Restriction map for
pF1KB7043
(Circular) (Six-base) MAPSORT of: pF1KB7043.seq Check: 1195 from: 1 to: 4523
pF1KB7043 4523 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1561 1561
Size: 4523
Acc65I G'GTAC_C
Cuts at: 1539 1539
Size: 4523
AclI AA'CG_TT
Cuts at: 4363 4363
Size: 4523
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1437 2348 2780 3527 3795 1437
Size: 911 432 747 268 2165
Fragments arranged by size:
2165 911 747 432 268
AflIII A'CryG_T
Cuts at: 1875 2979 3956 1875
Size: 1104 977 2442
Fragments arranged by size:
2442 1104 977
AgeI A'CCGG_T
Cuts at: 2911 2911
Size: 4523
AhdI GACnn_n'nnGTC
Cuts at: 410 410
Size: 4523
AlwNI CAG_nnn'CTG
Cuts at: 939 957 1828 3395 939
Size: 18 871 1567 2067
Fragments arranged by size:
2067 1567 871 18
ApaI G_GGCC'C
Cuts at: 807 1373 807
Size: 566 3957
ApaLI G'TGCA_C
Cuts at: 3293 3293
Size: 4523
ApoI r'AATT_y
Cuts at: 1527 3876 4132 1527
Size: 2349 256 1918
Fragments arranged by size:
2349 1918 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4523
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4523
AvaI C'yCGr_G
Cuts at: 247 397 445 595 1543 247
Size: 150 48 150 948 3227
Fragments arranged by size:
3227 948 150 150 48
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 478 511 1212 1245 478
Size: 33 701 33 3756
Fragments arranged by size:
3756 701 33 33
BamHI G'GATC_C
Cuts at: 1548 1548
Size: 4523
BanI G'GyrC_C
Cuts at: 384 393 1137 1161 1539 1953 2201 2236 384
Size: 9 744 24 378 414 248 35 2671
Fragments arranged by size:
2671 744 414 378 248 35 24 9
BanII G_rGCy'C
Cuts at: 248 807 1049 1373 1537 2567 248
Size: 559 242 324 164 1030 2204
Fragments arranged by size:
2204 1030 559 324 242 164
BbeI G_GCGC'C
Cuts at: 388 397 1957 2205 388
Size: 9 1560 248 2706
Fragments arranged by size:
2706 1560 248 9
BbsI GAAGACnn'nnnn_
Cuts at: 524 725 904 524
Size: 201 179 4143
Fragments arranged by size:
4143 201 179
BbvCI CC'TCA_GC
Cuts at: 882 882
Size: 4523
BciVI GTATCCnnnnn_n'
Cuts at: 2421 3182 4523 2421
Size: 761 1341 2421
Fragments arranged by size:
2421 1341 761
BclI T'GATC_A
Cuts at: 2043 2043
Size: 4523
BglII A'GATC_T
Cuts at: 2038 4075 2038
Size: 2037 2486
BlpI GC'TnA_GC
Cuts at: 1635 1635
Size: 4523
Bme1580I G_kGCm'C
Cuts at: 758 807 1118 1166 1373 2148 2241 3297 758
Size: 49 311 48 207 775 93 1056 1984
Fragments arranged by size:
1984 1056 775 311 207 93 49 48
BmgBI CAC'GTC
Cuts at: 1278 1278
Size: 4523
BmrI ACTGGGnnnn_n'
Cuts at: 1735 1816 2151 4295 1735
Size: 81 335 2144 1963
Fragments arranged by size:
2144 1963 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 897 2890 897
Size: 1993 2530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 1233 1300 179
Size: 1054 67 3402
Fragments arranged by size:
3402 1054 67
Bpu10I CC'TnA_GC
Cuts at: 882 882
Size: 4523
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1018 1234 1699 2578 3070 3368 835
Size: 183 216 465 879 492 298 1990
Fragments arranged by size:
1990 879 492 465 298 216 183
BsaI GGTCTCn'nnnn_
Cuts at: 1081 4227 1081
Size: 3146 1377
BsaAI yAC'GTr
Cuts at: 579 1876 579
Size: 1297 3226
BsaBI GATnn'nnATC
Cuts at: 6 2037 4074 6
Size: 2031 2037 455
Fragments arranged by size:
2037 2031 455
BsaHI Gr'CG_yC
Cuts at: 385 394 982 1954 2202 2904 4400 385
Size: 9 588 972 248 702 1496 508
Fragments arranged by size:
1496 972 702 588 508 248 9
BsaWI w'CCGG_w
Cuts at: 786 1712 2233 2911 3185 3332 3743 786
Size: 926 521 678 274 147 411 1566
Fragments arranged by size:
1566 926 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 805 813 805
Size: 8 4515
BseYI C'CCAG_C
Cuts at: 270 408 419 977 1048 1948 2706 3283 3779
Size: 138 11 558 71 900 758 577 496
Cuts at: 3779 270
Size: 1014
Fragments arranged by size:
1014 900 758 577 558 496 138 71
11
BsiEI CG_ry'CG
Cuts at: 104 799 841 2065 2111 3319 104
Size: 695 42 1224 46 1208 1308
Fragments arranged by size:
1308 1224 1208 695 46 42
BsiHKAI G_wGCw'C
Cuts at: 766 1265 1537 2315 2505 3297 766
Size: 499 272 778 190 792 1992
Fragments arranged by size:
1992 792 778 499 272 190
BsiWI C'GTAC_G
Cuts at: 377 377
Size: 4523
BsmBI CGTCTCn'nnnn_
Cuts at: 193 193
Size: 4523
Bsp1286I G_dGCh'C
Cuts at: 248 758 766 807 1049 1118 1166 1265 1373
Size: 510 8 41 242 69 48 99 108
Cuts at: 1373 1537 2148 2241 2315 2505 2567 3297 248
Size: 164 611 93 74 190 62 730 1474
Fragments arranged by size:
1474 730 611 510 242 190 164 108
99 93 74 69 62 48 41 8
BspEI T'CCGG_A
Cuts at: 786 3743 786
Size: 2957 1566
BspHI T'CATG_A
Cuts at: 3699 3699
Size: 4523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1573 1787 2089 2470 1573
Size: 214 302 381 3626
Fragments arranged by size:
3626 381 302 214
BsrBI CCG'CTC
Cuts at: 50 460 629 795 2815 2869 4353 4518 50
Size: 410 169 166 2020 54 1484 165 55
Fragments arranged by size:
2020 1484 410 169 166 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1436 2435 1436
Size: 999 3524
BsrFI r'CCGG_y
Cuts at: 840 1143 2521 2702 2911 840
Size: 303 1378 181 209 2452
Fragments arranged by size:
2452 1378 303 209 181
BsrGI T'GTAC_A
Cuts at: 1507 1507
Size: 4523
BssHII G'CGCG_C
Cuts at: 177 177
Size: 4523
BssSI C'ACGA_G
Cuts at: 759 2794 3152 759
Size: 2035 358 2130
Fragments arranged by size:
2130 2035 358
BstAPI GCAn_nnn'nTGC
Cuts at: 960 1900 960
Size: 940 3583
BstBI TT'CG_AA
Cuts at: 2884 2884
Size: 4523
BstYI r'GATC_y
Cuts at: 1 1064 1548 2015 2038 2373 2619 3631 3717
Size: 1063 484 467 23 335 246 1012 86
Cuts at: 3717 3729 4075 1
Size: 12 346 449
Fragments arranged by size:
1063 1012 484 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 188 188
Size: 4523
BtgI C'CryG_G
Cuts at: 107 328 482 526 683 107
Size: 221 154 44 157 3947
Fragments arranged by size:
3947 221 157 154 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 254 2473 2654 2817 254
Size: 2219 181 163 1960
Fragments arranged by size:
2219 1960 181 163
BtsI GCAGTG_nn'
Cuts at: 1105 1297 1766 1105
Size: 192 469 3862
Fragments arranged by size:
3862 469 192
DraI TTT'AAA
Cuts at: 1522 1522
Size: 4523
DrdI GACnn_nn'nnGTC
Cuts at: 2229 3087 2229
Size: 858 3665
EaeI y'GGCC_r
Cuts at: 604 796 838 2108 2282 2673 2700 4045 604
Size: 192 42 1270 174 391 27 1345 1082
Fragments arranged by size:
1345 1270 1082 391 192 174 42 27
EagI C'GGCC_G
Cuts at: 796 838 2108 796
Size: 42 1270 3211
Fragments arranged by size:
3211 1270 42
EarI CTCTTCn'nnn_
Cuts at: 2546 2756 2546
Size: 210 4313
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 294 1158 1162 2731 3041 3187 4333 294
Size: 864 4 1569 310 146 1146 484
Fragments arranged by size:
1569 1146 864 484 310 146 4
EcoICRI GAG'CTC
Cuts at: 1535 1535
Size: 4523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 1233 1300 1437 2348 2780 3527 3795 179
Size: 1054 67 137 911 432 747 268 907
Fragments arranged by size:
1054 911 907 747 432 268 137 67
EcoO109I rG'GnC_Cy
Cuts at: 803 804 1369 1370 1662 803
Size: 1 565 1 292 3664
Fragments arranged by size:
3664 565 292 1 1
EcoRI G'AATT_C
Cuts at: 1527 1527
Size: 4523
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4523
FspI TGC'GCA
Cuts at: 970 2304 970
Size: 1334 3189
HaeII r_GCGC'y
Cuts at: 292 388 397 595 667 691 850 1957 2205
Size: 96 9 198 72 24 159 1107 248
Cuts at: 2205 3227 4206 292
Size: 1022 979 609
Fragments arranged by size:
1107 1022 979 609 248 198 159 96
72 24 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 396 428 753 785 808 840 2365 2395 2397
Size: 32 325 32 23 32 1525 30 2
Cuts at: 2397 2427 396
Size: 30 2492
Fragments arranged by size:
2492 1525 325 32 32 32 30 30
23 2
HincII GTy'rAC
Cuts at: 1562 1562
Size: 4523
KasI G'GCGC_C
Cuts at: 384 393 1953 2201 384
Size: 9 1560 248 2706
Fragments arranged by size:
2706 1560 248 9
KpnI G_GTAC'C
Cuts at: 1543 1543
Size: 4523
MluI A'CGCG_T
Cuts at: 3956 3956
Size: 4523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3194 3378 3194
Size: 184 4339
MscI TGG'CCA
Cuts at: 2284 2284
Size: 4523
MslI CAynn'nnrTG
Cuts at: 157 1354 2512 2639 157
Size: 1197 1158 127 2041
Fragments arranged by size:
2041 1197 1158 127
MspA1I CmG'CkG
Cuts at: 302 500 528 701 886 957 1634 1948 2308
Size: 198 28 173 185 71 677 314 360
Cuts at: 2308 3321 3566 302
Size: 1013 245 1259
Fragments arranged by size:
1259 1013 677 360 314 245 198 185
173 71 28
NaeI GCC'GGC
Cuts at: 1145 2704 1145
Size: 1559 2964
NarI GG'CG_CC
Cuts at: 385 394 1954 2202 385
Size: 9 1560 248 2706
Fragments arranged by size:
2706 1560 248 9
NcoI C'CATG_G
Cuts at: 107 107
Size: 4523
NgoMIV G'CCGG_C
Cuts at: 1143 2702 1143
Size: 1559 2964
NspI r_CATG'y
Cuts at: 156 357 1056 1576 2076 2983 156
Size: 201 699 520 500 907 1696
Fragments arranged by size:
1696 907 699 520 500 201
PciI A'CATG_T
Cuts at: 2979 2979
Size: 4523
PflMI CCAn_nnn'nTGG
Cuts at: 957 1211 957
Size: 254 4269
PmeI GTTT'AAAC
Cuts at: 1522 1522
Size: 4523
PmlI CAC'GTG
Cuts at: 579 579
Size: 4523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 3662
Size: 32 4491
PspOMI G'GGCC_C
Cuts at: 803 1369 803
Size: 566 3957
PstI C_TGCA'G
Cuts at: 1061 1127 1292 1570 2255 1061
Size: 66 165 278 685 3329
Fragments arranged by size:
3329 685 278 165 66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4523
PvuII CAG'CTG
Cuts at: 957 1948 2308 957
Size: 991 360 3172
Fragments arranged by size:
3172 991 360
RsrII CG'GwC_CG
Cuts at: 2718 2718
Size: 4523
SacI G_AGCT'C
Cuts at: 1537 1537
Size: 4523
SacII CC_GC'GG
Cuts at: 529 529
Size: 4523
SalI G'TCGA_C
Cuts at: 1560 1560
Size: 4523
SapI GCTCTTCn'nnn_
Cuts at: 2546 2756 2546
Size: 210 4313
SbfI CC_TGCA'GG
Cuts at: 1570 1570
Size: 4523
ScaI AGT'ACT
Cuts at: 919 4040 919
Size: 3121 1402
SfcI C'TryA_G
Cuts at: 33 348 546 1057 1123 1288 1566 2251 3244
Size: 315 198 511 66 165 278 685 993
Cuts at: 3244 3435 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 511 315 278 198 191
165 66
SfoI GGC'GCC
Cuts at: 386 395 1955 2203 386
Size: 9 1560 248 2706
Fragments arranged by size:
2706 1560 248 9
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4523
SgrAI Cr'CCGG_yG
Cuts at: 1143 1143
Size: 4523
SmaI CCC'GGG
Cuts at: 249 447 1545 249
Size: 198 1098 3227
Fragments arranged by size:
3227 1098 198
SmlI C'TyrA_G
Cuts at: 850 997 1249 1678 2593 3085 3347 850
Size: 147 252 429 915 492 262 2026
Fragments arranged by size:
2026 915 492 429 262 252 147
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4523
SphI G_CATG'C
Cuts at: 357 1056 1576 2076 357
Size: 699 520 500 2804
Fragments arranged by size:
2804 699 520 500
StyI C'CwwG_G
Cuts at: 107 1232 1365 1657 2011 107
Size: 1125 133 292 354 2619
Fragments arranged by size:
2619 1125 354 292 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 341 2106 2239 2698 2907 341
Size: 1765 133 459 209 1957
Fragments arranged by size:
1957 1765 459 209 133
TatI w'GTAC_w
Cuts at: 917 1301 1507 4038 917
Size: 384 206 2531 1402
Fragments arranged by size:
2531 1402 384 206
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4040 4072 4040
Size: 32 4491
Tth111I GACn'n_nGTC
Cuts at: 2320 2320
Size: 4523
XbaI T'CTAG_A
Cuts at: 1554 1554
Size: 4523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 614 2018 614
Size: 1404 3119
XmaI C'CCGG_G
Cuts at: 247 445 1543 247
Size: 198 1098 3227
Fragments arranged by size:
3227 1098 198
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BcgI BfrBI BglI BmtI BsaXI BsgI BsmI
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PpuMI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI