 841
Fragments arranged by size:
2199 841 747 432 268
AflIII A'CryG_T
Cuts at: 1310 1839 2943 3920 1310
Size: 529 1104 977 1877
Fragments arranged by size:
1877 1104 977 529
AgeI A'CCGG_T
Cuts at: 2875 2875
Size: 4487
AhdI GACnn_n'nnGTC
Cuts at: 1226 1226
Size: 4487
AlwNI CAG_nnn'CTG
Cuts at: 447 1050 1792 3359 447
Size: 603 742 1567 1575
Fragments arranged by size:
1575 1567 742 603
ApaLI G'TGCA_C
Cuts at: 330 3257 330
Size: 2927 1560
ApoI r'AATT_y
Cuts at: 1491 3840 4096 1491
Size: 2349 256 1882
Fragments arranged by size:
2349 1882 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4487
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
AvaI C'yCGr_G
Cuts at: 868 1507 868
Size: 639 3848
BamHI G'GATC_C
Cuts at: 1512 1512
Size: 4487
BanI G'GyrC_C
Cuts at: 1503 1917 2165 2200 1503
Size: 414 248 35 3790
Fragments arranged by size:
3790 414 248 35
BanII G_rGCy'C
Cuts at: 353 1501 2531 353
Size: 1148 1030 2309
Fragments arranged by size:
2309 1148 1030
BbeI G_GCGC'C
Cuts at: 1921 2169 1921
Size: 248 4239
BbsI GAAGACnn'nnnn_
Cuts at: 569 569
Size: 4487
BciVI GTATCCnnnnn_n'
Cuts at: 2385 3146 4487 2385
Size: 761 1341 2385
Fragments arranged by size:
2385 1341 761
BclI T'GATC_A
Cuts at: 2007 2007
Size: 4487
BfrBI ATG'CAT
Cuts at: 909 909
Size: 4487
BglII A'GATC_T
Cuts at: 2002 4039 2002
Size: 2037 2450
BlpI GC'TnA_GC
Cuts at: 196 352 1599 196
Size: 156 1247 3084
Fragments arranged by size:
3084 1247 156
Bme1580I G_kGCm'C
Cuts at: 334 869 2112 2205 3261 334
Size: 535 1243 93 1056 1560
Fragments arranged by size:
1560 1243 1056 535 93
BmgBI CAC'GTC
Cuts at: 975 1338 975
Size: 363 4124
BmrI ACTGGGnnnn_n'
Cuts at: 1144 1699 1780 2115 4259 1144
Size: 555 81 335 2144 1372
Fragments arranged by size:
2144 1372 555 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 4487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 348 389 469 868 901 1000 189
Size: 159 41 80 399 33 99 3676
Fragments arranged by size:
3676 399 159 99 80 41 33
Bpu10I CC'TnA_GC
Cuts at: 289 1167 289
Size: 878 3609
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1663 2542 3034 3332 490
Size: 1173 879 492 298 1645
Fragments arranged by size:
1645 1173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 165 763 844 1369 4191 165
Size: 598 81 525 2822 461
Fragments arranged by size:
2822 598 525 461 81
BsaAI yAC'GTr
Cuts at: 1008 1311 1840 1008
Size: 303 529 3655
Fragments arranged by size:
3655 529 303
BsaBI GATnn'nnATC
Cuts at: 6 2001 4038 6
Size: 1995 2037 455
Fragments arranged by size:
2037 1995 455
BsaHI Gr'CG_yC
Cuts at: 1918 2166 2868 4364 1918
Size: 248 702 1496 2041
Fragments arranged by size:
2041 1496 702 248
BsaWI w'CCGG_w
Cuts at: 300 1676 2197 2875 3149 3296 3707 300
Size: 1376 521 678 274 147 411 1080
Fragments arranged by size:
1376 1080 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 241 271 1279 1309 241
Size: 30 1008 30 3419
Fragments arranged by size:
3419 1008 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 781 898 265
Size: 516 117 3854
Fragments arranged by size:
3854 516 117
BseYI C'CCAG_C
Cuts at: 207 237 999 1912 2670 3247 3743 207
Size: 30 762 913 758 577 496 951
Fragments arranged by size:
951 913 762 758 577 496 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 1129
Size: 4487
BsiEI CG_ry'CG
Cuts at: 104 651 2029 2075 3283 104
Size: 547 1378 46 1208 1308
Fragments arranged by size:
1378 1308 1208 547 46
BsiHKAI G_wGCw'C
Cuts at: 272 334 353 1501 2279 2469 3261 272
Size: 62 19 1148 778 190 792 1498
Fragments arranged by size:
1498 1148 792 778 190 62 19
BsmBI CGTCTCn'nnnn_
Cuts at: 1456 1456
Size: 4487
Bsp1286I G_dGCh'C
Cuts at: 272 334 353 869 1501 2112 2205 2279 2469
Size: 62 19 516 632 611 93 74 190
Cuts at: 2469 2531 3261 272
Size: 62 730 1498
Fragments arranged by size:
1498 730 632 611 516 190 93 74
62 62 19
BspEI T'CCGG_A
Cuts at: 3707 3707
Size: 4487
BspHI T'CATG_A
Cuts at: 1235 3663 1235
Size: 2428 2059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 293 1019 1151 1537 1751 2053 2434 293
Size: 726 132 386 214 302 381 2346
Fragments arranged by size:
2346 726 386 381 302 214 132
BsrBI CCG'CTC
Cuts at: 50 2779 2833 4317 4482 50
Size: 2729 54 1484 165 55
Fragments arranged by size:
2729 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2399 2399
Size: 4487
BsrFI r'CCGG_y
Cuts at: 1024 2485 2666 2875 1024
Size: 1461 181 209 2636
Fragments arranged by size:
2636 1461 209 181
BsrGI T'GTAC_A
Cuts at: 593 1110 593
Size: 517 3970
BssSI C'ACGA_G
Cuts at: 2758 3116 2758
Size: 358 4129
BstAPI GCAn_nnn'nTGC
Cuts at: 1864 1864
Size: 4487
BstBI TT'CG_AA
Cuts at: 2848 2848
Size: 4487
BstYI r'GATC_y
Cuts at: 1 983 1512 1979 2002 2337 2583 3595 3681
Size: 982 529 467 23 335 246 1012 86
Cuts at: 3681 3693 4039 1
Size: 12 346 449
Fragments arranged by size:
1012 982 529 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 344 1016 344
Size: 672 3815
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1344 2437 2618 2781 1344
Size: 1093 181 163 3050
Fragments arranged by size:
3050 1093 181 163
BtsI GCAGTG_nn'
Cuts at: 262 655 1423 1730 262
Size: 393 768 307 3019
Fragments arranged by size:
3019 768 393 307
DraI TTT'AAA
Cuts at: 1486 1486
Size: 4487
DraIII CAC_nnn'GTG
Cuts at: 329 798 1152 329
Size: 469 354 3664
Fragments arranged by size:
3664 469 354
DrdI GACnn_nn'nnGTC
Cuts at: 2193 3051 2193
Size: 858 3629
EaeI y'GGCC_r
Cuts at: 386 800 2072 2246 2637 2664 4009 386
Size: 414 1272 174 391 27 1345 864
Fragments arranged by size:
1345 1272 864 414 391 174 27
EagI C'GGCC_G
Cuts at: 2072 2072
Size: 4487
EarI CTCTTCn'nnn_
Cuts at: 496 665 868 2510 2720 496
Size: 169 203 1642 210 2263
Fragments arranged by size:
2263 1642 210 203 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 598 2695 3005 3151 4297 598
Size: 2097 310 146 1146 788
Fragments arranged by size:
2097 1146 788 310 146
EcoICRI GAG'CTC
Cuts at: 351 1499 351
Size: 1148 3339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 113 189 348 389 469 868 901 1000 2312
Size: 76 159 41 80 399 33 99 1312
Cuts at: 2312 2744 3491 3759 113
Size: 432 747 268 841
Fragments arranged by size:
1312 841 747 432 399 268 159 99
80 76 41 33
EcoNI CCTnn'n_nnAGG
Cuts at: 439 1252 439
Size: 813 3674
EcoO109I rG'GnC_Cy
Cuts at: 212 444 1047 1220 1626 212
Size: 232 603 173 406 3073
Fragments arranged by size:
3073 603 406 232 173
EcoRI G'AATT_C
Cuts at: 1491 1491
Size: 4487
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4487
FspI TGC'GCA
Cuts at: 754 2268 754
Size: 1514 2973
HaeII r_GCGC'y
Cuts at: 462 1921 2169 3191 4170 462
Size: 1459 248 1022 979 779
Fragments arranged by size:
1459 1022 979 779 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 645 677 1049 1081 1212 1244 1279 1311 2329
Size: 32 372 32 131 32 35 32 1018
Cuts at: 2329 2359 2361 2391 645
Size: 30 2 30 2741
Fragments arranged by size:
2741 1018 372 131 35 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 642 1526 642
Size: 884 3603
KasI G'GCGC_C
Cuts at: 1917 2165 1917
Size: 248 4239
KpnI G_GTAC'C
Cuts at: 1507 1507
Size: 4487
MluI A'CGCG_T
Cuts at: 3920 3920
Size: 4487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 398 1035 1295 1322 3158 3342 398
Size: 637 260 27 1836 184 1543
Fragments arranged by size:
1836 1543 637 260 184 27
MscI TGG'CCA
Cuts at: 388 802 2248 388
Size: 414 1446 2627
Fragments arranged by size:
2627 1446 414
MslI CAynn'nnrTG
Cuts at: 121 904 1249 2476 2603 121
Size: 783 345 1227 127 2005
Fragments arranged by size:
2005 1227 783 345 127
MspA1I CmG'CkG
Cuts at: 237 409 1293 1598 1912 2272 3285 3530 237
Size: 172 884 305 314 360 1013 245 1194
Fragments arranged by size:
1194 1013 884 360 314 305 245 172
NaeI GCC'GGC
Cuts at: 1026 2668 1026
Size: 1642 2845
NarI GG'CG_CC
Cuts at: 1918 2166 1918
Size: 248 4239
NgoMIV G'CCGG_C
Cuts at: 1024 2666 1024
Size: 1642 2845
NsiI A_TGCA'T
Cuts at: 911 911
Size: 4487
NspI r_CATG'y
Cuts at: 319 909 913 1540 2040 2947 319
Size: 590 4 627 500 907 1859
Fragments arranged by size:
1859 907 627 590 500 4
PciI A'CATG_T
Cuts at: 2943 2943
Size: 4487
PflMI CCAn_nnn'nTGG
Cuts at: 447 798 1314 447
Size: 351 516 3620
Fragments arranged by size:
3620 516 351
PfoI T'CCnGG_A
Cuts at: 845 845
Size: 4487
PmeI GTTT'AAAC
Cuts at: 1486 1486
Size: 4487
PmlI CAC'GTG
Cuts at: 1311 1311
Size: 4487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 4455
PpuMI rG'GwC_Cy
Cuts at: 212 1047 1220 212
Size: 835 173 3479
Fragments arranged by size:
3479 835 173
PsiI TTA'TAA
Cuts at: 1178 1178
Size: 4487
PstI C_TGCA'G
Cuts at: 1534 2219 1534
Size: 685 3802
PvuI CG_AT'CG
Cuts at: 104 651 104
Size: 547 3940
PvuII CAG'CTG
Cuts at: 1912 2272 1912
Size: 360 4127
RsrII CG'GwC_CG
Cuts at: 1295 2682 1295
Size: 1387 3100
SacI G_AGCT'C
Cuts at: 353 1501 353
Size: 1148 3339
SalI G'TCGA_C
Cuts at: 1524 1524
Size: 4487
SanDI GG'GwC_CC
Cuts at: 212 212
Size: 4487
SapI GCTCTTCn'nnn_
Cuts at: 496 868 2510 2720 496
Size: 372 1642 210 2263
Fragments arranged by size:
2263 1642 372 210
SbfI CC_TGCA'GG
Cuts at: 1534 1534
Size: 4487
ScaI AGT'ACT
Cuts at: 1414 4004 1414
Size: 2590 1897
SfcI C'TryA_G
Cuts at: 33 1530 2215 3208 3399 33
Size: 1497 685 993 191 1121
Fragments arranged by size:
1497 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1919 2167 1919
Size: 248 4239
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
SmaI CCC'GGG
Cuts at: 870 1509 870
Size: 639 3848
SmlI C'TyrA_G
Cuts at: 505 1642 2557 3049 3311 505
Size: 1137 915 492 262 1681
Fragments arranged by size:
1681 1137 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4487
SphI G_CATG'C
Cuts at: 319 909 913 1540 2040 319
Size: 590 4 627 500 2766
Fragments arranged by size:
2766 627 590 500 4
StuI AGG'CCT
Cuts at: 278 400 1344 278
Size: 122 944 3421
Fragments arranged by size:
3421 944 122
StyI C'CwwG_G
Cuts at: 395 734 1621 1975 395
Size: 339 887 354 2907
Fragments arranged by size:
2907 887 354 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 153 174 1163 2070 2203 2662 2871 153
Size: 21 989 907 133 459 209 1769
Fragments arranged by size:
1769 989 907 459 209 133 21
TatI w'GTAC_w
Cuts at: 593 923 1110 1369 1412 4002 593
Size: 330 187 259 43 2590 1078
Fragments arranged by size:
2590 1078 330 259 187 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 241 273 4004 4036 241
Size: 32 3731 32 692
Fragments arranged by size:
3731 692 32 32
Tth111I GACn'n_nGTC
Cuts at: 2284 2284
Size: 4487
XbaI T'CTAG_A
Cuts at: 1518 1518
Size: 4487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1982 1982
Size: 4487
XmaI C'CCGG_G
Cuts at: 868 1507 868
Size: 639 3848
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmtI BsiWI
BsmI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PshAI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI